Plasma kallikrein precursor - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (?) or add your own

P26262 (KLKB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Plasma kallikrein
EC=3.4.21.34

Alternative name(s):
Fletcher factor
Kininogenin
Plasma prekallikrein

Gene names

Name:	Klkb1
Synonyms:	Klk3, Pk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Catalytic activity	Cleaves selectively Arg-\|-Xaa and Lys-\|-Xaa bonds, including Lys-\|-Arg and Arg-\|-Ser bonds in (human) kininogen to release bradykinin.
Enzyme regulation	Inhibited by SERPINA5 By similarity.
Subunit structure	Forms heterodimer with SERPINA5. The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Plasma kallikrein subfamily. Contains 4 apple domains. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Blood coagulation Fibrinolysis Inflammatory response
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW fibrinolysis Inferred from electronic annotation. Source: UniProtKB-KW inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Chain

20 – 390

371

Plasma kallikrein heavy chain

PRO_0000028023

Chain

391 – 638

248

Plasma kallikrein light chain

PRO_0000028024

Regions

Domain

21 – 104

Apple 1

Domain

111 – 194

Apple 2

Domain

201 – 284

Apple 3

Domain

292 – 375

Apple 4

Domain

391 – 626

236

Peptidase S1

Sites

Active site

434

Charge relay system

Active site

483

Charge relay system

Active site

578

Charge relay system

Amino acid modifications

Glycosylation

127

N-linked (GlcNAc...) Ref.3 Ref.4

Glycosylation

215

N-linked (GlcNAc...) Potential

Glycosylation

308

N-linked (GlcNAc...) Potential

Glycosylation

396

N-linked (GlcNAc...) Ref.4

Glycosylation

494

N-linked (GlcNAc...) Ref.4

Disulfide bond

21 ↔ 104

By similarity

Disulfide bond

47 ↔ 77

By similarity

Disulfide bond

51 ↔ 57

By similarity

Disulfide bond

111 ↔ 194

By similarity

Disulfide bond

137 ↔ 166

By similarity

Disulfide bond

141 ↔ 147

By similarity

Disulfide bond

201 ↔ 284

By similarity

Disulfide bond

227 ↔ 256

By similarity

Disulfide bond

231 ↔ 237

By similarity

Disulfide bond

292 ↔ 375

By similarity

Disulfide bond

318 ↔ 347

By similarity

Disulfide bond

322 ↔ 328

By similarity

Disulfide bond

340 ↔ 345

By similarity

Disulfide bond

383 ↔ 503

By similarity

Disulfide bond

419 ↔ 435

By similarity

Disulfide bond

517 ↔ 584

By similarity

Disulfide bond

548 ↔ 563

By similarity

Disulfide bond

574 ↔ 602

By similarity

Experimental info

Sequence conflict

603

A → G in AAA63393. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P26262 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: CC27C93F4B57C599
Show »

FASTA

638

71,383

        10         20         30         40         50         60 
MILFNRVGYF VSLFATVSCG CMTQLYKNTF FRGGDLAAIY TPDAQYCQKM CTFHPRCLLF 

        70         80         90        100        110        120 
SFLAVTPPKE TNKRFGCFMK ESITGTLPRI HRTGAISGHS LKQCGHQISA CHRDIYKGLD 

       130        140        150        160        170        180 
MRGSNFNISK TDNIEECQKL CTNNFHCQFF TYATSAFYRP EYRKKCLLKH SASGTPTSIK 

       190        200        210        220        230        240 
SADNLVSGFS LKSCALSEIG CPMDIFQHSA FADLNVSQVI TPDAFVCRTI CTFHPNCLFF 

       250        260        270        280        290        300 
TFYTNEWETE SQRNVCFLKT SKSGRPSPPI PQENAISGYS LLTCRKTRPE PCHSKIYSGV 

       310        320        330        340        350        360 
DFEGEELNVT FVQGADVCQE TCTKTIRCQF FIYSLLPQDC KEEGCKCSLR LSTDGSPTRI 

       370        380        390        400        410        420 
TYGMQGSSGY SLRLCKLVDS PDCTTKINAR IVGGTNASLG EWPWQVSLQV KLVSQTHLCG 

       430        440        450        460        470        480 
GSIIGRQWVL TAAHCFDGIP YPDVWRIYGG ILSLSEITKE TPSSRIKELI IHQEYKVSEG 

       490        500        510        520        530        540 
NYDIALIKLQ TPLNYTEFQK PICLPSKADT NTIYTNCWVT GWGYTKEQGE TQNILQKATI 

       550        560        570        580        590        600 
PLVPNEECQK KYRDYVINKQ MICAGYKEGG TDACKGDSGG PLVCKHSGRW QLVGITSWGE 

       610        620        630 
GCARKDQPGV YTKVSEYMDW ILEKTQSSDV RALETSSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse plasma kallikrein: cDNA structure, enzyme characterization, and comparison of protein and mRNA levels among species." Seidah N.G., Sawyer N., Hamelin J., Mion P., Beaubien G., Brachpapa L., Rochemont J., Mbikay M., Chretien M. DNA Cell Biol. 9:737-748(1990) [PubMed: 2264928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: BALB/c. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[3]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-127, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-127; ASN-396 AND ASN-494, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58588 mRNA. Translation: AAA63393.1. BC026555 mRNA. Translation: AAH26555.1.
IPI	IPI00113057.
PIR	KQMSPL. A36557.
RefSeq	NP_032481.2. NM_008455.2.
UniGene	Mm.482691.
3D structure databases
ProteinModelPortal	P26262.
SMR	P26262. Positions 20-385, 391-628.
ModBase	Search...
Protein-protein interaction databases
STRING	P26262.
Protein family/group databases
MEROPS	S01.212.
Proteomic databases
PRIDE	P26262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026907; ENSMUSP00000026907; ENSMUSG00000031640. ENSMUST00000116473; ENSMUSP00000112174; ENSMUSG00000031640.
GeneID	16621.
KEGG	mmu:16621.
Organism-specific databases
CTD	3818.
MGI	MGI:102849. Klkb1.
Phylogenomic databases
eggNOG	roNOG08709.
HOGENOM	HBG281927.
HOVERGEN	HBG000399.
InParanoid	P26262.
OrthoDB	EOG4FFD18.
Enzyme and pathway databases
BRENDA	3.4.21.34. 3474.
Gene expression databases
ArrayExpress	P26262.
Bgee	P26262.
Genevestigator	P26262.
GermOnline	ENSMUSG00000031640. Mus musculus.
Family and domain databases
InterPro	IPR000177. Apple. IPR003014. PAN-1_domain. IPR003609. Pan_app. IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01324.
Pfam	PF00024. PAN_1. 4 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00005. APPLEDOMAIN. PR00722. CHYMOTRYPSIN.
SMART	SM00223. APPLE. 4 hits. SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS00495. APPLE. 4 hits. PS50948. PAN. 4 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290227.
SOURCE	Search...

Entry information

Entry name

KLKB1_MOUSE

Accession

Primary (citable) accession number: P26262
Secondary accession number(s): Q8R0P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents