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Protein

GTP:AMP phosphotransferase, mitochondrial

Gene

ADK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities. Does not accept ATP as phosphate donor.UniRule annotation2 Publications

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius. Active at 30 degrees Celsius, and rapidly inactivated with an increase of 5 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461AMPUniRule annotation
Binding sitei51 – 511AMPUniRule annotation
Binding sitei110 – 1101AMPUniRule annotation
Binding sitei145 – 1451GTPUniRule annotation
Binding sitei178 – 1781AMPUniRule annotation
Binding sitei189 – 1891AMPUniRule annotation
Binding sitei218 – 2181GTP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 296GTPUniRule annotation
Nucleotide bindingi72 – 743AMPUniRule annotation
Nucleotide bindingi103 – 1064AMPUniRule annotation
Nucleotide bindingi154 – 1552GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER170W-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-SCE-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP:AMP phosphotransferase, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation)
Alternative name(s):
Adenylate kinase 3UniRule annotation
Short name:
AK 3UniRule annotation
Gene namesi
Name:ADK2UniRule annotation
Synonyms:AKY3, PAK3
Ordered Locus Names:YER170W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER170W.
SGDiS000000972. ADK2.

Subcellular locationi

  • Mitochondrion matrix UniRule annotation1 Publication

GO - Cellular componenti

  • mitochondrial inner membrane Source: SGD
  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225GTP:AMP phosphotransferase, mitochondrialPRO_0000158907Add
BLAST

Expressioni

Inductioni

Expression level is low on glucose or other fermentable carbon sources. Induced about 3-fold on glycerol, and significantly induced by ethanol.1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

BioGridi36923. 16 interactions.
DIPiDIP-4877N.
IntActiP26364. 1 interaction.
MINTiMINT-517522.

Structurei

3D structure databases

ProteinModelPortaliP26364.
SMRiP26364. Positions 15-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni45 – 7430NMPbindUniRule annotationAdd
BLAST
Regioni144 – 18138LIDUniRule annotationAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
InParanoidiP26364.
KOiK00939.
OMAiIAGRWVH.
OrthoDBiEOG7BCNPJ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03169. Adenylate_kinase_AK3.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKADAKQITH LLKPLRLLLL GAPGSGKGTQ TSRLLKQIPQ LSSISSGDIL
60 70 80 90 100
RQEIKSESTL GREATTYIAQ GKLLPDDLIT RLITFRLSAL GWLKPSAMWL
110 120 130 140 150
LDGFPRTTAQ ASALDELLKQ HDASLNLVVE LDVPESTILE RIENRYVHVP
160 170 180 190 200
SGRVYNLQYN PPKVPGLDDI TGEPLTKRLD DTAEVFKKRL EEYKKTNEPL
210 220
KDYYKKSGIF GTVSGETSDI IFRNY
Length:225
Mass (Da):25,194
Last modified:August 1, 1992 - v1
Checksum:i3B192BE2535BF91F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77757 Genomic DNA. Translation: AAA34418.1.
X65126 Genomic DNA. Translation: CAA46254.1.
U18922 Genomic DNA. Translation: AAB64697.1.
AY558457 Genomic DNA. Translation: AAS56783.1.
BK006939 Genomic DNA. Translation: DAA07832.1.
PIRiS23568.
RefSeqiNP_011097.3. NM_001179060.3.

Genome annotation databases

EnsemblFungiiYER170W; YER170W; YER170W.
GeneIDi856917.
KEGGisce:YER170W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77757 Genomic DNA. Translation: AAA34418.1.
X65126 Genomic DNA. Translation: CAA46254.1.
U18922 Genomic DNA. Translation: AAB64697.1.
AY558457 Genomic DNA. Translation: AAS56783.1.
BK006939 Genomic DNA. Translation: DAA07832.1.
PIRiS23568.
RefSeqiNP_011097.3. NM_001179060.3.

3D structure databases

ProteinModelPortaliP26364.
SMRiP26364. Positions 15-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36923. 16 interactions.
DIPiDIP-4877N.
IntActiP26364. 1 interaction.
MINTiMINT-517522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER170W; YER170W; YER170W.
GeneIDi856917.
KEGGisce:YER170W.

Organism-specific databases

EuPathDBiFungiDB:YER170W.
SGDiS000000972. ADK2.

Phylogenomic databases

GeneTreeiENSGT00550000074679.
HOGENOMiHOG000238772.
InParanoidiP26364.
KOiK00939.
OMAiIAGRWVH.
OrthoDBiEOG7BCNPJ.

Enzyme and pathway databases

BioCyciYEAST:YER170W-MONOMER.
ReactomeiR-SCE-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-SCE-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

NextBioi983371.
PROiP26364.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03169. Adenylate_kinase_AK3.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A putative second adenylate kinase-encoding gene from the yeast Saccharomyces cerevisiae."
    Cooper A.J., Friedberg E.C.
    Gene 114:145-148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A new member of the adenylate kinase family in yeast: PAK3 is highly homologous to mammalian AK3 and is targeted to mitochondria."
    Schricker R., Magdolen V., Bandlow W.
    Mol. Gen. Genet. 233:363-371(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, INDUCTION.
    Strain: DL-1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Strain-dependent occurrence of functional GTP:AMP phosphotransferase (AK3) in Saccharomyces cerevisiae."
    Schricker R., Magdolen V., Strobel G., Bogengruber E., Breitenbach M., Bandlow W.
    J. Biol. Chem. 270:31103-31110(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: DL-1.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A GTP:AMP phosphotransferase, Adk2p, in Saccharomyces cerevisiae. Role of the C terminus in protein folding/stabilization, thermal tolerance, and enzymatic activity."
    Gu Y., Gordon D.M., Amutha B., Pain D.
    J. Biol. Chem. 280:18604-18609(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiKAD3_YEAST
AccessioniPrimary (citable) accession number: P26364
Secondary accession number(s): D3DM78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 11, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication
Depending on the yeast strain, the GTP:AMP phosphotransferase is encoded by ADK2 with or without a single base pair deletion/insertion near the 3' end of the open reading frame, resulting in a long or a short form. The ADK2 short form (this entry) is also referred to as PAK3, while the long form has been named AKY3. A sequence of the long form can be found in strain D273-10B (AC E9P974). The modified C-terminus in the long form contributes to protein folding and is critical for protein stability.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.