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P26221

- GUN4_THEFU

UniProt

P26221 - GUN4_THEFU

Protein

Endoglucanase E-4

Gene

celD

Organism
Thermobifida fusca (Thermomonospora fusca)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei427 – 4271By similarity
    Active sitei461 – 4611By similarity
    Active sitei470 – 4701By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    UniPathwayiUPA00696.

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    CBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase E-4 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase E-4
    Cellulase E4
    Endo-1,4-beta-glucanase E-4
    Gene namesi
    Name:celD
    OrganismiThermobifida fusca (Thermomonospora fusca)
    Taxonomic identifieri2021 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 46461 PublicationAdd
    BLAST
    Chaini47 – 880834Endoglucanase E-4PRO_0000007959Add
    BLAST

    Structurei

    Secondary structure

    1
    880
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi52 – 6514
    Turni85 – 884
    Helixi89 – 913
    Beta strandi102 – 1043
    Helixi109 – 12517
    Helixi127 – 1326
    Helixi136 – 15217
    Beta strandi159 – 1657
    Helixi167 – 1726
    Helixi177 – 1793
    Beta strandi186 – 1905
    Helixi196 – 21318
    Turni214 – 2163
    Helixi218 – 23720
    Helixi242 – 2443
    Helixi249 – 2524
    Helixi259 – 27315
    Helixi276 – 28510
    Helixi286 – 2883
    Beta strandi295 – 2984
    Beta strandi305 – 3073
    Helixi310 – 32112
    Helixi324 – 33613
    Turni337 – 3393
    Beta strandi358 – 3603
    Helixi361 – 37818
    Helixi382 – 40019
    Beta strandi413 – 4164
    Helixi424 – 4274
    Beta strandi430 – 4323
    Beta strandi436 – 4394
    Turni466 – 4694
    Helixi473 – 4764
    Helixi477 – 49014
    Beta strandi508 – 51710
    Beta strandi520 – 53112
    Beta strandi542 – 5509
    Helixi557 – 5593
    Beta strandi561 – 5633
    Beta strandi565 – 5695
    Beta strandi576 – 5794
    Beta strandi582 – 5887
    Beta strandi596 – 5983
    Turni599 – 6024
    Beta strandi603 – 6119
    Helixi618 – 6203
    Helixi622 – 6243
    Beta strandi637 – 6415
    Beta strandi644 – 6485

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JS4X-ray2.00A/B47-651[»]
    1TF4X-ray1.90A/B47-651[»]
    3TF4X-ray2.20A/B47-651[»]
    4TF4X-ray2.00A/B47-651[»]
    ProteinModelPortaliP26221.
    SMRiP26221. Positions 47-651.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26221.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini504 – 652149CBM3PROSITE-ProRule annotationAdd
    BLAST
    Domaini678 – 77093Fibronectin type-IIIPROSITE-ProRule annotationAdd
    BLAST
    Domaini771 – 880110CBM2Add
    BLAST

