Endoglucanase E-4 precursor - Thermomonospora fusca

Names and origin

Protein names

Recommended name:
    Endoglucanase E-4
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase E-4
    Cellulase E-4
    Cellulase E4

Gene names

Name:

celD

Organism

Thermomonospora fusca

Taxonomic identifier

2021 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptosporangineae › Nocardiopsaceae › Thermobifida

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Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathway

Glycan metabolism; cellulose degradation.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Contains 1 CBM3 (carbohydrate binding type-3) domain.

Contains 1 fibronectin type-III domain.

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Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 46

46

Ref.4

Chain

47 – 880

834

Endoglucanase E-4

PRO_0000007959

Regions

Domain

504 – 652

149

CBM3

Domain

675 – 766

92

Fibronectin type-III

Domain

771 – 880

110

CBM2

Sites

Active site

427

1

By similarity

Active site

461

1

By similarity

Active site

470

1

By similarity

Secondary structure

1

.

880

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P26221-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 5EA9A6ABF45A4D9A
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FASTA

880

95,203

        10         20         30         40         50         60 
MSVTEPPPRR RGRHSRARRF LTSLGATAAL TAGMLGVPLA TGTAHAEPAF NYAEALQKSM 

        70         80         90        100        110        120 
FFYEAQRSGK LPENNRVSWR GDSGLNDGAD VGLDLTGGWY DAGDHVKFGF PMAFTATMLA 

       130        140        150        160        170        180 
WGAIESPEGY IRSGQMPYLK DNLRWVNDYF IKAHPSPNVL YVQVGDGDAD HKWWGPAEVM 

       190        200        210        220        230        240 
PMERPSFKVD PSCPGSDVAA ETAAAMAASS IVFADDDPAY AATLVQHAKQ LYTFADTYRG 

       250        260        270        280        290        300 
VYSDCVPAGA FYNSWSGYQD ELVWGAYWLY KATGDDSYLA KAEYEYDFLS TEQQTDLRSY 

       310        320        330        340        350        360 
RWTIAWDDKS YGTYVLLAKE TGKQKYIDDA NRWLDYWTVG VNGQRVPYSP GGMAVLDTWG 

       370        380        390        400        410        420 
ALRYAANTAF VALVYAKVID DPVRKQRYHD FAVRQINYAL GDNPRNSSYV VGFGNNPPRN 

       430        440        450        460        470        480 
PHHRTAHGSW TDSIASPAEN RHVLYGALVG GPGSPNDAYT DDRQDYVANE VATDYNAGFS 

       490        500        510        520        530        540 
SALAMLVEEY GGTPLADFPP TEEPDGPEIF VEAQINTPGT TFTEIKAMIR NQSGWPARML 

       550        560        570        580        590        600 
DKGTFRYWFT LDEGVDPADI TVSSAYNQCA TPEDVHHVSG DLYYVEIDCT GEKIFPGGQS 

       610        620        630        640        650        660 
EHRREVQFRI AGGPGWDPSN DWSFQGIGNE LAPAPYIVLY DDGVPVWGTA PEEGEEPGGG 

       670        680        690        700        710        720 
EGPGGGEEPG EDVTPPSAPG SPAVRDVTST SAVLTWSASS DTGGSGVAGY DVFLRAGTGQ 

       730        740        750        760        770        780 
EQKVGSTTRT SFTLTGLEPD TTYIAAVVAR DNAGNVSQRS TVSFTTLAEN GGGPDASCTV 

       790        800        810        820        830        840 
GYSTNDWDSG FTASIRITYH GTAPLSSWEL SFTFPAGQQV THGWNATWRQ DGAAVTATPM 

       850        860        870        880 
SWNSSLAPGA TVEVGFNGSW SGSNTPPTDF TLNGEPCALA

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References

[1]	"DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca." Jung E.D., Lao G., Irwin D., Barr B.K., Benjamin A., Wilson D.B. Appl. Environ. Microbiol. 59:3032-3043(1993) [PubMed: 8215374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YX.
[2]	Wilson D.B. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca." Lao G., Ghangas G.S., Jung E.D., Wilson D.B. J. Bacteriol. 173:3397-3407(1991) [PubMed: 1904434] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YX.
[4]	"Cellulases of Thermomonospora fusca." Wilson D.B. Methods Enzymol. 160:314-323(1988) Cited for: PROTEIN SEQUENCE OF 47-67.
[5]	"Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca." Sakon J., Irwin D., Wilson D.B., Karplus P.A. Nat. Struct. Biol. 4:810-818(1997) [PubMed: 9334746] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 47-651.

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Cross-references

Sequence databases

L20093 Genomic DNA. Translation: AAB42155.1.

PIR

B42360.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JS4	X-ray	2.00	A/B	47-651	[»]
1TF4	X-ray	1.90	A/B	47-651	[»]
3TF4	X-ray	2.20	A/B	47-651	[»]
4TF4	X-ray	2.00	A/B	47-651	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM2. Carbohydrate-Binding Module Family 2.
CBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Family and domain databases

InterPro

IPR012341. 6hp_glycosidase.
IPR001956. CBD_3.
IPR001919. CBD_bac.
IPR018366. CBM2_CS.
IPR003961. FN_III.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]

Gene3D

G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.

PANTHER

PTHR22298:SF3. Glyco_hydro_9. 1 hit.

Pfam

PF00553. CBM_2. 1 hit.
PF00942. CBM_3. 1 hit.
PF00041. fn3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]

ProDom

PD001947. CBD_3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00637. CBD_II. 1 hit.
SM00060. FN3. 1 hit.
[Graphical view]

PROSITE

PS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS51172. CBM3. 1 hit.
PS50853. FN3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

GUN4_THEFU

Accession

Primary (citable) accession number: P26221
Secondary accession number(s): Q08167

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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