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P26265

- CPDB_SALTY

UniProt

P26265 - CPDB_SALTY

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Protein

2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase

Gene

cpdB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'-cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate.

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate.
A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Divalent cations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Divalent metal cation 1By similarity
Metal bindingi33 – 331Divalent metal cation 1By similarity
Metal bindingi76 – 761Divalent metal cation 1By similarity
Metal bindingi76 – 761Divalent metal cation 2By similarity
Metal bindingi116 – 1161Divalent metal cation 2By similarity
Metal bindingi225 – 2251Divalent metal cation 2By similarity
Metal bindingi257 – 2571Divalent metal cation 2By similarity
Metal bindingi259 – 2591Divalent metal cation 1By similarity
Binding sitei440 – 4401SubstrateBy similarity

GO - Molecular functioni

  1. 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: UniProtKB-EC
  2. 3'-nucleotidase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1810-MONOMER.
SENT99287:GCTI-4435-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC:3.1.3.6, EC:3.1.4.16)
Gene namesi
Name:cpdB
Ordered Locus Names:STM4403
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 6476282',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidasePRO_0000000036Add
BLAST

Proteomic databases

PaxDbiP26265.
PRIDEiP26265.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4403.

Structurei

3D structure databases

ProteinModelPortaliP26265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5507Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0737.
HOGENOMiHOG000248800.
KOiK01119.
OMAiEKAQYPM.
OrthoDBiEOG696BW0.
PhylomeDBiP26265.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR006294. Cyc_nuc_PDE_nucleotidase.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01390. CycNucDiestase. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26265 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKFSATLLA TLIAASVNAA TVDLRIMETT DLHSNMMDFD YYKDTATEKF
60 70 80 90 100
GLVRTASLIH AARNEVKNSV LVDNGDLIQG SPLGDYMAAK GLKDGDVHPV
110 120 130 140 150
YKALNTLDYA VGNLGNHEFN YGLDYLHNAL AGAKFPYVNA NIIDVKTQKP
160 170 180 190 200
LFTPYLIKET SVIDKDGNPQ TLKIGYIGFV PPQIMIWDKA NLSGKVTVND
210 220 230 240 250
ITETARKYVP EMREKGADIV VVIAHSGLSA DPYHSMAENS VYYLSEVPGV
260 270 280 290 300
DAIMFGHAHA VFPGKDFADI KGADIAKGTL NGIPAVMPGM WGDHLGVVDL
310 320 330 340 350
VLNNDSGKWQ VTQAKAEARP IYDAAAKKSL AAEDSKLVGI LKADHDATRE
360 370 380 390 400
FVSKPIGKSA DNMYSYLALV QDDPTVQVVN NAQKAYVEHF IQGDPDLAKL
410 420 430 440 450
PVLSAAAPFK VGGRKNDPAS FVEVEKGQLT FRNAADLYLY PNTLVVVKAS
460 470 480 490 500
GKEVKEWLEC SAGQFNQIDI HSNKPQSLIN WDGFRTYNFD VIDGVNYQID
510 520 530 540 550
VSQPARYDGE CQMVNPQAER IKNLTFNGKP VDPNATFLVA TNNYRAYGGK
560 570 580 590 600
FAGTGDSHIA FASPDENRAV LAAWIGAESK RAGEIHPAAD NNWRLAPIHS
610 620 630 640
DTALDIRFET SPGDKAAAFI KAKGQYPMKK VAVDDIGFAI YQVDLSK
Length:647
Mass (Da):70,517
Last modified:December 13, 2001 - v2
Checksum:iE9F7FE7D6681DB34
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511G → A in CAA37956. (PubMed:2172762)Curated
Sequence conflicti84 – 896GDYMAA → RLYGG in CAA37956. (PubMed:2172762)Curated
Sequence conflicti96 – 983DVH → GIQ in CAA37956. (PubMed:2172762)Curated
Sequence conflicti133 – 1331A → G in CAA37956. (PubMed:2172762)Curated
Sequence conflicti174 – 1741I → N in CAA37956. (PubMed:2172762)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23223.1.
X54009 Genomic DNA. Translation: CAA37956.1.
PIRiS11915.
RefSeqiNP_463264.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23223; AAL23223; STM4403.
GeneIDi1255929.
KEGGistm:STM4403.
PATRICi32387729. VBISalEnt20916_4628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23223.1 .
X54009 Genomic DNA. Translation: CAA37956.1 .
PIRi S11915.
RefSeqi NP_463264.1. NC_003197.1.

3D structure databases

ProteinModelPortali P26265.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM4403.

Proteomic databases

PaxDbi P26265.
PRIDEi P26265.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL23223 ; AAL23223 ; STM4403 .
GeneIDi 1255929.
KEGGi stm:STM4403.
PATRICi 32387729. VBISalEnt20916_4628.

Phylogenomic databases

eggNOGi COG0737.
HOGENOMi HOG000248800.
KOi K01119.
OMAi EKAQYPM.
OrthoDBi EOG696BW0.
PhylomeDBi P26265.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-1810-MONOMER.
SENT99287:GCTI-4435-MONOMER.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR006294. Cyc_nuc_PDE_nucleotidase.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsi TIGR01390. CycNucDiestase. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Transcription and regulation of the cpdB gene in Escherichia coli K12 and Salmonella typhimurium LT2: evidence for modulation of constitutive promoters by cyclic AMP-CRP complex."
    Liu J., Beacham I.R.
    Mol. Gen. Genet. 222:161-165(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251.
    Strain: LT2.

Entry informationi

Entry nameiCPDB_SALTY
AccessioniPrimary (citable) accession number: P26265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 13, 2001
Last modified: October 29, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two kinetically distinguishable active sites for the two substrates (2',3'-cyclic nucleotides and 3'-nucleotides) have been identified.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3