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P26447 (S10A4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein S100-A4
Alternative name(s):
Calvasculin
Metastasin
Placental calcium-binding protein
Protein Mts1
S100 calcium-binding protein A4
Gene names
Name:S100A4
Synonyms:CAPL, MTS1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length101 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode. Ref.10

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the S-100 family.

Contains 2 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.11
Chain2 – 101100Protein S100-A4
PRO_0000143977

Regions

Domain12 – 4736EF-hand 1
Domain50 – 8536EF-hand 2
Calcium binding20 – 33141; low affinity Potential
Calcium binding63 – 74122; high affinity Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.11
Modified residue71N6-acetyllysine Ref.11
Modified residue351N6-acetyllysine Ref.11

Secondary structure

................ 101
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P26447 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 286D2B7B07EDB562

FASTA10111,729
        10         20         30         40         50         60 
MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS 

        70         80         90        100 
NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K 

« Hide

References

« Hide 'large scale' references
[1]"S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart."
Engelkamp D., Schaefer B.W., Erne P., Heizmann C.W.
Biochemistry 31:10258-10264(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E."
Engelkamp D., Schaefer B.W., Mattei M.-G., Erne P., Heizmann C.W.
Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Transcriptional analysis of the mts1 gene with specific reference to 5' flanking sequences."
Tulchinsky E.M., Ford H.L., Kramerov D., Reshetnyak E., Grigorian M., Zain S., Lukanidin E.
Proc. Natl. Acad. Sci. U.S.A. 89:9146-9150(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Spleen.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Synovium.
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix and Prostate.
[9]"Calcyclin and calvasculin exist in human platelets."
Tomida Y., Terasawa M., Kobayashi R., Hidaka H.
Biochem. Biophys. Res. Commun. 189:1310-1316(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 8-18 AND 36-57.
Tissue: Platelet.
[10]"Liprin beta 1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, is a new target for the metastasis-associated protein S100A4 (Mts1)."
Kriajevska M., Fischer-Larsen M., Moertz E., Vorm O., Tulchinsky E., Grigorian M., Ambartsumian N., Lukanidin E.
J. Biol. Chem. 277:5229-5235(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPFIBP1.
[11]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2; LYS-7 AND LYS-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Solution structure of human Mts1 (S100A4) as determined by NMR spectroscopy."
Vallely K.M., Rustandi R.R., Ellis K.C., Varlamova O., Bresnick A.R., Weber D.J.
Biochemistry 41:12670-12680(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80563 mRNA. Translation: AAA51920.1.
Z18950 Genomic DNA. Translation: CAA79474.1.
Z33457 Genomic DNA. Translation: CAA83880.1.
CR450345 mRNA. Translation: CAG29341.1.
AK292083 mRNA. Translation: BAF84772.1.
BX470102 Genomic DNA. Translation: CAI14754.1.
CH471121 Genomic DNA. Translation: EAW53310.1.
CH471121 Genomic DNA. Translation: EAW53311.1.
CH471121 Genomic DNA. Translation: EAW53312.1.
CH471121 Genomic DNA. Translation: EAW53313.1.
CH471121 Genomic DNA. Translation: EAW53314.1.
CH471121 Genomic DNA. Translation: EAW53315.1.
BC000838 mRNA. Translation: AAH00838.1.
BC016300 mRNA. Translation: AAH16300.1.
CCDSCCDS1042.1.
PIRA48219.
RefSeqNP_002952.1. NM_002961.2.
NP_062427.1. NM_019554.2.
UniGeneHs.654444.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M31NMR-A/B1-101[»]
2LNKNMR-A/B1-101[»]
2Q91X-ray1.63A/B1-101[»]
3C1VX-ray1.50A/B/C/D1-101[»]
3CGAX-ray2.03A/B1-101[»]
3KO0X-ray2.30A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-101[»]
3M0WX-ray2.80A/B/C/D/E/F/G/H/I/J2-101[»]
3ZWHX-ray1.94A/B1-101[»]
4CFQX-ray1.37A/B/C/D1-88[»]
4CFRX-ray1.40A/B1-101[»]
4ETOX-ray1.54A/B1-93[»]
4HSZX-ray2.25A/B/C/D1-93[»]
ProteinModelPortalP26447.
SMRP26447. Positions 2-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112183. 19 interactions.
DIPDIP-44938N.
IntActP26447. 11 interactions.
MINTMINT-1372269.
STRING9606.ENSP00000346294.

Chemistry

ChEMBLCHEMBL2362976.

PTM databases

PhosphoSiteP26447.

Polymorphism databases

DMDM115601.

2D gel databases

DOSAC-COBS-2DPAGEP26447.
SWISS-2DPAGEP26447.

Proteomic databases

MaxQBP26447.
PaxDbP26447.
PeptideAtlasP26447.
PRIDEP26447.

Protocols and materials databases

DNASU6275.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354332; ENSP00000346294; ENSG00000196154.
ENST00000368714; ENSP00000357703; ENSG00000196154.
ENST00000368715; ENSP00000357704; ENSG00000196154.
ENST00000368716; ENSP00000357705; ENSG00000196154.
GeneID6275.
KEGGhsa:6275.
UCSCuc001fby.3. human.

Organism-specific databases

CTD6275.
GeneCardsGC01M153517.
HGNCHGNC:10494. S100A4.
HPACAB002618.
CAB027387.
CAB058698.
HPA007973.
MIM114210. gene.
neXtProtNX_P26447.
PharmGKBPA34906.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40931.
HOGENOMHOG000246968.
HOVERGENHBG001479.
InParanoidP26447.
OMACVAMMCN.
OrthoDBEOG7R833W.
PhylomeDBP26447.
TreeFamTF332727.

Gene expression databases

BgeeP26447.
CleanExHS_S100A4.
GenevestigatorP26447.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028496. S100-A4.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERPTHR11639:SF15. PTHR11639:SF15. 1 hit.
PfamPF01023. S_100. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSS100A4. human.
EvolutionaryTraceP26447.
GeneWikiS100A4.
GenomeRNAi6275.
NextBio24355.
PROP26447.
SOURCESearch...

Entry information

Entry nameS10A4_HUMAN
AccessionPrimary (citable) accession number: P26447
Secondary accession number(s): A8K7R8, D3DV46, Q6ICP8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM