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Protein

Plasma kallikrein

Gene

Klkb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Catalytic activityi

Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei434Charge relay system1
Active sitei483Charge relay system1
Active sitei578Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processBlood coagulation, Fibrinolysis, Hemostasis, Inflammatory response

Enzyme and pathway databases

BRENDAi3.4.21.34 3474
ReactomeiR-MMU-140837 Intrinsic Pathway of Fibrin Clot Formation
R-MMU-1592389 Activation of Matrix Metalloproteinases

Protein family/group databases

MEROPSiS01.212

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma kallikrein (EC:3.4.21.34)
Alternative name(s):
Fletcher factor
Kininogenin
Plasma prekallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Klkb1
Synonyms:Klk3, Pk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102849 Klkb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1250359

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002802320 – 390Plasma kallikrein heavy chainAdd BLAST371
ChainiPRO_0000028024391 – 638Plasma kallikrein light chainAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 104By similarity
Disulfide bondi47 ↔ 77By similarity
Disulfide bondi51 ↔ 57By similarity
Disulfide bondi111 ↔ 194By similarity
Glycosylationi127N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi137 ↔ 166By similarity
Disulfide bondi141 ↔ 147By similarity
Disulfide bondi201 ↔ 284By similarity
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi227 ↔ 256By similarity
Disulfide bondi231 ↔ 237By similarity
Disulfide bondi292 ↔ 375By similarity
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi318 ↔ 347By similarity
Disulfide bondi322 ↔ 328By similarity
Disulfide bondi340 ↔ 345By similarity
Disulfide bondi383 ↔ 503By similarity
Glycosylationi396N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi419 ↔ 435By similarity
Glycosylationi494N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi517 ↔ 584By similarity
Disulfide bondi548 ↔ 563By similarity
Disulfide bondi574 ↔ 602By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP26262
PaxDbiP26262
PeptideAtlasiP26262
PRIDEiP26262

PTM databases

iPTMnetiP26262
PhosphoSitePlusiP26262
SwissPalmiP26262

Expressioni

Gene expression databases

BgeeiENSMUSG00000031640
ExpressionAtlasiP26262 baseline and differential
GenevisibleiP26262 MM

Interactioni

Subunit structurei

Forms a heterodimer with SERPINA5. The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112174

Structurei

Secondary structure

1638
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi405 – 423Combined sources19
Beta strandi425 – 431Combined sources7
Helixi433 – 436Combined sources4
Helixi442 – 444Combined sources3
Beta strandi445 – 448Combined sources4
Helixi454 – 456Combined sources3
Beta strandi466 – 471Combined sources6
Beta strandi477 – 479Combined sources3
Beta strandi485 – 491Combined sources7
Helixi507 – 509Combined sources3
Beta strandi517 – 523Combined sources7
Beta strandi525 – 528Combined sources4
Beta strandi536 – 539Combined sources4
Helixi545 – 551Combined sources7
Beta strandi553 – 555Combined sources3
Beta strandi561 – 564Combined sources4
Beta strandi581 – 586Combined sources6
Beta strandi589 – 596Combined sources8
Beta strandi609 – 613Combined sources5
Helixi614 – 617Combined sources4
Helixi618 – 624Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GVTX-ray2.61A/B391-638[»]
ProteinModelPortaliP26262
SMRiP26262
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 104Apple 1PROSITE-ProRule annotationAdd BLAST84
Domaini111 – 194Apple 2PROSITE-ProRule annotationAdd BLAST84
Domaini201 – 284Apple 3PROSITE-ProRule annotationAdd BLAST84
Domaini292 – 375Apple 4PROSITE-ProRule annotationAdd BLAST84
Domaini391 – 626Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasma kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118962
HOGENOMiHOG000112467
HOVERGENiHBG000399
InParanoidiP26262
KOiK01324
OrthoDBiEOG091G0AH5
TreeFamiTF343687

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR000177 Apple
IPR003609 Pan_app
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR034813 Plasma_kallikrein
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR44459:SF2 PTHR44459:SF2, 1 hit
PfamiView protein in Pfam
PF00024 PAN_1, 4 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00005 APPLEDOMAIN
PR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00223 APPLE, 4 hits
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS00495 APPLE, 4 hits
PS50948 PAN, 4 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILFNRVGYF VSLFATVSCG CMTQLYKNTF FRGGDLAAIY TPDAQYCQKM
60 70 80 90 100
CTFHPRCLLF SFLAVTPPKE TNKRFGCFMK ESITGTLPRI HRTGAISGHS
110 120 130 140 150
LKQCGHQISA CHRDIYKGLD MRGSNFNISK TDNIEECQKL CTNNFHCQFF
160 170 180 190 200
TYATSAFYRP EYRKKCLLKH SASGTPTSIK SADNLVSGFS LKSCALSEIG
210 220 230 240 250
CPMDIFQHSA FADLNVSQVI TPDAFVCRTI CTFHPNCLFF TFYTNEWETE
260 270 280 290 300
SQRNVCFLKT SKSGRPSPPI PQENAISGYS LLTCRKTRPE PCHSKIYSGV
310 320 330 340 350
DFEGEELNVT FVQGADVCQE TCTKTIRCQF FIYSLLPQDC KEEGCKCSLR
360 370 380 390 400
LSTDGSPTRI TYGMQGSSGY SLRLCKLVDS PDCTTKINAR IVGGTNASLG
410 420 430 440 450
EWPWQVSLQV KLVSQTHLCG GSIIGRQWVL TAAHCFDGIP YPDVWRIYGG
460 470 480 490 500
ILSLSEITKE TPSSRIKELI IHQEYKVSEG NYDIALIKLQ TPLNYTEFQK
510 520 530 540 550
PICLPSKADT NTIYTNCWVT GWGYTKEQGE TQNILQKATI PLVPNEECQK
560 570 580 590 600
KYRDYVINKQ MICAGYKEGG TDACKGDSGG PLVCKHSGRW QLVGITSWGE
610 620 630
GCARKDQPGV YTKVSEYMDW ILEKTQSSDV RALETSSA
Length:638
Mass (Da):71,383
Last modified:July 27, 2011 - v2
Checksum:iCC27C93F4B57C599
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti603A → G in AAA63393 (PubMed:2264928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58588 mRNA Translation: AAA63393.1
BC026555 mRNA Translation: AAH26555.1
CCDSiCCDS22275.1
PIRiA36557 KQMSPL
RefSeqiNP_032481.2, NM_008455.3
UniGeneiMm.482691

Genome annotation databases

EnsembliENSMUST00000026907; ENSMUSP00000026907; ENSMUSG00000109764
GeneIDi16621
KEGGimmu:16621
UCSCiuc009lot.4 mouse

Similar proteinsi

Entry informationi

Entry nameiKLKB1_MOUSE
AccessioniPrimary (citable) accession number: P26262
Secondary accession number(s): Q8R0P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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