Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

26S proteasome non-ATPase regulatory subunit 7

Gene

Rpn8

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication

GO - Biological processi

  • neurogenesis Source: FlyBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiM67.973.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 7
Alternative name(s):
26S proteasome regulatory subunit RPN8
26S proteasome regulatory subunit S12
Proteasome subunit p39B
Proteasome subunit p40
Protein Mov34
Gene namesi
Name:Rpn8
Synonyms:Mov34
ORF Names:CG3416
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0002787. Rpn8.

Subcellular locationi

GO - Cellular componenti

  • dendrite Source: FlyBase
  • proteasome complex Source: FlyBase
  • proteasome regulatory particle Source: FlyBase
  • proteasome regulatory particle, lid subcomplex Source: FlyBase

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002139452 – 33826S proteasome non-ATPase regulatory subunit 7Add BLAST337

Proteomic databases

PaxDbiP26270.
PRIDEiP26270.

Expressioni

Gene expression databases

BgeeiFBgn0002787.
ExpressionAtlasiP26270. differential.
GenevisibleiP26270. DM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Rpn9Q7KMP83EBI-253612,EBI-151692

Protein-protein interaction databases

BioGridi63468. 28 interactors.
DIPiDIP-22847N.
IntActiP26270. 17 interactors.
MINTiMINT-819762.
STRINGi7227.FBpp0303270.

Structurei

3D structure databases

ProteinModelPortaliP26270.
SMRiP26270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 121MPNAdd BLAST116

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi289 – 338Glu/Lys-richAdd BLAST50

Sequence similaritiesi

Belongs to the peptidase M67A family.Curated

Phylogenomic databases

eggNOGiKOG1556. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00530000063075.
InParanoidiP26270.
KOiK03038.
OrthoDBiEOG091G0DX6.
PhylomeDBiP26270.

Family and domain databases

CDDicd08062. MPN_RPN7_8. 1 hit.
InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
IPR033858. MPN_RPN7_8.
IPR024969. Rpn11/EIF3F_C.
PANTHERiPTHR10540:SF13. PTHR10540:SF13. 1 hit.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSQEVSVNK VIVHPLVLLS VVDHFNRMGK IGNQKRVVGV LLGCWRSKGV
60 70 80 90 100
LDVSNSFAVP FDEDDKDKSV WFLDHDYLEN MYGMFKKVNA RERVVGWYHT
110 120 130 140 150
GPKLHQNDIA INELVRRYCP NSVLVIIDAK PKDLGLPTEA YISVEEVHDD
160 170 180 190 200
GSPTSKTFEH VPSEIGAEEA EEVGVEHLLR DIKDTTVGSL SQKITNQLMG
210 220 230 240 250
LKGLNAQLRD IKQYLQRVGD SKMPINHQIV YQLQDIFNLL PDITNDQFTG
260 270 280 290 300
TMYVKTNDQM LVVYLASMVR SIIALHNLIN NKLANRDAEE GKSDSKEAKE
310 320 330
KNKDSKDKDN KETKDKDGKK AEEKADKGKD EGGKGSRK
Length:338
Mass (Da):38,089
Last modified:January 23, 2007 - v6
Checksum:i1074BBB94268B2D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56S → T in AAA28695 (PubMed:2209467).Curated1
Sequence conflicti63E → V in AAA28695 (PubMed:2209467).Curated1
Sequence conflicti121 – 146NSVLV…ISVEE → TPCWSSSTPSPRIWACPQRR TYRWRK in AAA28695 (PubMed:2209467).CuratedAdd BLAST26
Sequence conflicti167A → P in AAA28695 (PubMed:2209467).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64643 mRNA. Translation: AAA28695.1.
AE013599 Genomic DNA. Translation: AAF47199.1.
AY089283 mRNA. Translation: AAL90021.1.
RefSeqiNP_001261168.1. NM_001274239.1.
NP_523845.2. NM_079121.3.
UniGeneiDm.38517.
Dm.770.

Genome annotation databases

EnsemblMetazoaiFBtr0072290; FBpp0072197; FBgn0002787.
FBtr0332921; FBpp0305141; FBgn0002787.
GeneIDi37894.
KEGGidme:Dmel_CG3416.
UCSCiCG3416-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64643 mRNA. Translation: AAA28695.1.
AE013599 Genomic DNA. Translation: AAF47199.1.
AY089283 mRNA. Translation: AAL90021.1.
RefSeqiNP_001261168.1. NM_001274239.1.
NP_523845.2. NM_079121.3.
UniGeneiDm.38517.
Dm.770.

3D structure databases

ProteinModelPortaliP26270.
SMRiP26270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63468. 28 interactors.
DIPiDIP-22847N.
IntActiP26270. 17 interactors.
MINTiMINT-819762.
STRINGi7227.FBpp0303270.

Protein family/group databases

MEROPSiM67.973.

Proteomic databases

PaxDbiP26270.
PRIDEiP26270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072290; FBpp0072197; FBgn0002787.
FBtr0332921; FBpp0305141; FBgn0002787.
GeneIDi37894.
KEGGidme:Dmel_CG3416.
UCSCiCG3416-RA. d. melanogaster.

Organism-specific databases

CTDi37894.
FlyBaseiFBgn0002787. Rpn8.

Phylogenomic databases

eggNOGiKOG1556. Eukaryota.
COG1310. LUCA.
GeneTreeiENSGT00530000063075.
InParanoidiP26270.
KOiK03038.
OrthoDBiEOG091G0DX6.
PhylomeDBiP26270.

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii37894.
PROiPR:P26270.

Gene expression databases

BgeeiFBgn0002787.
ExpressionAtlasiP26270. differential.
GenevisibleiP26270. DM.

Family and domain databases

CDDicd08062. MPN_RPN7_8. 1 hit.
InterProiView protein in InterPro
IPR000555. JAMM/MPN+_dom.
IPR033858. MPN_RPN7_8.
IPR024969. Rpn11/EIF3F_C.
PANTHERiPTHR10540:SF13. PTHR10540:SF13. 1 hit.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
PF13012. MitMem_reg. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD7_DROME
AccessioniPrimary (citable) accession number: P26270
Secondary accession number(s): Q9W178
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 146 of the entry and version 6 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.