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P26281

- HPPK_ECOLI

UniProt

P26281 - HPPK_ECOLI

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Protein

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

Gene
folK, b0142, JW0138
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.

Pathwayi

GO - Molecular functioni

  1. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Source: EcoCyc
  2. ATP binding Source: UniProtKB-KW
  3. kinase activity Source: UniProtKB-KW
  4. magnesium ion binding Source: EcoCyc

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Folate biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER.
ECOL316407:JW0138-MONOMER.
MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER.
SABIO-RKP26281.
UniPathwayiUPA00077; UER00155.

Names & Taxonomyi

Protein namesi
Recommended name:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC:2.7.6.3)
Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name:
PPPK
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name:
HPPK
Gene namesi
Name:folK
Ordered Locus Names:b0142, JW0138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11374. folK.

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 1591582-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinasePRO_0000168249Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP26281.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP26281. 2 interactions.
STRINGi511145.b0142.

Structurei

Secondary structure

1
159
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108
Beta strandi12 – 143
Helixi15 – 2713
Beta strandi32 – 376
Beta strandi41 – 433
Beta strandi46 – 483
Beta strandi49 – 513
Beta strandi54 – 6310
Helixi67 – 8014
Turni82 – 865
Beta strandi87 – 904
Helixi91 – 933
Beta strandi96 – 1027
Beta strandi107 – 1115
Beta strandi113 – 1153
Helixi119 – 1213
Helixi123 – 13210
Beta strandi139 – 1413
Helixi144 – 1518

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DY3X-ray2.00A2-159[»]
1EQ0NMR-A2-159[»]
1EQMX-ray1.50A2-159[»]
1EX8X-ray1.85A2-159[»]
1F9HX-ray1.50A2-159[»]
1G4CX-ray1.65A/B2-159[»]
1HKAX-ray1.50A2-159[»]
1HQ2X-ray1.25A2-159[»]
1IM6X-ray1.74A2-159[»]
1KBRX-ray1.55A2-159[»]
1Q0NX-ray1.25A2-159[»]
1RAOX-ray1.56A2-159[»]
1RB0X-ray1.35A2-159[»]
1RTZX-ray1.33A2-159[»]
1RU1X-ray1.40A/B2-159[»]
1RU2X-ray1.48A2-159[»]
1TMJX-ray1.45A2-159[»]
1TMMX-ray1.25A/B2-159[»]
2F63NMR-A2-159[»]
2F65NMR-A2-159[»]
3HCXX-ray1.75A2-159[»]
3HD1X-ray1.30A2-159[»]
3HD2X-ray1.10A2-159[»]
3HSDX-ray1.65A/B2-159[»]
3HSGX-ray1.14A2-159[»]
3HSJX-ray1.18A2-159[»]
3HSZX-ray1.40A2-159[»]
3HT0X-ray1.40A2-159[»]
3ILIX-ray1.45A2-159[»]
3ILJX-ray1.65A2-159[»]
3ILLX-ray1.73A2-159[»]
3ILOX-ray1.10A2-159[»]
3IP0X-ray0.89A2-159[»]
3KUEX-ray1.54A2-159[»]
3KUGX-ray2.00A2-159[»]
3KUHX-ray1.35A2-159[»]
3UD5X-ray2.00A2-159[»]
3UDEX-ray1.88A2-159[»]
3UDVX-ray1.88A2-159[»]
4F7VX-ray1.73A2-159[»]
4M5GX-ray1.31A1-159[»]
4M5HX-ray1.11A1-159[»]
4M5IX-ray1.08A1-159[»]
4M5JX-ray1.70A1-159[»]
4M5KX-ray1.30A1-159[»]
4M5LX-ray1.09A1-159[»]
4M5MX-ray1.12A1-159[»]
4M5NX-ray2.00A/B1-159[»]
ProteinModelPortaliP26281.
SMRiP26281. Positions 2-159.

Miscellaneous databases

EvolutionaryTraceiP26281.

Family & Domainsi

Sequence similaritiesi

Belongs to the HPPK family.

Phylogenomic databases

eggNOGiCOG0801.
HOGENOMiHOG000217741.
KOiK00950.
OMAiFDELSKW.
OrthoDBiEOG6XHC8G.
PhylomeDBiP26281.

Family and domain databases

Gene3Di3.30.70.560. 1 hit.
InterProiIPR000550. Hppk.
[Graphical view]
PfamiPF01288. HPPK. 1 hit.
[Graphical view]
SUPFAMiSSF55083. SSF55083. 1 hit.
TIGRFAMsiTIGR01498. folK. 1 hit.
PROSITEiPS00794. HPPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26281-1 [UniParc]FASTAAdd to Basket

