P26224 (GUNF_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase F
Short name=EGF
EC=3.2.1.4

Alternative name(s):
Cellulase F
Endo-1,4-beta-glucanase

Gene names

Name:	celF
Ordered Locus Names:	Cthe_0543

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Cofactor	Calcium.
Domain	A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 739	712	Endoglucanase F		PRO_0000007950
Regions
	Domain	480 – 639	160	CBM3
	Repeat	670 – 693	24	1
	Repeat	709 – 732	24	2
	Region	28 – 470	443	Catalytic
	Region	670 – 732	63	2 X 24 AA approximate repeats
Sites
	Active site	400	1	By similarity
	Active site	438	1	By similarity
	Active site	447	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26224 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0CD69EEFC6D4AEEF	FASTA	739	82,089
	10 20 30 40 50 60 MKKILAFLLT VALVAVVAIP QAVVSFAADF NYGEALQKAI MFYEFQRSGK LPENKRNNWR 70 80 90 100 110 120 GDSALNDGAD NGLDLTGGWY DAGDHVKFNL PMAYAVTMLA WSVYESRDAY VQSGQLPYIL 130 140 150 160 170 180 DNIKWATDYF IKCHPSPNVY YYQVGDGALD HSWWGPAEVM QMPRPSFKVD LTNPGSTVVA 190 200 210 220 230 240 ETAAAMAASS IVFKPTDPEY AATLLRHAKE LFTFADTTRS DAGYRAAEGY YSSHSGFYDE 250 260 270 280 290 300 LTWASIWLYL ATGDQSYLDK AESYEPHWER ERGTTLISYS WAHCWDNKLY GSLLLLAKIT 310 320 330 340 350 360 GKSYYKQCIE NHLDYWTVGF NGSRVQYTPK GLAYLDRWGS LRYATTQAFL ASVYADWSGC 370 380 390 400 410 420 DPAKAAVYKE FAKKQVDYAL GSTGRSFVVG FGKNPPRNPH HRTAHSSWSA LMTEPAECRH 430 440 450 460 470 480 ILVGALVGGP DGSDSYVDRL DDYQCNEVAN DYNAGFVGAL AKMYEKYGGE PIPNFVAFET 490 500 510 520 530 540 PGEEFYVEAA VNAAGPGFVN IKASIINKSG WPARGSDKLS AKYFVDISEA VAKGITLDQI 550 560 570 580 590 600 TVQSTTNGGA KVSQLLPWDP DNHIYYVNID FTGINIFPGG INEYKRDVYF TITAPYGEGN 610 620 630 640 650 660 WDNTNDFSFQ GLEQGFTSKK TEYIPLYDGN VRVWGKVPDG GSEPDPTPTI TVGPTPSVTP 670 680 690 700 710 720 TSVPGIMLGD VNFDGRINST DYSRLKRYVI KSLEFTDPEE HQKFIAAADV DGNGRINSTD 730 LYVLNRYILK LIEKFPAEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the cellulase gene celF of Clostridium thermocellum." Navarro A., Chebrou M.-C., Beguin P., Aubert J.-P. Res. Microbiol. 142:927-936(1991) [PubMed: 1805307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.
[3]	"Transcription of Clostridium thermocellum endoglucanase genes celF and celD." Mishra S., Beguin P., Aubert J.-P. J. Bacteriol. 173:80-85(1991) [PubMed: 1987137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60545 Genomic DNA. Translation: CAA43035.1. CP000568 Genomic DNA. Translation: ABN51779.1.
PIR	I40804. S15727.
RefSeq	YP_001036972.1. NC_009012.1.
3D structure databases
ProteinModelPortal	P26224.
SMR	P26224. Positions 30-638.
ModBase	Search...
Protein-protein interaction databases
STRING	P26224.
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4808292.
GenomeReviews	Gene locus Cthe_0543 in contig CP000568_GR.
KEGG	cth:Cthe_0543.
NMPDR	fig|203119.1.peg.1005.
PATRIC	19514745. VBICloThe47081_0565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG05134.
HOGENOM	HBG746347.
OMA	NEVANDY.
ProtClustDB	CLSK702792.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16419.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR008965. Carb-bd_dom. IPR001956. CBD_3. IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR018242. Dockerin_1. IPR018247. EF_Hand_1_Ca_BS. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:2.60.40.710. CBD_3. 1 hit. G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit.
Pfam	PF00942. CBM_3. 1 hit. PF00404. Dockerin_1. 2 hits. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SMART	SM01067. CBM_3. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF63446. Cellulos_enz_dockerin_1. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS51172. CBM3. 1 hit. PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 1 hit. Uncertain. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNF_CLOTH

Accession

Primary (citable) accession number: P26224
Secondary accession number(s): A3DCV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program