Reviewed, UniProtKB/Swiss-Prot P26285 (F262_BOVIN)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Alternative name(s):
    PFK-2/FBPase-2
    6PF-2-K/Fru-2,6-P2ASE heart-type isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

PFKFB2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Enzyme regulation	Phosphorylation results in the activation of the kinase activity.
Subunit structure	Homodimer.
Tissue specificity	Heart.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 531	530	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		PRO_0000179963
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
	Region	2 – 249	248	6-phosphofructo-2-kinase
	Region	250 – 531	282	Fructose-2,6-bisphosphatase
Sites
	Active site	129	1	Potential
	Active site	159	1	Potential
	Active site	258	1	Tele-phosphohistidine intermediate
	Active site	327	1	Potential
	Active site	392	1	Proton donor By similarity
	Binding site	103	1	Fructose-6-phosphate By similarity
	Binding site	194	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	467	1	Phosphoserine; by PKA Ref.3
	Modified residue	469	1	Phosphothreonine By similarity
	Modified residue	476	1	Phosphothreonine; by PKC Ref.3
	Modified residue	484	1	Phosphoserine By similarity
	Modified residue	494	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26285-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E5090BBFD10BC594	FASTA	531	60,811
	10 20 30 40 50 60 MSGNPASSSE QNNNSYETKA SLRISEKKCS WASYMTNSPT LIVMIGLPAR GKTYVSKKLT 70 80 90 100 110 120 RYLNWIGVPT KVFNLGVYRR QAVKSYKSYD FFRHDNEEAM KIRKQCALVA LKDVKAYLTE 130 140 150 160 170 180 ESGQIAVFDA TNTTRERRDL ILNFAEENSF KVFFVESVCD DPDVIAANIL EVKVSSPDYP 190 200 210 220 230 240 ERNRENVMDD FLKRIECYKV TYQPLDPDSH DKDLSFIKVI NVGQRFLVNK VQDYIQSKIV 250 260 270 280 290 300 YYLMNIHVHP RTIYLCRHGE SEFNLLGKIG GDSGLSVRGK QFAQALRKFL EEQEIADLKV 310 320 330 340 350 360 WTSQLKRTIQ TAESLGVTYE QWKILNEIDA GVCEEMTYAE IQEQYPDEFA LRDEEKYLYR 370 380 390 400 410 420 YPGGESYQDL VQRLEPVIME LERQGNVLVI SHQAVMRCLL AYFLDKGADE LPYLRCPLHT 430 440 450 460 470 480 IFKLTPVAYG CKVETIKLNV EAVNTHRDKP TNNFPKSQTP VRMRRNSFTP LSSSNTIRRP 490 500 510 520 530 RNYSVGSRPL QPLSPLRALD TQEGADQPKT QAETSRAAHR LPSPAPPTSP S

References

[1]	"Bovine heart fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase: complete amino acid sequence and localization of phosphorylation sites." Sakata J., Uyeda K. Proc. Natl. Acad. Sci. U.S.A. 87:4951-4955(1990) [PubMed: 2164212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Bovine heart fructose 6-P,2-kinase:fructose 2,6-bisphosphatase mRNA and gene structure." Tsuchiya Y., Uyeda K. Arch. Biochem. Biophys. 310:467-474(1994) [PubMed: 8179334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"Phosphorylation of myocardial fructose-6-phosphate,2-kinase: fructose-2,6-bisphosphatase by cAMP-dependent protein kinase and protein kinase C. Activation by phosphorylation and amino acid sequences of the phosphorylation sites." Kitamura K., Kangawa K., Matsuo H., Uyeda K. J. Biol. Chem. 263:16796-16801(1988) [PubMed: 2846551] [Abstract] Cited for: PROTEIN SEQUENCE OF 464-480, PHOSPHORYLATION AT SER-467 AND THR-476. Tissue: Heart.
[4]	"Catalytic site of rat liver and bovine heart fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase. Identification of fructose 6-phosphate binding site." Kitamura K., Uyeda K., Hartman F.C., Kangawa K., Matsuo H. J. Biol. Chem. 264:6344-6348(1989) [PubMed: 2539378] [Abstract] Cited for: PROTEIN SEQUENCE OF 104-112; 189-204 AND 416-432.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M34241 mRNA. Translation: AAA30523.1. S70453 mRNA. Translation: AAB30689.2.
IPI	IPI00709752.
PIR	A31780. S44388.
RefSeq	NP_777237.3.
UniGene	Bt.3949
3D structure databases
HSSP	HSSP built from PDB template 1C80 based on UniProtKB P07953.
ModBase	Search...
Protein-protein interaction databases
STRING	P26285.
Genome annotation databases
Ensembl	ENSBTAT00000002753; ENSBTAP00000002753; ENSBTAG00000002126; Bos taurus. [Genome view]
GeneID	287019.
KEGG	bta:287019.
Organism-specific databases
CTD	287019.
Phylogenomic databases
HOVERGEN	P26285.
OMA	HPRTIYL.
Enzyme and pathway databases
BRENDA	2.7.1.105. 251. 3.1.3.46. 251.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase_AC. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F262_BOVIN

Accession

Primary (citable) accession number: P26285

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)