P26447 (S10A4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A4

Alternative name(s):
Calvasculin
Metastasin
Placental calcium-binding protein
Protein Mts1
S100 calcium-binding protein A4

Gene names

Name:	S100A4
Synonyms:	CAPL, MTS1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode. Ref.10
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	epithelial to mesenchymal transition Traceable author statement PubMed 14679171. Source: HGNC positive regulation of I-kappaB kinase/NF-kappaB signaling Inferred from direct assay PubMed 15033494. Source: UniProtKB
Cellular_component	extracellular space Inferred from direct assay PubMed 22664934PubMed 23580065. Source: UniProt extracellular vesicular exosome Inferred from direct assay PubMed 19056867PubMed 23376485. Source: UniProt neuron projection Inferred from electronic annotation. Source: Ensembl nucleus Inferred from direct assay PubMed 12118070PubMed 15033494. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay PubMed 15033494. Source: UniProtKB
Molecular_function	RAGE receptor binding Inferred from physical interaction PubMed 15033494. Source: UniProtKB calcium ion binding Traceable author statement PubMed 8204608. Source: ProtInc poly(A) RNA binding Inferred from direct assay PubMed 22658674. Source: UniProtKB protein binding Inferred from physical interaction PubMed 14640694PubMed 15479433. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AREGB	P15514	4	EBI-717058,EBI-953674
BTC	P35070	2	EBI-717058,EBI-6590057
MDM2	Q00987	3	EBI-717058,EBI-389668
TP53	P04637	3	EBI-717058,EBI-366083

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.11

Chain

2 – 101

100

Protein S100-A4

PRO_0000143977

Regions

Domain

12 – 47

36

EF-hand 1

Domain

50 – 85

36

EF-hand 2

Calcium binding

20 – 33

14

1; low affinity Potential

Calcium binding

63 – 74

12

2; high affinity Potential

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.11

Modified residue

7

1

N6-acetyllysine Ref.11

Modified residue

35

1

N6-acetyllysine Ref.11

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Helix

4 – 20

17

Beta strand

22 – 25

4

Helix

31 – 41

11

Helix

43 – 46

4

Helix

52 – 62

11

Beta strand

64 – 66

3

Beta strand

67 – 71

5

Helix

72 – 88

17

Turn

89 – 91

3

Sequences

	Sequence		Length	Mass (Da)
	P26447 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 286D2B7B07EDB562	FASTA	101	11,729
	10 20 30 40 50 60 MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS 70 80 90 100 NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart." Engelkamp D., Schaefer B.W., Erne P., Heizmann C.W. Biochemistry 31:10258-10264(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E." Engelkamp D., Schaefer B.W., Mattei M.-G., Erne P., Heizmann C.W. Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Transcriptional analysis of the mts1 gene with specific reference to 5' flanking sequences." Tulchinsky E.M., Ford H.L., Kramerov D., Reshetnyak E., Grigorian M., Zain S., Lukanidin E. Proc. Natl. Acad. Sci. U.S.A. 89:9146-9150(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Spleen.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Synovium.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix and Prostate.
[9]	"Calcyclin and calvasculin exist in human platelets." Tomida Y., Terasawa M., Kobayashi R., Hidaka H. Biochem. Biophys. Res. Commun. 189:1310-1316(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-18 AND 36-57. Tissue: Platelet.
[10]	"Liprin beta 1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, is a new target for the metastasis-associated protein S100A4 (Mts1)." Kriajevska M., Fischer-Larsen M., Moertz E., Vorm O., Tulchinsky E., Grigorian M., Ambartsumian N., Lukanidin E. J. Biol. Chem. 277:5229-5235(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PPFIBP1.
[11]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2; LYS-7 AND LYS-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Solution structure of human Mts1 (S100A4) as determined by NMR spectroscopy." Vallely K.M., Rustandi R.R., Ellis K.C., Varlamova O., Bresnick A.R., Weber D.J. Biochemistry 41:12670-12680(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M80563 mRNA. Translation: AAA51920.1.
Z18950 Genomic DNA. Translation: CAA79474.1.
Z33457 Genomic DNA. Translation: CAA83880.1.
CR450345 mRNA. Translation: CAG29341.1.
AK292083 mRNA. Translation: BAF84772.1.
BX470102 Genomic DNA. Translation: CAI14754.1.
CH471121 Genomic DNA. Translation: EAW53310.1.
CH471121 Genomic DNA. Translation: EAW53311.1.
CH471121 Genomic DNA. Translation: EAW53312.1.
CH471121 Genomic DNA. Translation: EAW53313.1.
CH471121 Genomic DNA. Translation: EAW53314.1.
CH471121 Genomic DNA. Translation: EAW53315.1.
BC000838 mRNA. Translation: AAH00838.1.
BC016300 mRNA. Translation: AAH16300.1.

