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P26309

- CDC20_YEAST

UniProt

P26309 - CDC20_YEAST

Protein

APC/C activator protein CDC20

Gene

CDC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.3 Publications

    GO - Molecular functioni

    1. anaphase-promoting complex binding Source: SGD
    2. protein binding Source: IntAct
    3. ubiquitin-protein transferase activator activity Source: SGD

    GO - Biological processi

    1. activation of anaphase-promoting complex activity involved in meiotic cell cycle Source: SGD
    2. activation of mitotic anaphase-promoting complex activity Source: SGD
    3. cyclin catabolic process Source: SGD
    4. mitotic nuclear division Source: UniProtKB-KW
    5. mitotic spindle assembly checkpoint Source: SGD
    6. positive regulation of mitotic metaphase/anaphase transition Source: SGD
    7. positive regulation of protein catabolic process Source: SGD
    8. regulation of meiosis Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30614-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    APC/C activator protein CDC20
    Alternative name(s):
    Cell division control protein 20
    Gene namesi
    Name:CDC20
    Ordered Locus Names:YGL116W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL116w.
    SGDiS000003084. CDC20.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD
    2. mitotic checkpoint complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi205 – 2051Y → N: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication
    Mutagenesisi209 – 2091P → Q: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication
    Mutagenesisi210 – 2101P → L or S: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 610610APC/C activator protein CDC20PRO_0000050903Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by the APC/C complex.
    Phosphorylated by CDC28.1 Publication

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP26309.

    Expressioni

    Developmental stagei

    Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form.1 Publication

    Gene expression databases

    GenevestigatoriP26309.

    Interactioni

    Subunit structurei

    Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUB1P416952EBI-4212,EBI-3816
    BUB3P264498EBI-4212,EBI-3830
    MAD2P409586EBI-4212,EBI-10362
    MAD3P470748EBI-4212,EBI-10369

    Protein-protein interaction databases

    BioGridi33135. 248 interactions.
    DIPiDIP-982N.
    IntActiP26309. 12 interactions.
    MINTiMINT-401776.
    STRINGi4932.YGL116W.

    Structurei

    3D structure databases

    ProteinModelPortaliP26309.
    SMRiP26309. Positions 243-556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati257 – 29640WD 1Add
    BLAST
    Repeati299 – 33840WD 2Add
    BLAST
    Repeati342 – 37938WD 3Add
    BLAST
    Repeati383 – 42240WD 4Add
    BLAST
    Repeati425 – 46743WD 5Add
    BLAST
    Repeati469 – 51951WD 6Add
    BLAST
    Repeati523 – 56240WD 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi17 – 259D-box
    Motifi85 – 10117Bipartite nuclear localization signalAdd
    BLAST
    Motifi144 – 1507C-box
    Motifi586 – 5927KEN boxSequence Analysis

    Domaini

    The KEN box is required for the association with the APC/C complex.
    The C-box is required for the association with the APC/C complex.
    The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00750000118716.
    HOGENOMiHOG000195514.
    KOiK03363.
    OMAiSSLHWGQ.
    OrthoDBiEOG7MSMZF.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 3 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 6 hits.
    [Graphical view]
    SUPFAMiSSF50998. SSF50998. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26309-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS    50
    SSLNIRNSKR PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD 100
    KATFSAYSSR SYPTIGSESV VSQTSLSQPT TSREVDEQFT VAADRYIPIL 150
    QGASQNKVDP ETLHEALPPP NASPISHLRA QTKIVFKQNV AEACGLDMNK 200
    RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI KLRKINTNPE 250
    RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE 300
    NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL 350
    SWLDTLIATG SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL 400
    ASGGNDNTVM IWDTRTSLPQ FSKKTHTAAV KALSWCPYSP NILASGGGQT 450
    DKHIHFWNSI TGARVGSINT GSQVSSLHWG QSHTSTNGGM MNKEIVATGG 500
    NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL ATVGGDENLK 550
    FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR 600
    PSSTSQYLIR 610
    Length:610
    Mass (Da):67,360
    Last modified:February 1, 1994 - v2
    Checksum:iCCE7CD149C1F5ACF
    GO

