Reviewed, UniProtKB/Swiss-Prot P26216 (PGLR1_MAIZE)

Names and origin

Protein names

Recommended name:
    Exopolygalacturonase
      Short name=ExoPG
    EC=3.2.1.67
Alternative name(s):
    Pectinase
    Galacturan 1,4-alpha-galacturonidase

Gene names

Name:	PG1
AND
Name:	PG2
AND
Name:	PG3
AND
Name:	PG6
AND
Name:	PG14

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.
Catalytic activity	((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.
Subcellular location	Secreted. Secreted › cell wall.
Tissue specificity	Pollen.
Developmental stage	Late stages of pollen development.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galacturan 1,4-alpha-galacturonidase activity Inferred from electronic annotation. Source: EC polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 410	388	Exopolygalacturonase		PRO_0000024806
Sites
	Active site	233	1	Proton donor By similarity
	Active site	256	1	By similarity
Amino acid modifications
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
	Glycosylation	349	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	359	1	A → V in PG14.

Sequences

	Sequence		Length	Mass (Da)
	P26216-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 0A6E779644D818BA	FASTA	410	43,444
	10 20 30 40 50 60 MACTNNAMRA LFLLVLFCIV HGEKEESKGI DAKASGPGGS FDITKLGASG NGKTDSTKAV 70 80 90 100 110 120 QEAWASACGG TGKQTILIPK GDFLVGQLNF TGPCKGDVTI QVDGNLLATT DLSQYKDHGN 130 140 150 160 170 180 WIEILRVDNL VITGKGNLDG QGPAVWSKNS CTKKYDCKIL PNSLVMDFVN NGEVSGVTLL 190 200 210 220 230 240 NSKFFHMNMY RCKDMLIKDV TVTAPGDSPN TDGIHMGDSS GITITNTVIG VGDDCISIGP 250 260 270 280 290 300 GTSKVNITGV TCGPGHGISI GSLGRYKDEK DVTDINVKDC TLKKTMFGVR IKAYEDAASV 310 320 330 340 350 360 LTVSKIHYEN IKMEDSANPI FIDMKYCPNK LCTANGASKV TVKDVTFKNI TGTSSTPEAV 370 380 390 400 410 SLLCTAKVPC TGVTMDDVNV EYSGTNNKTM AICTNAKGST KGCLKELACF

References

[1]	"Characterization of pollen polygalacturonase encoded by several cDNA clones in maize." Niogret M.F., Dubald M., Mandaron P., Mache R. Plant Mol. Biol. 17:1155-1164(1991) [PubMed: 1932692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-37. Strain: cv. Missouri 17. Tissue: Pollen.
[2]	"Characterization of a multigene family encoding an exopolygalacturonase in maize." Barakate A., Martin W., Quigley F., Mache R. J. Mol. Biol. 229:797-801(1993) [PubMed: 8433375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Missouri 17. Tissue: Leaf.
[3]	"Sequence analysis of three members of the maize polygalacturonase gene family expressed during pollen development." Allen R.L., Lonsdale D.M. Plant Mol. Biol. 20:343-345(1992) [PubMed: 1391780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. B73 and cv. Wisconsin 22.
[4]	"Molecular characterization of one of the maize polygalacturonase gene family members which are expressed during late pollen development." Allen R.L., Lonsdale D.M. Plant J. 3:261-271(1993) [PubMed: 8106080] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-306.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57627 mRNA. Translation: CAA40850.1. X57628 mRNA. Translation: CAA40851.1. Different initiation. X57743 mRNA. Translation: CAA40910.1. X57575 mRNA. Translation: CAA40803.1. X65844 Genomic DNA. Translation: CAA46679.1. X65845 Genomic DNA. Translation: CAA46680.1. X62384 Genomic DNA. Translation: CAA44248.1. X62385 Genomic DNA. Translation: CAA44249.1. X66692 Genomic DNA. Translation: CAA47234.1.
PIR	S18570. S25824. S30064.
RefSeq	NP_001105432.1.
UniGene	Zm.3554
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Genome annotation databases
GeneID	542387.
Organism-specific databases
Gramene	P26216.
MaizeGDB	25864.
Enzyme and pathway databases
BRENDA	3.2.1.67. 289.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR1_MAIZE

Accession

Primary (citable) accession number: P26216

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)