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Protein

Enolase 1

Gene

ENO1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (GAPC3), Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic (GAPC1)
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase, Pyruvate kinase (ZEAMMB73_Zm00001d001831), Pyruvate kinase, Pyruvate kinase (ZEAMMB73_Zm00001d001831), Pyruvate kinase, Pyruvate kinase, Pyruvate kinase (ZEAMMB73_Zm00001d001831), Pyruvate kinase (100191365), Pyruvate kinase (100272999), Pyruvate kinase (ZEAMMB73_Zm00001d001831), Pyruvate kinase (100193244), Pyruvate kinase (100193244), Pyruvate kinase (100272951), Pyruvate kinase (ZEAMMB73_Zm00001d023379), Pyruvate kinase (100272999), Pyruvate kinase (100272999), Pyruvate kinase (606466), Pyruvate kinase, Pyruvate kinase (606466), Pyruvate kinase (606466), Pyruvate kinase (100273990), Pyruvate kinase (606466), Pyruvate kinase (606466), Pyruvate kinase (606466), Pyruvate kinase, Pyruvate kinase (100273990), Pyruvate kinase (103644177), Pyruvate kinase (100273990), Pyruvate kinase (103644177), Pyruvate kinase (100272999), Pyruvate kinase (100272999), Pyruvate kinase (100273990), Pyruvate kinase, Pyruvate kinase (100272999), Pyruvate kinase (100273990), Pyruvate kinase (100273990), Pyruvate kinase (100272951), Pyruvate kinase (ZEAMMB73_Zm00001d026619), Pyruvate kinase, Pyruvate kinase (ZEAMMB73_Zm00001d040446), Pyruvate kinase (100191614), Pyruvate kinase (100191614), Pyruvate kinase (ZEAMMB73_Zm00001d001831), Pyruvate kinase (606466), Pyruvate kinase (606466), Pyruvate kinase (606466), Pyruvate kinase, Pyruvate kinase (100193244), Pyruvate kinase (ZEAMMB73_Zm00001d023379), Pyruvate kinase (100191614), Pyruvate kinase (ZEAMMB73_Zm00001d026619), Pyruvate kinase (100191365), Pyruvate kinase (ZEAMMB73_Zm00001d026619), Pyruvate kinase (100191365), Pyruvate kinase (100191365), Pyruvate kinase (ZEAMMB73_Zm00001d026619), Pyruvate kinase (100193244), Pyruvate kinase, Pyruvate kinase (100193244), Pyruvate kinase (100193244)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Metal bindingi251MagnesiumBy similarity1
Metal bindingi302MagnesiumBy similarity1
Binding sitei302SubstrateBy similarity1
Metal bindingi329MagnesiumBy similarity1
Binding sitei329SubstrateBy similarity1
Active sitei354Proton acceptorBy similarity1
Binding sitei405SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1
2-phosphoglycerate dehydratase 1
Gene namesi
Name:ENO1
Synonyms:PGH1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi30060.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340731 – 446Enolase 1Add BLAST446

Proteomic databases

PaxDbiP26301.
PRIDEiP26301.
ProMEXiP26301.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi4577.GRMZM2G064302_P01.

Structurei

3D structure databases

ProteinModelPortaliP26301.
SMRiP26301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 384Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P26301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTITWVKA RQIFDSRGNP TVEVDVGLSD GSYARGAVPS GASTGIYEAL
60 70 80 90 100
ELRDGGSDYL GKGVLKAVSN VNNIIGPAIV GKDPTEQVEI DNFMVQQLDG
110 120 130 140 150
TSNEWGWCKQ KLGANAILAV SLAVCKAGAM VKKIPLYQHI ANLAGNKTLV
160 170 180 190 200
LPVPAFNVIN GGSHAGNKLA MQEFMILPTG ASSFKEAMKM GVEVYHNLKS
210 220 230 240 250
IIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKAAIEKA GYTGKVVIGM
260 270 280 290 300
DVAASEFFGE KDKTYDLNFK EENNDGSNKI SGDSLKDLYK SFVSEYPIES
310 320 330 340 350
IEDPFDQDDW STYAKLTDEI GQKVQIVGDD LLVTNPTRVA KAINEKTCNA
360 370 380 390 400
LLLKVNQIGS VTESIEAVRM SKRAGWGVMA SHRSGETEDT FIADLSVGLS
410 420 430 440
TGQIKTGAPC RSERLAKYNQ LLRIEEELGD AAVYAGAKFR APVEPY
Length:446
Mass (Da):48,064
Last modified:May 1, 1992 - v1
Checksum:i6266C48914F35198
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55981 mRNA. Translation: CAA39454.1.
PIRiS16257.
RefSeqiNP_001105896.1. NM_001112426.1.
UniGeneiZm.93820.

Genome annotation databases

GeneIDi732811.
KEGGizma:732811.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO1_MAIZE
AccessioniPrimary (citable) accession number: P26301
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 12, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families