Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphomannomutase

Gene

rfbK

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen.

Catalytic activityi

Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei111 – 1111Phosphoserine intermediateBy similarity
Metal bindingi111 – 1111Magnesium; via phosphate groupBy similarity
Metal bindingi245 – 2451MagnesiumBy similarity
Metal bindingi247 – 2471MagnesiumBy similarity
Metal bindingi249 – 2491MagnesiumBy similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. phosphomannomutase activity Source: UniProtKB-EC

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  2. O antigen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2095-MONOMER.
UniPathwayiUPA00126; UER00424.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomannomutase (EC:5.4.2.8)
Short name:
PMM
Gene namesi
Name:rfbK
Ordered Locus Names:STM2083
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 4617HelicalSequence AnalysisAdd
BLAST
Transmembranei265 – 28420HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477PhosphomannomutasePRO_0000147826Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP26405.
PRIDEiP26405.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM2083.

Structurei

3D structure databases

ProteinModelPortaliP26405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphohexose mutase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1109.
HOGENOMiHOG000066295.
KOiK01840.
OMAiPVSCNTA.
OrthoDBiEOG6TFCNH.
PhylomeDBiP26405.

Family and domain databases

Gene3Di3.30.310.50. 1 hit.
3.40.120.10. 1 hit.
InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view]
PfamiPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
SUPFAMiSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEiPS00710. PGM_PMM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVVNNSRDV IYSSGIVFGT SGARGLVKDF TPQVCAAFTV SFVAVMQEHF
60 70 80 90 100
SFDTVALAID NRPSSYGMAQ ACAAALADKG VNCIFYGVVP TPALAFQSMS
110 120 130 140 150
DNMPAIMVTG SHIPFERNGL KFYRPDGEIT KHDEAAILSV EDTCSHLELK
160 170 180 190 200
ELIVSEMAAV NYISRYTSLF STPFLKNKRI GIYEHSSAGR DLYKPLFIAL
210 220 230 240 250
GAEVVSLGRS DNFVPIDTEA VSKEDREKAR SWAKEFDLDA IFSTDGDGDR
260 270 280 290 300
PLIADEAGEW LRGDILGLLC SLALDAEAVA IPVSCNSIIS SGRFFKHVKL
310 320 330 340 350
TKIGSPYVIE AFNELSRSYS RIVGFEANGG FLLGSDICIN EQNLHALPTR
360 370 380 390 400
DAVLPAIMLL YKSRNTSISA LVNELPTRYT HSDRLQGITT DKSQSLISMG
410 420 430 440 450
RENLSNLLSY IGLENEGAIS TDMTDGMRIT LRDGCIVHLR ASGNAPELRC
460 470
YAEANLLNRA QDLVNTTLAN IKKRCLL
Length:477
Mass (Da):52,086
Last modified:August 1, 1992 - v1
Checksum:iBA6BF851AB931915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56793 Genomic DNA. Translation: CAA40129.1.
AE006468 Genomic DNA. Translation: AAL20987.1.
PIRiS15313.
RefSeqiNP_461028.1. NC_003197.1.
WP_001103643.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20987; AAL20987; STM2083.
GeneIDi1253604.
KEGGistm:STM2083.
PATRICi32382749. VBISalEnt20916_2205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56793 Genomic DNA. Translation: CAA40129.1.
AE006468 Genomic DNA. Translation: AAL20987.1.
PIRiS15313.
RefSeqiNP_461028.1. NC_003197.1.
WP_001103643.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP26405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2083.

Proteomic databases

PaxDbiP26405.
PRIDEiP26405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20987; AAL20987; STM2083.
GeneIDi1253604.
KEGGistm:STM2083.
PATRICi32382749. VBISalEnt20916_2205.

Phylogenomic databases

eggNOGiCOG1109.
HOGENOMiHOG000066295.
KOiK01840.
OMAiPVSCNTA.
OrthoDBiEOG6TFCNH.
PhylomeDBiP26405.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00424.
UPA00281.
BioCyciSENT99287:GCTI-2095-MONOMER.

Family and domain databases

Gene3Di3.30.310.50. 1 hit.
3.40.120.10. 1 hit.
InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view]
PfamiPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
SUPFAMiSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEiPS00710. PGM_PMM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)."
    Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R.
    Mol. Microbiol. 5:695-713(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiRFBK_SALTY
AccessioniPrimary (citable) accession number: P26405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: January 7, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.