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P26448

- BUB2_YEAST

UniProt

P26448 - BUB2_YEAST

Protein

Mitotic check point protein BUB2

Gene

BUB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the GTP-binding protein TEM1 by the BFA1/BUB2 complex.

    GO - Molecular functioni

    1. GTPase activator activity Source: SGD
    2. protein binding Source: IntAct
    3. Rab GTPase activator activity Source: InterPro

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. mitotic spindle orientation checkpoint Source: SGD
    3. positive regulation of GTPase activity Source: GOC
    4. regulation of exit from mitosis Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32760-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitotic check point protein BUB2
    Alternative name(s):
    Cell cycle arrest protein BUB2
    Gene namesi
    Name:BUB2
    Ordered Locus Names:YMR055C
    ORF Names:YM9796.08C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR055c.
    SGDiS000004659. BUB2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 306306Mitotic check point protein BUB2PRO_0000208008Add
    BLAST

    Proteomic databases

    MaxQBiP26448.

    Expressioni

    Gene expression databases

    GenevestigatoriP26448.

    Interactioni

    Subunit structurei

    Interacts with BFA1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BFA1P471135EBI-3824,EBI-3586
    GIC1P387855EBI-3824,EBI-7575
    TEM1P389872EBI-3824,EBI-19113

    Protein-protein interaction databases

    BioGridi35231. 136 interactions.
    DIPiDIP-1215N.
    IntActiP26448. 33 interactions.
    MINTiMINT-395099.
    STRINGi4932.YMR055C.

    Structurei

    3D structure databases

    ProteinModelPortaliP26448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 235199Rab-GAP TBCPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the BUB2 family.Curated
    Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5210.
    HOGENOMiHOG000212290.
    KOiK02179.
    OMAiRLNDAHE.
    OrthoDBiEOG7WQ83F.

    Family and domain databases

    InterProiIPR000195. Rab-GTPase-TBC_dom.
    [Graphical view]
    PfamiPF00566. RabGAP-TBC. 1 hit.
    [Graphical view]
    SMARTiSM00164. TBC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47923. SSF47923. 2 hits.
    PROSITEiPS50086. TBC_RABGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26448-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL    50
    SQTSMEASTQ RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED 100
    ALIRCLSCFA WQTQQRRQKT RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM 150
    AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL LDISLRIIDP KLSKFLSDNL 200
    LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI LFVVAFLVKM 250
    RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD 300
    PDIYIP 306
    Length:306
    Mass (Da):35,028
    Last modified:August 1, 1992 - v1
    Checksum:iA1DDBFB548E81EA3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64706 Genomic DNA. Translation: AAA16885.1.
    Z49703 Genomic DNA. Translation: CAA89765.1.
    BK006946 Genomic DNA. Translation: DAA09954.1.
    PIRiA39654.
    RefSeqiNP_013771.1. NM_001182553.1.

    Genome annotation databases

    EnsemblFungiiYMR055C; YMR055C; YMR055C.
    GeneIDi855077.
    KEGGisce:YMR055C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64706 Genomic DNA. Translation: AAA16885.1 .
    Z49703 Genomic DNA. Translation: CAA89765.1 .
    BK006946 Genomic DNA. Translation: DAA09954.1 .
    PIRi A39654.
    RefSeqi NP_013771.1. NM_001182553.1.

    3D structure databases

    ProteinModelPortali P26448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35231. 136 interactions.
    DIPi DIP-1215N.
    IntActi P26448. 33 interactions.
    MINTi MINT-395099.
    STRINGi 4932.YMR055C.

    Proteomic databases

    MaxQBi P26448.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR055C ; YMR055C ; YMR055C .
    GeneIDi 855077.
    KEGGi sce:YMR055C.

    Organism-specific databases

    CYGDi YMR055c.
    SGDi S000004659. BUB2.

    Phylogenomic databases

    eggNOGi COG5210.
    HOGENOMi HOG000212290.
    KOi K02179.
    OMAi RLNDAHE.
    OrthoDBi EOG7WQ83F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32760-MONOMER.

    Miscellaneous databases

    NextBioi 978357.
    PROi P26448.

    Gene expression databases

    Genevestigatori P26448.

    Family and domain databases

    InterProi IPR000195. Rab-GTPase-TBC_dom.
    [Graphical view ]
    Pfami PF00566. RabGAP-TBC. 1 hit.
    [Graphical view ]
    SMARTi SM00164. TBC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47923. SSF47923. 2 hits.
    PROSITEi PS50086. TBC_RABGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function."
      Hoyt M.A., Totis L., Roberts B.T.
      Cell 66:507-517(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
      Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
      J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiBUB2_YEAST
    AccessioniPrimary (citable) accession number: P26448
    Secondary accession number(s): D6VZN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3