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Protein

Glucose-1-phosphate cytidylyltransferase

Gene

rfbF

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the tyvelose, a 3,6-dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate (By similarity).By similarity

Catalytic activityi

CTP + alpha-D-glucose 1-phosphate = diphosphate + CDP-glucose.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: CDP-3,6-dideoxy-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glucose-1-phosphate cytidylyltransferase (rfbF)
  2. CDP-glucose 4,6-dehydratase (rfbG)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway CDP-3,6-dideoxy-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate, the pathway CDP-3,6-dideoxy-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231SubstrateBy similarity
Binding sitei104 – 1041Substrate; via carbonyl oxygenBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei128 – 1281Substrate; via amide nitrogenBy similarity
Metal bindingi129 – 1291MagnesiumBy similarity
Metal bindingi234 – 2341MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-2104-MONOMER.
UniPathwayiUPA00055; UER00511.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate cytidylyltransferase (EC:2.7.7.33)
Alternative name(s):
CDP-glucose pyrophosphorylase
Gene namesi
Name:rfbF
Ordered Locus Names:STM2092
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Glucose-1-phosphate cytidylyltransferasePRO_0000097293Add
BLAST

Proteomic databases

PaxDbiP26396.
PRIDEiP26396.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi99287.STM2092.

Structurei

3D structure databases

ProteinModelPortaliP26396.
SMRiP26396. Positions 2-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni6 – 105Substrate bindingBy similarity
Regioni11 – 133Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EJZ. Bacteria.
COG1208. LUCA.
HOGENOMiHOG000283474.
KOiK00978.
OMAiYSEPWRV.
OrthoDBiEOG632D11.
PhylomeDBiP26396.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR013446. G1P_cyt_trans.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF16. PTHR22572:SF16. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02623. G1P_cyt_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

P26396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVILAGGL GTRLSEETIV KPKPMVEIGG KPILWHIMKM YSVHGIKDFI
60 70 80 90 100
ICCGYKGYVI KEYFANYFLH MSDVTFHMAE NRMEVHHKRV EPWNVTLVDT
110 120 130 140 150
GDSSMTGGRL KRVAEYVKDD EAFLFTYGDG VADLDIKATI DFHKAHGKKA
160 170 180 190 200
TLTATFPPGR FGALDIRAGQ VRSFQEKPKG DGAMINGGFF VLNPSVIDLI
210 220 230 240 250
DNDATTWEQE PLMTLAQQGE LMAFEHPGFW QPMDTLRDKV YLEGLWEKGK

APWKTWE
Length:257
Mass (Da):29,035
Last modified:August 1, 1992 - v1
Checksum:i04DB33D51357D70E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56793 Genomic DNA. Translation: CAA40120.1.
AE006468 Genomic DNA. Translation: AAL20996.1.
PIRiS15304.
RefSeqiNP_461037.1. NC_003197.1.
WP_000648784.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20996; AAL20996; STM2092.
GeneIDi1253613.
KEGGistm:STM2092.
PATRICi32382767. VBISalEnt20916_2214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56793 Genomic DNA. Translation: CAA40120.1.
AE006468 Genomic DNA. Translation: AAL20996.1.
PIRiS15304.
RefSeqiNP_461037.1. NC_003197.1.
WP_000648784.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP26396.
SMRiP26396. Positions 2-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2092.

Proteomic databases

PaxDbiP26396.
PRIDEiP26396.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20996; AAL20996; STM2092.
GeneIDi1253613.
KEGGistm:STM2092.
PATRICi32382767. VBISalEnt20916_2214.

Phylogenomic databases

eggNOGiENOG4105EJZ. Bacteria.
COG1208. LUCA.
HOGENOMiHOG000283474.
KOiK00978.
OMAiYSEPWRV.
OrthoDBiEOG632D11.
PhylomeDBiP26396.

Enzyme and pathway databases

UniPathwayiUPA00055; UER00511.
UPA00281.
BioCyciSENT99287:GCTI-2104-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR013446. G1P_cyt_trans.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF16. PTHR22572:SF16. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR02623. G1P_cyt_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and sequence of the rfb (O antigen) gene cluster of Salmonella serovar typhimurium (strain LT2)."
    Jiang X.-M., Neal B., Santiago F., Lee S.J., Romana L.K., Reeves P.R.
    Mol. Microbiol. 5:695-713(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiRFBF_SALTY
AccessioniPrimary (citable) accession number: P26396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 11, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Catalysis proceeds via a sequential rather than a ping-pong bi-bi reaction mechanism.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.