    Sequence cautioni

    The sequence CAA42058.1 differs from that shown. Reason: Frameshift at position 502.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti318 – 3192IG → MA in CAA42058. (PubMed:1922065)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59428 Genomic DNA. Translation: CAA42058.1. Frameshift.
    Z72638 Genomic DNA. Translation: CAA96824.1.
    D16506 Genomic DNA. Translation: BAA03957.1.
    BK006941 Genomic DNA. Translation: DAA07992.1.
    PIRiS64126.
    RefSeqiNP_011399.1. NM_001180981.1.

    Genome annotation databases

    EnsemblFungiiYGL116W; YGL116W; YGL116W.
    GeneIDi852762.
    KEGGisce:YGL116W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59428 Genomic DNA. Translation: CAA42058.1 . Frameshift.
    Z72638 Genomic DNA. Translation: CAA96824.1 .
    D16506 Genomic DNA. Translation: BAA03957.1 .
    BK006941 Genomic DNA. Translation: DAA07992.1 .
    PIRi S64126.
    RefSeqi NP_011399.1. NM_001180981.1.

    3D structure databases

    ProteinModelPortali P26309.
    SMRi P26309. Positions 243-556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33135. 248 interactions.
    DIPi DIP-982N.
    IntActi P26309. 12 interactions.
    MINTi MINT-401776.
    STRINGi 4932.YGL116W.

    Proteomic databases

    PaxDbi P26309.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL116W ; YGL116W ; YGL116W .
    GeneIDi 852762.
    KEGGi sce:YGL116W.

    Organism-specific databases

    CYGDi YGL116w.
    SGDi S000003084. CDC20.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00750000118716.
    HOGENOMi HOG000195514.
    KOi K03363.
    OMAi SSLHWGQ.
    OrthoDBi EOG7MSMZF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30614-MONOMER.

    Miscellaneous databases

    NextBioi 972212.
    PROi P26309.

    Gene expression databases

    Genevestigatori P26309.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 3 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 6 hits.
    [Graphical view ]
    SUPFAMi SSF50998. SSF50998. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes."
      Sethi N., Monteagudo M.C., Koshland D., Hogan E., Burke D.J.
      Mol. Cell. Biol. 11:5592-5602(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204626 / S288c / A364A.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae."
      Doi A., Doi K.
      Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-610.
    5. "Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast."
      Lim H.H., Goh P.-Y., Surana U.
      Curr. Biol. 8:231-234(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE APC/C COMPLEX.
    6. "The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis."
      Prinz S., Hwang E.S., Visintin R., Amon A.
      Curr. Biol. 8:750-760(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, DOMAIN D-BOX MOTIF.
    7. Cited for: INTERACTION WITH MAD2 AND MAD3, MUTAGENESIS OF TYR-205; PRO-209 AND PRO-210.
    8. "MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p."
      Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W.
      J. Cell Biol. 148:871-882(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MAD3.
    9. "The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
      Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.
      Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDS1.
    10. "Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
      Schwab M., Neutzner M., Moecker D., Seufert W.
      EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PDS1, DOMAIN C-BOX MOTIF.
    11. "D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p."
      Burton J.L., Solomon M.J.
      Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HSL1.
    12. "Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)."
      Jaquenoud M., van Drogen F., Peter M.
      EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    13. "APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit."
      Waesch R., Cross F.R.
      Nature 418:556-562(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CLB2 AND CLB5 DEGRADATION.
    14. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    15. Cited for: PHOSPHORYLATION BY CDC28.
    16. "Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae."
      Pan J., Chen R.-H.
      Genes Dev. 18:1439-1451(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
    17. "Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
      Poddar A., Stukenberg P.T., Burke D.J.
      Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.

    Entry informationi

    Entry nameiCDC20_YEAST
    AccessioniPrimary (citable) accession number: P26309
    Secondary accession number(s): D6VU31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3