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P26431

- SL9A1_RAT

UniProt

P26431 - SL9A1_RAT

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Protein

Sodium/hydrogen exchanger 1

Gene

Slc9a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei165 – 1651Channel pore-liningBy similarity

GO - Molecular functioni

  1. calcium-dependent protein binding Source: UniProtKB
  2. sodium:proton antiporter activity Source: UniProtKB

GO - Biological processi

  1. cardiac muscle cell contraction Source: RGD
  2. cardiac muscle cell differentiation Source: Ensembl
  3. cell differentiation Source: RGD
  4. cell growth Source: RGD
  5. cellular response to acidic pH Source: UniProtKB
  6. cellular response to antibiotic Source: RGD
  7. cellular response to electrical stimulus Source: RGD
  8. cellular response to hypoxia Source: RGD
  9. cellular response to insulin stimulus Source: RGD
  10. cellular response to organic cyclic compound Source: RGD
  11. negative regulation of apoptotic process Source: RGD
  12. neuron death Source: RGD
  13. positive regulation of action potential Source: RGD
  14. positive regulation of apoptotic process Source: RGD
  15. positive regulation of cell growth Source: RGD
  16. positive regulation of intracellular signal transduction Source: RGD
  17. positive regulation of mitochondrial membrane permeability Source: RGD
  18. regulation of intracellular pH Source: UniProtKB
  19. regulation of pH Source: UniProtKB
  20. regulation of sensory perception of pain Source: RGD
  21. regulation of the force of heart contraction Source: RGD
  22. response to acidic pH Source: UniProtKB
  23. response to drug Source: RGD
  24. response to organic cyclic compound Source: RGD
  25. sodium ion export Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Sodium

Enzyme and pathway databases

ReactomeiREACT_198825. Hyaluronan uptake and degradation.

Protein family/group databases

TCDBi2.A.36.1.1. the monovalent cation:proton antiporter-1 (cpa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 1
Alternative name(s):
Na(+)/H(+) exchanger 1
Short name:
NHE-1
Solute carrier family 9 member 1
Gene namesi
Name:Slc9a1
Synonyms:Nhe1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi3718. Slc9a1.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Note: Colocalizes with and CHP2 at the plasma membrane (By similarity). Colocalizes with CHP1 at the reticulum endoplasmic and plasma membrane.By similarity

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. basolateral plasma membrane Source: RGD
  3. cell surface Source: RGD
  4. cytoplasm Source: UniProtKB
  5. endoplasmic reticulum Source: UniProtKB
  6. extracellular vesicular exosome Source: Ensembl
  7. integral component of membrane Source: UniProtKB-KW
  8. intercalated disc Source: RGD
  9. membrane raft Source: RGD
  10. mitochondrion Source: RGD
  11. nucleus Source: Ensembl
  12. perinuclear region of cytoplasm Source: RGD
  13. plasma membrane Source: UniProtKB
  14. sarcolemma Source: RGD
  15. T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi522 – 53817INEEI…LLTGI → QNEEQHTQQLDHQQTGQ: Abolishes interaction with TESC and markedly reduces transporter activity. Does not alter its cell membrane localization. 1 PublicationAdd
BLAST
Mutagenesisi530 – 5356FLDHLL → AADHAA: Abolishes interaction with TESC and markedly reduces transporter activity. Does not alter its cell membrane localization. 1 Publication
Mutagenesisi530 – 5356FLDHLL → QQDHQQ: Abolishes interaction with TESC and markedly reduces transporter activity. Does not alter its cell membrane localization. 1 Publication
Mutagenesisi530 – 5356FLDHLL → RRDHRR: Abolishes interaction with TESC and markedly reduces transporter activity. Does not alter its cell membrane localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 820820Sodium/hydrogen exchanger 1PRO_0000052351Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Modified residuei603 – 6031PhosphoserineBy similarity
Modified residuei609 – 6091PhosphoserineBy similarity
Modified residuei697 – 6971PhosphoserineBy similarity
Modified residuei701 – 7011PhosphoserineBy similarity
Modified residuei707 – 7071PhosphoserineBy similarity
Modified residuei727 – 7271PhosphoserineBy similarity
Modified residuei730 – 7301PhosphoserineBy similarity
Modified residuei733 – 7331PhosphoserineBy similarity
Modified residuei790 – 7901PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated and O-glycosylated in the N-terminal region.2 Publications
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP26431.
PRIDEiP26431.

PTM databases

PhosphoSiteiP26431.

Expressioni

Tissue specificityi

Not tissue specific.

Gene expression databases

GenevestigatoriP26431.

