Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enolase

Gene

PGH1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (PGH1)
  5. Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase, Pyruvate kinase
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163SubstrateBy similarity1
Binding sitei172SubstrateBy similarity1
Active sitei215Proton donorBy similarity1
Metal bindingi250MagnesiumBy similarity1
Metal bindingi300MagnesiumBy similarity1
Binding sitei300SubstrateBy similarity1
Metal bindingi327MagnesiumBy similarity1
Binding sitei327SubstrateBy similarity1
Active sitei352Proton acceptorBy similarity1
Binding sitei403SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:PGH1
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340721 – 444EnolaseAdd BLAST444

Proteomic databases

PaxDbiP26300.
PRIDEiP26300.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi4081.Solyc09g009020.2.1.

Structurei

3D structure databases

ProteinModelPortaliP26300.
SMRiP26300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 382Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
InParanoidiP26300.
KOiK01689.
OMAiYVEDPFH.
OrthoDBiEOG093608UK.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P26300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIKSIKAR QIFDSRGNPT VEVDVHISNG VFARAAVPSG ASTGIYEALE
60 70 80 90 100
LRDGGSDYLG KGVSKAVNNV NSIIGPALVG KDPTDQTGLD NFMVHQLDGT
110 120 130 140 150
QNEWGWCKEK LGANAILAVS LAVCKAGAAV RNVPLYKHIA DLAGNKKLVL
160 170 180 190 200
PVPAFNVING GSHAGNKLAM QEFMILPVGA ANFKEAMKMG CEVYHHLKAV
210 220 230 240 250
IKKKYGQDAT NVGDEGGFAP NIQENKEGLE LLKTAIEKAG YTGKVVIGMD
260 270 280 290 300
VAASEFYGKD KSYDLNFKEE SNDGSQKISG DQLKDLYKSF VSEYPIVSIE
310 320 330 340 350
DPFDQDDWET YAKLTAEIGE QVQIVGDDLL VTNPKRVAKA IAEKTCNALL
360 370 380 390 400
LKVNQIGSVT ESIEAVKMSK KAGWGVMTSH RSGETEDTFI ADLAVGLSTG
410 420 430 440
QIKTGAPCRS ERLAKYNQLL RIEEELGSEA VYAGASFRKP VEPY
Length:444
Mass (Da):47,798
Last modified:May 1, 1992 - v1
Checksum:i73C384181ED620A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58108 mRNA. Translation: CAA41115.1.
PIRiJQ1185.
RefSeqiNP_001234080.1. NM_001247151.2.
UniGeneiLes.3932.

Genome annotation databases

EnsemblPlantsiSolyc09g009020.2.1; Solyc09g009020.2.1; Solyc09g009020.2.
GeneIDi544068.
GrameneiSolyc09g009020.2.1; Solyc09g009020.2.1; Solyc09g009020.2.
KEGGisly:544068.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_SOLLC
AccessioniPrimary (citable) accession number: P26300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 7, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families