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Protein

Hydrogenase transcriptional regulatory protein hupR1

Gene

hupR1

Organism
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi193 – 200ATPPROSITE-ProRule annotation8
Nucleotide bindingi255 – 264ATPPROSITE-ProRule annotation10
DNA bindingi452 – 471H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase transcriptional regulatory protein hupR1
Gene namesi
Name:hupR1
OrganismiRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifieri1061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811111 – 492Hydrogenase transcriptional regulatory protein hupR1Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei554-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi272942.RCAP_rcc00778.

Structurei

Secondary structure

1492
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 25Combined sources11
Turni26 – 28Combined sources3
Beta strandi31 – 36Combined sources6
Helixi37 – 46Combined sources10
Beta strandi49 – 56Combined sources8
Beta strandi59 – 61Combined sources3
Helixi63 – 73Combined sources11
Beta strandi77 – 84Combined sources8
Helixi89 – 97Combined sources9
Beta strandi102 – 107Combined sources6
Helixi110 – 140Combined sources31

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JK1X-ray2.10A5-140[»]
2VUHX-ray2.50B5-140[»]
2VUIX-ray2.90B5-140[»]
ProteinModelPortaliP26408.
SMRiP26408.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26408.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 121Response regulatoryPROSITE-ProRule annotationAdd BLAST115
Domaini164 – 393Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST230

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASAPAILL VDDEPHSLAA MKLALEDDFD VLTAQGAEAA IAILEEEWVQ
60 70 80 90 100
VIICDQRMPG RTGVDFLTEV RERWPETVRI IITGYTDSAS MMAAINDAGI
110 120 130 140 150
HQFLTKPWHP EQLLSSARNA ARMFTLAREN ERLSLEMRLL NSTSESRVEK
160 170 180 190 200
RRRALREGMG FETILRTPNS AMTGAIALAR QFASFDVPVL LRGEPGSGRA
210 220 230 240 250
QLARAMHYVS LRSDKPFYEI NLAGLPEDLA MIELFGARRG VLPGGVAKIG
260 270 280 290 300
LAQKADRGTL FVAGVEAASP ALQLALLRML ADGAITPLGG QETASTNLRL
310 320 330 340 350
ITGAAADLRA MVAEGRFRAD LYYALSAGEI ALPPLRARRG DVALLAQSML
360 370 380 390 400
AEAAVRHGKQ ALGFDAAALE FLENYDWPGN LRELHNEVTR MLIFAQDNVL
410 420 430 440 450
GAELISRHIL QAAPSESGAD RSAEEVMTAD GTLKDRIELI EMRILRETLT
460 470 480 490
RNRWNKSRAA AELGLSRVGL RAKLDRYGIE HPAGRVQEEE ED
Length:492
Mass (Da):53,838
Last modified:August 1, 1992 - v1
Checksum:i5BCF1ADCFE83A28A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63670 Genomic DNA. Translation: AAA26104.1.
X61007 Genomic DNA. Translation: CAA43327.1.
M55089 Genomic DNA. Translation: AAA72927.1.
PIRiS32951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63670 Genomic DNA. Translation: AAA26104.1.
X61007 Genomic DNA. Translation: CAA43327.1.
M55089 Genomic DNA. Translation: AAA72927.1.
PIRiS32951.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JK1X-ray2.10A5-140[»]
2VUHX-ray2.50B5-140[»]
2VUIX-ray2.90B5-140[»]
ProteinModelPortaliP26408.
SMRiP26408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272942.RCAP_rcc00778.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.

Miscellaneous databases

EvolutionaryTraceiP26408.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUPR_RHOCA
AccessioniPrimary (citable) accession number: P26408
Secondary accession number(s): Q6LDK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.