ID CDC20_YEAST Reviewed; 610 AA. AC P26309; D6VU31; DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1994, sequence version 2. DT 27-MAR-2024, entry version 213. DE RecName: Full=APC/C activator protein CDC20; DE AltName: Full=Cell division control protein 20; GN Name=CDC20; OrderedLocusNames=YGL116W; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204626 / S288c / A364A; RX PubMed=1922065; DOI=10.1128/mcb.11.11.5592-5602.1991; RA Sethi N., Monteagudo M.C., Koshland D., Hogan E., Burke D.J.; RT "The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin RT homolog, is required for a subset of microtubule-dependent cellular RT processes."; RL Mol. Cell. Biol. 11:5592-5602(1991). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-610. RA Doi A., Doi K.; RT "Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae."; RL Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases. RN [5] RP ASSOCIATION WITH THE APC/C COMPLEX. RX PubMed=9501986; DOI=10.1016/s0960-9822(98)70088-0; RA Lim H.H., Goh P.-Y., Surana U.; RT "Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and RT Clb2 during M phase in budding yeast."; RL Curr. Biol. 8:231-234(1998). RN [6] RP DEVELOPMENTAL STAGE, AND DOMAIN D-BOX MOTIF. RX PubMed=9651679; DOI=10.1016/s0960-9822(98)70298-2; RA Prinz S., Hwang E.S., Visintin R., Amon A.; RT "The regulation of Cdc20 proteolysis reveals a role for APC components RT Cdc23 and Cdc27 during S phase and early mitosis."; RL Curr. Biol. 8:750-760(1998). RN [7] RP INTERACTION WITH MAD2 AND MAD3, AND MUTAGENESIS OF TYR-205; PRO-209 AND RP PRO-210. RX PubMed=9461437; DOI=10.1126/science.279.5353.1041; RA Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S., RA Amon A., Murray A.W.; RT "Budding yeast Cdc20: a target of the spindle checkpoint."; RL Science 279:1041-1044(1998). RN [8] RP INTERACTION WITH MAD3. RX PubMed=10704439; DOI=10.1083/jcb.148.5.871; RA Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W.; RT "MAD3 encodes a novel component of the spindle checkpoint which interacts RT with Bub3p, Cdc20p, and Mad2p."; RL J. Cell Biol. 148:871-882(2000). RN [9] RP INTERACTION WITH PDS1. RX PubMed=11553328; DOI=10.1016/s0960-9822(01)00399-2; RA Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.; RT "The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in RT a destruction box-dependent manner."; RL Curr. Biol. 11:1347-1352(2001). RN [10] RP INTERACTION WITH PDS1, AND DOMAIN C-BOX MOTIF. RX PubMed=11566880; DOI=10.1093/emboj/20.18.5165; RA Schwab M., Neutzner M., Moecker D., Seufert W.; RT "Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the RT ubiquitin ligase APC."; RL EMBO J. 20:5165-5175(2001). RN [11] RP INTERACTION WITH HSL1. RX PubMed=11562348; DOI=10.1101/gad.917901; RA Burton J.L., Solomon M.J.; RT "D box and KEN box motifs in budding yeast Hsl1p are required for APC- RT mediated degradation and direct binding to Cdc20p and Cdh1p."; RL Genes Dev. 15:2381-2395(2001). RN [12] RP SUBCELLULAR LOCATION. RX PubMed=12456658; DOI=10.1093/emboj/cdf634; RA Jaquenoud M., van Drogen F., Peter M.; RT "Cell cycle-dependent nuclear export of Cdh1p may contribute to the RT inactivation of APC/C(Cdh1)."; RL EMBO J. 21:6515-6526(2002). RN [13] RP FUNCTION IN CLB2 AND CLB5 DEGRADATION. RX PubMed=12152084; DOI=10.1038/nature00856; RA Waesch R., Cross F.R.; RT "APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for RT mitotic exit."; RL Nature 418:556-562(2002). RN [14] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [15] RP PHOSPHORYLATION BY CDC28. RX PubMed=14574415; DOI=10.1038/nature02062; RA Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., RA Shokat K.M., Morgan D.O.; RT "Targets of the cyclin-dependent kinase Cdk1."; RL Nature 425:859-864(2003). RN [16] RP FUNCTION AS SPINDLE CHECKPOINT TARGET. RX PubMed=15198982; DOI=10.1101/gad.1184204; RA Pan J., Chen R.-H.; RT "Spindle checkpoint regulates Cdc20p stability in Saccharomyces RT cerevisiae."; RL Genes Dev. 18:1439-1451(2004). RN [17] RP IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 RP COMPLEX, AND FUNCTION OF THE MCC COMPLEX. RX PubMed=15879521; DOI=10.1128/ec.4.5.867-878.2005; RA Poddar A., Stukenberg P.T., Burke D.J.; RT "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 RT assemble during mitosis independently of the kinetochore in Saccharomyces RT cerevisiae."; RL Eukaryot. Cell 4:867-878(2005). CC -!