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Protein

Chromogranin-A

Gene

Chga

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Chromogranin-A
Short name:
CgA
Cleaved into the following 3 chains:
Gene namesi
Name:Chga
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:88394. Chga.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle 1 Publication. Cytoplasmic vesiclesecretory vesicle membrane 1 Publication. Secreted 1 Publication
Note: Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. perinuclear region of cytoplasm Source: MGI
  3. secretory granule Source: InterPro
  4. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Chaini19 – 463445Chromogranin-APRO_0000005422Add
BLAST
Peptidei19 – 151133Beta-graninBy similarityPRO_0000005423Add
BLAST
Peptidei276 – 32954PancreastatinBy similarityPRO_0000005424Add
BLAST
Peptidei358 – 37114WE-14By similarityPRO_0000005425Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 56By similarity
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei220 – 2201PhosphoserineBy similarity
Modified residuei308 – 3081PhosphoserineBy similarity
Modified residuei329 – 3291Glycine amideCurated
Modified residuei350 – 3501PhosphoserineBy similarity
Modified residuei410 – 4101PhosphoserineBy similarity
Modified residuei414 – 4141PhosphoserineBy similarity

Post-translational modificationi

CgA is O-glycosylated.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP26339.
PaxDbiP26339.
PRIDEiP26339.

PTM databases

PhosphoSiteiP26339.

Expressioni

Gene expression databases

BgeeiP26339.
CleanExiMM_CHGA.
ExpressionAtlasiP26339. baseline and differential.
GenevestigatoriP26339.

Interactioni

Subunit structurei

Interacts with SCG3; this interaction is optimal in conditions mimicking the lumenal milieu of the trans-Golgi network, i.e. pH 5.5 and 10 mM Ca(+2).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SOD1P004415EBI-990900,EBI-990792From a different organism.

Protein-protein interaction databases

IntActiP26339. 3 interactions.
STRINGi10090.ENSMUSP00000021610.

Structurei

3D structure databases

ProteinModelPortaliP26339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi103 – 11614Poly-GlnAdd
BLAST
Compositional biasi236 – 24510Poly-Glu
Compositional biasi340 – 3478Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG41926.
GeneTreeiENSGT00730000111266.
HOGENOMiHOG000111808.
HOVERGENiHBG001272.
InParanoidiP26339.
OMAiVNSPMNK.
OrthoDBiEOG7V766Z.
PhylomeDBiP26339.
TreeFamiTF336596.

Family and domain databases

InterProiIPR001819. Chromogranin_AB.
IPR018054. Chromogranin_CS.
IPR001990. Granin.
[Graphical view]
PANTHERiPTHR10583. PTHR10583. 1 hit.
PfamiPF01271. Granin. 2 hits.
[Graphical view]
PRINTSiPR00659. CHROMOGRANIN.
PROSITEiPS00422. GRANINS_1. 1 hit.
PS00423. GRANINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26339-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSTAVLALL LCAGQVFALP VNSPMTKGDT KVMKCVLEVI SDSLSKPSPM
60 70 80 90 100
PVSPECLETL QGDERILSIL RHQNLLKELQ DLALQGAKER AQQPLKQQQP
110 120 130 140 150
PKQQQQQQQQ QQQEQQHSSF EDELSEVFEN QSPDAKHRDA AAEVPSRDTM
160 170 180 190 200
EKRKDSDKGQ QDGFEATTEG PRPQAFPEPN QESPMMGDSE SPGEDTATNT
210 220 230 240 250
QSPTSLPSQE HVDPQATGDS ERGLSAQQQA RKAKQEEKEE EEEEEAVARE
260 270 280 290 300
KAGPEEVPTA ASSSHFHAGY KAIQKDDGQS DSQAVDGDGK TEASEALPSE
310 320 330 340 350
GKGELEHSQQ EEDGEEAMVG TPQGLFPQGG KGRELEHKQE EEEEEEERLS
360 370 380 390 400
REWEDKRWSR MDQLAKELTA EKRLEGEDDP DRSMKLSFRT RAYGFRDPGP
410 420 430 440 450
QLRRGWRPSS REDSVEARSD FEEKKEEEGS ANRRAEDQEL ESLSAIEAEL
460
EKVAHQLQAL RRG
Length:463
Mass (Da):51,789
Last modified:May 1, 1992 - v1
Checksum:i1AB3C5FF433C39E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64278 mRNA. Translation: AAA37457.1.
BC026554 mRNA. Translation: AAH26554.1.
CCDSiCCDS26120.1.
PIRiA39868.
RefSeqiNP_031719.1. NM_007693.1.
UniGeneiMm.4137.

Genome annotation databases

EnsembliENSMUST00000021610; ENSMUSP00000021610; ENSMUSG00000021194.
GeneIDi12652.
KEGGimmu:12652.
UCSCiuc007oui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64278 mRNA. Translation: AAA37457.1.
BC026554 mRNA. Translation: AAH26554.1.
CCDSiCCDS26120.1.
PIRiA39868.
RefSeqiNP_031719.1. NM_007693.1.
UniGeneiMm.4137.

3D structure databases

ProteinModelPortaliP26339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP26339. 3 interactions.
STRINGi10090.ENSMUSP00000021610.

PTM databases

PhosphoSiteiP26339.

Proteomic databases

MaxQBiP26339.
PaxDbiP26339.
PRIDEiP26339.

Protocols and materials databases

DNASUi12652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021610; ENSMUSP00000021610; ENSMUSG00000021194.
GeneIDi12652.
KEGGimmu:12652.
UCSCiuc007oui.1. mouse.

Organism-specific databases

CTDi1113.
MGIiMGI:88394. Chga.

Phylogenomic databases

eggNOGiNOG41926.
GeneTreeiENSGT00730000111266.
HOGENOMiHOG000111808.
HOVERGENiHBG001272.
InParanoidiP26339.
OMAiVNSPMNK.
OrthoDBiEOG7V766Z.
PhylomeDBiP26339.
TreeFamiTF336596.

Miscellaneous databases

NextBioi281864.
PROiP26339.
SOURCEiSearch...

Gene expression databases

BgeeiP26339.
CleanExiMM_CHGA.
ExpressionAtlasiP26339. baseline and differential.
GenevestigatoriP26339.

Family and domain databases

InterProiIPR001819. Chromogranin_AB.
IPR018054. Chromogranin_CS.
IPR001990. Granin.
[Graphical view]
PANTHERiPTHR10583. PTHR10583. 1 hit.
PfamiPF01271. Granin. 2 hits.
[Graphical view]
PRINTSiPR00659. CHROMOGRANIN.
PROSITEiPS00422. GRANINS_1. 1 hit.
PS00423. GRANINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of the chromogranin A gene. Clues to evolution and tissue-specific expression."
    Wu H.J., Rozansky D.J., Parmer R.J., Gill B.M., O'Connor D.T.
    J. Biol. Chem. 266:13130-13134(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  3. "Identification of a chromogranin A domain that mediates binding to secretogranin III and targeting to secretory granules in pituitary cells and pancreatic beta-cells."
    Hosaka M., Watanabe T., Sakai Y., Uchiyama Y., Takeuchi T.
    Mol. Biol. Cell 13:3388-3399(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCG3, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCMGA_MOUSE
AccessioniPrimary (citable) accession number: P26339
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds calcium with a low-affinity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.