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P26309

- CDC20_YEAST

UniProt

P26309 - CDC20_YEAST

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Protein

APC/C activator protein CDC20

Gene

CDC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.3 Publications

GO - Molecular functioni

  1. anaphase-promoting complex binding Source: SGD
  2. ubiquitin-protein transferase activator activity Source: SGD

GO - Biological processi

  1. activation of anaphase-promoting complex activity involved in meiotic cell cycle Source: SGD
  2. activation of mitotic anaphase-promoting complex activity Source: SGD
  3. cyclin catabolic process Source: SGD
  4. mitotic nuclear division Source: UniProtKB-KW
  5. mitotic spindle assembly checkpoint Source: SGD
  6. positive regulation of mitotic metaphase/anaphase transition Source: SGD
  7. positive regulation of protein catabolic process Source: SGD
  8. regulation of meiosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30614-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
APC/C activator protein CDC20
Alternative name(s):
Cell division control protein 20
Gene namesi
Name:CDC20
Ordered Locus Names:YGL116W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL116w.
SGDiS000003084. CDC20.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. anaphase-promoting complex Source: SGD
  2. mitotic checkpoint complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi205 – 2051Y → N: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication
Mutagenesisi209 – 2091P → Q: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication
Mutagenesisi210 – 2101P → L or S: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610APC/C activator protein CDC20PRO_0000050903Add
BLAST

Post-translational modificationi

Ubiquitinated by the APC/C complex.
Phosphorylated by CDC28.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP26309.

Expressioni

Developmental stagei

Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form.1 Publication

Gene expression databases

GenevestigatoriP26309.

Interactioni

Subunit structurei

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB1P416952EBI-4212,EBI-3816
BUB3P264498EBI-4212,EBI-3830
MAD2P409586EBI-4212,EBI-10362
MAD3P470748EBI-4212,EBI-10369

Protein-protein interaction databases

BioGridi33135. 251 interactions.
DIPiDIP-982N.
IntActiP26309. 12 interactions.
MINTiMINT-401776.
STRINGi4932.YGL116W.

Structurei

3D structure databases

ProteinModelPortaliP26309.
SMRiP26309. Positions 243-556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati257 – 29640WD 1Add
BLAST
Repeati299 – 33840WD 2Add
BLAST
Repeati342 – 37938WD 3Add
BLAST
Repeati383 – 42240WD 4Add
BLAST
Repeati425 – 46743WD 5Add
BLAST
Repeati469 – 51951WD 6Add
BLAST
Repeati523 – 56240WD 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi17 – 259D-box
Motifi85 – 10117Bipartite nuclear localization signalAdd
BLAST
Motifi144 – 1507C-box
Motifi586 – 5927KEN boxSequence Analysis

Domaini

The KEN box is required for the association with the APC/C complex.
The C-box is required for the association with the APC/C complex.
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000119352.
HOGENOMiHOG000195514.
InParanoidiP26309.
KOiK03363.
OMAiSSLHWGQ.
OrthoDBiEOG7MSMZF.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26309-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS
60 70 80 90 100
SSLNIRNSKR PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD
110 120 130 140 150
KATFSAYSSR SYPTIGSESV VSQTSLSQPT TSREVDEQFT VAADRYIPIL
160 170 180 190 200
QGASQNKVDP ETLHEALPPP NASPISHLRA QTKIVFKQNV AEACGLDMNK
210 220 230 240 250
RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI KLRKINTNPE
260 270 280 290 300
RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE
310 320 330 340 350
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL
360 370 380 390 400
SWLDTLIATG SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL
410 420 430 440 450
ASGGNDNTVM IWDTRTSLPQ FSKKTHTAAV KALSWCPYSP NILASGGGQT
460 470 480 490 500
DKHIHFWNSI TGARVGSINT GSQVSSLHWG QSHTSTNGGM MNKEIVATGG
510 520 530 540 550
NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL ATVGGDENLK
560 570 580 590 600
FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR
610
PSSTSQYLIR
Length:610
Mass (Da):67,360
Last modified:February 1, 1994 - v2
Checksum:iCCE7CD149C1F5ACF
GO

