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Protein

Endoglucanase F

Gene

celF

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei400By similarity1
Active sitei438By similarity1
Active sitei447By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation
LigandCalcium

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-568-MONOMER
MetaCyc:MONOMER-16419

Protein family/group databases

CAZyiCBM3 Carbohydrate-Binding Module Family 3
GH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase F (EC:3.2.1.4)
Short name:
EGF
Alternative name(s):
Cellulase F
Endo-1,4-beta-glucanase
Gene namesi
Name:celF
Ordered Locus Names:Cthe_0543
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000000795028 – 739Endoglucanase FAdd BLAST712

Proteomic databases

PRIDEiP26224

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0543

Structurei

3D structure databases

ProteinModelPortaliP26224
SMRiP26224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini480 – 639CBM3PROSITE-ProRule annotationAdd BLAST160
Domaini664 – 737DockerinPROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 470CatalyticAdd BLAST443

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CYP Bacteria
ENOG410XPC9 LUCA
HOGENOMiHOG000021032
KOiK01179
OMAiDRSNYEQ
OrthoDBiPOG091H0908

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.60.40.710, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR018247 EF_Hand_1_Ca_BS
IPR001701 Glyco_hydro_9
IPR033126 Glyco_hydro_9_Asp/Glu_AS
IPR018221 Glyco_hydro_9_His_AS
PfamiView protein in Pfam
PF00942 CBM_3, 1 hit
PF00404 Dockerin_1, 2 hits
PF00759 Glyco_hydro_9, 1 hit
SMARTiView protein in SMART
SM01067 CBM_3, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF49384 SSF49384, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS51172 CBM3, 1 hit
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 1 hit
PS00592 GLYCOSYL_HYDROL_F9_1, 1 hit
PS00698 GLYCOSYL_HYDROL_F9_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKILAFLLT VALVAVVAIP QAVVSFAADF NYGEALQKAI MFYEFQRSGK
60 70 80 90 100
LPENKRNNWR GDSALNDGAD NGLDLTGGWY DAGDHVKFNL PMAYAVTMLA
110 120 130 140 150
WSVYESRDAY VQSGQLPYIL DNIKWATDYF IKCHPSPNVY YYQVGDGALD
160 170 180 190 200
HSWWGPAEVM QMPRPSFKVD LTNPGSTVVA ETAAAMAASS IVFKPTDPEY
210 220 230 240 250
AATLLRHAKE LFTFADTTRS DAGYRAAEGY YSSHSGFYDE LTWASIWLYL
260 270 280 290 300
ATGDQSYLDK AESYEPHWER ERGTTLISYS WAHCWDNKLY GSLLLLAKIT
310 320 330 340 350
GKSYYKQCIE NHLDYWTVGF NGSRVQYTPK GLAYLDRWGS LRYATTQAFL
360 370 380 390 400
ASVYADWSGC DPAKAAVYKE FAKKQVDYAL GSTGRSFVVG FGKNPPRNPH
410 420 430 440 450
HRTAHSSWSA LMTEPAECRH ILVGALVGGP DGSDSYVDRL DDYQCNEVAN
460 470 480 490 500
DYNAGFVGAL AKMYEKYGGE PIPNFVAFET PGEEFYVEAA VNAAGPGFVN
510 520 530 540 550
IKASIINKSG WPARGSDKLS AKYFVDISEA VAKGITLDQI TVQSTTNGGA
560 570 580 590 600
KVSQLLPWDP DNHIYYVNID FTGINIFPGG INEYKRDVYF TITAPYGEGN
610 620 630 640 650
WDNTNDFSFQ GLEQGFTSKK TEYIPLYDGN VRVWGKVPDG GSEPDPTPTI
660 670 680 690 700
TVGPTPSVTP TSVPGIMLGD VNFDGRINST DYSRLKRYVI KSLEFTDPEE
710 720 730
HQKFIAAADV DGNGRINSTD LYVLNRYILK LIEKFPAEQ
Length:739
Mass (Da):82,089
Last modified:May 1, 1992 - v1
Checksum:i0CD69EEFC6D4AEEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60545 Genomic DNA Translation: CAA43035.1
CP000568 Genomic DNA Translation: ABN51779.1
PIRiI40804
S15727
RefSeqiWP_003517595.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51779; ABN51779; Cthe_0543
GeneIDi35804292
KEGGicth:Cthe_0543

Similar proteinsi

Entry informationi

Entry nameiGUNF_CLOTH
AccessioniPrimary (citable) accession number: P26224
Secondary accession number(s): A3DCV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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