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Protein

Plasma kallikrein

Gene

Klkb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Catalytic activityi

Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei434Charge relay system1
Active sitei483Charge relay system1
Active sitei578Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Blood coagulation, Fibrinolysis, Hemostasis, Inflammatory response

Enzyme and pathway databases

BRENDAi3.4.21.34. 3474.
ReactomeiR-MMU-140837. Intrinsic Pathway of Fibrin Clot Formation.
R-MMU-1592389. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.212.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma kallikrein (EC:3.4.21.34)
Alternative name(s):
Fletcher factor
Kininogenin
Plasma prekallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Klkb1
Synonyms:Klk3, Pk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102849. Klkb1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250359.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002802320 – 390Plasma kallikrein heavy chainAdd BLAST371
ChainiPRO_0000028024391 – 638Plasma kallikrein light chainAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 104By similarity
Disulfide bondi47 ↔ 77By similarity
Disulfide bondi51 ↔ 57By similarity
Disulfide bondi111 ↔ 194By similarity
Glycosylationi127N-linked (GlcNAc...)2 Publications1
Disulfide bondi137 ↔ 166By similarity
Disulfide bondi141 ↔ 147By similarity
Disulfide bondi201 ↔ 284By similarity
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi227 ↔ 256By similarity
Disulfide bondi231 ↔ 237By similarity
Disulfide bondi292 ↔ 375By similarity
Glycosylationi308N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi318 ↔ 347By similarity
Disulfide bondi322 ↔ 328By similarity
Disulfide bondi340 ↔ 345By similarity
Disulfide bondi383 ↔ 503By similarity
Glycosylationi396N-linked (GlcNAc...)1 Publication1
Disulfide bondi419 ↔ 435By similarity
Glycosylationi494N-linked (GlcNAc...)1 Publication1
Disulfide bondi517 ↔ 584By similarity
Disulfide bondi548 ↔ 563By similarity
Disulfide bondi574 ↔ 602By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP26262.
PaxDbiP26262.
PeptideAtlasiP26262.
PRIDEiP26262.

PTM databases

iPTMnetiP26262.
PhosphoSitePlusiP26262.
SwissPalmiP26262.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031640.
GenevisibleiP26262. MM.

Interactioni

Subunit structurei

Forms a heterodimer with SERPINA5. The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112174.