    Sequence similaritiesi

    Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation
    Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KOiK01179.
    OrthoDBiEOG6KQ6BP.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.60.40.10. 1 hit.
    2.60.40.290. 1 hit.
    2.60.40.710. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR003961. Fibronectin_type3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00942. CBM_3. 1 hit.
    PF00041. fn3. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SMARTiSM00637. CBD_II. 1 hit.
    SM01067. CBM_3. 1 hit.
    SM00060. FN3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49265. SSF49265. 1 hit.
    SSF49384. SSF49384. 2 hits.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS51172. CBM3. 1 hit.
    PS50853. FN3. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVTEPPPRR RGRHSRARRF LTSLGATAAL TAGMLGVPLA TGTAHAEPAF    50
    NYAEALQKSM FFYEAQRSGK LPENNRVSWR GDSGLNDGAD VGLDLTGGWY 100
    DAGDHVKFGF PMAFTATMLA WGAIESPEGY IRSGQMPYLK DNLRWVNDYF 150
    IKAHPSPNVL YVQVGDGDAD HKWWGPAEVM PMERPSFKVD PSCPGSDVAA 200
    ETAAAMAASS IVFADDDPAY AATLVQHAKQ LYTFADTYRG VYSDCVPAGA 250
    FYNSWSGYQD ELVWGAYWLY KATGDDSYLA KAEYEYDFLS TEQQTDLRSY 300
    RWTIAWDDKS YGTYVLLAKE TGKQKYIDDA NRWLDYWTVG VNGQRVPYSP 350
    GGMAVLDTWG ALRYAANTAF VALVYAKVID DPVRKQRYHD FAVRQINYAL 400
    GDNPRNSSYV VGFGNNPPRN PHHRTAHGSW TDSIASPAEN RHVLYGALVG 450
    GPGSPNDAYT DDRQDYVANE VATDYNAGFS SALAMLVEEY GGTPLADFPP 500
    TEEPDGPEIF VEAQINTPGT TFTEIKAMIR NQSGWPARML DKGTFRYWFT 550
    LDEGVDPADI TVSSAYNQCA TPEDVHHVSG DLYYVEIDCT GEKIFPGGQS 600
    EHRREVQFRI AGGPGWDPSN DWSFQGIGNE LAPAPYIVLY DDGVPVWGTA 650
    PEEGEEPGGG EGPGGGEEPG EDVTPPSAPG SPAVRDVTST SAVLTWSASS 700
    DTGGSGVAGY DVFLRAGTGQ EQKVGSTTRT SFTLTGLEPD TTYIAAVVAR 750
    DNAGNVSQRS TVSFTTLAEN GGGPDASCTV GYSTNDWDSG FTASIRITYH 800
    GTAPLSSWEL SFTFPAGQQV THGWNATWRQ DGAAVTATPM SWNSSLAPGA 850
    TVEVGFNGSW SGSNTPPTDF TLNGEPCALA 880
    Length:880
    Mass (Da):95,203
    Last modified:November 1, 1997 - v2
    Checksum:i5EA9A6ABF45A4D9A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20093 Genomic DNA. Translation: AAB42155.1.
    PIRiB42360.
    RefSeqiYP_290232.1. NC_007333.1.

    Genome annotation databases

    GeneIDi3580397.
    KEGGitfu:Tfu_2176.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20093 Genomic DNA. Translation: AAB42155.1 .
    PIRi B42360.
    RefSeqi YP_290232.1. NC_007333.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JS4 X-ray 2.00 A/B 47-651 [» ]
    1TF4 X-ray 1.90 A/B 47-651 [» ]
    3TF4 X-ray 2.20 A/B 47-651 [» ]
    4TF4 X-ray 2.00 A/B 47-651 [» ]
    ProteinModelPortali P26221.
    SMRi P26221. Positions 47-651.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM2. Carbohydrate-Binding Module Family 2.
    CBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3580397.
    KEGGi tfu:Tfu_2176.

    Phylogenomic databases

    KOi K01179.
    OrthoDBi EOG6KQ6BP.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .

    Miscellaneous databases

    EvolutionaryTracei P26221.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    2.60.40.10. 1 hit.
    2.60.40.290. 1 hit.
    2.60.40.710. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR003961. Fibronectin_type3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00553. CBM_2. 1 hit.
    PF00942. CBM_3. 1 hit.
    PF00041. fn3. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SMARTi SM00637. CBD_II. 1 hit.
    SM01067. CBM_3. 1 hit.
    SM00060. FN3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49265. SSF49265. 1 hit.
    SSF49384. SSF49384. 2 hits.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS51172. CBM3. 1 hit.
    PS50853. FN3. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca."
      Jung E.D., Lao G., Irwin D., Barr B.K., Benjamin A., Wilson D.B.
      Appl. Environ. Microbiol. 59:3032-3043(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YX.
    2. Wilson D.B.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca."
      Lao G., Ghangas G.S., Jung E.D., Wilson D.B.
      J. Bacteriol. 173:3397-3407(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YX.
    4. "Cellulases of Thermomonospora fusca."
      Wilson D.B.
      Methods Enzymol. 160:314-323(1988)
      Cited for: PROTEIN SEQUENCE OF 47-67.
    5. "Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca."
      Sakon J., Irwin D., Wilson D.B., Karplus P.A.
      Nat. Struct. Biol. 4:810-818(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 47-651.

    Entry informationi

    Entry nameiGUN4_THEFU
    AccessioniPrimary (citable) accession number: P26221
    Secondary accession number(s): Q08167
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3