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Protein

APC/C activator protein CDC20

Gene

CDC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.3 Publications

GO - Molecular functioni

  • anaphase-promoting complex binding Source: SGD
  • ubiquitin-protein transferase activator activity Source: SGD

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: SGD
  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle assembly checkpoint Source: SGD
  • positive regulation of mitotic metaphase/anaphase transition Source: SGD
  • positive regulation of protein catabolic process Source: SGD
  • positive regulation of ubiquitin protein ligase activity Source: SGD
  • positive regulation of ubiquitin-protein ligase activity involved in meiotic cell cycle Source: SGD
  • regulation of meiotic nuclear division Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30614-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
APC/C activator protein CDC20
Alternative name(s):
Cell division control protein 20
Gene namesi
Name:CDC20
Ordered Locus Names:YGL116W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL116W.
SGDiS000003084. CDC20.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: SGD
  • mitotic checkpoint complex Source: SGD
  • nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi205Y → N: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication1
Mutagenesisi209P → Q: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication1
Mutagenesisi210P → L or S: Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509031 – 610APC/C activator protein CDC20Add BLAST610

Post-translational modificationi

Ubiquitinated by the APC/C complex.
Phosphorylated by CDC28.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP26309.

PTM databases

iPTMnetiP26309.

Expressioni

Developmental stagei

Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form.1 Publication

Interactioni

Subunit structurei

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB1P416952EBI-4212,EBI-3816
BUB3P264498EBI-4212,EBI-3830
MAD2P409586EBI-4212,EBI-10362
MAD3P470748EBI-4212,EBI-10369

GO - Molecular functioni

  • anaphase-promoting complex binding Source: SGD

Protein-protein interaction databases

BioGridi33135. 255 interactors.
DIPiDIP-982N.
IntActiP26309. 12 interactors.
MINTiMINT-401776.

Structurei

3D structure databases

ProteinModelPortaliP26309.
SMRiP26309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati257 – 296WD 1Add BLAST40
Repeati299 – 338WD 2Add BLAST40
Repeati342 – 379WD 3Add BLAST38
Repeati383 – 422WD 4Add BLAST40
Repeati425 – 467WD 5Add BLAST43
Repeati469 – 519WD 6Add BLAST51
Repeati523 – 562WD 7Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi17 – 25D-box9
Motifi85 – 101Bipartite nuclear localization signalAdd BLAST17
Motifi144 – 150C-box7
Motifi586 – 592KEN boxSequence analysis7

Domaini

The KEN box is required for the association with the APC/C complex.
The C-box is required for the association with the APC/C complex.
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133814.
HOGENOMiHOG000195514.
InParanoidiP26309.
KOiK03363.
OMAiHQTRIAS.
OrthoDBiEOG092C2B79.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033010. Cdc20/Fizzy.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPESSRDKGN AAISGNRSVL SIASPTKLNI LSSDWSRNQG KVSKNSLKRS
60 70 80 90 100
SSLNIRNSKR PSLQASANSI YSRPKITIGA PPLIRRDSSF FKDEFDAKKD
110 120 130 140 150
KATFSAYSSR SYPTIGSESV VSQTSLSQPT TSREVDEQFT VAADRYIPIL
160 170 180 190 200
QGASQNKVDP ETLHEALPPP NASPISHLRA QTKIVFKQNV AEACGLDMNK
210 220 230 240 250
RILQYMPEPP KCSSLRQKSY IMKKRTHYSY QQEQKIPDLI KLRKINTNPE
260 270 280 290 300
RILDAPGFQD DFYLNLLSWS KKNVLAIALD TALYLWNATT GDVSLLTDFE
310 320 330 340 350
NTTICSVTWS DDDCHISIGK EDGNTEIWDV ETMSLIRTMR SGLGVRIGSL
360 370 380 390 400
SWLDTLIATG SRSGEIQIND VRIKQHIVST WAEHTGEVCG LSYKSDGLQL
410 420 430 440 450
ASGGNDNTVM IWDTRTSLPQ FSKKTHTAAV KALSWCPYSP NILASGGGQT
460 470 480 490 500
DKHIHFWNSI TGARVGSINT GSQVSSLHWG QSHTSTNGGM MNKEIVATGG
510 520 530 540 550
NPENAISVYN YETKFKVAEV VHAHEARICC SQLSPDGTTL ATVGGDENLK
560 570 580 590 600
FYKIFDPRCT GRSREDGLMD GMLGLIGKEG CRTNDKENRS KNSSEIHTRR
610
PSSTSQYLIR
Length:610
Mass (Da):67,360
Last modified:February 1, 1994 - v2
Checksum:iCCE7CD149C1F5ACF
GO

Sequence cautioni

The sequence CAA42058 differs from that shown. Reason: Frameshift at position 502.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti318 – 319IG → MA in CAA42058 (PubMed:1922065).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59428 Genomic DNA. Translation: CAA42058.1. Frameshift.
Z72638 Genomic DNA. Translation: CAA96824.1.
D16506 Genomic DNA. Translation: BAA03957.1.
BK006941 Genomic DNA. Translation: DAA07992.1.
PIRiS64126.
RefSeqiNP_011399.1. NM_001180981.1.

Genome annotation databases

EnsemblFungiiYGL116W; YGL116W; YGL116W.
GeneIDi852762.
KEGGisce:YGL116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59428 Genomic DNA. Translation: CAA42058.1. Frameshift.
Z72638 Genomic DNA. Translation: CAA96824.1.
D16506 Genomic DNA. Translation: BAA03957.1.
BK006941 Genomic DNA. Translation: DAA07992.1.
PIRiS64126.
RefSeqiNP_011399.1. NM_001180981.1.

3D structure databases

ProteinModelPortaliP26309.
SMRiP26309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33135. 255 interactors.
DIPiDIP-982N.
IntActiP26309. 12 interactors.
MINTiMINT-401776.

PTM databases

iPTMnetiP26309.

Proteomic databases

PRIDEiP26309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL116W; YGL116W; YGL116W.
GeneIDi852762.
KEGGisce:YGL116W.

Organism-specific databases

EuPathDBiFungiDB:YGL116W.
SGDiS000003084. CDC20.

Phylogenomic databases

GeneTreeiENSGT00860000133814.
HOGENOMiHOG000195514.
InParanoidiP26309.
KOiK03363.
OMAiHQTRIAS.
OrthoDBiEOG092C2B79.

Enzyme and pathway databases

BioCyciYEAST:G3O-30614-MONOMER.

Miscellaneous databases

PROiP26309.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR033010. Cdc20/Fizzy.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDC20_YEAST
AccessioniPrimary (citable) accession number: P26309
Secondary accession number(s): D6VU31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.