P26396 (RFBF_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose-1-phosphate cytidylyltransferase
EC=2.7.7.33

Alternative name(s):
CDP-glucose pyrophosphorylase

Gene names

Name:	rfbF
Ordered Locus Names:	STM2092

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the biosynthesis of the tyvelose, a 3,6-dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate By similarity.
Catalytic activity	CTP + alpha-D-glucose 1-phosphate = diphosphate + CDP-glucose.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; CDP-3,6-dideoxy-D-mannose biosynthesis; CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate: step 1/5. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Subunit structure	Homoexamer By similarity.
Miscellaneous	Catalysis proceeds via a sequential rather than a ping-pong bi-bi reaction mechanism By similarity.
Sequence similarities	Belongs to the glucose-1-phosphate cytidylyltransferase family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-1-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Glucose-1-phosphate cytidylyltransferase		PRO_0000097293
Regions
	Region	6 – 10	5	Substrate binding By similarity
	Region	11 – 13	3	Substrate binding By similarity
Sites
	Metal binding	129	1	Magnesium By similarity
	Metal binding	234	1	Magnesium By similarity
	Binding site	23	1	Substrate By similarity
	Binding site	104	1	Substrate; via carbonyl oxygen By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	128	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26396 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 04DB33D51357D70E	FASTA	257	29,035
	10 20 30 40 50 60 MKAVILAGGL GTRLSEETIV KPKPMVEIGG KPILWHIMKM YSVHGIKDFI ICCGYKGYVI 70 80 90 100 110 120 KEYFANYFLH MSDVTFHMAE NRMEVHHKRV EPWNVTLVDT GDSSMTGGRL KRVAEYVKDD 130 140 150 160 170 180 EAFLFTYGDG VADLDIKATI DFHKAHGKKA TLTATFPPGR FGALDIRAGQ VRSFQEKPKG 190 200 210 220 230 240 DGAMINGGFF VLNPSVIDLI DNDATTWEQE PLMTLAQQGE LMAFEHPGFW QPMDTLRDKV 250 YLEGLWEKGK APWKTWE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)." Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R. Mol. Microbiol. 5:695-713(1991) [PubMed: 1710759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56793 Genomic DNA. Translation: CAA40120.1. AE006468 Genomic DNA. Translation: AAL20996.1.
PIR	S15304.
RefSeq	NP_461037.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P26396.
SMR	P26396. Positions 2-257.
ModBase	Search...
Proteomic databases
PRIDE	P26396.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1253613.
GenomeReviews	Gene locus STM2092 in contig AE006468_GR.
KEGG	stm:STM2092.
NMPDR	fig|99287.1.peg.2017.
PATRIC	32382767. VBISalEnt20916_2214.
Phylogenomic databases
HOGENOM	HBG688195.
OMA	LKPKPMI.
ProtClustDB	CLSK889419.
Enzyme and pathway databases
BioCyc	STYP99287:STM2092-MONOMER.
Family and domain databases
InterPro	IPR013446. G1P_cyt_trans. IPR005835. NTP_transferase. [Graphical view]
KO	K00978.
PANTHER	PTHR22572:SF16. G1P_cyt_trans. 1 hit.
Pfam	PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02623. G1P_cyt_trans. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFBF_SALTY

Accession

Primary (citable) accession number: P26396

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program