Sequence cautioni

The sequence CAA42058.1 differs from that shown. Reason: Frameshift at position 502. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti318 – 3192IG → MA in CAA42058. (PubMed:1922065)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59428 Genomic DNA. Translation: CAA42058.1. Frameshift.
Z72638 Genomic DNA. Translation: CAA96824.1.
D16506 Genomic DNA. Translation: BAA03957.1.
BK006941 Genomic DNA. Translation: DAA07992.1.
PIRiS64126.
RefSeqiNP_011399.1. NM_001180981.1.

Genome annotation databases

EnsemblFungiiYGL116W; YGL116W; YGL116W.
GeneIDi852762.
KEGGisce:YGL116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59428 Genomic DNA. Translation: CAA42058.1 . Frameshift.
Z72638 Genomic DNA. Translation: CAA96824.1 .
D16506 Genomic DNA. Translation: BAA03957.1 .
BK006941 Genomic DNA. Translation: DAA07992.1 .
PIRi S64126.
RefSeqi NP_011399.1. NM_001180981.1.

3D structure databases

ProteinModelPortali P26309.
SMRi P26309. Positions 243-556.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33135. 251 interactions.
DIPi DIP-982N.
IntActi P26309. 12 interactions.
MINTi MINT-401776.
STRINGi 4932.YGL116W.

Proteomic databases

PaxDbi P26309.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL116W ; YGL116W ; YGL116W .
GeneIDi 852762.
KEGGi sce:YGL116W.

Organism-specific databases

CYGDi YGL116w.
SGDi S000003084. CDC20.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00760000119352.
HOGENOMi HOG000195514.
InParanoidi P26309.
KOi K03363.
OMAi SSLHWGQ.
OrthoDBi EOG7MSMZF.

Enzyme and pathway databases

BioCyci YEAST:G3O-30614-MONOMER.

Miscellaneous databases

NextBioi 972212.
PROi P26309.

Gene expression databases

Genevestigatori P26309.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 3 hits.
[Graphical view ]
SMARTi SM00320. WD40. 6 hits.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes."
    Sethi N., Monteagudo M.C., Koshland D., Hogan E., Burke D.J.
    Mol. Cell. Biol. 11:5592-5602(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae."
    Doi A., Doi K.
    Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-610.
  5. "Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast."
    Lim H.H., Goh P.-Y., Surana U.
    Curr. Biol. 8:231-234(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH THE APC/C COMPLEX.
  6. "The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis."
    Prinz S., Hwang E.S., Visintin R., Amon A.
    Curr. Biol. 8:750-760(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, DOMAIN D-BOX MOTIF.
  7. Cited for: INTERACTION WITH MAD2 AND MAD3, MUTAGENESIS OF TYR-205; PRO-209 AND PRO-210.
  8. "MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p."
    Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W.
    J. Cell Biol. 148:871-882(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAD3.
  9. "The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
    Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.
    Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDS1.
  10. "Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
    Schwab M., Neutzner M., Moecker D., Seufert W.
    EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDS1, DOMAIN C-BOX MOTIF.
  11. "D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p."
    Burton J.L., Solomon M.J.
    Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSL1.
  12. "Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)."
    Jaquenoud M., van Drogen F., Peter M.
    EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit."
    Waesch R., Cross F.R.
    Nature 418:556-562(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CLB2 AND CLB5 DEGRADATION.
  14. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  15. Cited for: PHOSPHORYLATION BY CDC28.
  16. "Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae."
    Pan J., Chen R.-H.
    Genes Dev. 18:1439-1451(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
  17. "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
    Poddar A., Stukenberg P.T., Burke D.J.
    Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.

Entry informationi

Entry nameiCDC20_YEAST
AccessioniPrimary (citable) accession number: P26309
Secondary accession number(s): D6VU31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: November 26, 2014
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3