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P26257

- BGAL_THETU

UniProt

P26257 - BGAL_THETU

Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Temperature dependencei

    Thermostable up to 70 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei389 – 3891Proton donorBy similarity
    Active sitei462 – 4621NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3780-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:lacZ
    OrganismiThermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
    Taxonomic identifieri33950 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Beta-galactosidasePRO_0000057679Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP26257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SUPFAMiSSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKIIPINNN WYFKADYEEG YEKVDDLRSF ENVNLPHTNI ELPYNYFDEK    50
    MYQIKSCYKY PLHISEKYRD KVIYIHFEGV MAYAQVYLNG LYIGEHKGGY 100
    TPFDIRIDEV YDWKKELNML TVVVDSTERS DIPPKGGQID YLTYGGIYRE 150
    VSLGIYDDVF IKNIKVETHG IYDNEKSLNL IVHLENLNHQ SGNVKFKVKI 200
    NDKNGKEVFY KEFNTYLDAV KDVYSFNIEN LKDIKLWDVD NPNLYEIKVG 250
    MKINNFSDEY DNKFGFREAV FKPDGFYLNG RKLKLRGLNR HQSYPYVGYA 300
    MPRRVQEKDA EILKNELHLN IVRTSHYPQS KHFLNKCDEL GLLVFEEIPG 350
    WQYIGNSEWK KVAEQNLREM ITRDWNHPSI ILWGVRINES QDDDAFYKNM 400
    NKIAHEIDPT RQTGGVRYIT NSSFLEDVYT FNDFIHDGIN KPLRKQQEVT 450
    GLEHNVPYLV TEYNGHMYPT KRFDNEERQM EHCLRHLRIQ NASYLDDSIS 500
    GAIGWCAFDY NTHKDFGSGD RICYHGVMDM FRLPKFASYV YKSQVSPDIE 550
    PILEPVTFWA RGERSIGGVI PLIIFTNCDY IELQYGNKTK IDNIYPNRDA 600
    YKGIPYPPII IDYDIVKPEM IGAWGMVWED LTLKGFYKGN KVIERKFSRE 650
    PIPTYLYVVP DDTILSATQK DATRIVVKIL DQYGNLLPFI NEVIKIEIEG 700
    PAKLQGPNEV ALIGGA 716
    Length:716
    Mass (Da):83,781
    Last modified:May 1, 1992 - v1
    Checksum:iB4665DE22C6F5F40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57579 Genomic DNA. Translation: AAA23249.1.
    PIRiJU0275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57579 Genomic DNA. Translation: AAA23249.1 .
    PIRi JU0275.

    3D structure databases

    ProteinModelPortali P26257.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-3780-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SUPFAMi SSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and analysis of the beta-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1."
      Burchhardt G., Bahl H.
      Gene 106:13-19(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
      Strain: DSM 3896 / EM1.

    Entry informationi

    Entry nameiBGAL_THETU
    AccessioniPrimary (citable) accession number: P26257
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3