APC/C activator protein CDC20 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P26309 (CDC20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
APC/C activator protein CDC20

Alternative name(s):
Cell division control protein 20

Gene names

Name:	CDC20
Ordered Locus Names:	YGL116W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle. Ref.13 Ref.16 Ref.17
Subunit structure	Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.17
Subcellular location	Nucleus Ref.12 Ref.14.
Developmental stage	Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form. Ref.6
Domain	The KEN box is required for the association with the APC/C complex. Ref.6 Ref.10 The C-box is required for the association with the APC/C complex. Ref.6 Ref.10 The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway. Ref.6 Ref.10
Post-translational modification	Ubiquitinated by the APC/C complex. Phosphorylated by CDC28. Ref.15
Sequence similarities	Belongs to the WD repeat CDC20/Fizzy family. Contains 7 WD repeats.
Sequence caution	The sequence CAA42058.1 differs from that shown. Reason: Frameshift at position 502.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity involved in meiotic cell cycle Inferred from mutant phenotype PubMed 17889668. Source: SGD activation of mitotic anaphase-promoting complex activity Inferred from mutant phenotype PubMed 9334304 PubMed 9504909. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW cyclin catabolic process Inferred from mutant phenotype Ref.13. Source: SGD meiotic anaphase I Inferred from mutant phenotype PubMed 10855492. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW spindle checkpoint Inferred from physical interaction Ref.7. Source: SGD
Cellular_component	anaphase-promoting complex Inferred from physical interaction Ref.5. Source: SGD cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD mitotic checkpoint complex Inferred from direct assay PubMed 11726501. Source: SGD
Molecular_function	anaphase-promoting complex binding Inferred from direct assay PubMed 15916961. Source: SGD ubiquitin-protein ligase activator activity Inferred from direct assay PubMed 19362536. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
BUB1	P41695	2	EBI-4212,EBI-3816
BUB3	P26449	8	EBI-4212,EBI-3830
MAD2	P40958	6	EBI-4212,EBI-10362
MAD3	P47074	9	EBI-4212,EBI-10369

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 610

610

APC/C activator protein CDC20

PRO_0000050903

Regions

Repeat

257 – 296

WD 1

Repeat

299 – 338

WD 2

Repeat

342 – 379

WD 3

Repeat

383 – 422

WD 4

Repeat

425 – 467

WD 5

Repeat

469 – 519

WD 6

Repeat

523 – 562

WD 7

Motif

17 – 25

D-box

Motif

85 – 101

Bipartite nuclear localization signal

Motif

144 – 150

C-box

Motif

586 – 592

KEN box Potential

Amino acid modifications

Modified residue

146

Phosphotyrosine Ref.18

Experimental info

Mutagenesis

205

Y → N: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7

Mutagenesis

209

P → Q: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7

Mutagenesis

210

P → L or S: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7

Sequence conflict

318 – 319

IG → MA in CAA42058. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P26309 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: CCE7CD149C1F5ACF
Show »

FASTA

610

67,360

        10         20         30         40         50         60 
MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS SSLNIRNSKR 

        70         80         90        100        110        120 
PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD KATFSAYSSR SYPTIGSESV 

       130        140        150        160        170        180 
VSQTSLSQPT TSREVDEQFT VAADRYIPIL QGASQNKVDP ETLHEALPPP NASPISHLRA 

       190        200        210        220        230        240 
QTKIVFKQNV AEACGLDMNK RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI 

       250        260        270        280        290        300 
KLRKINTNPE RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE 

       310        320        330        340        350        360 
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL SWLDTLIATG 

       370        380        390        400        410        420 
SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL ASGGNDNTVM IWDTRTSLPQ 

       430        440        450        460        470        480 
FSKKTHTAAV KALSWCPYSP NILASGGGQT DKHIHFWNSI TGARVGSINT GSQVSSLHWG 

       490        500        510        520        530        540 
QSHTSTNGGM MNKEIVATGG NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL 

       550        560        570        580        590        600 
ATVGGDENLK FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR 

       610 
PSSTSQYLIR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes." Sethi N., Monteagudo M.C., Koshland D., Hogan E., Burke D.J. Mol. Cell. Biol. 11:5592-5602(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae." Doi A., Doi K. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-610.
[5]	"Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast." Lim H.H., Goh P.-Y., Surana U. Curr. Biol. 8:231-234(1998) [PubMed] [Europe PMC] [Abstract] Cited for: ASSOCIATION WITH THE APC/C COMPLEX.
[6]	"The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis." Prinz S., Hwang E.S., Visintin R., Amon A. Curr. Biol. 8:750-760(1998) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE, DOMAIN D-BOX MOTIF.
[7]	"Budding yeast Cdc20: a target of the spindle checkpoint." Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S., Amon A., Murray A.W. Science 279:1041-1044(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAD2 AND MAD3, MUTAGENESIS OF TYR-205; PRO-209 AND PRO-210.
[8]	"MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p." Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W. J. Cell Biol. 148:871-882(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAD3.
[9]	"The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner." Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O. Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PDS1.
[10]	"Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC." Schwab M., Neutzner M., Moecker D., Seufert W. EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PDS1, DOMAIN C-BOX MOTIF.
[11]	"D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p." Burton J.L., Solomon M.J. Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HSL1.
[12]	"Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)." Jaquenoud M., van Drogen F., Peter M. EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[13]	"APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit." Waesch R., Cross F.R. Nature 418:556-562(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CLB2 AND CLB5 DEGRADATION.
[14]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[15]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[16]	"Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae." Pan J., Chen R.-H. Genes Dev. 18:1439-1451(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
[17]	"Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae." Poddar A., Stukenberg P.T., Burke D.J. Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.
[18]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-146, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59428 Genomic DNA. Translation: CAA42058.1. Frameshift. Z72638 Genomic DNA. Translation: CAA96824.1. D16506 Genomic DNA. Translation: BAA03957.1. BK006941 Genomic DNA. Translation: DAA07992.1.
PIR	S64126.
RefSeq	NP_011399.1. NM_001180981.1.
3D structure databases
ProteinModelPortal	P26309.
SMR	P26309. Positions 243-556.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-982N.
IntAct	P26309. 18 interactions.
MINT	MINT-401776.
STRING	4932.YGL116W.
Proteomic databases
PaxDb	P26309.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL116W; YGL116W; YGL116W.
GeneID	852762.
KEGG	sce:YGL116W.
Organism-specific databases
CYGD	YGL116w.
SGD	S000003084. CDC20.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00580000081599.
HOGENOM	HOG000195514.
KO	K03363.
OMA	QVSSLHW.
OrthoDB	EOG4KD9V8.
Gene expression databases
Genevestigator	P26309.
GermOnline	YGL116W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 4 hits. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972212.

Entry information

Entry name

CDC20_YEAST

Accession

Primary (citable) accession number: P26309
Secondary accession number(s): D6VU31

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents