P26441 (CNTF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ciliary neurotrophic factor
Short name=CNTF

Gene names

Name:

CNTF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Nervous system.
Sequence similarities	Belongs to the CNTF family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Developmental protein Growth factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ciliary neurotrophic factor-mediated signaling pathway Inferred from direct assay PubMed 12643274. Source: BHF-UCL growth Inferred from electronic annotation. Source: InterPro muscle organ morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of neuron apoptotic process Inferred from direct assay PubMed 10966616. Source: BHF-UCL negative regulation of photoreceptor cell differentiation Inferred from electronic annotation. Source: Compara neuron development Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from direct assay PubMed 12643274. Source: BHF-UCL regulation of retinal cell programmed cell death Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred by curator PubMed 12643274. Source: BHF-UCL intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA
Molecular_function	ciliary neurotrophic factor receptor binding Traceable author statement PubMed 1714745. Source: ProtInc growth factor activity Inferred from direct assay PubMed 12643274. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF3E	P60228	1	EBI-1050897,EBI-347740
EXT1	Q16394	1	EBI-1050897,EBI-1046007
EXT2	Q93063	1	EBI-1050897,EBI-1047761
ICA1	Q05084	1	EBI-1050897,EBI-1046751
STK16	O75716	1	EBI-1050897,EBI-749295
VHL	P40337	1	EBI-1050897,EBI-301246

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Ciliary neurotrophic factor

PRO_0000149519

Natural variations

Natural variant

49

1

N → S.
Corresponds to variant rs17152779 [ dbSNP | Ensembl ].

VAR_033777

Natural variant

182

1

H → R. Ref.10
Corresponds to variant rs6266 [ dbSNP | Ensembl ].

VAR_013924

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

200

Helix Strand Turn

Details...

Helix

13 – 41

29

Helix

69 – 95

27

Turn

96 – 98

3

Beta strand

99 – 101

3

Helix

104 – 129

26

Beta strand

138 – 140

3

Helix

152 – 180

29

Sequences

	Sequence		Length	Mass (Da)
	P26441 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: EDD9D895BDA6F35A	FASTA	200	22,931
	10 20 30 40 50 60 MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS 70 80 90 100 110 120 TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA 130 140 150 160 170 180 YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS 190 200 SHQTGIPARG SHYIANNKKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Recombinant human and rat ciliary neurotrophic factors." Masiakowski P., Liu H., Radziejewski C., Lottspeich F., Oberthuer W., Wong V., Lindsay R.M., Furth M.E., Panayotatos N. J. Neurochem. 57:1003-1012(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and expression of human ciliary neurotrophic factor." Negro A., Tolosano E., Skaper S., Martini I., Callegaro L., Silengo L., Fiorini F., Altruda F. Eur. J. Biochem. 201:289-294(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence and structural organization of the human gene encoding ciliary neurotrophic factor." Lam A., Fuller F., Miller J., Kloss J., Manthorpe M., Varon M., Cordell B. Gene 102:271-276(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lung.
[4]	"Expression and characterization of recombinant human ciliary neurotrophic factor from Escherichia coli." McDonald J.R., Ko C., Mismer D., Smith D.J., Collins F. Biochim. Biophys. Acta 1090:70-80(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"A null mutation in the human CNTF gene is not causally related to neurological diseases." Takahashi R., Yokoji H., Misawa H., Hayashi M., Hu J., Deguchi T. Nat. Genet. 7:79-84(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Sciatic nerve.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[9]	"Crystal structure of dimeric human ciliary neurotrophic factor determined by MAD phasing." McDonald N.Q., Panayotatos N., Hendrickson W.A. EMBO J. 14:2689-2699(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-187.
[10]	"Characterization of single-nucleotide polymorphisms in coding regions of human genes." Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT ARG-182.
[11]	Erratum Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 23:373-373(1999)
+	Additional computationally mapped references.

Web resources

Wikipedia

Ciliary neurotrophic factor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X60542 Genomic DNA. Translation: CAA43032.1.
X60477, X60478 Genomic DNA. Translation: CAA43009.1.
X55889, X55890 Genomic DNA. Translation: CAA39374.1.
AK314118 mRNA. Translation: BAG36809.1.
CH471076 Genomic DNA. Translation: EAW73819.1.
BC068030 mRNA. Translation: AAH68030.1.
BC074963 mRNA. Translation: AAH74963.1.
BC074964 mRNA. Translation: AAH74964.1.

IPI

IPI00032298.

PIR

UNHUCF. JH0455.

RefSeq

NP_000605.1. NM_000614.3.

UniGene

Hs.524920.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CNT	X-ray	2.40	1/2/3/4	1-187	[»]

ProteinModelPortal

P26441.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5776N.

MINT

MINT-205057.

STRING

9606.ENSP00000355370.

PTM databases

PhosphoSite

P26441.

Polymorphism databases

DMDM

116585.

Proteomic databases

PaxDb

P26441.

PRIDE

P26441.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361987; ENSP00000355370; ENSG00000242689.

GeneID

1270.

KEGG

hsa:1270.

UCSC

uc001nna.4. human.

Organism-specific databases

CTD

1270.

GeneCards

GC11P058390.

HGNC

HGNC:2169. CNTF.

HPA

HPA019654.

MIM

118945. gene.

neXtProt

NX_P26441.

PharmGKB

PA26683.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG114783.

HOGENOM

HOG000059605.

HOVERGEN

HBG005362.

KO

K05420.

OMA

KLWGLKV.

OrthoDB

EOG4M65JM.

Enzyme and pathway databases

SignaLink

P26441.

Gene expression databases

Bgee

P26441.

CleanEx

HS_CNTF.

Genevestigator

P26441.

GermOnline

ENSG00000205003. Homo sapiens.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000151. Ciliary_neurotrophic_fac_CNTF.
[Graphical view]

Pfam

PF01110. CNTF. 1 hit.
[Graphical view]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P26441.

GenomeRNAi

1270.

NextBio

5143.

SOURCE

Search...

Entry information

Entry name

CNTF_HUMAN

Accession

Primary (citable) accession number: P26441
Secondary accession number(s): B2RAB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 29, 2013
This is version 140 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.