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P26221

- GUN4_THEFU

UniProt

P26221 - GUN4_THEFU

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Protein

Endoglucanase E-4

Gene

celD

Organism
Thermobifida fusca (Thermomonospora fusca)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei427 – 4271By similarity
Active sitei461 – 4611By similarity
Active sitei470 – 4701By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00696.

Protein family/group databases

CAZyiCBM2. Carbohydrate-Binding Module Family 2.
CBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase E-4 (EC:3.2.1.4)
Alternative name(s):
Cellulase E-4
Cellulase E4
Endo-1,4-beta-glucanase E-4
Gene namesi
Name:celD
OrganismiThermobifida fusca (Thermomonospora fusca)
Taxonomic identifieri2021 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeThermobifida

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 46461 PublicationAdd
BLAST
Chaini47 – 880834Endoglucanase E-4PRO_0000007959Add
BLAST

Structurei

Secondary structure

1
880
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi52 – 6514
Turni85 – 884
Helixi89 – 913
Beta strandi102 – 1043
Helixi109 – 12517
Helixi127 – 1326
Helixi136 – 15217
Beta strandi159 – 1657
Helixi167 – 1726
Helixi177 – 1793
Beta strandi186 – 1905
Helixi196 – 21318
Turni214 – 2163
Helixi218 – 23720
Helixi242 – 2443
Helixi249 – 2524
Helixi259 – 27315
Helixi276 – 28510
Helixi286 – 2883
Beta strandi295 – 2984
Beta strandi305 – 3073
Helixi310 – 32112
Helixi324 – 33613
Turni337 – 3393
Beta strandi358 – 3603
Helixi361 – 37818
Helixi382 – 40019
Beta strandi413 – 4164
Helixi424 – 4274
Beta strandi430 – 4323
Beta strandi436 – 4394
Turni466 – 4694
Helixi473 – 4764
Helixi477 – 49014
Beta strandi508 – 51710
Beta strandi520 – 53112
Beta strandi542 – 5509
Helixi557 – 5593
Beta strandi561 – 5633
Beta strandi565 – 5695
Beta strandi576 – 5794
Beta strandi582 – 5887
Beta strandi596 – 5983
Turni599 – 6024
Beta strandi603 – 6119
Helixi618 – 6203
Helixi622 – 6243
Beta strandi637 – 6415
Beta strandi644 – 6485

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JS4X-ray2.00A/B47-651[»]
1TF4X-ray1.90A/B47-651[»]
3TF4X-ray2.20A/B47-651[»]
4TF4X-ray2.00A/B47-651[»]
ProteinModelPortaliP26221.
SMRiP26221. Positions 47-651.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26221.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini504 – 652149CBM3PROSITE-ProRule annotationAdd
BLAST
Domaini678 – 77093Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST
Domaini771 – 880110CBM2Add
BLAST