Structurei

3D structure databases

ProteinModelPortaliP26262.
SMRiP26262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 104Apple 1PROSITE-ProRule annotationAdd BLAST84
Domaini111 – 194Apple 2PROSITE-ProRule annotationAdd BLAST84
Domaini201 – 284Apple 3PROSITE-ProRule annotationAdd BLAST84
Domaini292 – 375Apple 4PROSITE-ProRule annotationAdd BLAST84
Domaini391 – 626Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasma kallikrein subfamily.PROSITE-ProRule annotation
Contains 4 apple domains.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112467.
HOVERGENiHBG000399.
InParanoidiP26262.
KOiK01324.
OMAiVCFLKTS.
OrthoDBiEOG091G0AH5.
TreeFamiTF343687.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR000177. Apple.
IPR003609. Pan_app.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00024. PAN_1. 4 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00005. APPLEDOMAIN.
PR00722. CHYMOTRYPSIN.
SMARTiSM00223. APPLE. 4 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00495. APPLE. 4 hits.
PS50948. PAN. 4 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILFNRVGYF VSLFATVSCG CMTQLYKNTF FRGGDLAAIY TPDAQYCQKM
60 70 80 90 100
CTFHPRCLLF SFLAVTPPKE TNKRFGCFMK ESITGTLPRI HRTGAISGHS
110 120 130 140 150
LKQCGHQISA CHRDIYKGLD MRGSNFNISK TDNIEECQKL CTNNFHCQFF
160 170 180 190 200
TYATSAFYRP EYRKKCLLKH SASGTPTSIK SADNLVSGFS LKSCALSEIG
210 220 230 240 250
CPMDIFQHSA FADLNVSQVI TPDAFVCRTI CTFHPNCLFF TFYTNEWETE
260 270 280 290 300
SQRNVCFLKT SKSGRPSPPI PQENAISGYS LLTCRKTRPE PCHSKIYSGV
310 320 330 340 350
DFEGEELNVT FVQGADVCQE TCTKTIRCQF FIYSLLPQDC KEEGCKCSLR
360 370 380 390 400
LSTDGSPTRI TYGMQGSSGY SLRLCKLVDS PDCTTKINAR IVGGTNASLG
410 420 430 440 450
EWPWQVSLQV KLVSQTHLCG GSIIGRQWVL TAAHCFDGIP YPDVWRIYGG
460 470 480 490 500
ILSLSEITKE TPSSRIKELI IHQEYKVSEG NYDIALIKLQ TPLNYTEFQK
510 520 530 540 550
PICLPSKADT NTIYTNCWVT GWGYTKEQGE TQNILQKATI PLVPNEECQK
560 570 580 590 600
KYRDYVINKQ MICAGYKEGG TDACKGDSGG PLVCKHSGRW QLVGITSWGE
610 620 630
GCARKDQPGV YTKVSEYMDW ILEKTQSSDV RALETSSA
Length:638
Mass (Da):71,383
Last modified:July 27, 2011 - v2
Checksum:iCC27C93F4B57C599
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti603A → G in AAA63393 (PubMed:2264928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58588 mRNA. Translation: AAA63393.1.
BC026555 mRNA. Translation: AAH26555.1.
CCDSiCCDS22275.1.
PIRiA36557. KQMSPL.
RefSeqiNP_032481.2. NM_008455.3.
UniGeneiMm.482691.

Genome annotation databases

EnsembliENSMUST00000026907; ENSMUSP00000026907; ENSMUSG00000109764.
GeneIDi16621.
KEGGimmu:16621.
UCSCiuc009lot.4. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58588 mRNA. Translation: AAA63393.1.
BC026555 mRNA. Translation: AAH26555.1.
CCDSiCCDS22275.1.
PIRiA36557. KQMSPL.
RefSeqiNP_032481.2. NM_008455.3.
UniGeneiMm.482691.

3D structure databases

ProteinModelPortaliP26262.
SMRiP26262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112174.

Chemistry databases

ChEMBLiCHEMBL1250359.

Protein family/group databases

MEROPSiS01.212.

PTM databases

iPTMnetiP26262.
PhosphoSitePlusiP26262.
SwissPalmiP26262.

Proteomic databases

MaxQBiP26262.
PaxDbiP26262.
PeptideAtlasiP26262.
PRIDEiP26262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026907; ENSMUSP00000026907; ENSMUSG00000109764.
GeneIDi16621.
KEGGimmu:16621.
UCSCiuc009lot.4. mouse.

Organism-specific databases

CTDi3818.
MGIiMGI:102849. Klkb1.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112467.
HOVERGENiHBG000399.
InParanoidiP26262.
KOiK01324.
OMAiVCFLKTS.
OrthoDBiEOG091G0AH5.
TreeFamiTF343687.

Enzyme and pathway databases

BRENDAi3.4.21.34. 3474.
ReactomeiR-MMU-140837. Intrinsic Pathway of Fibrin Clot Formation.
R-MMU-1592389. Activation of Matrix Metalloproteinases.

Miscellaneous databases

PROiP26262.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031640.
GenevisibleiP26262. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR000177. Apple.
IPR003609. Pan_app.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00024. PAN_1. 4 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00005. APPLEDOMAIN.
PR00722. CHYMOTRYPSIN.
SMARTiSM00223. APPLE. 4 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00495. APPLE. 4 hits.
PS50948. PAN. 4 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLKB1_MOUSE
AccessioniPrimary (citable) accession number: P26262
Secondary accession number(s): Q8R0P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.