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P26257

- BGAL_THETU

UniProt

P26257 - BGAL_THETU

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Protein
Beta-galactosidase
Gene
lacZ
Organism
Thermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Temperature dependencei

Thermostable up to 70 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei389 – 3891Proton donor By similarity
Active sitei462 – 4621Nucleophile By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3780-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiThermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Taxonomic identifieri33950 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Beta-galactosidase
PRO_0000057679Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP26257.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26257-1 [UniParc]FASTAAdd to Basket

« Hide

MRKIIPINNN WYFKADYEEG YEKVDDLRSF ENVNLPHTNI ELPYNYFDEK    50
MYQIKSCYKY PLHISEKYRD KVIYIHFEGV MAYAQVYLNG LYIGEHKGGY 100
TPFDIRIDEV YDWKKELNML TVVVDSTERS DIPPKGGQID YLTYGGIYRE 150
VSLGIYDDVF IKNIKVETHG IYDNEKSLNL IVHLENLNHQ SGNVKFKVKI 200
NDKNGKEVFY KEFNTYLDAV KDVYSFNIEN LKDIKLWDVD NPNLYEIKVG 250
MKINNFSDEY DNKFGFREAV FKPDGFYLNG RKLKLRGLNR HQSYPYVGYA 300
MPRRVQEKDA EILKNELHLN IVRTSHYPQS KHFLNKCDEL GLLVFEEIPG 350
WQYIGNSEWK KVAEQNLREM ITRDWNHPSI ILWGVRINES QDDDAFYKNM 400
NKIAHEIDPT RQTGGVRYIT NSSFLEDVYT FNDFIHDGIN KPLRKQQEVT 450
GLEHNVPYLV TEYNGHMYPT KRFDNEERQM EHCLRHLRIQ NASYLDDSIS 500
GAIGWCAFDY NTHKDFGSGD RICYHGVMDM FRLPKFASYV YKSQVSPDIE 550
PILEPVTFWA RGERSIGGVI PLIIFTNCDY IELQYGNKTK IDNIYPNRDA 600
YKGIPYPPII IDYDIVKPEM IGAWGMVWED LTLKGFYKGN KVIERKFSRE 650
PIPTYLYVVP DDTILSATQK DATRIVVKIL DQYGNLLPFI NEVIKIEIEG 700
PAKLQGPNEV ALIGGA 716
Length:716
Mass (Da):83,781
Last modified:May 1, 1992 - v1
Checksum:iB4665DE22C6F5F40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57579 Genomic DNA. Translation: AAA23249.1.
PIRiJU0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57579 Genomic DNA. Translation: AAA23249.1 .
PIRi JU0275.

3D structure databases

ProteinModelPortali P26257.
ModBasei Search...

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-3780-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SUPFAMi SSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and analysis of the beta-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1."
    Burchhardt G., Bahl H.
    Gene 106:13-19(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
    Strain: DSM 3896 / EM1.

Entry informationi

Entry nameiBGAL_THETU
AccessioniPrimary (citable) accession number: P26257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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