Reviewed, UniProtKB/Swiss-Prot P26396 (RFBF_SALTY)

Names and origin

Protein names

Recommended name:
    Glucose-1-phosphate cytidylyltransferase
    EC=2.7.7.33
Alternative name(s):
    CDP-glucose pyrophosphorylase

Gene names

Name:	rfbF
Ordered Locus Names:	STM2092

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	CTP + alpha-D-glucose 1-phosphate = diphosphate + CDP-glucose.
Pathway	Nucleotide-sugar biosynthesis; CDP-3,6-dideoxy-D-mannose biosynthesis; CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate: step 1/5. Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose-1-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 257

257

Glucose-1-phosphate cytidylyltransferase

PRO_0000097293

Secondary structure

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257

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

23 – 25

3

Helix

33 – 43

11

Beta strand

48 – 53

6

Helix

57 – 65

9

Helix

67 – 71

5

Beta strand

74 – 77

4

Helix

78 – 80

3

Beta strand

82 – 85

4

Beta strand

94 – 99

6

Helix

106 – 112

7

Helix

113 – 116

4

Turn

117 – 119

3

Beta strand

123 – 127

5

Beta strand

130 – 132

3

Helix

136 – 146

11

Beta strand

149 – 155

7

Beta strand

161 – 167

7

Beta strand

170 – 177

8

Beta strand

190 – 192

3

Helix

194 – 199

6

Turn

207 – 209

3

Helix

210 – 217

8

Beta strand

221 – 226

6

Beta strand

230 – 232

3

Helix

236 – 247

12

Sequences

	Sequence		Length	Mass (Da)
	P26396-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 04DB33D51357D70E	FASTA	257	29,035
	10 20 30 40 50 60 MKAVILAGGL GTRLSEETIV KPKPMVEIGG KPILWHIMKM YSVHGIKDFI ICCGYKGYVI 70 80 90 100 110 120 KEYFANYFLH MSDVTFHMAE NRMEVHHKRV EPWNVTLVDT GDSSMTGGRL KRVAEYVKDD 130 140 150 160 170 180 EAFLFTYGDG VADLDIKATI DFHKAHGKKA TLTATFPPGR FGALDIRAGQ VRSFQEKPKG 190 200 210 220 230 240 DGAMINGGFF VLNPSVIDLI DNDATTWEQE PLMTLAQQGE LMAFEHPGFW QPMDTLRDKV 250 YLEGLWEKGK APWKTWE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)." Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R. Mol. Microbiol. 5:695-713(1991) [PubMed: 1710759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X56793 Genomic DNA. Translation: CAA40120.1.
AE008792 Genomic DNA. Translation: AAL20996.1.

PIR

S15304.

RefSeq

NP_461037.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TZF	X-ray	2.10	A	2-257	[»]
1WVC	X-ray	2.50	A	2-257	[»]

ModBase

Search...

Genome annotation databases

GeneID

1253613.

GenomeReviews

Gene locus STM2092 in contig AE006468_GR.

KEGG

stm:STM2092.

NMPDR

fig|99287.1.peg.2017.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P26396.

OMA

P26396. KPKPMIE.

Enzyme and pathway databases

BioCyc

STYP99287:STM2092-MON.

BRENDA

2.7.7.33. 2.

Family and domain databases

InterPro

IPR013446. G1P_cyt_trans.
IPR005835. NTP_transferase.
[Graphical view]

PANTHER

PTHR22572:SF16. G1P_cyt_trans. 1 hit.

Pfam

PF00483. NTP_transferase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02623. G1P_cyt_trans. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RFBF_SALTY

Accession

Primary (citable) accession number: P26396

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)