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Protein

DNA mismatch repair protein msh3

Gene

msh3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with msh2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. Msh3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions (By similarity). Involved in termination of copy-synthesis during mating-type switching.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi759 – 7668ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • gene conversion at mating-type locus Source: PomBase
  • gene conversion at mating-type locus, termination of copy-synthesis Source: PomBase
  • maintenance of DNA repeat elements Source: PomBase
  • reciprocal meiotic recombination Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein msh3
Alternative name(s):
Mating-type switching protein swi4
MutS protein homolog 3
Gene namesi
Name:msh3
Synonyms:swi4
ORF Names:SPAC8F11.03
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

PomBaseiSPAC8F11.03. msh3.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: PomBase
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 993993DNA mismatch repair protein msh3PRO_0000115196Add
BLAST

Proteomic databases

PRIDEiP26359.

Interactioni

Subunit structurei

Heterodimer consisting of msh2-msh3 (MutS beta). Forms a ternary complex with MutL alpha (mlh1-pms1) (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4687921.

Structurei

3D structure databases

ProteinModelPortaliP26359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 211115Mispair-binding domainBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

InParanoidiP26359.
OrthoDBiEOG773XQH.
PhylomeDBiP26359.

Family and domain databases

Gene3Di3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGMSYNITH ECDAINILSD NLHEGAISED MVALSGPAIE LLENNVGSSK
60 70 80 90 100
NSYQEDEGSS SIDENAPLIS IKRKRRIRTV KSTSNKELVQ RKASKPTKQK
110 120 130 140 150
SVFTPLEQQY LELKKNYQET ILAIEVGYKF RFFGKDAKIA SEVLGISCYF
160 170 180 190 200
EHNFLNASVP SYRIDYHLER LINFGLKVAV VRQTETAALK STSSSRNTLF
210 220 230 240 250
DRRVARVLTK GTTLDDSFFR FEQTQHGTLQ ASQFILCVAD NVDKSKAKSG
260 270 280 290 300
RVQVGLIAIQ LSSGTTVYDH FQDDFLRSEL QTRLSHFQPC ELIYSNKLSS
310 320 330 340 350
ESVALLNHYV STEKTCGRVV RVQHAVQQDV KLALENLQDF FSSKCIMSGS
360 370 380 390 400
KIIELHMEKV KSLHSLSIIC LDMAISYLME FSLEDLFVAS NFYQPFDSIS
410 420 430 440 450
SMVLSKQALE GLELFVNQTD HTPVGSLFWV LDRTYTRFGQ RMLQRWLQKP
460 470 480 490 500
LVDKENIIER LDAVEELAFN SNSQVQAIRK MLYRLPDLEK GLSRIYYQRG
510 520 530 540 550
FYKAASAFSK NSYSCFKSAL LRRLIQQLPS ISSIIDHFLG MFDQKEAENN
560 570 580 590 600
NKVDMFKDID NFDLSEEPND VDYELAQEIR ELKMSILMVR TEMDFHLQEL
610 620 630 640 650
RDYLEYPNLE FSIWGNVKFC IEVSKGCKKI PPDWIKLSST RSLFRFHTPK
660 670 680 690 700
IQSLLIELSS HEENLTISSE KIYRSFLSRI SEHYNELRNV TTVLGTLDCL
710 720 730 740 750
ISFARISSQS GYTRPEFSDK ELLIHESRHP MIELLSDKSF VPNHIHLSSD
760 770 780 790 800
GVRCLLITGP NMGGKSSFVK QLALSAIMAQ SGCFVPAKSA LLPIFDSILI
810 820 830 840 850
RMGSSDNLSV NMSTFMVEML ETKEVLSKAT EKSMVIIDEL GRGTSTIDGE
860 870 880 890 900
AISYAVLHYL NQYIKSYLLF VTHFPSLGIL ERRFEGQLRC FHMGYLKSKE
910 920 930 940 950
DFETSVSQSI SFLYKLVPGV ASKSYGLNVA RMAGIPFSIL SRATEISENY
960 970 980 990
EKKHRNARKN VFIRKVAKLL MILNAEEIDF KRLFYDLTAF EEI
Length:993
Mass (Da):113,362
Last modified:May 1, 1992 - v1
Checksum:i44B80E553A59B708
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti499 – 4991R → RCTPSEMFHVLK in CAB52164 (PubMed:11859360).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61306 Genomic DNA. Translation: CAA43603.1.
CU329670 Genomic DNA. Translation: CAB52164.2.
PIRiS22569. S21964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61306 Genomic DNA. Translation: CAA43603.1.
CU329670 Genomic DNA. Translation: CAB52164.2.
PIRiS22569. S21964.

3D structure databases

ProteinModelPortaliP26359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4687921.

Proteomic databases

PRIDEiP26359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

PomBaseiSPAC8F11.03. msh3.

Phylogenomic databases

InParanoidiP26359.
OrthoDBiEOG773XQH.
PhylomeDBiP26359.

Enzyme and pathway databases

ReactomeiR-SPO-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Miscellaneous databases

PROiP26359.

Family and domain databases

Gene3Di3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes."
    Fleck O., Michael H., Heim L.
    Nucleic Acids Res. 20:2271-2278(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe."
    Tornier C., Bessone S., Varlet I., Rudolph C., Darmon M., Fleck O.
    Genetics 158:65-75(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Control of GT repeat stability in Schizosaccharomyces pombe by mismatch repair factors."
    Mansour A.A., Tornier C., Lehmann E., Darmon M., Fleck O.
    Genetics 158:77-85(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSH3_SCHPO
AccessioniPrimary (citable) accession number: P26359
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.