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P26309 (CDC20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
APC/C activator protein CDC20
Alternative name(s):
Cell division control protein 20
Gene names
Name:CDC20
Ordered Locus Names:YGL116W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle. Ref.13 Ref.16 Ref.17

Subunit structure

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.17

Subcellular location

Nucleus Ref.12 Ref.14.

Developmental stage

Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form. Ref.6

Domain

The KEN box is required for the association with the APC/C complex. Ref.6 Ref.10

The C-box is required for the association with the APC/C complex. Ref.6 Ref.10

The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway. Ref.6 Ref.10

Post-translational modification

Ubiquitinated by the APC/C complex.

Phosphorylated by CDC28. Ref.15

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Contains 7 WD repeats.

Sequence caution

The sequence CAA42058.1 differs from that shown. Reason: Frameshift at position 502.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610APC/C activator protein CDC20
PRO_0000050903

Regions

Repeat257 – 29640WD 1
Repeat299 – 33840WD 2
Repeat342 – 37938WD 3
Repeat383 – 42240WD 4
Repeat425 – 46743WD 5
Repeat469 – 51951WD 6
Repeat523 – 56240WD 7
Motif17 – 259D-box
Motif85 – 10117Bipartite nuclear localization signal
Motif144 – 1507C-box
Motif586 – 5927KEN box Potential

Experimental info

Mutagenesis2051Y → N: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7
Mutagenesis2091P → Q: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7
Mutagenesis2101P → L or S: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. Ref.7
Sequence conflict318 – 3192IG → MA in CAA42058. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P26309 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: CCE7CD149C1F5ACF

FASTA61067,360
        10         20         30         40         50         60 
MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS SSLNIRNSKR 

        70         80         90        100        110        120 
PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD KATFSAYSSR SYPTIGSESV 

       130        140        150        160        170        180 
VSQTSLSQPT TSREVDEQFT VAADRYIPIL QGASQNKVDP ETLHEALPPP NASPISHLRA 

       190        200        210        220        230        240 
QTKIVFKQNV AEACGLDMNK RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI 

       250        260        270        280        290        300 
KLRKINTNPE RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE 

       310        320        330        340        350        360 
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL SWLDTLIATG 

       370        380        390        400        410        420 
SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL ASGGNDNTVM IWDTRTSLPQ 

       430        440        450        460        470        480 
FSKKTHTAAV KALSWCPYSP NILASGGGQT DKHIHFWNSI TGARVGSINT GSQVSSLHWG 

       490        500        510        520        530        540 
QSHTSTNGGM MNKEIVATGG NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL 

       550        560        570        580        590        600 
ATVGGDENLK FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR 

       610 
PSSTSQYLIR 

« Hide

References

« Hide 'large scale' references
[1]"The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes."
Sethi N., Monteagudo M.C., Koshland D., Hogan E., Burke D.J.
Mol. Cell. Biol. 11:5592-5602(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204626 / S288c / A364A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae."
Doi A., Doi K.
Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-610.
[5]"Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast."
Lim H.H., Goh P.-Y., Surana U.
Curr. Biol. 8:231-234(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH THE APC/C COMPLEX.
[6]"The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis."
Prinz S., Hwang E.S., Visintin R., Amon A.
Curr. Biol. 8:750-760(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, DOMAIN D-BOX MOTIF.
[7]"Budding yeast Cdc20: a target of the spindle checkpoint."
Hwang L.H., Lau L.F., Smith D.L., Mistrot C.A., Hardwick K.G., Hwang E.S., Amon A., Murray A.W.
Science 279:1041-1044(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAD2 AND MAD3, MUTAGENESIS OF TYR-205; PRO-209 AND PRO-210.
[8]"MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p."
Hardwick K.G., Johnston R.C., Smith D.L., Murray A.W.
J. Cell Biol. 148:871-882(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAD3.
[9]"The anaphase inhibitor Pds1 binds to the APC/C-associated protein Cdc20 in a destruction box-dependent manner."
Hilioti Z., Chung Y.-S., Mochizuki Y., Hardy C.F.J., Cohen-Fix O.
Curr. Biol. 11:1347-1352(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PDS1.
[10]"Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
Schwab M., Neutzner M., Moecker D., Seufert W.
EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PDS1, DOMAIN C-BOX MOTIF.
[11]"D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p."
Burton J.L., Solomon M.J.
Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HSL1.
[12]"Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)."
Jaquenoud M., van Drogen F., Peter M.
EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit."
Waesch R., Cross F.R.
Nature 418:556-562(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN CLB2 AND CLB5 DEGRADATION.
[14]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[15]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28.
[16]"Spindle checkpoint regulates Cdc20p stability in Saccharomyces cerevisiae."
Pan J., Chen R.-H.
Genes Dev. 18:1439-1451(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
[17]"Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae."
Poddar A., Stukenberg P.T., Burke D.J.
Eukaryot. Cell 4:867-878(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE MCC COMPLEX, IDENTIFICATION IN THE MAD2-CDC20 COMPLEX, FUNCTION OF THE MCC COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59428 Genomic DNA. Translation: CAA42058.1. Frameshift.
Z72638 Genomic DNA. Translation: CAA96824.1.
D16506 Genomic DNA. Translation: BAA03957.1.
BK006941 Genomic DNA. Translation: DAA07992.1.
PIRS64126.
RefSeqNP_011399.1. NM_001180981.1.

3D structure databases

ProteinModelPortalP26309.
SMRP26309. Positions 198-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33135. 247 interactions.
DIPDIP-982N.
IntActP26309. 12 interactions.
MINTMINT-401776.
STRING4932.YGL116W.

Proteomic databases

PaxDbP26309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL116W; YGL116W; YGL116W.
GeneID852762.
KEGGsce:YGL116W.

Organism-specific databases

CYGDYGL116w.
SGDS000003084. CDC20.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00750000118716.
HOGENOMHOG000195514.
KOK03363.
OMASSLHWGQ.
OrthoDBEOG7MSMZF.

Enzyme and pathway databases

BioCycYEAST:G3O-30614-MONOMER.

Gene expression databases

GenevestigatorP26309.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 3 hits.
[Graphical view]
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50998. SSF50998. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972212.
PROP26309.

Entry information

Entry nameCDC20_YEAST
AccessionPrimary (citable) accession number: P26309
Secondary accession number(s): D6VU31
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families