Reviewed, UniProtKB/Swiss-Prot P26366 (AMIB_SALTY)

Names and origin

Protein names

Recommended name:
    N-acetylmuramoyl-L-alanine amidase amiB
    EC=3.5.1.28

Gene names

Name:	amiB
Ordered Locus Names:	STM4358

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell-wall hydrolase probably involved in cell-wall hydrolysis, septation or recycling.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 439	417	N-acetylmuramoyl-L-alanine amidase amiB		PRO_0000006463
Regions
	Compositional bias	130 – 135	6	Poly-Pro

Sequences

	Sequence		Length	Mass (Da)
	P26366-1 [UniParc]. Last modified December 19, 2001. Version 2. Checksum: 01B2BEB753D34DE9	FASTA	439	46,790
	10 20 30 40 50 60 MIYRIKNAVI AALILLCAQA GAASLSDIQV SNGEQQARIT LSFIGEPEYA YSQDGKRTVA 70 80 90 100 110 120 LDIRQTGVIQ GLPLQFSGNN LVKTIRAGTP KDAQSLRLLV DLTENGKTEA VKRQNGGNYT 130 140 150 160 170 180 VIFTINADVP PPPPPVVAKR VESAPRPTEP ARNPFKSSDD RLTGVTSSNT VTRPAARASA 190 200 210 220 230 240 GAGDKVVIAI DAGHGGQDPG AIGPGGTREK NVTIAIARKL RTLLNNDPMF KGVLTRDGDY 250 260 270 280 290 300 FISVMGRSDV ARKQNANFLV SIHADAAPNR DATGASVWVL SNRRANSEMA NWLEQHEKQS 310 320 330 340 350 360 ELLGGAGDVL ANSQSDPYLS QAVLDLQFGH SQRVGYDVAT NVLSQLDGVG SLHKRRPEHA 370 380 390 400 410 420 SLGVLRSPDI PSILVETGFI SNHGEERLLA SDRYQQQIAD AIYRGLRKYF AAHPIQSAPQ 430 GGPGQTASTN QPGAITAAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[2]	"Nucleotide sequence of the Salmonella typhimurium mutL gene required for mismatch repair: homology of MutL to HexB of Streptococcus pneumoniae and to PMS1 of the yeast Saccharomyces cerevisiae." Mankovich J.A., McIntyre C.A., Walker G.C. J. Bacteriol. 171:5325-5331(1989) [PubMed: 2676972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-439. Strain: LT2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006468 Genomic DNA. Translation: AAL23178.1. M29687 Genomic DNA. No translation available.
RefSeq	NP_463219.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P26366.
Genome annotation databases
GeneID	1255884.
GenomeReviews	Gene locus STM4358 in contig AE006468_GR.
KEGG	stm:STM4358.
NMPDR	fig|99287.1.peg.4192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P26366.
OMA	ANSQADP.
Enzyme and pathway databases
BioCyc	STYP99287:STM4358-MON.
BRENDA	3.5.1.28. 2.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIB_SALTY

Accession

Primary (citable) accession number: P26366

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	December 19, 2001
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)