Reviewed, UniProtKB/Swiss-Prot P26291 (UCRIA_PEA)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit, chloroplastic
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP
      Short name=ISP
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein

Gene names

Name:

petC

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus. The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: EC ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast By similarity
	Chain	51 – 230	180	Cytochrome b6-f complex iron-sulfur subunit, chloroplastic		PRO_0000030691
Regions
	Transmembrane	72 – 92	21	Potential
	Domain	115 – 213	99	Rieske
Sites
	Metal binding	157	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	159	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	175	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	178	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	162 ↔ 177		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26291-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: A60E42BC68048091	FASTA	230	24,243
	10 20 30 40 50 60 MSSTTLSPTT PSQLCSGKSG ISCPSIALLV KPTRTQMTGR GNKGMKITCQ ATSIPADRVP 70 80 90 100 110 120 DMSKRKTLNL LLLGALSLPT AGMLVPYGSF LVPPGSGSST GGTVAKDAVG NDVVATEWLK 130 140 150 160 170 180 THAPGDRTLT QGLKGDPTYL VVEKDRTLAT FAINAVCTHL GCVVPFNQAE NKFICPCHGS 190 200 210 220 230 QYNDQGRVVR GPAPLSLALA HCDVGVEDGK VVFVPWVETD FRTGDAPWWS

References

[1]	"Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts." Salter A.H., Newman B.J., Napier J.A., Gray J.C. Plant Mol. Biol. 20:569-574(1992) [PubMed: 1421160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63605 mRNA. Translation: CAA45151.1.
PIR	S26199.
3D structure databases
SMR	P26291. Positions 103-230.
ModBase	Search...
Proteomic databases
PRIDE	P26291.
Enzyme and pathway databases
BRENDA	1.10.99.1. 287.
Family and domain databases
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRIA_PEA

Accession

Primary (citable) accession number: P26291

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)