Fructose-specific phosphotransferase enzyme IIB component

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Reviewed, UniProtKB/Swiss-Prot P26380 (PTFB_BACSU)

Last modified February 9, 2010. Version 82. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Fructose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
Alternative name(s):
    PTS system fructose-specific EIIB component
    EIIB-Fru
    p18

Gene names

Name:	levE
Synonyms:	sacL
Ordered Locus Names:	BSU27060

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport. LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-4 domain.

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Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 163

163

Fructose-specific phosphotransferase enzyme IIB component

PRO_0000186524

Regions

Domain

1 – 163

163

PTS EIIB type-4

Sites

Active site

Pros-phosphohistidine intermediate By similarity

Secondary structure

163

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P26380-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: F3C10058A8333177
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FASTA

163

18,215

        10         20         30         40         50         60 
MMNIVLARID DRFIHGQILT RWIKVHAADR IIVVSDDIAQ DEMRKTLILS VAPSNVKASA 

        70         80         90        100        110        120 
VSVSKMAKAF HSPRYEGVTA MLLFENPSDI VSLIEAGVPI KTVNVGGMRF ENHRRQITKS 

       130        140        150        160 
VSVTEQDIKA FETLSDKGVK LELRQLPSDA SEDFVQILRN VTK

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon." Martin-Verstraete I., Debarbouille M., Klier A., Rapoport G. J. Mol. Biol. 214:657-671(1990) [PubMed: 2117666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"A 23911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the lev operon." Parro V., San Roman M., Galindo I., Purnelle B., Bolotin A., Sorokin A., Mellado R.P. Microbiology 143:1321-1326(1997) [PubMed: 9141695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Crystal structure of the IIB subunit of a fructose permease (IIBLev) from Bacillus subtilis." Schauder S., Nunn R.S., Lanz R., Erni B., Schirmer T. J. Mol. Biol. 276:591-602(1998) [PubMed: 9551099] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56098 Genomic DNA. Translation: CAA39578.1.
X92868 Genomic DNA. Translation: CAA63462.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB14648.1. Different initiation.

PIR

S11399.

RefSeq

NP_390584.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BLE	X-ray	2.90	A	1-163	[»]

ModBase

Search...

Protein family/group databases

TCDB

4.A.6.1.2. PTS mannose-fructose-sorbose (Man) family.

Genome annotation databases

GeneID

937078.

GenomeReviews

Gene locus BSU27060 in contig AL009126_GR.

KEGG

bsu:BSU27060.

NMPDR

fig|224308.1.peg.2709.

Organism-specific databases

SubtiList

BG10317. levE. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

HBG702768.

PhylomeDB

P26380.

Enzyme and pathway databases

BRENDA

2.7.1.69. 150.

Family and domain databases

InterPro

IPR004720. PTS_IIB_sorbose-sp.
IPR018455. PTS_IIB_sorbose-sp_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.35.10. PTS_IIB_sorb. 1 hit.

Pfam

PF03830. PTSIIB_sorb. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00854. pts-sorbose. 1 hit.

PROSITE

PS51101. PTS_EIIB_TYPE_4. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

PTFB_BACSU

Accession

Primary (citable) accession number: P26380

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families