Reviewed, UniProtKB/Swiss-Prot P26236 (BCHM_RHOCA)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin O-methyltransferase
    EC=2.1.1.11
Alternative name(s):
    Magnesium-protoporphyrin IX methyltransferase

Gene names

Name:

bchM

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester using S-adenosyl-L-methionine as a cofactor.
Catalytic activity	S-adenosyl-L-methionine + magnesium protoporphyrin IX = S-adenosyl-L-homocysteine + magnesium protoporphyrin IX 13-methyl ester.
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the methyltransferase superfamily.
Caution	Was originally (Ref.1) thought to be involved in formation of the cyclopentone ring of protochlorophyllide from Mg-protoporphyrin IX monomethyl ester.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium protoporphyrin IX methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Magnesium-protoporphyrin O-methyltransferase		PRO_0000204420

Sequences

	Sequence		Length	Mass (Da)
	P26236-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: B32BB9B3DD3CF9EC	FASTA	224	25,225
	10 20 30 40 50 60 MPSDYAEIRN RVEHYFDRTA TRAWARLTTA DEKVSKVRQT VREGRDTMRA VMLSRLPDDL 70 80 90 100 110 120 TGCRVMDAGC GTGLTTVELA RRGADVVAVD ISPQLIDIAK DRLPPELRGK VSFHVGDMAD 130 140 150 160 170 180 PALGQFDYVV AMDSLIYYRA PDIGRVLTEL GKRTHSAIVF TVAPKTAFLM AFWWLGKLFP 190 200 210 220 RSNRSPVMIP HALDKLQRHA GDSLIKIDRV ARGFYISECL EYRP

References

[1]	"Nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from R. capsulata." Youvan D.C., Bylina E.J., Alberti M., Begusch H., Hearst J.E. Cell 37:949-957(1984) [PubMed: 6744416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Rhodobacter capsulatus genes involved in early steps of the bacteriochlorophyll biosynthetic pathway." Yang Z.M., Bauer C.E. J. Bacteriol. 172:5001-5010(1990) [PubMed: 2203738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
[3]	"Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase." Bollivar D.W., Jiang Z.Y., Bauer C.E., Beale S.I. J. Bacteriol. 176:5290-5296(1994) [PubMed: 8071204] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11165 Genomic DNA. Translation: CAA77522.1. K01183 Genomic DNA. No translation available. M34843 Genomic DNA. No translation available.
PIR	A28988.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.11. 567.
Family and domain databases
InterPro	IPR010251. BchM_ChlM. IPR010940. Mg_prot_MeTrfase_C. [Graphical view]
Pfam	PF07109. Mg-por_mtran_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR02021. BchM-ChlM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCHM_RHOCA

Accession

Primary (citable) accession number: P26236

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)