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Protein

15-cis-phytoene desaturase

Gene

pds

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme converts phytoene into zeta-carotene via the intermediary of phytofluene by the symmetrical introduction of two double bonds at the C-11 and C-11' positions of phytoene. Also active with phytofluene and 1,2-epoxyphytoene as substrates.1 Publication

Catalytic activityi

15-cis-phytoene + 2 plastoquinone = 9,15,9'-tri-cis-zeta-carotene + 2 plastoquinol.1 Publication

Enzyme regulationi

Inhibited by the herbicide norflurazon in a non-competitive way.

Kineticsi

  1. KM=3.5 µM for phytoene1 Publication
  2. KM=14.3 mM for NADP1 Publication

    Pathwayi: lycopene biosynthesis

    This protein is involved in the pathway lycopene biosynthesis, which is part of Carotenoid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway lycopene biosynthesis and in Carotenoid biosynthesis.

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carotenoid biosynthesis, Herbicide resistance

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:SYNPCC7942_0970-MONOMER.
    SYNEL:SYNPCC7942_0970-MONOMER.
    SYNEL:SYNPCC7942_1983-MONOMER.
    UniPathwayiUPA00803.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    15-cis-phytoene desaturase (EC:1.3.5.5)
    Alternative name(s):
    Phytoene dehydrogenase
    Gene namesi
    Name:pds
    Synonyms:crtP
    Ordered Locus Names:Synpcc7942_1983
    OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
    Taxonomic identifieri1140 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    Proteomesi
    • UP000002717 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Chemistry

    ChEMBLiCHEMBL2366472.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 47447415-cis-phytoene desaturasePRO_0000067697Add
    BLAST

    Expressioni

    Inductioni

    Up-regulated by light.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi1140.Synpcc7942_1983.

    Structurei

    3D structure databases

    ProteinModelPortaliP26294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CVN. Bacteria.
    COG3349. LUCA.
    HOGENOMiHOG000150110.
    KOiK02293.
    OrthoDBiPOG091H0N45.

    Family and domain databases

    Gene3Di3.50.50.60. 2 hits.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR014102. Phytoene_desaturase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 2 hits.
    TIGRFAMsiTIGR02731. phytoene_desat. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P26294-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVAIAGAGL AGLSCAKYLA DAGHTPIVYE RRDVLGGKVA AWKDEDGDWY
    60 70 80 90 100
    ETGLHIFFGA YPNMLQLFKE LNIEDRLQWK SHSMIFNQPT KPGTYSRFDF
    110 120 130 140 150
    PDIPAPINGV AAILSNNDML TWEEKIKFGL GLLPAMIRGQ SYVEEMDQYS
    160 170 180 190 200
    WTEWLRKQNI PERVNDEVFI AMAKALNFID PDEISATVVL TALNRFLQEK
    210 220 230 240 250
    KGSMMAFLDG APPERLCQPI VEHVQARGGD VLLNAPLKEF VLNDDSSVQA
    260 270 280 290 300
    FRIAGIKGQE EQLIEADAYV SALPVDPLKL LLPDAWKAMP YFQQLDGLQG
    310 320 330 340 350
    VPVINIHLWF DRKLTDIDHL LFSRSPLLSV YADMSNTCRE YEDPDRSMLE
    360 370 380 390 400
    LVFAPAKDWI GRSDEDILAA TMAEIEKLFP QHFSGENPAR LRKYKIVKTP
    410 420 430 440 450
    LSVYKATPGR QQYRPDQASP IANFFLTGDY TMQRYLASME GAVLSGKLTA
    460 470
    QAIIARQDEL QRRSSGRPLA ASQA
    Length:474
    Mass (Da):53,296
    Last modified:May 1, 1992 - v1
    Checksum:i793EC60CE7F2A005
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti403 – 4031V → G in strain: NZF4; confers resistance to the herbicide norflurazon.

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X55289 Genomic DNA. Translation: CAA39004.1.
    CP000100 Genomic DNA. Translation: ABB58013.1.
    RefSeqiWP_011378266.1. NC_007604.1.

    Genome annotation databases

    EnsemblBacteriaiABB58013; ABB58013; Synpcc7942_1983.
    KEGGisyf:Synpcc7942_1983.
    PATRICi23789425. VBISynElo51371_2235.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X55289 Genomic DNA. Translation: CAA39004.1.
    CP000100 Genomic DNA. Translation: ABB58013.1.
    RefSeqiWP_011378266.1. NC_007604.1.

    3D structure databases

    ProteinModelPortaliP26294.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi1140.Synpcc7942_1983.

    Chemistry

    ChEMBLiCHEMBL2366472.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABB58013; ABB58013; Synpcc7942_1983.
    KEGGisyf:Synpcc7942_1983.
    PATRICi23789425. VBISynElo51371_2235.

    Phylogenomic databases

    eggNOGiENOG4105CVN. Bacteria.
    COG3349. LUCA.
    HOGENOMiHOG000150110.
    KOiK02293.
    OrthoDBiPOG091H0N45.

    Enzyme and pathway databases

    UniPathwayiUPA00803.
    BioCyciMetaCyc:SYNPCC7942_0970-MONOMER.
    SYNEL:SYNPCC7942_0970-MONOMER.
    SYNEL:SYNPCC7942_1983-MONOMER.

    Family and domain databases

    Gene3Di3.50.50.60. 2 hits.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR014102. Phytoene_desaturase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51905. SSF51905. 2 hits.
    TIGRFAMsiTIGR02731. phytoene_desat. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPDS_SYNE7
    AccessioniPrimary (citable) accession number: P26294
    Secondary accession number(s): Q31LQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: September 7, 2016
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can also use NAD+ and NADP+ as electron acceptor under anaerobic conditions.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.