Reviewed, UniProtKB/Swiss-Prot P26208 (BGLA_CLOTH)

Names and origin

Protein names

Recommended name:
    Beta-glucosidase A
    EC=3.2.1.21
Alternative name(s):
    Gentiobiase
    Cellobiase
    Beta-D-glucoside glucohydrolase

Gene names

Name:	bglA
Ordered Locus Names:	Cthe_0212

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Beta-glucosidase A		PRO_0000063876
Sites
	Active site	166	1	Proton donor Potential
	Active site	355	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26208-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: D59B9EB7BEE67621	FASTA	448	51,482
	10 20 30 40 50 60 MSKITFPKDF IWGSATAAYQ IEGAYNEDGK GESIWDRFSH TPGNIADGHT GDVACDHYHR 70 80 90 100 110 120 YEEDIKIMKE IGIKSYRFSI SWPRIFPEGT GKLNQKGLDF YKRLTNLLLE NGIMPAITLY 130 140 150 160 170 180 HWDLPQKLQD KGGWKNRDTT DYFTEYSEVI FKNLGDIVPI WFTHNEPGVV SLLGHFLGIH 190 200 210 220 230 240 APGIKDLRTS LEVSHNLLLS HGKAVKLFRE MNIDAQIGIA LNLSYHYPAS EKAEDIEAAE 250 260 270 280 290 300 LSFSLAGRWY LDPVLKGRYP ENALKLYKKK GIELSFPEDD LKLISQPIDF IAFNNYSSEF 310 320 330 340 350 360 IKYDPSSESG FSPANSILEK FEKTDMGWII YPEGLYDLLM LLDRDYGKPN IVISENGAAF 370 380 390 400 410 420 KDEIGSNGKI EDTKRIQYLK DYLTQAHRAI QDGVNLKAYY LWSLLDNFEW AYGYNKRFGI 430 440 VHVNFDTLER KIKDSGYWYK EVIKNNGF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the beta-glucosidase gene bglA of Clostridium thermocellum. Sequence analysis reveals a superfamily of cellulases and beta-glycosidases including human lactase/phlorizin hydrolase." Graebnitz F., Seiss M., Ruecknagel K.P., Staudenbauer W.L. Eur. J. Biochem. 200:301-309(1991) [PubMed: 1909624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60268 Genomic DNA. Translation: CAA42814.1. CP000568 Genomic DNA. Translation: ABN51453.1. Different initiation.
PIR	S17215.
RefSeq	YP_001036646.1.
3D structure databases
HSSP	HSSP built from PDB template 1OD0 based on UniProtKB Q08638.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	4808630.
GenomeReviews	Gene locus Cthe_0212 in contig CP000568_GR.
KEGG	cth:Cthe_0212.
NMPDR	fig|203119.1.peg.2308.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	P26208. WAGDFRD.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017736. Glyco_hydro_1_beta-glucosidase. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR03356. BGL. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLA_CLOTH

Accession

Primary (citable) accession number: P26208
Secondary accession number(s): A3DBX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)