« Hide

MTVAYIAIGS NLASPLEQVN AALKALGDIP ESHILTVSSF YRTPPLGPQD    50
QPDYLNAAVA LETSLAPEEL LNHTQRIELQ QGRVRKAERW GPRTLDLDIM 100
LFGNEVINTE RLTVPHYDMK NRGFMLWPLF EIAPELVFPD GEMLRQILHT 150
RAFDKLNKW 159
Length:159
Mass (Da):18,079
Last modified:January 23, 2007 - v3
Checksum:i67301CD634D0A174
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06495 Genomic DNA. Translation: AAB53446.1.
M20574 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73253.1.
AP009048 Genomic DNA. Translation: BAB96719.1.
PIRiA43325.
RefSeqiNP_414684.1. NC_000913.3.
YP_488445.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73253; AAC73253; b0142.
BAB96719; BAB96719; BAB96719.
GeneIDi12934036.
948792.
KEGGiecj:Y75_p0139.
eco:b0142.
PATRICi32115391. VBIEscCol129921_0147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L06495 Genomic DNA. Translation: AAB53446.1 .
M20574 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73253.1 .
AP009048 Genomic DNA. Translation: BAB96719.1 .
PIRi A43325.
RefSeqi NP_414684.1. NC_000913.3.
YP_488445.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DY3 X-ray 2.00 A 2-159 [» ]
1EQ0 NMR - A 2-159 [» ]
1EQM X-ray 1.50 A 2-159 [» ]
1EX8 X-ray 1.85 A 2-159 [» ]
1F9H X-ray 1.50 A 2-159 [» ]
1G4C X-ray 1.65 A/B 2-159 [» ]
1HKA X-ray 1.50 A 2-159 [» ]
1HQ2 X-ray 1.25 A 2-159 [» ]
1IM6 X-ray 1.74 A 2-159 [» ]
1KBR X-ray 1.55 A 2-159 [» ]
1Q0N X-ray 1.25 A 2-159 [» ]
1RAO X-ray 1.56 A 2-159 [» ]
1RB0 X-ray 1.35 A 2-159 [» ]
1RTZ X-ray 1.33 A 2-159 [» ]
1RU1 X-ray 1.40 A/B 2-159 [» ]
1RU2 X-ray 1.48 A 2-159 [» ]
1TMJ X-ray 1.45 A 2-159 [» ]
1TMM X-ray 1.25 A/B 2-159 [» ]
2F63 NMR - A 2-159 [» ]
2F65 NMR - A 2-159 [» ]
3HCX X-ray 1.75 A 2-159 [» ]
3HD1 X-ray 1.30 A 2-159 [» ]
3HD2 X-ray 1.10 A 2-159 [» ]
3HSD X-ray 1.65 A/B 2-159 [» ]
3HSG X-ray 1.14 A 2-159 [» ]
3HSJ X-ray 1.18 A 2-159 [» ]
3HSZ X-ray 1.40 A 2-159 [» ]
3HT0 X-ray 1.40 A 2-159 [» ]
3ILI X-ray 1.45 A 2-159 [» ]
3ILJ X-ray 1.65 A 2-159 [» ]
3ILL X-ray 1.73 A 2-159 [» ]
3ILO X-ray 1.10 A 2-159 [» ]
3IP0 X-ray 0.89 A 2-159 [» ]
3KUE X-ray 1.54 A 2-159 [» ]
3KUG X-ray 2.00 A 2-159 [» ]
3KUH X-ray 1.35 A 2-159 [» ]
3UD5 X-ray 2.00 A 2-159 [» ]
3UDE X-ray 1.88 A 2-159 [» ]
3UDV X-ray 1.88 A 2-159 [» ]
4F7V X-ray 1.73 A 2-159 [» ]
4M5G X-ray 1.31 A 1-159 [» ]
4M5H X-ray 1.11 A 1-159 [» ]
4M5I X-ray 1.08 A 1-159 [» ]
4M5J X-ray 1.70 A 1-159 [» ]
4M5K X-ray 1.30 A 1-159 [» ]
4M5L X-ray 1.09 A 1-159 [» ]
4M5M X-ray 1.12 A 1-159 [» ]
4M5N X-ray 2.00 A/B 1-159 [» ]
ProteinModelPortali P26281.
SMRi P26281. Positions 2-159.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P26281. 2 interactions.
STRINGi 511145.b0142.

Chemistry

DrugBanki DB00131. Adenosine monophosphate.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73253 ; AAC73253 ; b0142 .
BAB96719 ; BAB96719 ; BAB96719 .
GeneIDi 12934036.
948792.
KEGGi ecj:Y75_p0139.
eco:b0142.
PATRICi 32115391. VBIEscCol129921_0147.

Organism-specific databases

EchoBASEi EB1348.
EcoGenei EG11374. folK.

Phylogenomic databases

eggNOGi COG0801.
HOGENOMi HOG000217741.
KOi K00950.
OMAi FDELSKW.
OrthoDBi EOG6XHC8G.
PhylomeDBi P26281.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00155 .
BioCyci EcoCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER.
ECOL316407:JW0138-MONOMER.
MetaCyc:H2PTERIDINEPYROPHOSPHOKIN-MONOMER.
SABIO-RK P26281.

Miscellaneous databases

EvolutionaryTracei P26281.
PROi P26281.

Gene expression databases

Genevestigatori P26281.

Family and domain databases

Gene3Di 3.30.70.560. 1 hit.
InterProi IPR000550. Hppk.
[Graphical view ]
Pfami PF01288. HPPK. 1 hit.
[Graphical view ]
SUPFAMi SSF55083. SSF55083. 1 hit.
TIGRFAMsi TIGR01498. folK. 1 hit.
PROSITEi PS00794. HPPK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase."
    Talarico T.L., Ray P.H., Dev I.K., Merrill B.M., Dallas W.S.
    J. Bacteriol. 174:5971-5977(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100."
    Talarico T.L., Dev I.K., Dallas W.S., Ferone R., Ray P.H.
    J. Bacteriol. 173:7029-7032(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-29.
  6. "Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control."
    Liu J., Parkinson J.S.
    J. Bacteriol. 171:1254-1261(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
  7. "Crystal structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, a potential target for the development of novel antimicrobial agents."
    Xiao B., Shi G., Chen X., Yan H., Ji X.
    Structure 7:489-496(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
  8. "2.0 A X-ray structure of the ternary complex of 7,8-dihydro-6-hydroxymethylpterinpyrophosphokinase from Escherichia coli with ATP and a substrate analogue."
    Stammers D.K., Achari A., Somers D.O., Bryant P.K., Rosemond J., Scott D.L., Champness J.N.
    FEBS Lett. 456:49-53(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiHPPK_ECOLI
AccessioniPrimary (citable) accession number: P26281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is heat stable.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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