CCDS

CCDS1042.1.

PIR

A48219.

RefSeq

NP_002952.1. NM_002961.2.
NP_062427.1. NM_019554.2.

UniGene

Hs.654444.

3D structure databases

PDBe
RCSB-PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M31	NMR	-	A/B	1-101	[»]
2LNK	NMR	-	A/B	1-101	[»]
2Q91	X-ray	1.63	A/B	1-101	[»]
3C1V	X-ray	1.50	A/B/C/D	1-101	[»]
3CGA	X-ray	2.03	A/B	1-101	[»]
3KO0	X-ray	2.30	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	1-101	[»]
3M0W	X-ray	2.80	A/B/C/D/E/F/G/H/I/J	2-101	[»]
3ZWH	X-ray	1.94	A/B	1-101	[»]
4CFQ	X-ray	1.37	A/B/C/D	1-88	[»]
4CFR	X-ray	1.40	A/B	1-101	[»]
4ETO	X-ray	1.54	A/B	1-93	[»]
4HSZ	X-ray	2.25	A/B/C/D	1-93	[»]

ProteinModelPortal

P26447.

SMR

P26447. Positions 2-98.

ModBase

Search...

MobiDB

Search...

Protein-protein interaction databases

BioGrid

112183. 19 interactions.

DIP

DIP-44938N.

IntAct

P26447. 11 interactions.

MINT

MINT-1372269.

STRING

9606.ENSP00000346294.

Chemistry

ChEMBL

CHEMBL2362976.

PTM databases

PhosphoSite

P26447.

Polymorphism databases

DMDM

115601.

2D gel databases

DOSAC-COBS-2DPAGE

P26447.

SWISS-2DPAGE

P26447.

Proteomic databases

MaxQB

P26447.

PaxDb

P26447.

PeptideAtlas

P26447.

PRIDE

P26447.

Protocols and materials databases

DNASU

6275.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000354332; ENSP00000346294; ENSG00000196154.
ENST00000368714; ENSP00000357703; ENSG00000196154.
ENST00000368715; ENSP00000357704; ENSG00000196154.
ENST00000368716; ENSP00000357705; ENSG00000196154.

GeneID

6275.

KEGG

hsa:6275.

UCSC

uc001fby.3. human.

Organism-specific databases

CTD

6275.

GeneCards

GC01M153517.

HGNC

HGNC:10494. S100A4.

HPA

CAB002618.
CAB027387.
CAB058698.
HPA007973.

MIM

114210. gene.

neXtProt

NX_P26447.

PharmGKB

PA34906.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG40931.

HOGENOM

HOG000246968.

HOVERGEN

HBG001479.

InParanoid

P26447.

OMA

CVAMMCN.

OrthoDB

EOG7R833W.

PhylomeDB

P26447.

TreeFam

TF332727.

Gene expression databases

Bgee

P26447.

CleanEx

HS_S100A4.

Genevestigator

P26447.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028496. S100-A4.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

PANTHER

PTHR11639:SF15. PTHR11639:SF15. 1 hit.

Pfam

PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

S100A4. human.

EvolutionaryTrace

P26447.

GeneWiki

S100A4.

GenomeRNAi

6275.

NextBio

24355.

PRO

P26447.

SOURCE

Search...

Entry information

Entry name

S10A4_HUMAN

Accession

Primary (citable) accession number: P26447
Secondary accession number(s): A8K7R8, D3DV46, Q6ICP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	July 9, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.