Interactioni

Subunit structurei

Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner (By similarity). Oligomer. Interacts with CHP1, CHP2 and TESC.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Chp2Q810D12EBI-77471,EBI-6146708

Protein-protein interaction databases

IntActiP26431. 3 interactions.
STRINGi10116.ENSRNOP00000011049.

Structurei

3D structure databases

ProteinModelPortaliP26431.
SMRiP26431. Positions 159-184, 254-279, 342-369, 507-549.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini34 – 10572ExtracellularSequence AnalysisAdd
BLAST
Topological domaini132 – 1332CytoplasmicSequence Analysis
Topological domaini154 – 1585ExtracellularSequence Analysis
Topological domaini179 – 19517CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini216 – 23116ExtracellularSequence AnalysisAdd
BLAST
Topological domaini252 – 2609CytoplasmicSequence Analysis
Topological domaini281 – 29818ExtracellularSequence AnalysisAdd
BLAST
Topological domaini320 – 34223CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini363 – 38523ExtracellularSequence AnalysisAdd
BLAST
Topological domaini407 – 4148ExtracellularSequence Analysis
Topological domaini435 – 45218CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini475 – 4839ExtracellularSequence Analysis
Topological domaini504 – 820317CytoplasmicSequence AnalysisAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei386 – 40621Name=H10By similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321Helical; Name=M1Sequence AnalysisAdd
BLAST
Transmembranei106 – 13126Helical; Name=M2Sequence AnalysisAdd
BLAST
Transmembranei134 – 15320Helical; Name=M3Sequence AnalysisAdd
BLAST
Transmembranei159 – 17820Helical; Name=M4Sequence AnalysisAdd
BLAST
Transmembranei196 – 21520Helical; Name=M5Sequence AnalysisAdd
BLAST
Transmembranei232 – 25120Helical; Name=M6Sequence AnalysisAdd
BLAST
Transmembranei261 – 28020Helical; Name=M7Sequence AnalysisAdd
BLAST
Transmembranei299 – 31921Helical; Name=M8Sequence AnalysisAdd
BLAST
Transmembranei343 – 36220Helical; Name=M9Sequence AnalysisAdd
BLAST
Transmembranei415 – 43420Helical; Name=M10Sequence AnalysisAdd
BLAST
Transmembranei453 – 47422Helical; Name=M11Sequence AnalysisAdd
BLAST
Transmembranei484 – 50320Helical; Name=M12Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni520 – 54324Interaction with CHP2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0025.
GeneTreeiENSGT00760000119074.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiP26431.
KOiK05742.
OMAiYDRVGIV.
OrthoDBiEOG7XWPN4.
PhylomeDBiP26431.
TreeFamiTF317212.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

P26431-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMLRWSGIWG LYPPRIFPSL LVVVALVGLL PVLRSHGLQL NPTASTIRGS
60 70 80 90 100
EPPRERSIGD VTTAPSEPLH HPDDRNLTNL YIEHGAKPVR KAFPVLDIDY
110 120 130 140 150
LHVRTPFEIS LWILLACLMK IGFHVIPTIS SIVPESCLLI VVGLLVGGLI
160 170 180 190 200
KGVGETPPFL QSDVFFLFLL PPIILDAGYF LPLRQFTENL GTILIFAVVG
210 220 230 240 250
TLWNAFFLGG LLYAVCLVGG EQINNIGLLD TLLFGSIISA VDPVAVLAVF
260 270 280 290 300
EEIHINELLH ILVFGESLLN DAVTVVLYHL FEEFASYEYV GISDIFLGFL
310 320 330 340 350
SFFVVSLGGV FVGVVYGVIA AFTSRFTSHI RVIEPLFVFL YSYMAYLSAE
360 370 380 390 400
LFHLSGIMAL IASGVVMRPY VEANISHKSH TTIKYFLKMW SSVSETLIFI
410 420 430 440 450
FLGVSTVAGS HQWNWTFVIS TLLFCLIARV LGVLVLTWFI NKFRIVKLTP
460 470 480 490 500
KDQFIIAYGG LRGAIAFSLG YLLDKKHFPM CDLFLTAIIT VIFFTVFVQG
510 520 530 540 550
MTIRPLVDLL AVKKKQETKR SINEEIHTQF LDHLLTGIED ICGHYGHHHW
560 570 580 590 600
KDKLNRFNKK YVKKCLIAGE RSKEPQLIAF YHKMEMKQAI ELVESGGMGK
610 620 630 640 650
IPSAVSTVSM QNIHPKSAAS ERILPALSKD KEEEIRKILR SNLQKTRQRL
660 670 680 690 700
RSYNRHTLVA DPYEEAWNQM LLRRQKARQL EQKITNYLTV PAHKLDSPTM
710 720 730 740 750
SRARIGSDPL AYEPKADLPV ITIDPASPQS PESVDLVNEE LKGKVLGLKR
760 770 780 790 800
GPRTTPEEEE EDEDGVIMIR SKEPSSPGTD DVFTPGPSDS PGSQRIQRCL
810 820
SDPGPHPEPG EGEPFIPKGQ
Length:820
Mass (Da):91,647
Last modified:October 1, 1996 - v2
Checksum:i58398DE74A9642FB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85299 mRNA. Translation: AAA98479.1.
PIRiA40204.
RefSeqiNP_036784.1. NM_012652.1.
UniGeneiRn.5025.

