ID   FLI1_MOUSE              Reviewed;         452 AA.
AC   P26323;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   27-MAR-2024, entry version 190.
DE   RecName: Full=Friend leukemia integration 1 transcription factor;
DE   AltName: Full=Retroviral integration site protein Fli-1;
GN   Name=Fli1; Synonyms=Fli-1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INVOLVEMENT IN ERYTHROLEUKEMIA.
RC   STRAIN=BALB/cJ; TISSUE=Spleen;
RX   PubMed=2044959; DOI=10.1101/gad.5.6.908;
RA   Ben-David Y., Giddens E.B., Letwin K., Bernstein A.;
RT   "Erythroleukemia induction by Friend murine leukemia virus: insertional
RT   activation of a new member of the ets gene family, Fli-1, closely linked to
RT   c-ets-1.";
RL   Genes Dev. 5:908-918(1991).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8502483;
RA   Zhang L., Lemarchandel V., Romeo P.-H., Ben-David Y., Greer P.,
RA   Bernstein A.;
RT   "The Fli-1 proto-oncogene, involved in erythroleukemia and Ewing's sarcoma,
RT   encodes a transcriptional activator with DNA-binding specificities distinct
RT   from other Ets family members.";
RL   Oncogene 8:1621-1630(1993).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Lung, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Sequence-specific transcriptional activator. Recognizes the
CC       DNA sequence 5'-C[CA]GGAAGT-3'. {ECO:0000250|UniProtKB:Q01543}.
CC   -!- SUBUNIT: Can form homodimers or heterodimers with ETV6/TEL1.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01543}.
CC   -!- TISSUE SPECIFICITY: Expressed in both hematopoietic and
CC       nonhematopoietic tissues.
CC   -!- INDUCTION: Specifically up-regulated in leukemic clones with F-MuLV
CC       insertions up-stream of the FLI-1 locus.
CC   -!- DISEASE: Note=Involved in erythroleukemia induction by Friend murine
CC       leukemia virus (F-MULV). {ECO:0000269|PubMed:2044959}.
CC   -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR   EMBL; X59421; CAA42055.1; -; mRNA.
DR   CCDS; CCDS40571.1; -.
DR   PIR; S17403; S17403.
DR   RefSeq; NP_032052.1; NM_008026.5.
DR   AlphaFoldDB; P26323; -.
DR   BMRB; P26323; -.
DR   SMR; P26323; -.
DR   BioGRID; 199702; 2.
DR   IntAct; P26323; 2.
DR   STRING; 10090.ENSMUSP00000016231; -.
DR   iPTMnet; P26323; -.
DR   PhosphoSitePlus; P26323; -.
DR   EPD; P26323; -.
DR   jPOST; P26323; -.
DR   PaxDb; 10090-ENSMUSP00000016231; -.
DR   PeptideAtlas; P26323; -.
DR   ProteomicsDB; 267594; -.
DR   ABCD; P26323; 83 sequenced antibodies.
DR   Antibodypedia; 9350; 780 antibodies from 43 providers.
DR   DNASU; 14247; -.
DR   Ensembl; ENSMUST00000016231.14; ENSMUSP00000016231.7; ENSMUSG00000016087.14.
DR   GeneID; 14247; -.
DR   KEGG; mmu:14247; -.
DR   UCSC; uc009orz.2; mouse.
DR   AGR; MGI:95554; -.
DR   CTD; 2313; -.
DR   MGI; MGI:95554; Fli1.
DR   VEuPathDB; HostDB:ENSMUSG00000016087; -.
DR   eggNOG; KOG3806; Eukaryota.
DR   GeneTree; ENSGT00940000158261; -.
DR   HOGENOM; CLU_045216_0_1_1; -.
DR   InParanoid; P26323; -.
DR   OMA; AXSLLAY; -.
DR   OrthoDB; 2882052at2759; -.
DR   PhylomeDB; P26323; -.
DR   TreeFam; TF350537; -.
DR   BioGRID-ORCS; 14247; 6 hits in 82 CRISPR screens.
DR   ChiTaRS; Fli1; mouse.
DR   PRO; PR:P26323; -.
DR   Proteomes; UP000000589; Chromosome 9.
DR   RNAct; P26323; Protein.
DR   Bgee; ENSMUSG00000016087; Expressed in peripheral lymph node and 230 other cell types or tissues.
DR   ExpressionAtlas; P26323; baseline and differential.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0016604; C:nuclear body; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:MGI.
DR   GO; GO:0009887; P:animal organ morphogenesis; IMP:MGI.
DR   GO; GO:0008015; P:blood circulation; IMP:MGI.
DR   GO; GO:0035855; P:megakaryocyte development; IMP:MGI.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; ISO:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IMP:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd08541; SAM_PNT-FLI-1; 1.
DR   Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000418; Ets_dom.
DR   InterPro; IPR046328; ETS_fam.
DR   InterPro; IPR003118; Pointed_dom.
DR   InterPro; IPR013761; SAM/pointed_sf.
DR   InterPro; IPR035573; SAM_PNT-FLI-1.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR11849; ETS; 1.
DR   PANTHER; PTHR11849:SF161; FRIEND LEUKEMIA INTEGRATION 1 TRANSCRIPTION FACTOR; 1.
DR   Pfam; PF00178; Ets; 1.
DR   Pfam; PF02198; SAM_PNT; 1.
DR   PRINTS; PR00454; ETSDOMAIN.
DR   SMART; SM00413; ETS; 1.
DR   SMART; SM00251; SAM_PNT; 1.
DR   SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR   PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR   PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR   PROSITE; PS51433; PNT; 1.
DR   Genevisible; P26323; MM.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Nucleus; Phosphoprotein; Proto-oncogene;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..452
FT                   /note="Friend leukemia integration 1 transcription factor"
FT                   /id="PRO_0000204125"
FT   DOMAIN          112..198
FT                   /note="PNT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00762"
FT   DNA_BIND        281..361
FT                   /note="ETS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT   REGION          236..260
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         39
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
SQ   SEQUENCE   452 AA;  51002 MW;  1F9A06C6893FE2AB CRC64;
     MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ
     PVRVNVKREY DHMNGSRESP VDCSVSKCNK LVGGGEANPM NYNSYMDEKN GPPPPNMTTN
     ERRVIVPADP TLWTQEHVRQ WLEWAIKEYG LMEIDTSFFQ NMDGKELCKM NKEDFLRATS
     AYNTEVLLSH LSYLRESSLL AYNTTSHTDQ SSRLNVKEDP SYDSVRRGAW NNNMNSGLNK
     SPLLGGSQTM GKNTEQRPQP DPYQILGPTS SRLANPGSGQ IQLWQFLLEL LSDSANASCI
     TWEGTNGEFK MTDPDEVARR WGERKSKPNM NYDKLSRALR YYYDKNIMTK VHGKRYAYKF
     DFHGIAQALQ PHPTETSMYK YPSDISYMPS YHAHQQKVNF VPSHPSSMPV TSSSFFGAAS
     QYWTSPTAGI YPNPSVPRHP NTHVPSHLGS YY
//