P26397 (RFBG_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDP-glucose 4,6-dehydratase
EC=4.2.1.45

Gene names

Name:	rfbG
Ordered Locus Names:	STM2091

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O.
Cofactor	NAD.
Pathway	Nucleotide-sugar biosynthesis; CDP-3,6-dideoxy-D-mannose biosynthesis; CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate: step 2/5. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	NAD
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	CDP-glucose 4,6-dehydratase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 359

359

CDP-glucose 4,6-dehydratase

PRO_0000097294

Secondary structure

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359

Helix Strand Turn

Details...

Helix

4 – 7

4

Beta strand

11 – 15

5

Turn

16 – 18

3

Helix

20 – 31

12

Beta strand

35 – 42

8

Beta strand

44 – 47

4

Helix

49 – 52

4

Helix

55 – 57

3

Beta strand

59 – 63

5

Helix

69 – 79

11

Beta strand

82 – 86

5

Helix

93 – 98

6

Helix

100 – 121

22

Beta strand

126 – 130

5

Helix

133 – 135

3

Beta strand

141 – 143

3

Helix

156 – 175

20

Helix

178 – 180

3

Helix

181 – 184

4

Beta strand

187 – 193

7

Beta strand

195 – 197

3

Helix

207 – 216

10

Beta strand

221 – 224

4

Beta strand

232 – 234

3

Helix

236 – 251

16

Helix

253 – 256

4

Beta strand

258 – 262

5

Helix

266 – 268

3

Helix

272 – 283

12

Beta strand

289 – 291

3

Helix

308 – 313

6

Helix

322 – 337

16

Helix

342 – 355

14

Sequences

	Sequence		Length	Mass (Da)
	P26397 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: D6EB7B3D36B2F9FB	FASTA	359	41,022
	10 20 30 40 50 60 MIDKNFWQGK RVFVTGHTGF KGSWLSLWLT EMGAIVKGYA LDAPTVPSLF EIVRLNDLME 70 80 90 100 110 120 SHIGDIRDFE KLRNSIAEFK PEIVFHMAAQ PLVRLSYEQP IETYSTNVMG TVHLLETVKQ 130 140 150 160 170 180 VGNIKAVVNI TSDKCYDNRE WVWGYRENEP MGGYDPYSNS KGCAELVASA FRNSFFNPAN 190 200 210 220 230 240 YEQHGVGLAS VRAGNVIGGG DWAKDRLIPD ILRSFENNQQ VIIRNPYSIR PWQHVLEPLS 250 260 270 280 290 300 GYIVVAQRLY TEGAKFSEGW NFGPRDEDAK TVEFIVDKMV TLWGDDASWL LDGENHPHEA 310 320 330 340 350 HYLKLDCSKA NMQLGWHPRW GLTETLGRIV KWHKAWIRGE DMLICSKREI SDYMSATTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)." Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R. Mol. Microbiol. 5:695-713(1991) [PubMed: 1710759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56793 Genomic DNA. Translation: CAA40121.1.
AE006468 Genomic DNA. Translation: AAL20995.1.

PIR

S15305.

RefSeq

NP_461036.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WVG	X-ray	1.80	A/B	2-359	[»]

ProteinModelPortal

P26397.

SMR

P26397. Positions 2-356.

ModBase

Search...

Proteomic databases

PRIDE

P26397.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1253612.

GenomeReviews

Gene locus STM2091 in contig AE006468_GR.

KEGG

stm:STM2091.

NMPDR

fig|99287.1.peg.2016.

PATRIC

32382765. VBISalEnt20916_2213.

Phylogenomic databases

HOGENOM

HBG755066.

OMA

CYENREW.

ProtClustDB

CLSK894271.

Enzyme and pathway databases

BioCyc

STYP99287:STM2091-MONOMER.

Family and domain databases

InterPro

IPR013445. CDP_4_6_deHydtase.
IPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K01709.

PANTHER

PTHR10366:SF33. CDP_4_6_dhtase. 1 hit.

Pfam

PF01370. Epimerase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02622. CDP_4_6_dhtase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RFBG_SALTY

Accession

Primary (citable) accession number: P26397

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program