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Protein

Mitotic check point protein BUB2

Gene

BUB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the GTP-binding protein TEM1 by the BFA1/BUB2 complex.

GO - Molecular functioni

  • GTPase activator activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle orientation checkpoint Source: SGD
  • negative regulation of protein localization to mitotic spindle pole body Source: SGD
  • positive regulation of GTPase activity Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic check point protein BUB2
Alternative name(s):
Cell cycle arrest protein BUB2
Gene namesi
Name:BUB2
Ordered Locus Names:YMR055C
ORF Names:YM9796.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR055c.
EuPathDBiFungiDB:YMR055C.
SGDiS000004659. BUB2.

Subcellular locationi

GO - Cellular componenti

  • Bfa1-Bub2 complex Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Mitotic check point protein BUB2PRO_0000208008Add
BLAST

Proteomic databases

MaxQBiP26448.

Interactioni

Subunit structurei

Interacts with BFA1.

Binary interactionsi

WithEntry#Exp.IntActNotes
BFA1P471135EBI-3824,EBI-3586
GIC1P387855EBI-3824,EBI-7575
TEM1P389872EBI-3824,EBI-19113

Protein-protein interaction databases

BioGridi35231. 137 interactions.
DIPiDIP-1215N.
IntActiP26448. 33 interactions.
MINTiMINT-395099.

Structurei

3D structure databases

ProteinModelPortaliP26448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 235199Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the BUB2 family.Curated
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5210.
HOGENOMiHOG000212290.
InParanoidiP26448.
KOiK02179.
OMAiRLNDAHE.
OrthoDBiEOG7WQ83F.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL
60 70 80 90 100
SQTSMEASTQ RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED
110 120 130 140 150
ALIRCLSCFA WQTQQRRQKT RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM
160 170 180 190 200
AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL LDISLRIIDP KLSKFLSDNL
210 220 230 240 250
LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI LFVVAFLVKM
260 270 280 290 300
RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD

PDIYIP
Length:306
Mass (Da):35,028
Last modified:August 1, 1992 - v1
Checksum:iA1DDBFB548E81EA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64706 Genomic DNA. Translation: AAA16885.1.
Z49703 Genomic DNA. Translation: CAA89765.1.
BK006946 Genomic DNA. Translation: DAA09954.1.
PIRiA39654.
RefSeqiNP_013771.1. NM_001182553.1.

Genome annotation databases

EnsemblFungiiYMR055C; YMR055C; YMR055C.
GeneIDi855077.
KEGGisce:YMR055C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64706 Genomic DNA. Translation: AAA16885.1.
Z49703 Genomic DNA. Translation: CAA89765.1.
BK006946 Genomic DNA. Translation: DAA09954.1.
PIRiA39654.
RefSeqiNP_013771.1. NM_001182553.1.

3D structure databases

ProteinModelPortaliP26448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35231. 137 interactions.
DIPiDIP-1215N.
IntActiP26448. 33 interactions.
MINTiMINT-395099.

Proteomic databases

MaxQBiP26448.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR055C; YMR055C; YMR055C.
GeneIDi855077.
KEGGisce:YMR055C.

Organism-specific databases

CYGDiYMR055c.
EuPathDBiFungiDB:YMR055C.
SGDiS000004659. BUB2.

Phylogenomic databases

eggNOGiCOG5210.
HOGENOMiHOG000212290.
InParanoidiP26448.
KOiK02179.
OMAiRLNDAHE.
OrthoDBiEOG7WQ83F.

Enzyme and pathway databases

BioCyciYEAST:G3O-32760-MONOMER.

Miscellaneous databases

NextBioi978357.
PROiP26448.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function."
    Hoyt M.A., Totis L., Roberts B.T.
    Cell 66:507-517(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
    Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
    J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiBUB2_YEAST
AccessioniPrimary (citable) accession number: P26448
Secondary accession number(s): D6VZN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 22, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.