- FUNCTION: Activator protein that regulates the ubiquitin ligase CC activity and substrate specificity of the anaphase promoting CC complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, CC recognizes and binds proteins containing a D-box including the B-type CC cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C- CC box-dependent manner to the APC/C for ubiquitination and subsequent CC proteolysis. Required for sister chromatid separation and disassembly CC of the mitotic spindle. Target of the spindle checkpoint pathway CC through participation in the mitotic checkpoint complex (MCC) and the CC MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex CC inhibit the ubiquitin ligase activity of the anaphase promoting CC complex/cyclosome (APC/C) by preventing its activation by CDC20 until CC proper attachment of all chromosomes to the spindle. CC {ECO:0000269|PubMed:12152084, ECO:0000269|PubMed:15198982, CC ECO:0000269|PubMed:15879521}. CC -!- SUBUNIT: Component of the mitotic checkpoint complex (MCC) which CC consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 CC subcomplex, both of which appear to be assembled during mitoisis CC independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 CC occurs in a larger amount than MCC. MCC associates with the with the CC APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1. CC {ECO:0000269|PubMed:10704439, ECO:0000269|PubMed:11553328, CC ECO:0000269|PubMed:11562348, ECO:0000269|PubMed:11566880, CC ECO:0000269|PubMed:15879521, ECO:0000269|PubMed:9461437}. CC -!- INTERACTION: CC P26309; P41695: BUB1; NbExp=2; IntAct=EBI-4212, EBI-3816; CC P26309; P26449: BUB3; NbExp=8; IntAct=EBI-4212, EBI-3830; CC P26309; P40958: MAD2; NbExp=6; IntAct=EBI-4212, EBI-10362; CC P26309; P47074: MAD3; NbExp=9; IntAct=EBI-4212, EBI-10369; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12456658, CC ECO:0000269|PubMed:14562095}. CC -!- DEVELOPMENTAL STAGE: Expressed in late S and M phase of the cell cycle. CC Degraded in G1 by the APC/C in its CDH1-bound form. CC {ECO:0000269|PubMed:9651679}. CC -!- DOMAIN: The KEN box is required for the association with the APC/C CC complex. CC -!- DOMAIN: The C-box is required for the association with the APC/C CC complex. CC -!- DOMAIN: The N-terminal destruction box (D-box) acts as a recognition CC signal for degradation via the ubiquitin-proteasome pathway. CC -!- PTM: Ubiquitinated by the APC/C complex. CC -!- PTM: Phosphorylated by CDC28. {ECO:0000269|PubMed:14574415}. CC -!- SIMILARITY: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CAA42058.1; Type=Frameshift; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X59428; CAA42058.1; ALT_FRAME; Genomic_DNA. DR EMBL; Z72638; CAA96824.1; -; Genomic_DNA. DR EMBL; D16506; BAA03957.1; -; Genomic_DNA. DR EMBL; BK006941; DAA07992.1; -; Genomic_DNA. DR PIR; S64126; S64126. DR RefSeq; NP_011399.1; NM_001180981.1. DR AlphaFoldDB; P26309; -. DR SMR; P26309; -. DR BioGRID; 33135; 1010. DR ComplexPortal; CPX-760; Anaphase-Promoting Complex, CDC20 variant. DR ComplexPortal; CPX-962; Mitotic checkpoint complex, CDC20-MAD2 subcomplex. DR ComplexPortal; CPX-963; Mitotic checkpoint complex, MAD2-MAD3-BUB3-CDC20. DR DIP; DIP-982N; -. DR ELM; P26309; -. DR IntAct; P26309; 23. DR MINT; P26309; -. DR STRING; 4932.YGL116W; -. DR iPTMnet; P26309; -. DR PaxDb; 4932-YGL116W; -. DR PeptideAtlas; P26309; -. DR EnsemblFungi; YGL116W_mRNA; YGL116W; YGL116W. DR GeneID; 852762; -. DR KEGG; sce:YGL116W; -. DR AGR; SGD:S000003084; -. DR SGD; S000003084; CDC20. DR VEuPathDB; FungiDB:YGL116W; -. DR eggNOG; KOG0305; Eukaryota. DR GeneTree; ENSGT00950000183104; -. DR HOGENOM; CLU_014831_6_0_1; -. DR InParanoid; P26309; -. DR OMA; TWSDDDC; -. DR OrthoDB; 20041at2759; -. DR BioCyc; YEAST:G3O-30614-MONOMER; -. DR Reactome; R-SCE-141405; Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components. DR Reactome; R-SCE-141430; Inactivation