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Protein

Platelet glycoprotein 4

Gene

CD36

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei463 – 4631Critical for TLR4-TLR6 dimerization and signalingBy similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Cell adhesion, Transport

Enzyme and pathway databases

ReactomeiREACT_272065. Platelet degranulation.
REACT_303007. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_305366. MyD88:Mal cascade initiated on plasma membrane.
REACT_318860. Cross-presentation of particulate exogenous antigens (phagosomes).
REACT_353291. Scavenging by Class B Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet glycoprotein 4
Alternative name(s):
Glycoprotein IIIb
Short name:
GPIIIB
PAS IV
PAS-4
Platelet glycoprotein IV
Short name:
GPIV
CD_antigen: CD36
Gene namesi
Name:CD36
Synonyms:PAS4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 4

Subcellular locationi

  • Cell membrane; Multi-pass membrane protein

  • Note: Upon ligand-binding, internalized through dynamin-dependent endocytosis.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 76CytoplasmicSequence Analysis
Transmembranei8 – 2922HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 439410ExtracellularSequence AnalysisAdd
BLAST
Transmembranei440 – 46122HelicalSequence AnalysisAdd
BLAST
Topological domaini462 – 47211CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 472471Platelet glycoprotein 4PRO_0000144150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Lipidationi7 – 71S-palmitoyl cysteineBy similarity
Glycosylationi79 – 791N-linked (GlcNAc...)1 Publication
Glycosylationi102 – 1021N-linked (GlcNAc...)1 Publication
Glycosylationi172 – 1721N-linked (GlcNAc...)1 Publication
Glycosylationi205 – 2051N-linked (GlcNAc...)1 Publication
Glycosylationi235 – 2351N-linked (GlcNAc...)1 Publication
Disulfide bondi243 ↔ 3111 Publication
Glycosylationi247 – 2471N-linked (GlcNAc...)1 Publication
Disulfide bondi272 ↔ 3331 Publication
Disulfide bondi313 ↔ 3221 Publication
Glycosylationi321 – 3211N-linked (GlcNAc...)1 Publication
Glycosylationi417 – 4171N-linked (GlcNAc...)1 Publication
Lipidationi464 – 4641S-palmitoyl cysteineBy similarity
Lipidationi466 – 4661S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP26201.

PTM databases

UniCarbKBiP26201.

Interactioni

Subunit structurei

Interacts with THBS1 and THBS2; the interactions mediate the THBS antiangiogenic activity. Upon interaction with a ligand, such as oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42, rapidly forms a complex with TLR4 and TLR6; the complex is internalized and triggers an inflammatory signal. Through its C-terminus, interacts with PTK2, PXN and LYN, but not with SRC. LYN kinase activity is required for facilitating TLR4-TLR6 heterodimerization and signal initiation.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023750.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 12028Required for interaction with thrombospondins, THBS1 and THBS2Add
BLAST
Regioni460 – 47213Interaction with PTK2, PXN and LYNBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CD36 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257244.
GeneTreeiENSGT00530000062927.
HOGENOMiHOG000252951.
HOVERGENiHBG002754.
InParanoidiP26201.
KOiK06259.
OMAiKRNYIVP.
OrthoDBiEOG79SDWX.
TreeFamiTF317925.

Family and domain databases

InterProiIPR002159. CD36.
IPR005428. CD36_antigen.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01610. CD36ANTIGEN.
PR01609. CD36FAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCNRNCGLI AGAVIGAVLA VFGGILMPVG DMLIEKTIKK EVVLEEGTIA
60 70 80 90 100
FKNWVKTGTD VYRQFWIFDV QNPDEVTVNS SKIKVKQRGP YTYRVRYLAK
110 120 130 140 150
ENITQDPETH TVSFLQPNGA IFEPSLSVGT EDDTFTILNL AVAAAPQLYP
160 170 180 190 200
NTFMQGILNS FIKKSKSSMF QNRTLKELLW GYTDPFLNLV PYPITTTIGV
210 220 230 240 250
FYPYNNTADG IYKVFNGKDD ISKVAIIDTY KGRKNLSYWS SYCDLINGTD
260 270 280 290 300
AASFPPFVEK TRVLQFFSSD ICRSIYAVFG AEINLKGIPV YRFILPSFAF
310 320 330 340 350
ASPFQNPDNH CFCTEKIISK NCTLYGVLDI GKCKEGKPVY ISLPHFLHGS
360 370 380 390 400
PELAEPIESL SPNEEEHSTY LDVEPITGFT LRFAKRLQVN MLVKPAKKIE
410 420 430 440 450
ALKNLKHNYI VPILWLNETG TIGDEKAEMF RNQVTGKINL LGLVEIVLLS
460 470
VGVVMFIAFM ISYCACRSKR VN
Length:472
Mass (Da):52,940
Last modified:January 23, 2007 - v5
Checksum:iD864D6D3D6E8622F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391K → N in CAA62803 (PubMed:8950178).Curated
Sequence conflicti110 – 1101H → N in BAA08224 (Ref. 1) Curated
Sequence conflicti125 – 1251S → L in BAA08224 (Ref. 1) Curated
Sequence conflicti134 – 1341T → K in BAA08224 (Ref. 1) Curated
Sequence conflicti257 – 2571F → L in BAA08224 (Ref. 1) Curated
Sequence conflicti294 – 2941I → V in BAA08224 (Ref. 1) Curated
Sequence conflicti305 – 3051Q → E in BAA08224 (Ref. 1) Curated
Sequence conflicti359 – 3591S → G in BAA08224 (Ref. 1) Curated
Sequence conflicti391 – 3966MLVKPA → TGQARQ in BAA08224 (Ref. 1) Curated
Sequence conflicti466 – 4661C → Y in BAA08224 (Ref. 1) Curated
Sequence conflicti470 – 4723RVN → TIK in BAA08224 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45364 mRNA. Translation: BAA08224.1.
X91503 mRNA. Translation: CAA62803.1.
BC103112 mRNA. Translation: AAI03113.1.
RefSeqiNP_001265550.1. NM_001278621.1.
NP_776435.2. NM_174010.3.
XP_005205381.1. XM_005205324.2.
XP_005205382.1. XM_005205325.2.
XP_005205383.1. XM_005205326.2.
XP_010802454.1. XM_010804152.1.
XP_010802455.1. XM_010804153.1.
XP_010802457.1. XM_010804155.1.
XP_010802458.1. XM_010804156.1.
XP_010802459.1. XM_010804157.1.
UniGeneiBt.43948.

