P26215 (PGLR_COCCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase

Gene names

Name:

PGN1

Organism

Cochliobolus carbonum (Bipolaris zeicola)

Taxonomic identifier

5017 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Pleosporaceae › Cochliobolus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 27	6	Potential		PRO_0000024782
	Chain	28 – 364	337	Polygalacturonase		PRO_0000024783
Regions
	Repeat	158 – 188	31	PbH1 1
	Repeat	189 – 210	22	PbH1 2
	Repeat	211 – 231	21	PbH1 3
	Repeat	240 – 261	22	PbH1 4
	Repeat	269 – 291	23	PbH1 5
	Repeat	303 – 348	46	PbH1 6
Sites
	Active site	203	1	Proton donor By similarity
	Active site	225	1	By similarity
Amino acid modifications
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 45		By similarity
	Disulfide bond	205 ↔ 221		By similarity
	Disulfide bond	331 ↔ 336		By similarity
	Disulfide bond	355 ↔ 364		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26215 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 501CC8E31F03A304	FASTA	364	36,597
	10 20 30 40 50 60 MVAYALTSML LSAGALVAAA PSGLDARDGC TFTDAATAIK NKASCSNIVI SGMTVPAGTT 70 80 90 100 110 120 LDLTGLKSGA TVTFQGTTTF GYKEWEGPLI SVSGTNIKVV GASGHTIDAA GQKWWDGKGS 130 140 150 160 170 180 NGGKTKPKFF YAHSLTTSSI SGLNIKNTPV QAFSINGVTG LTLDRITIDN SAGDSAGAHN 190 200 210 220 230 240 TDAFDIGSSS GITISNANIK NQDDCVAINS GSDIHVTNCQ CSGGHGVSIG SVGGRKDNTV 250 260 270 280 290 300 KGVVVSGTTI ANSDNGVRIK TISGATGSVS DITYENITLK NIAKYGIVIE QDYLNGGPTG 310 320 330 340 350 360 KPTTGVPITG VTLKNVAGSV TGSGTEIYVL CGKGSCSGWN WSGVSITGGK KSSSCLNVPS GASC

References

[1]	"Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize." Scott-Craig J.S., Panaccione D.G., Cervone F., Walton J.D. Plant Cell 2:1191-1200(1990) [PubMed: 2152162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55979 Genomic DNA. Translation: AAA79885.1.
PIR	S28771.
3D structure databases
ProteinModelPortal	P26215.
SMR	P26215. Positions 30-362.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_COCCA

Accession

Primary (citable) accession number: P26215

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 31, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program