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P26453 (BASI_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Basigin
Alternative name(s):
Glycoprotein CE9
OX-47 antigen
CD_antigen=CD147
Gene names
Name:Bsg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes By similarity.

Subunit structure

Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface By similarity. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with AJAP1, ATP1B2, MAG and L1CAM By similarity. Ref.5 Ref.7

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Melanosome By similarity. Note: Colocalizes with SLC16A1 and SLC16A8 By similarity.

Tissue specificity

Essentially found in lymphocytes. Also present in various immature cells, endothelia and cells with excitable membranes.

Developmental stage

In uterus during peri-implantation period strongly expressed in the luminal epithelium on day 1 of pregnancy and gradually decreased to a basal concentration from day 3 to day 5 of pregnancy. Strongly expressed in the implanting blastocyst and primary decidua on day 6 of pregnancy. Ref.6

Induction

By activation of lymphocytes by mitogens. By estrogen in the uterine epithelium of ovariectomized animals. Ref.6

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P26453-1)

Also known as: Basigin-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P26453-2)

The sequence of this isoform differs from the canonical sequence as follows:
     25-140: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 388367Basigin
PRO_0000014521

Regions

Topological domain22 – 324303Extracellular Potential
Transmembrane325 – 34824Helical; Potential
Topological domain349 – 38840Cytoplasmic Potential
Domain138 – 21982Ig-like C2-type
Domain221 – 320100Ig-like V-type

Amino acid modifications

Modified residue3571Phosphothreonine By similarity
Glycosylation1601N-linked (GlcNAc...) By similarity
Glycosylation2691N-linked (GlcNAc...) By similarity
Glycosylation3051N-linked (GlcNAc...) Potential
Disulfide bond157 ↔ 203 By similarity
Disulfide bond242 ↔ 304 By similarity

Natural variations

Alternative sequence25 – 140116Missing in isoform 2.
VSP_011503

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Basigin-2) [UniParc].

Last modified August 31, 2004. Version 2.
Checksum: 3D655169F537E3D4

FASTA38842,436
        10         20         30         40         50         60 
MAAALLLALA FTFLSGQGAC AAAGFLKAPM SQEQWAGGSV VLHCEAVGSP MPEIQWWFEG 

        70         80         90        100        110        120 
NEPNDSCSQL WDGARLDRVH IHATYRQHAA STLSVDGLAA EDTGTYECRA SSDPDRNHLT 

       130        140        150        160        170        180 
RPPRVKWVRA QASVVVLEPG TIVTSVQEVD SKTQLTCFLN SSGIDIVGHR WMRGGKVLQE 

       190        200        210        220        230        240 
DTLPDLQMKY TVDADDRSGE YSCIFLPEPV GRGNINVEGP PRIKVGKKSE HASEGEFVKL 

       250        260        270        280        290        300 
ICKSEASHPP VDEWVWFKTS DTGDQTISNG TEANSKYVII STPELSELII SDLDMNVDPG 

       310        320        330        340        350        360 
TYVCNATNSQ GSARETISLR VRSRLAALWP FLGIVAEVLV LVTIIFIYEK RRKPDQTLDE 

       370        380 
DDPGAAPLKG SGSHLNDKDK NVRQRNAT

« Hide

Isoform 2 [UniParc].

Checksum: AB2B589ABEAB0B0D
Show »

FASTA27229,585

References

[1]"The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence."
Fossum S., Mallet S., Barclay A.N.
Eur. J. Immunol. 21:671-679(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: PVG X DA.
[2]"Breaching the diffusion barrier that compartmentalizes the transmembrane glycoprotein CE9 to the posterior-tail plasma membrane domain of the rat spermatozoon."
Nehme C.L., Cesario M.M., Myles D.G., Koppel D.E., Bartles J.R.
J. Cell Biol. 120:687-694(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"Retina-specific expression of 5A11/Basigin-2, a member of the immunoglobulin gene superfamily."
Ochrietor J.D., Moroz T.P., van Ekeris L., Clamp M.F., Jefferson S.C., deCarvalho A.C., Fadool J.M., Wistow G., Muramatsu T., Linser P.J.
Invest. Ophthalmol. Vis. Sci. 44:4086-4096(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Sprague-Dawley.
Tissue: Retina.
[4]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 177-222; 228-239; 244-276; 315-320 AND 352-369, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[5]"CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression."
Kirk P., Wilson M.C., Heddle C., Brown M.H., Barclay A.N., Halestrap A.P.
EMBO J. 19:3896-3904(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLC16A1 AND SLC1A3.
[6]"Basigin expression and hormonal regulation in the rat uterus during the peri-implantation period."
Xiao L.J., Diao H.L., Ma X.H., Ding N.Z., Kadomatsu K., Muramatsu T., Yang Z.M.
Reproduction 124:219-225(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, INDUCTION.
[7]"Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT1 and CD147 provide information on the topology and stoichiometry of the complex in situ."
Wilson M.C., Meredith D., Halestrap A.P.
J. Biol. Chem. 277:3666-3672(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLC16A1, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X54640 mRNA. Translation: CAA38452.1.
X67215 mRNA. Translation: CAA47655.1.
AY120888 mRNA. Translation: AAM81604.1.
IPIIPI00193425.
IPI00382166.
PIRS15674.
RefSeqNP_001103352.1. NM_001109882.1.
NP_036915.1. NM_012783.3.
UniGeneRn.2269.

3D structure databases

ProteinModelPortalP26453.
ModBaseSearch...

Protein-protein interaction databases

IntActP26453. 1 interaction.
MINTMINT-4996604.

PTM databases

PhosphoSiteP26453.

Proteomic databases

PaxDbP26453.
PRIDEP26453.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011275; ENSRNOP00000011275; ENSRNOG00000008414.
ENSRNOT00000044275; ENSRNOP00000050252; ENSRNOG00000008414.
GeneID25246.
KEGGrno:25246.
UCSCRGD:2220. rat.

Organism-specific databases

CTD682.
RGD2220. Bsg.

Phylogenomic databases

eggNOGNOG83713.
GeneTreeENSGT00390000010516.
HOGENOMHOG000263411.
HOVERGENHBG008120.
InParanoidP26453.
KOK06535.
OMADRNHLTR.
OrthoDBEOG49KFR2.

Gene expression databases

ArrayExpressP26453.
GenevestigatorP26453.
GermOnlineENSRNOG00000008414. Rattus norvegicus.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
InterProIPR009151. Basigin.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PANTHERPTHR10075:SF6. PTHR10075:SF6. 1 hit.
PfamPF07679. I-set. 2 hits.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio605849.

Entry information

Entry nameBASI_RAT
AccessionPrimary (citable) accession number: P26453
Secondary accession number(s): Q7TNP1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 31, 2004
Last modified: April 3, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents