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P26265

- CPDB_SALTY

UniProt

P26265 - CPDB_SALTY

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Protein
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
Gene
cpdB, STM4403
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'-cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate.

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate.
A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Divalent cations By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Divalent metal cation 1 By similarity
Metal bindingi33 – 331Divalent metal cation 1 By similarity
Metal bindingi76 – 761Divalent metal cation 1 By similarity
Metal bindingi76 – 761Divalent metal cation 2 By similarity
Metal bindingi116 – 1161Divalent metal cation 2 By similarity
Metal bindingi225 – 2251Divalent metal cation 2 By similarity
Metal bindingi257 – 2571Divalent metal cation 2 By similarity
Metal bindingi259 – 2591Divalent metal cation 1 By similarity
Binding sitei440 – 4401Substrate By similarity

GO - Molecular functioni

  1. 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: UniProtKB-EC
  2. 3'-nucleotidase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1810-MONOMER.
SENT99287:GCTI-4435-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC:3.1.3.6, EC:3.1.4.16)
Gene namesi
Name:cpdB
Ordered Locus Names:STM4403
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 6476282',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
PRO_0000000036Add
BLAST

Proteomic databases

PaxDbiP26265.
PRIDEiP26265.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4403.

Structurei

3D structure databases

ProteinModelPortaliP26265.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5507Substrate binding By similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0737.
HOGENOMiHOG000248800.
KOiK01119.
OMAiEKAQYPM.
OrthoDBiEOG696BW0.
PhylomeDBiP26265.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR006294. Cyc_nuc_PDE_nucleotidase.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01390. CycNucDiestase. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26265-1 [UniParc]FASTAAdd to Basket

« Hide

MIKFSATLLA TLIAASVNAA TVDLRIMETT DLHSNMMDFD YYKDTATEKF    50
GLVRTASLIH AARNEVKNSV LVDNGDLIQG SPLGDYMAAK GLKDGDVHPV 100
YKALNTLDYA VGNLGNHEFN YGLDYLHNAL AGAKFPYVNA NIIDVKTQKP 150
LFTPYLIKET SVIDKDGNPQ TLKIGYIGFV PPQIMIWDKA NLSGKVTVND 200
ITETARKYVP EMREKGADIV VVIAHSGLSA DPYHSMAENS VYYLSEVPGV 250
DAIMFGHAHA VFPGKDFADI KGADIAKGTL NGIPAVMPGM WGDHLGVVDL 300
VLNNDSGKWQ VTQAKAEARP IYDAAAKKSL AAEDSKLVGI LKADHDATRE 350
FVSKPIGKSA DNMYSYLALV QDDPTVQVVN NAQKAYVEHF IQGDPDLAKL 400
PVLSAAAPFK VGGRKNDPAS FVEVEKGQLT FRNAADLYLY PNTLVVVKAS 450
GKEVKEWLEC SAGQFNQIDI HSNKPQSLIN WDGFRTYNFD VIDGVNYQID 500
VSQPARYDGE CQMVNPQAER IKNLTFNGKP VDPNATFLVA TNNYRAYGGK 550
FAGTGDSHIA FASPDENRAV LAAWIGAESK RAGEIHPAAD NNWRLAPIHS 600
DTALDIRFET SPGDKAAAFI KAKGQYPMKK VAVDDIGFAI YQVDLSK 647
Length:647
Mass (Da):70,517
Last modified:December 13, 2001 - v2
Checksum:iE9F7FE7D6681DB34
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511G → A in CAA37956. 1 Publication
Sequence conflicti84 – 896GDYMAA → RLYGG in CAA37956. 1 Publication
Sequence conflicti96 – 983DVH → GIQ in CAA37956. 1 Publication
Sequence conflicti133 – 1331A → G in CAA37956. 1 Publication
Sequence conflicti174 – 1741I → N in CAA37956. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23223.1.
X54009 Genomic DNA. Translation: CAA37956.1.
PIRiS11915.
RefSeqiNP_463264.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23223; AAL23223; STM4403.
GeneIDi1255929.
KEGGistm:STM4403.
PATRICi32387729. VBISalEnt20916_4628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23223.1 .
X54009 Genomic DNA. Translation: CAA37956.1 .
PIRi S11915.
RefSeqi NP_463264.1. NC_003197.1.

3D structure databases

ProteinModelPortali P26265.
ModBasei Search...

Protein-protein interaction databases

STRINGi 99287.STM4403.

Proteomic databases

PaxDbi P26265.
PRIDEi P26265.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL23223 ; AAL23223 ; STM4403 .
GeneIDi 1255929.
KEGGi stm:STM4403.
PATRICi 32387729. VBISalEnt20916_4628.

Phylogenomic databases

eggNOGi COG0737.
HOGENOMi HOG000248800.
KOi K01119.
OMAi EKAQYPM.
OrthoDBi EOG696BW0.
PhylomeDBi P26265.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-1810-MONOMER.
SENT99287:GCTI-4435-MONOMER.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR006294. Cyc_nuc_PDE_nucleotidase.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
TIGRFAMsi TIGR01390. CycNucDiestase. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Transcription and regulation of the cpdB gene in Escherichia coli K12 and Salmonella typhimurium LT2: evidence for modulation of constitutive promoters by cyclic AMP-CRP complex."
    Liu J., Beacham I.R.
    Mol. Gen. Genet. 222:161-165(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251.
    Strain: LT2.

Entry informationi

Entry nameiCPDB_SALTY
AccessioniPrimary (citable) accession number: P26265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 13, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two kinetically distinguishable active sites for the two substrates (2',3'-cyclic nucleotides and 3'-nucleotides) have been identified.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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