of APC/C via direct inhibition of the APC/C complex. DR Reactome; R-SCE-983168; Antigen processing: Ubiquitination & Proteasome degradation. DR BioGRID-ORCS; 852762; 1 hit in 10 CRISPR screens. DR PRO; PR:P26309; -. DR Proteomes; UP000002311; Chromosome VII. DR RNAct; P26309; Protein. DR GO; GO:0005680; C:anaphase-promoting complex; IPI:SGD. DR GO; GO:0005737; C:cytoplasm; HDA:SGD. DR GO; GO:0033597; C:mitotic checkpoint complex; IDA:SGD. DR GO; GO:1990333; C:mitotic checkpoint complex, CDC20-MAD2 subcomplex; IPI:ComplexPortal. DR GO; GO:0034399; C:nuclear periphery; IDA:SGD. DR GO; GO:0005634; C:nucleus; HDA:SGD. DR GO; GO:0010997; F:anaphase-promoting complex binding; IDA:SGD. DR GO; GO:1990757; F:ubiquitin ligase activator activity; IDA:SGD. DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IMP:SGD. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IPI:SGD. DR GO; GO:1905786; P:positive regulation of anaphase-promoting complex-dependent catabolic process; IMP:SGD. DR GO; GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; IMP:SGD. DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IDA:SGD. DR GO; GO:0016567; P:protein ubiquitination; NAS:ComplexPortal. DR GO; GO:0040020; P:regulation of meiotic nuclear division; IMP:SGD. DR GO; GO:0007346; P:regulation of mitotic cell cycle; NAS:ComplexPortal. DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1. DR InterPro; IPR033010; Cdc20/Fizzy. DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam. DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf. DR InterPro; IPR019775; WD40_repeat_CS. DR InterPro; IPR001680; WD40_rpt. DR PANTHER; PTHR19918; CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED; 1. DR PANTHER; PTHR19918:SF68; FI02843P; 1. DR Pfam; PF00400; WD40; 3. DR SMART; SM00320; WD40; 6. DR SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1. DR PROSITE; PS00678; WD_REPEATS_1; 4. DR PROSITE; PS50082; WD_REPEATS_2; 2. DR PROSITE; PS50294; WD_REPEATS_REGION; 1. PE 1: Evidence at protein level; KW Cell cycle; Cell division; Mitosis; Nucleus; Phosphoprotein; KW Reference proteome; Repeat; Ubl conjugation; WD repeat. FT CHAIN 1..610 FT /note="APC/C activator protein CDC20" FT /id="PRO_0000050903" FT REPEAT 257..296 FT /note="WD 1" FT REPEAT 299..338 FT /note="WD 2" FT REPEAT 342..379 FT /note="WD 3" FT REPEAT 383..422 FT /note="WD 4" FT REPEAT 425..467 FT /note="WD 5" FT REPEAT 469..519 FT /note="WD 6" FT REPEAT 523..562 FT /note="WD 7" FT REGION 38..59 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 579..610 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 17..25 FT /note="D-box" FT MOTIF 85..101 FT /note="Bipartite nuclear localization signal" FT MOTIF 144..150 FT /note="C-box" FT MOTIF 586..592 FT /note="KEN box" FT /evidence="ECO:0000255" FT COMPBIAS 580..596 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MUTAGEN 205 FT /note="Y->N: Confers spindle checkpoint resistance and FT diminishes binding to MAD2 and MAD3." FT /evidence="ECO:0000269|PubMed:9461437" FT MUTAGEN 209 FT /note="P->Q: Confers spindle checkpoint resistance and FT diminishes binding to MAD2 and MAD3." FT /evidence="ECO:0000269|PubMed:9461437" FT MUTAGEN 210 FT /note="P->L,S: Confers spindle checkpoint resistance and FT diminishes binding to MAD2 and MAD3." FT /evidence="ECO:0000269|PubMed:9461437" FT CONFLICT 318..319 FT /note="IG -> MA (in Ref. 1; CAA42058)" FT /evidence="ECO:0000305" SQ SEQUENCE 610 AA; 67360 MW; CCE7CD149C1F5ACF CRC64; MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS SSLNIRNSKR PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD KATFSAYSSR SYPTIGSESV VSQTSLSQPT TSREVDEQFT VAADRYIPIL QGASQNKVDP ETLHEALPPP NASPISHLRA QTKIVFKQNV AEACGLDMNK RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI KLRKINTNPE RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL SWLDTLIATG SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL ASGGNDNTVM IWDTRTSLPQ FSKKTHTAAV KALSWCPYSP NILASGGGQT DKHIHFWNSI TGARVGSINT GSQVSSLHWG QSHTSTNGGM MNKEIVATGG NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL ATVGGDENLK FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR PSSTSQYLIR //