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P26339

- CMGA_MOUSE

UniProt

P26339 - CMGA_MOUSE

Protein

Chromogranin-A

Gene

Chga

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromogranin-A
    Short name:
    CgA
    Cleaved into the following 3 chains:
    Gene namesi
    Name:Chga
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:88394. Chga.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle 1 Publication. Cytoplasmic vesiclesecretory vesicle membrane 1 Publication. Secreted 1 Publication
    Note: Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. secretory granule Source: InterPro
    3. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Chaini19 – 463445Chromogranin-APRO_0000005422Add
    BLAST
    Peptidei19 – 151133Beta-graninBy similarityPRO_0000005423Add
    BLAST
    Peptidei276 – 32954PancreastatinBy similarityPRO_0000005424Add
    BLAST
    Peptidei358 – 37114WE-14By similarityPRO_0000005425Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 56By similarity
    Modified residuei119 – 1191PhosphoserineBy similarity
    Modified residuei220 – 2201PhosphoserineBy similarity
    Modified residuei308 – 3081PhosphoserineBy similarity
    Modified residuei329 – 3291Glycine amideCurated
    Modified residuei350 – 3501PhosphoserineBy similarity
    Modified residuei410 – 4101PhosphoserineBy similarity
    Modified residuei414 – 4141PhosphoserineBy similarity

    Post-translational modificationi

    CgA is O-glycosylated.

    Keywords - PTMi

    Amidation, Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP26339.
    PaxDbiP26339.
    PRIDEiP26339.

    PTM databases

    PhosphoSiteiP26339.

    Expressioni

    Gene expression databases

    BgeeiP26339.
    CleanExiMM_CHGA.
    GenevestigatoriP26339.

    Interactioni

    Subunit structurei

    Interacts with SCG3; this interaction is optimal in conditions mimicking the lumenal milieu of the trans-Golgi network, i.e. pH 5.5 and 10 mM Ca(+2).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SOD1P004415EBI-990900,EBI-990792From a different organism.

    Protein-protein interaction databases

    IntActiP26339. 3 interactions.
    STRINGi10090.ENSMUSP00000021610.

    Structurei

    3D structure databases

    ProteinModelPortaliP26339.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi103 – 11614Poly-GlnAdd
    BLAST
    Compositional biasi236 – 24510Poly-Glu
    Compositional biasi340 – 3478Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG41926.
    GeneTreeiENSGT00730000111266.
    HOGENOMiHOG000111808.
    HOVERGENiHBG001272.
    InParanoidiP26339.
    OMAiVNSPMNK.
    OrthoDBiEOG7V766Z.
    PhylomeDBiP26339.
    TreeFamiTF336596.

    Family and domain databases

    InterProiIPR001819. Chromogranin_AB.
    IPR018054. Chromogranin_CS.
    IPR001990. Granin.
    [Graphical view]
    PANTHERiPTHR10583. PTHR10583. 1 hit.
    PfamiPF01271. Granin. 2 hits.
    [Graphical view]
    PRINTSiPR00659. CHROMOGRANIN.
    PROSITEiPS00422. GRANINS_1. 1 hit.
    PS00423. GRANINS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26339-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSTAVLALL LCAGQVFALP VNSPMTKGDT KVMKCVLEVI SDSLSKPSPM    50
    PVSPECLETL QGDERILSIL RHQNLLKELQ DLALQGAKER AQQPLKQQQP 100
    PKQQQQQQQQ QQQEQQHSSF EDELSEVFEN QSPDAKHRDA AAEVPSRDTM 150
    EKRKDSDKGQ QDGFEATTEG PRPQAFPEPN QESPMMGDSE SPGEDTATNT 200
    QSPTSLPSQE HVDPQATGDS ERGLSAQQQA RKAKQEEKEE EEEEEAVARE 250
    KAGPEEVPTA ASSSHFHAGY KAIQKDDGQS DSQAVDGDGK TEASEALPSE 300
    GKGELEHSQQ EEDGEEAMVG TPQGLFPQGG KGRELEHKQE EEEEEEERLS 350
    REWEDKRWSR MDQLAKELTA EKRLEGEDDP DRSMKLSFRT RAYGFRDPGP 400
    QLRRGWRPSS REDSVEARSD FEEKKEEEGS ANRRAEDQEL ESLSAIEAEL 450
    EKVAHQLQAL RRG 463
    Length:463
    Mass (Da):51,789
    Last modified:May 1, 1992 - v1
    Checksum:i1AB3C5FF433C39E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64278 mRNA. Translation: AAA37457.1.
    BC026554 mRNA. Translation: AAH26554.1.
    CCDSiCCDS26120.1.
    PIRiA39868.
    RefSeqiNP_031719.1. NM_007693.1.
    UniGeneiMm.4137.

    Genome annotation databases

    EnsembliENSMUST00000021610; ENSMUSP00000021610; ENSMUSG00000021194.
    GeneIDi12652.
    KEGGimmu:12652.
    UCSCiuc007oui.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64278 mRNA. Translation: AAA37457.1 .
    BC026554 mRNA. Translation: AAH26554.1 .
    CCDSi CCDS26120.1.
    PIRi A39868.
    RefSeqi NP_031719.1. NM_007693.1.
    UniGenei Mm.4137.

    3D structure databases

    ProteinModelPortali P26339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P26339. 3 interactions.
    STRINGi 10090.ENSMUSP00000021610.

    PTM databases

    PhosphoSitei P26339.

    Proteomic databases

    MaxQBi P26339.
    PaxDbi P26339.
    PRIDEi P26339.

    Protocols and materials databases

    DNASUi 12652.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021610 ; ENSMUSP00000021610 ; ENSMUSG00000021194 .
    GeneIDi 12652.
    KEGGi mmu:12652.
    UCSCi uc007oui.1. mouse.

    Organism-specific databases

    CTDi 1113.
    MGIi MGI:88394. Chga.

    Phylogenomic databases

    eggNOGi NOG41926.
    GeneTreei ENSGT00730000111266.
    HOGENOMi HOG000111808.
    HOVERGENi HBG001272.
    InParanoidi P26339.
    OMAi VNSPMNK.
    OrthoDBi EOG7V766Z.
    PhylomeDBi P26339.
    TreeFami TF336596.

    Miscellaneous databases

    NextBioi 281864.
    PROi P26339.
    SOURCEi Search...

    Gene expression databases

    Bgeei P26339.
    CleanExi MM_CHGA.
    Genevestigatori P26339.

    Family and domain databases

    InterProi IPR001819. Chromogranin_AB.
    IPR018054. Chromogranin_CS.
    IPR001990. Granin.
    [Graphical view ]
    PANTHERi PTHR10583. PTHR10583. 1 hit.
    Pfami PF01271. Granin. 2 hits.
    [Graphical view ]
    PRINTSi PR00659. CHROMOGRANIN.
    PROSITEi PS00422. GRANINS_1. 1 hit.
    PS00423. GRANINS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and function of the chromogranin A gene. Clues to evolution and tissue-specific expression."
      Wu H.J., Rozansky D.J., Parmer R.J., Gill B.M., O'Connor D.T.
      J. Biol. Chem. 266:13130-13134(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    3. "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells."
      Hosaka M., Watanabe T., Sakai Y., Uchiyama Y., Takeuchi T.
      Mol. Biol. Cell 13:3388-3399(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCG3, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCMGA_MOUSE
    AccessioniPrimary (citable) accession number: P26339
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds calcium with a low-affinity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3