P26448 (BUB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitotic check point protein BUB2

Alternative name(s):
Cell cycle arrest protein BUB2

Gene names

Name:	BUB2
Ordered Locus Names:	YMR055C
ORF Names:	YM9796.08C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the GTP-binding protein TEM1 by the BFA1/BUB2 complex.
Subunit structure	Interacts with BFA1.
Subcellular location	Cytoplasm › cytoskeleton › spindle pole.
Sequence similarities	Belongs to the BUB2 family. Contains 1 Rab-GAP TBC domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle orientation checkpoint Inferred from mutant phenotype PubMed 16039591. Source: SGD positive regulation of Rab GTPase activity Inferred from electronic annotation. Source: GOC regulation of exit from mitosis Traceable author statement PubMed 16039591. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW spindle pole body Inferred from direct assay PubMed 10220406PubMed 10352016. Source: SGD
Molecular_function	GTPase activator activity Traceable author statement PubMed 12432084. Source: SGD Rab GTPase activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BFA1	P47113	5	EBI-3824,EBI-3586
GIC1	P38785	5	EBI-3824,EBI-7575
TEM1	P38987	2	EBI-3824,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Mitotic check point protein BUB2		PRO_0000208008
Regions
	Domain	37 – 235	199	Rab-GAP TBC

Sequences

	Sequence		Length	Mass (Da)
	P26448 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: A1DDBFB548E81EA3	FASTA	306	35,028
	10 20 30 40 50 60 MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL SQTSMEASTQ 70 80 90 100 110 120 RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED ALIRCLSCFA WQTQQRRQKT 130 140 150 160 170 180 RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL 190 200 210 220 230 240 LDISLRIIDP KLSKFLSDNL LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI 250 260 270 280 290 300 LFVVAFLVKM RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD PDIYIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function." Hoyt M.A., Totis L., Roberts B.T. Cell 66:507-517(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis." Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H. J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64706 Genomic DNA. Translation: AAA16885.1. Z49703 Genomic DNA. Translation: CAA89765.1. BK006946 Genomic DNA. Translation: DAA09954.1.
PIR	A39654.
RefSeq	NP_013771.1. NM_001182553.1.
3D structure databases
ProteinModelPortal	P26448.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1215N.
IntAct	P26448. 33 interactions.
MINT	MINT-395099.
STRING	4932.YMR055C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR055C; YMR055C; YMR055C.
GeneID	855077.
KEGG	sce:YMR055C.
Organism-specific databases
CYGD	YMR055c.
SGD	S000004659. BUB2.
Phylogenomic databases
eggNOG	COG5210.
HOGENOM	HOG000212290.
KO	K02179.
OMA	QGMNVLC.
OrthoDB	EOG4C5GTB.
Gene expression databases
Genevestigator	P26448.
GermOnline	YMR055C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000195. Rab-GTPase-TBC_dom. [Graphical view]
Pfam	PF00566. RabGAP-TBC. 1 hit. [Graphical view]
SMART	SM00164. TBC. 1 hit. [Graphical view]
SUPFAM	SSF47923. RabGAP_TBC. 2 hits.
PROSITE	PS50086. TBC_RABGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978357.

Entry information

Entry name

BUB2_YEAST

Accession

Primary (citable) accession number: P26448
Secondary accession number(s): D6VZN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program