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Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Temperature dependencei

Thermostable up to 70 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei389 – 3891Proton donorBy similarity
Active sitei462 – 4621NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3780-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiThermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Taxonomic identifieri33950 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Beta-galactosidasePRO_0000057679Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP26257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIIPINNN WYFKADYEEG YEKVDDLRSF ENVNLPHTNI ELPYNYFDEK
60 70 80 90 100
MYQIKSCYKY PLHISEKYRD KVIYIHFEGV MAYAQVYLNG LYIGEHKGGY
110 120 130 140 150
TPFDIRIDEV YDWKKELNML TVVVDSTERS DIPPKGGQID YLTYGGIYRE
160 170 180 190 200
VSLGIYDDVF IKNIKVETHG IYDNEKSLNL IVHLENLNHQ SGNVKFKVKI
210 220 230 240 250
NDKNGKEVFY KEFNTYLDAV KDVYSFNIEN LKDIKLWDVD NPNLYEIKVG
260 270 280 290 300
MKINNFSDEY DNKFGFREAV FKPDGFYLNG RKLKLRGLNR HQSYPYVGYA
310 320 330 340 350
MPRRVQEKDA EILKNELHLN IVRTSHYPQS KHFLNKCDEL GLLVFEEIPG
360 370 380 390 400
WQYIGNSEWK KVAEQNLREM ITRDWNHPSI ILWGVRINES QDDDAFYKNM
410 420 430 440 450
NKIAHEIDPT RQTGGVRYIT NSSFLEDVYT FNDFIHDGIN KPLRKQQEVT
460 470 480 490 500
GLEHNVPYLV TEYNGHMYPT KRFDNEERQM EHCLRHLRIQ NASYLDDSIS
510 520 530 540 550
GAIGWCAFDY NTHKDFGSGD RICYHGVMDM FRLPKFASYV YKSQVSPDIE
560 570 580 590 600
PILEPVTFWA RGERSIGGVI PLIIFTNCDY IELQYGNKTK IDNIYPNRDA
610 620 630 640 650
YKGIPYPPII IDYDIVKPEM IGAWGMVWED LTLKGFYKGN KVIERKFSRE
660 670 680 690 700
PIPTYLYVVP DDTILSATQK DATRIVVKIL DQYGNLLPFI NEVIKIEIEG
710
PAKLQGPNEV ALIGGA
Length:716
Mass (Da):83,781
Last modified:May 1, 1992 - v1
Checksum:iB4665DE22C6F5F40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57579 Genomic DNA. Translation: AAA23249.1.
PIRiJU0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57579 Genomic DNA. Translation: AAA23249.1.
PIRiJU0275.

3D structure databases

ProteinModelPortaliP26257.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3780-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and analysis of the beta-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1."
    Burchhardt G., Bahl H.
    Gene 106:13-19(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
    Strain: DSM 3896 / EM1.

Entry informationi

Entry nameiBGAL_THETU
AccessioniPrimary (citable) accession number: P26257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.