Genome annotation databases

EnsembliENSRNOT00000011049; ENSRNOP00000011049; ENSRNOG00000007982.
GeneIDi24782.
KEGGirno:24782.
UCSCiRGD:3718. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85299 mRNA. Translation: AAA98479.1 .
PIRi A40204.
RefSeqi NP_036784.1. NM_012652.1.
UniGenei Rn.5025.

3D structure databases

ProteinModelPortali P26431.
SMRi P26431. Positions 159-184, 254-279, 342-369, 507-549.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P26431. 3 interactions.
STRINGi 10116.ENSRNOP00000011049.

Chemistry

BindingDBi P26431.
ChEMBLi CHEMBL2577.

Protein family/group databases

TCDBi 2.A.36.1.1. the monovalent cation:proton antiporter-1 (cpa1) family.

PTM databases

PhosphoSitei P26431.

Proteomic databases

PaxDbi P26431.
PRIDEi P26431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000011049 ; ENSRNOP00000011049 ; ENSRNOG00000007982 .
GeneIDi 24782.
KEGGi rno:24782.
UCSCi RGD:3718. rat.

Organism-specific databases

CTDi 6548.
RGDi 3718. Slc9a1.

Phylogenomic databases

eggNOGi COG0025.
GeneTreei ENSGT00760000119074.
HOGENOMi HOG000247044.
HOVERGENi HBG052615.
InParanoidi P26431.
KOi K05742.
OMAi YDRVGIV.
OrthoDBi EOG7XWPN4.
PhylomeDBi P26431.
TreeFami TF317212.

Enzyme and pathway databases

Reactomei REACT_198825. Hyaluronan uptake and degradation.

Miscellaneous databases

NextBioi 604392.
PROi P26431.

Gene expression databases

Genevestigatori P26431.

Family and domain databases

InterProi IPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
[Graphical view ]
PANTHERi PTHR10110. PTHR10110. 1 hit.
Pfami PF00999. Na_H_Exchanger. 1 hit.
[Graphical view ]
PRINTSi PR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsi TIGR00840. b_cpa1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins."
    Orlowski J., Kandasamy R.A., Shull G.E.
    J. Biol. Chem. 267:9331-9339(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  2. "Topological analysis of NHE1, the ubiquitous Na+/H+ exchanger using chymotryptic cleavage."
    Shrode L.D., Gan B.S., D'Souza S.J., Orlowski J., Grinstein S.
    Am. J. Physiol. 275:C431-C439(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.
  3. "Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium."
    Inoue H., Nakamura Y., Nagita M., Takai T., Masuda M., Nakamura N., Kanazawa H.
    Biol. Pharm. Bull. 26:148-155(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHP1 AND CHP2.
  4. "Calcineurin B homologous protein 3 promotes the biosynthetic maturation, cell surface stability, and optimal transport of the Na+/H+ exchanger NHE1 isoform."
    Zaun H.C., Shrier A., Orlowski J.
    J. Biol. Chem. 283:12456-12467(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TESC, MUTAGENESIS OF 530-PHE--LEU-535.
  5. "Dual functional significance of calcineurin homologous protein 1 binding to Na(+)/H(+) exchanger isoform 1."
    Matsushita M., Tanaka H., Mitsui K., Kanazawa H.
    Am. J. Physiol. 301:C280-C288(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: OLIGOMERIZATION, GLYCOSYLATION, UBIQUITINATION, MUTAGENESIS OF 522-ILE--ILE-538.

Entry informationi

Entry nameiSL9A1_RAT
AccessioniPrimary (citable) accession number: P26431
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.Curated
Although PubMed:18321853 show that TESC-binding results in the maturation and accumulation of SLC9A1 at the cell surface, previous studies with human SLC9A1 report that TESC-binding results in a decrease in activity.Curated
Although PubMed:18321853 show that they CHP1 and TESC bind at the same C-terminal site, previous studies with human SLC9A1 report that they bind to different sites.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3