Sequence similaritiesi

Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01179.
OrthoDBiEOG6KQ6BP.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
2.60.40.290. 1 hit.
2.60.40.710. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR003961. Fibronectin_type3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00553. CBM_2. 1 hit.
PF00942. CBM_3. 1 hit.
PF00041. fn3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM00637. CBD_II. 1 hit.
SM01067. CBM_3. 1 hit.
SM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 2 hits.
PROSITEiPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS51172. CBM3. 1 hit.
PS50853. FN3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26221-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVTEPPPRR RGRHSRARRF LTSLGATAAL TAGMLGVPLA TGTAHAEPAF
60 70 80 90 100
NYAEALQKSM FFYEAQRSGK LPENNRVSWR GDSGLNDGAD VGLDLTGGWY
110 120 130 140 150
DAGDHVKFGF PMAFTATMLA WGAIESPEGY IRSGQMPYLK DNLRWVNDYF
160 170 180 190 200
IKAHPSPNVL YVQVGDGDAD HKWWGPAEVM PMERPSFKVD PSCPGSDVAA
210 220 230 240 250
ETAAAMAASS IVFADDDPAY AATLVQHAKQ LYTFADTYRG VYSDCVPAGA
260 270 280 290 300
FYNSWSGYQD ELVWGAYWLY KATGDDSYLA KAEYEYDFLS TEQQTDLRSY
310 320 330 340 350
RWTIAWDDKS YGTYVLLAKE TGKQKYIDDA NRWLDYWTVG VNGQRVPYSP
360 370 380 390 400
GGMAVLDTWG ALRYAANTAF VALVYAKVID DPVRKQRYHD FAVRQINYAL
410 420 430 440 450
GDNPRNSSYV VGFGNNPPRN PHHRTAHGSW TDSIASPAEN RHVLYGALVG
460 470 480 490 500
GPGSPNDAYT DDRQDYVANE VATDYNAGFS SALAMLVEEY GGTPLADFPP
510 520 530 540 550
TEEPDGPEIF VEAQINTPGT TFTEIKAMIR NQSGWPARML DKGTFRYWFT
560 570 580 590 600
LDEGVDPADI TVSSAYNQCA TPEDVHHVSG DLYYVEIDCT GEKIFPGGQS
610 620 630 640 650
EHRREVQFRI AGGPGWDPSN DWSFQGIGNE LAPAPYIVLY DDGVPVWGTA
660 670 680 690 700
PEEGEEPGGG EGPGGGEEPG EDVTPPSAPG SPAVRDVTST SAVLTWSASS
710 720 730 740 750
DTGGSGVAGY DVFLRAGTGQ EQKVGSTTRT SFTLTGLEPD TTYIAAVVAR
760 770 780 790 800
DNAGNVSQRS TVSFTTLAEN GGGPDASCTV GYSTNDWDSG FTASIRITYH
810 820 830 840 850
GTAPLSSWEL SFTFPAGQQV THGWNATWRQ DGAAVTATPM SWNSSLAPGA
860 870 880
TVEVGFNGSW SGSNTPPTDF TLNGEPCALA
Length:880
Mass (Da):95,203
Last modified:November 1, 1997 - v2
Checksum:i5EA9A6ABF45A4D9A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L20093 Genomic DNA. Translation: AAB42155.1.
PIRiB42360.
RefSeqiYP_290232.1. NC_007333.1.

Genome annotation databases

GeneIDi3580397.
KEGGitfu:Tfu_2176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L20093 Genomic DNA. Translation: AAB42155.1 .
PIRi B42360.
RefSeqi YP_290232.1. NC_007333.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JS4 X-ray 2.00 A/B 47-651 [» ]
1TF4 X-ray 1.90 A/B 47-651 [» ]
3TF4 X-ray 2.20 A/B 47-651 [» ]
4TF4 X-ray 2.00 A/B 47-651 [» ]
ProteinModelPortali P26221.
SMRi P26221. Positions 47-651.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM2. Carbohydrate-Binding Module Family 2.
CBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3580397.
KEGGi tfu:Tfu_2176.

Phylogenomic databases

KOi K01179.
OrthoDBi EOG6KQ6BP.

Enzyme and pathway databases

UniPathwayi UPA00696 .

Miscellaneous databases

EvolutionaryTracei P26221.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
2.60.40.290. 1 hit.
2.60.40.710. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR003961. Fibronectin_type3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00553. CBM_2. 1 hit.
PF00942. CBM_3. 1 hit.
PF00041. fn3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SMARTi SM00637. CBD_II. 1 hit.
SM01067. CBM_3. 1 hit.
SM00060. FN3. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 2 hits.
PROSITEi PS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS51172. CBM3. 1 hit.
PS50853. FN3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca."
    Jung E.D., Lao G., Irwin D., Barr B.K., Benjamin A., Wilson D.B.
    Appl. Environ. Microbiol. 59:3032-3043(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YX.
  2. Wilson D.B.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca."
    Lao G., Ghangas G.S., Jung E.D., Wilson D.B.
    J. Bacteriol. 173:3397-3407(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YX.
  4. "Cellulases of Thermomonospora fusca."
    Wilson D.B.
    Methods Enzymol. 160:314-323(1988)
    Cited for: PROTEIN SEQUENCE OF 47-67.
  5. "Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca."
    Sakon J., Irwin D., Wilson D.B., Karplus P.A.
    Nat. Struct. Biol. 4:810-818(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 47-651.

Entry informationi

Entry nameiGUN4_THEFU
AccessioniPrimary (citable) accession number: P26221
Secondary accession number(s): Q08167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: October 1, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3