P26298 (LDHD_LACPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: D-lactate dehydrogenase Short name=D-LDH EC=1.1.1.28 Alternative name(s): D-specific D-2-hydroxyacid dehydrogenase
Organism	Lactobacillus pentosus
Taxonomic identifier	1589 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Subunit structure	Homodimer.
Miscellaneous	Also active on D-glycerate.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Caution	Was originally (Ref.1) thought to originate from L.plantarum.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	D-lactate dehydrogenase		PRO_0000075956
Regions
	Nucleotide binding	155 – 156	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1
	Active site	264	1
	Active site	296	1	Proton donor

Sequences

	Sequence		Length	Mass (Da)
	P26298 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: FA63723C63B53EBE	FASTA	332	37,183
	10 20 30 40 50 60 MKIIAYAVRD DERPFFDTWM KENPDVEVKL VPELLTEDNV DLAKGFDGAD VYQQKDYTAE 70 80 90 100 110 120 VLNKLADEGV KNISLRNVGV DNLDVPTVKA RGLNISNVPA YSPNAIAELS VTQLMQLLRQ 130 140 150 160 170 180 TPMFNKKLAK QDFRWAPDIA KELNTMTVGV IGTGRIGRAA IDIFKGFGAK VIGYDVYRNA 190 200 210 220 230 240 ELEKEGMYVD TLDELYAQAD VITLHVPALK DNYHMLNADA FSKMKDGAYI LNFARGTLID 250 260 270 280 290 300 SEDLIKALDS GKVAGAALVT YEYETKIFNK DLEGQTIDDK VFMNLFNRDN VLITPHTAFY 310 320 330 TETAVHNMVH VSMNSNKQFI ETGKADTQVK FD

References

[1]	"D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum." Ohta T., Taguchi H. J. Biol. Chem. 266:12588-12594(1991) [PubMed: 1840590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8041 / DSM 20314 / JCM 1558 / NCDO 363 / NCIMB 8026.
[2]	"Insights into substrate binding by D-2-ketoacid dehydrogenases from the structure of Lactobacillus pentosus D-lactate dehydrogenase." Stoll V.S., Kimber M.S., Pai E.F. Structure 4:437-447(1996) [PubMed: 8740366] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90339 Genomic DNA. Translation: BAA14352.1.
PIR	A40885.
3D structure databases
ProteinModelPortal	P26298.
SMR	P26298. Positions 1-330.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_LACPE

Accession

Primary (citable) accession number: P26298

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 28, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program