Genome annotation databases

EnsembliENSBTAT00000023750; ENSBTAP00000023750; ENSBTAG00000017866.
GeneIDi281052.
KEGGibta:281052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45364 mRNA. Translation: BAA08224.1.
X91503 mRNA. Translation: CAA62803.1.
BC103112 mRNA. Translation: AAI03113.1.
RefSeqiNP_001265550.1. NM_001278621.1.
NP_776435.2. NM_174010.3.
XP_005205381.1. XM_005205324.2.
XP_005205382.1. XM_005205325.2.
XP_005205383.1. XM_005205326.2.
XP_010802454.1. XM_010804152.1.
XP_010802455.1. XM_010804153.1.
XP_010802457.1. XM_010804155.1.
XP_010802458.1. XM_010804156.1.
XP_010802459.1. XM_010804157.1.
UniGeneiBt.43948.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000023750.

PTM databases

UniCarbKBiP26201.

Proteomic databases

PaxDbiP26201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023750; ENSBTAP00000023750; ENSBTAG00000017866.
GeneIDi281052.
KEGGibta:281052.

Organism-specific databases

CTDi948.

Phylogenomic databases

eggNOGiNOG257244.
GeneTreeiENSGT00530000062927.
HOGENOMiHOG000252951.
HOVERGENiHBG002754.
InParanoidiP26201.
KOiK06259.
OMAiKRNYIVP.
OrthoDBiEOG79SDWX.
TreeFamiTF317925.

Enzyme and pathway databases

ReactomeiREACT_272065. Platelet degranulation.
REACT_303007. Toll Like Receptor TLR6:TLR2 Cascade.
REACT_305366. MyD88:Mal cascade initiated on plasma membrane.
REACT_318860. Cross-presentation of particulate exogenous antigens (phagosomes).
REACT_353291. Scavenging by Class B Receptors.

Miscellaneous databases

NextBioi20805136.

Family and domain databases

InterProiIPR002159. CD36.
IPR005428. CD36_antigen.
[Graphical view]
PANTHERiPTHR11923. PTHR11923. 1 hit.
PfamiPF01130. CD36. 1 hit.
[Graphical view]
PRINTSiPR01610. CD36ANTIGEN.
PR01609. CD36FAMILY.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of PAS-4 from bovine fat globule membrane."
    Hwangbo S., Ametani M., Kanno C.
    Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Holstein.
    Tissue: Mammary gland.
  2. "Structural characterization of bovine CD36 from the milk fat globule membrane."
    Berglund L., Petersen T.E., Ramussen J.T.
    Biochim. Biophys. Acta 1309:63-68(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION AT ASN-79; ASN-102; ASN-172; ASN-205; ASN-235; ASN-247; ASN-321 AND ASN-417.
    Tissue: Mammary gland.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Heart ventricle.
  4. "Structural, functional, and antigenic differences between bovine heart endothelial CD36 and human platelet CD36."
    Greenwalt D.E., Watt K.W.K., Hasler T., Howard R.J., Patel S.
    J. Biol. Chem. 265:16296-16299(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-22, FUNCTION.
    Tissue: Heart.
  5. "PAS IV, an integral membrane protein of mammary epithelial cells, is related to platelet and endothelial cell CD36 (GP IV)."
    Greenwalt D.E., Watt K.W., So O.Y., Jiwani N.
    Biochemistry 29:7054-7059(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 4-22 AND 214-223.
    Tissue: Mammary epithelium.
  6. Cited for: DISULFIDE BONDS.
    Tissue: Milk.

Entry informationi

Entry nameiCD36_BOVIN
AccessioniPrimary (citable) accession number: P26201
Secondary accession number(s): P79111, Q28154, Q3ZBT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 112 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.