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Protein

Nitrogen regulatory protein DAL80

Gene

DAL80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Negative regulator of multiple nitrogen catabolic genes including the allantoin pathway genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri31 – 5525GATA-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: SGD
  • sequence-specific DNA binding RNA polymerase II transcription factor activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nitrate assimilation Source: UniProtKB-KW
  • nitrogen catabolite repression of transcription Source: SGD
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein DAL80
Alternative name(s):
Regulatory protein UGA43
Gene namesi
Name:DAL80
Synonyms:UGA43
Ordered Locus Names:YKR034W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKR034w.
EuPathDBiFungiDB:YKR034W.
SGDiS000001742. DAL80.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Nitrogen regulatory protein DAL80PRO_0000083470Add
BLAST

Expressioni

Inductioni

Sensitive to nitrogen catabolite repression.

Gene expression databases

GenevestigatoriP26343.

Interactioni

Protein-protein interaction databases

BioGridi34165. 57 interactions.
DIPiDIP-2094N.
IntActiP26343. 19 interactions.
MINTiMINT-483456.
STRINGi4932.YKR034W.

Structurei

3D structure databases

ProteinModelPortaliP26343.
SMRiP26343. Positions 30-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi79 – 10022Asn-richAdd
BLAST
Compositional biasi101 – 1088Arg/Lys-rich (basic)

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri31 – 5525GATA-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00760000119221.
InParanoidiP26343.
KOiK09184.
OrthoDBiEOG7TQVCN.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSDSLKLP SPTLSAAAGV DDCDGEDHPT CQNCFTVKTP LWRRDEHGTV
60 70 80 90 100
LCNACGLFLK LHGEPRPISL KTDTIKSRNR KKLNNNNVNT NANTHSNDPN
110 120 130 140 150
KIFKRKKRLL TTGGGSLPTN NPKVSILEKF MVSGSIKPLL KPKETVPNTK
160 170 180 190 200
ECSTQRGKFS LDPCEPSGKN YLYQINGSDI YTSNIELTRL PNLSTLLEPS
210 220 230 240 250
PFSDSAVPEI ELTWKLHNEE EVIKLKTKIS ELELVTDLYK KHIFQLNEKC
260
KQLEVELHSR ASVQSHPQH
Length:269
Mass (Da):30,166
Last modified:May 1, 1992 - v1
Checksum:iF37BEB10038599EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61S → L in CAA42757 (PubMed:1525858).Curated
Sequence conflicti207 – 2071V → I in CAA42757 (PubMed:1525858).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77821 Genomic DNA. Translation: AAA34556.1.
X60199 Genomic DNA. Translation: CAA42757.1.
Z28259 Genomic DNA. Translation: CAA82107.1.
Z28258 Genomic DNA. Translation: CAA82106.1.
BK006944 Genomic DNA. Translation: DAA09187.1.
PIRiS22781.
RefSeqiNP_012959.1. NM_001179824.1.

Genome annotation databases

EnsemblFungiiYKR034W; YKR034W; YKR034W.
GeneIDi853904.
KEGGisce:YKR034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77821 Genomic DNA. Translation: AAA34556.1.
X60199 Genomic DNA. Translation: CAA42757.1.
Z28259 Genomic DNA. Translation: CAA82107.1.
Z28258 Genomic DNA. Translation: CAA82106.1.
BK006944 Genomic DNA. Translation: DAA09187.1.
PIRiS22781.
RefSeqiNP_012959.1. NM_001179824.1.

3D structure databases

ProteinModelPortaliP26343.
SMRiP26343. Positions 30-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34165. 57 interactions.
DIPiDIP-2094N.
IntActiP26343. 19 interactions.
MINTiMINT-483456.
STRINGi4932.YKR034W.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR034W; YKR034W; YKR034W.
GeneIDi853904.
KEGGisce:YKR034W.

Organism-specific databases

CYGDiYKR034w.
EuPathDBiFungiDB:YKR034W.
SGDiS000001742. DAL80.

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00760000119221.
InParanoidiP26343.
KOiK09184.
OrthoDBiEOG7TQVCN.

Enzyme and pathway databases

BioCyciYEAST:G3O-32007-MONOMER.

Miscellaneous databases

NextBioi975233.
PROiP26343.

Gene expression databases

GenevestigatoriP26343.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
[Graphical view]
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the DAL80 gene, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression."
    Cunningham T.S., Cooper T.G.
    Mol. Cell. Biol. 11:6205-6215(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Cunningham T.S., Cooper T.G.
    Mol. Cell. Biol. 12:2454-2454(1992) [PubMed] [Europe PMC] [Abstract]
  3. "The UGA43 negative regulatory gene of Saccharomyces cerevisiae contains both a GATA-1 type zinc finger and a putative leucine zipper."
    Coornaert D., Vissers S., Andre B., Grenson M.
    Curr. Genet. 21:301-307(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiDAL80_YEAST
AccessioniPrimary (citable) accession number: P26343
Secondary accession number(s): D6VX97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 29, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.