Reviewed, UniProtKB/Swiss-Prot P26404 (RFBM_SALTY)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanylyltransferase rfbM
    EC=2.7.7.13
Alternative name(s):
    GDP-mannose pyrophosphorylase
      Short name=GMPP
      Short name=GMP
    GTP--mannose-1-phosphate guanylyltransferase

Gene names

Name:	rfbM
Ordered Locus Names:	STM2084

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose. Ref.3
Pathway	Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; GDP-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Subunit structure	Homodimer. Ref.3
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.
Biophysicochemical properties	Kinetic parameters: KM=0.01 mM for mannose-1-phosphate KM=0.20 mM for GTP Vmax=1.80 µmol/min/mg enzyme toward mannose-1-phosphate Vmax=2.40 µmol/min/mg enzyme toward GTP

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Mannose-1-phosphate guanylyltransferase rfbM		PRO_0000194261

Sequences

	Sequence		Length	Mass (Da)
	P26404-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: FE9E84D5CEE1F118	FASTA	479	54,046
	10 20 30 40 50 60 MSFLPVIMAG GTGSRLWPLS REYHPKQFLS VEGKLSMLQN TIKRLASLST EEPVVICNDR 70 80 90 100 110 120 HRFLVAEQLR EIDKLANNII LEPVGRNTAP AIALAAFCAL QNADNADPLL LVLAADHVIQ 130 140 150 160 170 180 DEIAFTKAVR HAEEYAANGK LVTFGIVPTH AETGYGYIRR GELIGNDAYA VAEFVEKPDI 190 200 210 220 230 240 DTAGDYFKSG KYYWNSGMFL FRASSYLNEL KYLSPEIYKA CEKAVGHINP DLDFIRIDKE 250 260 270 280 290 300 EFMSCPSDSI DYAVMEHTQH AVVIPMSAGW SDVGSWSSLW DISNKDHQRN VLKGDIFAHA 310 320 330 340 350 360 CNDNYIYSED MFISAIGVSN LVIVQTTDAL LVANKDTVQD VKKIVDYLKR NDRNEYKQHQ 370 380 390 400 410 420 EVFRPWGKYN VIDSGKNYLV RCITVKPGEK FVAQMHHHRA EHWIVLSGTA RVTKGEQTYM 430 440 450 460 470 VSENESTFIP PNTIHALENP GMTPLKLIEI QSGTYLGEDD IIRLEQRSGF SKEWTNERS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)." Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R. Mol. Microbiol. 5:695-713(1991) [PubMed: 1710759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Expression, purification and characterization of recombinant phosphomannomutase and GDP-alpha-D-mannose pyrophosphorylase from Salmonella enterica, group B, for the synthesis of GDP-alpha-D-mannose from D-mannose." Elling L., Ritter J.E., Verseck S. Glycobiology 6:591-597(1996) [PubMed: 8922954] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-13, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: LT2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56793 Genomic DNA. Translation: CAA40128.1. AE008792 Genomic DNA. Translation: AAL20988.1.
PIR	S15312.
RefSeq	NP_461029.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1253605.
GenomeReviews	Gene locus STM2084 in contig AE006468_GR.
KEGG	stm:STM2084.
NMPDR	fig|99287.1.peg.2009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P26404.
OMA	P26404. VRFEDIY.
Enzyme and pathway databases
BioCyc	STYP99287:STM2084-MON.
BRENDA	2.7.7.13. 2.
Family and domain databases
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
ProDom	PD002664. Man6P_isomerII. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFBM_SALTY

Accession

Primary (citable) accession number: P26404

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)