P26405 (RFBK_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphomannomutase
Short name=PMM
EC=5.4.2.8

Gene names

Name:	rfbK
Ordered Locus Names:	STM2083

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen.
Catalytic activity	Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the phosphohexose mutase family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway O antigen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphomannomutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Phosphomannomutase		PRO_0000147826
Regions
	Transmembrane	30 – 46	17	Helical; Potential
	Transmembrane	265 – 284	20	Helical; Potential
Sites
	Active site	111	1	Phosphoserine intermediate By similarity
	Metal binding	111	1	Magnesium; via phosphate group By similarity
	Metal binding	245	1	Magnesium By similarity
	Metal binding	247	1	Magnesium By similarity
	Metal binding	249	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26405 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: BA6BF851AB931915	FASTA	477	52,086
	10 20 30 40 50 60 MNVVNNSRDV IYSSGIVFGT SGARGLVKDF TPQVCAAFTV SFVAVMQEHF SFDTVALAID 70 80 90 100 110 120 NRPSSYGMAQ ACAAALADKG VNCIFYGVVP TPALAFQSMS DNMPAIMVTG SHIPFERNGL 130 140 150 160 170 180 KFYRPDGEIT KHDEAAILSV EDTCSHLELK ELIVSEMAAV NYISRYTSLF STPFLKNKRI 190 200 210 220 230 240 GIYEHSSAGR DLYKPLFIAL GAEVVSLGRS DNFVPIDTEA VSKEDREKAR SWAKEFDLDA 250 260 270 280 290 300 IFSTDGDGDR PLIADEAGEW LRGDILGLLC SLALDAEAVA IPVSCNSIIS SGRFFKHVKL 310 320 330 340 350 360 TKIGSPYVIE AFNELSRSYS RIVGFEANGG FLLGSDICIN EQNLHALPTR DAVLPAIMLL 370 380 390 400 410 420 YKSRNTSISA LVNELPTRYT HSDRLQGITT DKSQSLISMG RENLSNLLSY IGLENEGAIS 430 440 450 460 470 TDMTDGMRIT LRDGCIVHLR ASGNAPELRC YAEANLLNRA QDLVNTTLAN IKKRCLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)." Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R. Mol. Microbiol. 5:695-713(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56793 Genomic DNA. Translation: CAA40129.1. AE006468 Genomic DNA. Translation: AAL20987.1.
PIR	S15313.
RefSeq	NP_461028.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P26405.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2083.
Proteomic databases
PaxDb	P26405.
PRIDE	P26405.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20987; AAL20987; STM2083.
GeneID	1253604.
KEGG	stm:STM2083.
PATRIC	32382749. VBISalEnt20916_2205.
Phylogenomic databases
eggNOG	COG1109.
HOGENOM	HOG000066295.
KO	K01840.
OMA	RPSSYAM.
ProtClustDB	CLSK894265.
Enzyme and pathway databases
UniPathway	UPA00126; UER00424. UPA00281.
Family and domain databases
Gene3D	3.40.120.10. 1 hit.
InterPro	IPR005844. A-D-PHexomutase_a/b/a-I. IPR016055. A-D-PHexomutase_a/b/a-I/II/III. IPR005845. A-D-PHexomutase_a/b/a-II. IPR005846. A-D-PHexomutase_a/b/a-III. IPR005843. A-D-PHexomutase_C. IPR016066. A-D-PHexomutase_CS. [Graphical view]
Pfam	PF02878. PGM_PMM_I. 1 hit. PF02879. PGM_PMM_II. 1 hit. PF02880. PGM_PMM_III. 1 hit. PF00408. PGM_PMM_IV. 1 hit. [Graphical view]
SUPFAM	SSF53738. A-D-PHexomutase_a/b/a-I/II/III. 3 hits.
PROSITE	PS00710. PGM_PMM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFBK_SALTY

Accession

Primary (citable) accession number: P26405

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program