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P26265

- CPDB_SALTY

UniProt

P26265 - CPDB_SALTY

Protein

2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase

Gene

cpdB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (13 Dec 2001)
      Previous versions | rss
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    Functioni

    This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'-cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate.

    Catalytic activityi

    Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate.
    A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.

    Cofactori

    Divalent cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi31 – 311Divalent metal cation 1By similarity
    Metal bindingi33 – 331Divalent metal cation 1By similarity
    Metal bindingi76 – 761Divalent metal cation 1By similarity
    Metal bindingi76 – 761Divalent metal cation 2By similarity
    Metal bindingi116 – 1161Divalent metal cation 2By similarity
    Metal bindingi225 – 2251Divalent metal cation 2By similarity
    Metal bindingi257 – 2571Divalent metal cation 2By similarity
    Metal bindingi259 – 2591Divalent metal cation 1By similarity
    Binding sitei440 – 4401SubstrateBy similarity

    GO - Molecular functioni

    1. 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity Source: UniProtKB-EC
    2. 3'-nucleotidase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. nucleotide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-1810-MONOMER.
    SENT99287:GCTI-4435-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (EC:3.1.3.6, EC:3.1.4.16)
    Gene namesi
    Name:cpdB
    Ordered Locus Names:STM4403
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 6476282',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidasePRO_0000000036Add
    BLAST

    Proteomic databases

    PaxDbiP26265.
    PRIDEiP26265.

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM4403.

    Structurei

    3D structure databases

    ProteinModelPortaliP26265.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni544 – 5507Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0737.
    HOGENOMiHOG000248800.
    KOiK01119.
    OMAiEKAQYPM.
    OrthoDBiEOG696BW0.
    PhylomeDBiP26265.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProiIPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR006294. Cyc_nuc_PDE_nucleotidase.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PANTHERiPTHR11575. PTHR11575. 1 hit.
    PfamiPF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR01607. APYRASEFAMLY.
    SUPFAMiSSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    TIGRFAMsiTIGR01390. CycNucDiestase. 1 hit.
    PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26265-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKFSATLLA TLIAASVNAA TVDLRIMETT DLHSNMMDFD YYKDTATEKF    50
    GLVRTASLIH AARNEVKNSV LVDNGDLIQG SPLGDYMAAK GLKDGDVHPV 100
    YKALNTLDYA VGNLGNHEFN YGLDYLHNAL AGAKFPYVNA NIIDVKTQKP 150
    LFTPYLIKET SVIDKDGNPQ TLKIGYIGFV PPQIMIWDKA NLSGKVTVND 200
    ITETARKYVP EMREKGADIV VVIAHSGLSA DPYHSMAENS VYYLSEVPGV 250
    DAIMFGHAHA VFPGKDFADI KGADIAKGTL NGIPAVMPGM WGDHLGVVDL 300
    VLNNDSGKWQ VTQAKAEARP IYDAAAKKSL AAEDSKLVGI LKADHDATRE 350
    FVSKPIGKSA DNMYSYLALV QDDPTVQVVN NAQKAYVEHF IQGDPDLAKL 400
    PVLSAAAPFK VGGRKNDPAS FVEVEKGQLT FRNAADLYLY PNTLVVVKAS 450
    GKEVKEWLEC SAGQFNQIDI HSNKPQSLIN WDGFRTYNFD VIDGVNYQID 500
    VSQPARYDGE CQMVNPQAER IKNLTFNGKP VDPNATFLVA TNNYRAYGGK 550
    FAGTGDSHIA FASPDENRAV LAAWIGAESK RAGEIHPAAD NNWRLAPIHS 600
    DTALDIRFET SPGDKAAAFI KAKGQYPMKK VAVDDIGFAI YQVDLSK 647
    Length:647
    Mass (Da):70,517
    Last modified:December 13, 2001 - v2
    Checksum:iE9F7FE7D6681DB34
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511G → A in CAA37956. (PubMed:2172762)Curated
    Sequence conflicti84 – 896GDYMAA → RLYGG in CAA37956. (PubMed:2172762)Curated
    Sequence conflicti96 – 983DVH → GIQ in CAA37956. (PubMed:2172762)Curated
    Sequence conflicti133 – 1331A → G in CAA37956. (PubMed:2172762)Curated
    Sequence conflicti174 – 1741I → N in CAA37956. (PubMed:2172762)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23223.1.
    X54009 Genomic DNA. Translation: CAA37956.1.
    PIRiS11915.
    RefSeqiNP_463264.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL23223; AAL23223; STM4403.
    GeneIDi1255929.
    KEGGistm:STM4403.
    PATRICi32387729. VBISalEnt20916_4628.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23223.1 .
    X54009 Genomic DNA. Translation: CAA37956.1 .
    PIRi S11915.
    RefSeqi NP_463264.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P26265.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM4403.

    Proteomic databases

    PaxDbi P26265.
    PRIDEi P26265.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL23223 ; AAL23223 ; STM4403 .
    GeneIDi 1255929.
    KEGGi stm:STM4403.
    PATRICi 32387729. VBISalEnt20916_4628.

    Phylogenomic databases

    eggNOGi COG0737.
    HOGENOMi HOG000248800.
    KOi K01119.
    OMAi EKAQYPM.
    OrthoDBi EOG696BW0.
    PhylomeDBi P26265.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-1810-MONOMER.
    SENT99287:GCTI-4435-MONOMER.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProi IPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR006294. Cyc_nuc_PDE_nucleotidase.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    PANTHERi PTHR11575. PTHR11575. 1 hit.
    Pfami PF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR01607. APYRASEFAMLY.
    SUPFAMi SSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    TIGRFAMsi TIGR01390. CycNucDiestase. 1 hit.
    PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "Transcription and regulation of the cpdB gene in Escherichia coli K12 and Salmonella typhimurium LT2: evidence for modulation of constitutive promoters by cyclic AMP-CRP complex."
      Liu J., Beacham I.R.
      Mol. Gen. Genet. 222:161-165(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-251.
      Strain: LT2.

    Entry informationi

    Entry nameiCPDB_SALTY
    AccessioniPrimary (citable) accession number: P26265
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: December 13, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two kinetically distinguishable active sites for the two substrates (2',3'-cyclic nucleotides and 3'-nucleotides) have been identified.

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3