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Protein

Endopolygalacturonase I

Gene

pgaI

Organism
Aspergillus niger
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei207Proton donorPROSITE-ProRule annotation1
Active sitei229Curated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi3.2.1.15. 518.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28I_ASPNG.

Names & Taxonomyi

Protein namesi
Recommended name:
Endopolygalacturonase I (EC:3.2.1.15)
Alternative name(s):
Pectinase 1
Polygalacturonase I
Short name:
PG-I
Gene namesi
Name:pgaI
Synonyms:pg1, pga1
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei8269. Asp n Pectinase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002476819 – 31Sequence analysisAdd BLAST13
ChainiPRO_000002476932 – 368Endopolygalacturonase IAdd BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 50
Glycosylationi44O-linked (Man)1 Publication1
Glycosylationi46O-linked (Man)1 Publication1
Disulfide bondi209 ↔ 225
Glycosylationi246N-linked (GlcNAc...)1 Publication1
Disulfide bondi335 ↔ 340
Disulfide bondi359 ↔ 368

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP26213.
PRIDEiP26213.

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00001113.

Structurei

Secondary structure

1368
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 39Combined sources5
Helixi40 – 46Combined sources7
Helixi47 – 49Combined sources3
Beta strandi51 – 57Combined sources7
Beta strandi76 – 85Combined sources10
Beta strandi94 – 96Combined sources3
Beta strandi99 – 101Combined sources3
Beta strandi103 – 106Combined sources4
Beta strandi111 – 113Combined sources3
Helixi116 – 118Combined sources3
Turni124 – 126Combined sources3
Beta strandi127 – 129Combined sources3
Beta strandi135 – 147Combined sources13
Beta strandi149 – 151Combined sources3
Beta strandi158 – 169Combined sources12
Beta strandi171 – 173Combined sources3
Helixi177 – 180Combined sources4
Beta strandi187 – 190Combined sources4
Beta strandi194 – 200Combined sources7
Beta strandi202 – 222Combined sources21
Beta strandi224 – 240Combined sources17
Beta strandi243 – 256Combined sources14
Beta strandi258 – 266Combined sources9
Beta strandi272 – 298Combined sources27
Beta strandi308 – 310Combined sources3
Beta strandi312 – 324Combined sources13
Beta strandi329 – 334Combined sources6
Beta strandi340 – 354Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHCX-ray1.70A/B/C/D/E/F33-368[»]
ProteinModelPortaliP26213.
SMRiP26213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati140 – 161PbH1 1Add BLAST22
Repeati162 – 192PbH1 2Add BLAST31
Repeati193 – 214PbH1 3Add BLAST22
Repeati244 – 265PbH1 4Add BLAST22
Repeati273 – 295PbH1 5Add BLAST23
Repeati307 – 352PbH1 6Add BLAST46

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG5434. LUCA.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSYQLLGLA AVGSLVSAAP APSRVSEFAK KASTCTFTSA SEASESISSC
60 70 80 90 100
SDVVLSSIEV PAGETLDLSD AADGSTITFE GTTSFGYKEW KGPLIRFGGK
110 120 130 140 150
DLTVTMADGA VIDGDGSRWW DSKGTNGGKT KPKFMYIHDV EDSTFKGINI
160 170 180 190 200
KNTPVQAISV QATNVHLNDF TIDNSDGDDN GGHNTDGFDI SESTGVYISG
210 220 230 240 250
ATVKNQDDCI AINSGESISF TGGTCSGGHG LSIGSVGGRD DNTVKNVTIS
260 270 280 290 300
DSTVSNSANG VRIKTIYKET GDVSEITYSN IQLSGITDYG IVIEQDYENG
310 320 330 340 350
SPTGTPSTGI PITDVTVDGV TGTLEDDATQ VYILCGDGSC SDWTWSGVDL
360
SGGKTSDKCE NVPSGASC
Length:368
Mass (Da):38,108
Last modified:May 1, 1992 - v1
Checksum:iCD9B46A9A99B5102
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58892 Genomic DNA. Translation: CAA41693.1.
PIRiS17980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58892 Genomic DNA. Translation: CAA41693.1.
PIRiS17980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHCX-ray1.70A/B/C/D/E/F33-368[»]
ProteinModelPortaliP26213.
SMRiP26213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5061.CADANGAP00001113.

Protein family/group databases

Allergomei8269. Asp n Pectinase.
CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28I_ASPNG.

Proteomic databases

PaxDbiP26213.
PRIDEiP26213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG5434. LUCA.

Enzyme and pathway databases

BRENDAi3.2.1.15. 518.

Miscellaneous databases

EvolutionaryTraceiP26213.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR1_ASPNG
AccessioniPrimary (citable) accession number: P26213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.