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P26257 (BGAL_THETU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
OrganismThermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
Taxonomic identifier33950 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family III. Incertae SedisThermoanaerobacterium

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Biophysicochemical properties

Temperature dependence:

Thermostable up to 70 degrees Celsius.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 716716Beta-galactosidase
PRO_0000057679

Sites

Active site3891Proton donor By similarity
Active site4621Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P26257 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: B4665DE22C6F5F40

FASTA71683,781
        10         20         30         40         50         60 
MRKIIPINNN WYFKADYEEG YEKVDDLRSF ENVNLPHTNI ELPYNYFDEK MYQIKSCYKY 

        70         80         90        100        110        120 
PLHISEKYRD KVIYIHFEGV MAYAQVYLNG LYIGEHKGGY TPFDIRIDEV YDWKKELNML 

       130        140        150        160        170        180 
TVVVDSTERS DIPPKGGQID YLTYGGIYRE VSLGIYDDVF IKNIKVETHG IYDNEKSLNL 

       190        200        210        220        230        240 
IVHLENLNHQ SGNVKFKVKI NDKNGKEVFY KEFNTYLDAV KDVYSFNIEN LKDIKLWDVD 

       250        260        270        280        290        300 
NPNLYEIKVG MKINNFSDEY DNKFGFREAV FKPDGFYLNG RKLKLRGLNR HQSYPYVGYA 

       310        320        330        340        350        360 
MPRRVQEKDA EILKNELHLN IVRTSHYPQS KHFLNKCDEL GLLVFEEIPG WQYIGNSEWK 

       370        380        390        400        410        420 
KVAEQNLREM ITRDWNHPSI ILWGVRINES QDDDAFYKNM NKIAHEIDPT RQTGGVRYIT 

       430        440        450        460        470        480 
NSSFLEDVYT FNDFIHDGIN KPLRKQQEVT GLEHNVPYLV TEYNGHMYPT KRFDNEERQM 

       490        500        510        520        530        540 
EHCLRHLRIQ NASYLDDSIS GAIGWCAFDY NTHKDFGSGD RICYHGVMDM FRLPKFASYV 

       550        560        570        580        590        600 
YKSQVSPDIE PILEPVTFWA RGERSIGGVI PLIIFTNCDY IELQYGNKTK IDNIYPNRDA 

       610        620        630        640        650        660 
YKGIPYPPII IDYDIVKPEM IGAWGMVWED LTLKGFYKGN KVIERKFSRE PIPTYLYVVP 

       670        680        690        700        710 
DDTILSATQK DATRIVVKIL DQYGNLLPFI NEVIKIEIEG PAKLQGPNEV ALIGGA 

« Hide

References

[1]"Cloning and analysis of the beta-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1."
Burchhardt G., Bahl H.
Gene 106:13-19(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
Strain: DSM 3896 / EM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57579 Genomic DNA. Translation: AAA23249.1.
PIRJU0275.

3D structure databases

ProteinModelPortalP26257.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-3780-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_THETU
AccessionPrimary (citable) accession number: P26257
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries