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Protein

Enolase

Gene

PGH1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (PGH1)
  5. Pyruvate kinase, Pyruvate kinase (101263236), Pyruvate kinase (101262183), Pyruvate kinase (101250010), Pyruvate kinase (101265671), Pyruvate kinase (101248036), Pyruvate kinase (101259587), Pyruvate kinase (101261160), Pyruvate kinase (101265599)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163SubstrateBy similarity1
Binding sitei172SubstrateBy similarity1
Active sitei215Proton donorBy similarity1
Metal bindingi250MagnesiumBy similarity1
Metal bindingi300MagnesiumBy similarity1
Binding sitei300SubstrateBy similarity1
Metal bindingi327MagnesiumBy similarity1
Binding sitei327SubstrateBy similarity1
Active sitei352Proton acceptorBy similarity1
Binding sitei403SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:PGH1
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340721 – 444EnolaseAdd BLAST444

Proteomic databases

PaxDbiP26300
PRIDEiP26300

Expressioni

Gene expression databases

ExpressionAtlasiP26300 baseline and differential

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi4081.Solyc09g009020.2.1

Structurei

3D structure databases

ProteinModelPortaliP26300
SMRiP26300
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 382Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA
InParanoidiP26300
KOiK01689
OMAiAEERACN
OrthoDBiEOG093608UK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P26300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATIKSIKAR QIFDSRGNPT VEVDVHISNG VFARAAVPSG ASTGIYEALE
60 70 80 90 100
LRDGGSDYLG KGVSKAVNNV NSIIGPALVG KDPTDQTGLD NFMVHQLDGT
110 120 130 140 150
QNEWGWCKEK LGANAILAVS LAVCKAGAAV RNVPLYKHIA DLAGNKKLVL
160 170 180 190 200
PVPAFNVING GSHAGNKLAM QEFMILPVGA ANFKEAMKMG CEVYHHLKAV
210 220 230 240 250
IKKKYGQDAT NVGDEGGFAP NIQENKEGLE LLKTAIEKAG YTGKVVIGMD
260 270 280 290 300
VAASEFYGKD KSYDLNFKEE SNDGSQKISG DQLKDLYKSF VSEYPIVSIE
310 320 330 340 350
DPFDQDDWET YAKLTAEIGE QVQIVGDDLL VTNPKRVAKA IAEKTCNALL
360 370 380 390 400
LKVNQIGSVT ESIEAVKMSK KAGWGVMTSH RSGETEDTFI ADLAVGLSTG
410 420 430 440
QIKTGAPCRS ERLAKYNQLL RIEEELGSEA VYAGASFRKP VEPY
Length:444
Mass (Da):47,798
Last modified:May 1, 1992 - v1
Checksum:i73C384181ED620A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58108 mRNA Translation: CAA41115.1
PIRiJQ1185
RefSeqiNP_001234080.1, NM_001247151.2
UniGeneiLes.3932

Genome annotation databases

EnsemblPlantsiSolyc09g009020.2.1; Solyc09g009020.2.1; Solyc09g009020.2
GeneIDi544068
GrameneiSolyc09g009020.2.1; Solyc09g009020.2.1; Solyc09g009020.2
KEGGisly:544068

Similar proteinsi

Entry informationi

Entry nameiENO_SOLLC
AccessioniPrimary (citable) accession number: P26300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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