SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P26196

- DDX6_HUMAN

UniProt

P26196 - DDX6_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Probable ATP-dependent RNA helicase DDX6
Gene
DDX6, HLR2, RCK
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

In the process of mRNA degradation, may play a role in mRNA decapping.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 1478ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. RNA helicase activity Source: ProtInc
  4. helicase activity Source: ProtInc
  5. poly(A) RNA binding Source: UniProtKB
  6. protein binding Source: UniProtKB

GO - Biological processi

  1. RNA metabolic process Source: Reactome
  2. cytoplasmic mRNA processing body assembly Source: MGI
  3. exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay Source: Reactome
  4. gene expression Source: Reactome
  5. mRNA metabolic process Source: Reactome
  6. nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_20518. mRNA decay by 5' to 3' exoribonuclease.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX6 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase p54
DEAD box protein 6
Oncogene RCK
Gene namesi
Name:DDX6
Synonyms:HLR2, RCK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:2747. DDX6.

Subcellular locationi

CytoplasmP-body 4 Publications

GO - Cellular componenti

  1. RISC complex Source: MGI
  2. cytoplasm Source: HPA
  3. cytoplasmic mRNA processing body Source: UniProtKB
  4. cytoplasmic stress granule Source: UniProtKB
  5. cytosol Source: Reactome
  6. intracellular membrane-bounded organelle Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi320 – 3201Q → A: Abolishes interaction with EDC3; when associated with A-323; A-327 and A-331. 1 Publication
Mutagenesisi323 – 3231H → A: Abolishes interaction with EDC3; when associated with A-320; A-327 and A-331. 1 Publication
Mutagenesisi327 – 3271T → A: Abolishes interaction with EDC3; when associated with A-320; A-323 and A-331. 1 Publication
Mutagenesisi331 – 3311R → A: Abolishes interaction with EDC3; when associated with A-320; A-323 and A-327. 1 Publication

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA27229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Probable ATP-dependent RNA helicase DDX6
PRO_0000054983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP26196.
PaxDbiP26196.
PeptideAtlasiP26196.
PRIDEiP26196.

2D gel databases

DOSAC-COBS-2DPAGEP26196.

PTM databases

PhosphoSiteiP26196.

Expressioni

Tissue specificityi

Abundantly expressed in most tissues.

Gene expression databases

ArrayExpressiP26196.
BgeeiP26196.
CleanExiHS_DDX6.
GenevestigatoriP26196.

Organism-specific databases

HPAiCAB004668.
HPA024201.
HPA026644.

Interactioni

Subunit structurei

Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with RC3H1 By similarity.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATXN2Q997007EBI-351257,EBI-697691
DCP1AQ9NPI64EBI-351257,EBI-374238
EDC3Q96F862EBI-351257,EBI-997311

Protein-protein interaction databases

BioGridi108022. 42 interactions.
DIPiDIP-29195N.
IntActiP26196. 31 interactions.
MINTiMINT-5004149.
STRINGi9606.ENSP00000264018.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi98 – 1003
Helixi105 – 1128
Turni113 – 1153
Helixi121 – 13111
Beta strandi136 – 1394
Beta strandi142 – 1443
Helixi147 – 15711
Beta strandi167 – 1704
Helixi174 – 18714
Turni188 – 1903
Beta strandi191 – 1933
Beta strandi196 – 1994
Beta strandi201 – 2033
Helixi205 – 2117
Beta strandi217 – 2215
Helixi223 – 2319
Beta strandi242 – 2476
Helixi248 – 2514
Turni254 – 2563
Helixi257 – 26610
Beta strandi272 – 2787
Helixi282 – 29110
Beta strandi296 – 2983
Beta strandi309 – 3157
Helixi318 – 3203
Helixi321 – 33111
Beta strandi335 – 3406
Helixi344 – 35613
Beta strandi361 – 3644
Beta strandi366 – 3683
Helixi370 – 38112
Beta strandi384 – 3907
Helixi397 – 3993
Beta strandi403 – 4108
Helixi415 – 4228
Helixi423 – 4253
Beta strandi432 – 4387
Helixi440 – 4423
Helixi443 – 45311
Helixi462 – 4654
Helixi466 – 4683

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VECX-ray2.01A/B94-299[»]
2WAXX-ray2.30A/C296-483[»]
2WAYX-ray2.30A/C296-483[»]
4CRWX-ray1.75B307-483[»]
4CT4X-ray2.30B/D95-469[»]
4CT5X-ray3.00A/B95-469[»]
ProteinModelPortaliP26196.
SMRiP26196. Positions 63-472.

Miscellaneous databases

EvolutionaryTraceiP26196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini127 – 298172Helicase ATP-binding
Add
BLAST
Domaini308 – 468161Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi96 – 12429Q motif
Add
BLAST
Motifi246 – 2494DEAD box

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0513.
HOGENOMiHOG000268797.
HOVERGENiHBG106685.
InParanoidiP26196.
KOiK12614.
OMAiLLQFHPK.
OrthoDBiEOG7D85W7.
PhylomeDBiP26196.
TreeFamiTF300440.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26196-1 [UniParc]FASTAAdd to Basket

« Hide

MSTARTENPV IMGLSSQNGQ LRGPVKPTGG PGGGGTQTQQ QMNQLKNTNT    50
INNGTQQQAQ SMTTTIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED 100
YCLKRELLMG IFEMGWEKPS PIQEESIPIA LSGRDILARA KNGTGKSGAY 150
LIPLLERLDL KKDNIQAMVI VPTRELALQV SQICIQVSKH MGGAKVMATT 200
GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAKVDHV QMIVLDEADK 250
LLSQDFVQIM EDIILTLPKN RQILLYSATF PLSVQKFMNS HLQKPYEINL 300
MEELTLKGVT QYYAYVTERQ KVHCLNTLFS RLQINQSIIF CNSSQRVELL 350
AKKISQLGYS CFYIHAKMRQ EHRNRVFHDF RNGLCRNLVC TDLFTRGIDI 400
QAVNVVINFD FPKLAETYLH RIGRSGRFGH LGLAINLITY DDRFNLKSIE 450
EQLGTEIKPI PSNIDKSLYV AEYHSEPVED EKP 483
Length:483
Mass (Da):54,417
Last modified:October 17, 2006 - v2
Checksum:iF802C642861793FB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931Q → E in Z11685. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11685 mRNA. No translation available.
BC065007 mRNA. Translation: AAH65007.1.
D17532 mRNA. Translation: BAA04482.1.
CCDSiCCDS44751.1.
PIRiS22651.
RefSeqiNP_001244120.1. NM_001257191.2.
NP_004388.2. NM_004397.5.
XP_005271474.1. XM_005271417.2.
UniGeneiHs.408461.

Genome annotation databases

EnsembliENST00000264018; ENSP00000264018; ENSG00000110367.
ENST00000526070; ENSP00000433704; ENSG00000110367.
ENST00000534980; ENSP00000442266; ENSG00000110367.
ENST00000593895; ENSP00000472890; ENSG00000269612.
ENST00000596625; ENSP00000471023; ENSG00000269612.
ENST00000600727; ENSP00000469200; ENSG00000269612.
GeneIDi1656.
KEGGihsa:1656.
UCSCiuc001pub.2. human.

Polymorphism databases

DMDMi116241327.

Keywords - Coding sequence diversityi

Chromosomal rearrangement

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11685 mRNA. No translation available.
BC065007 mRNA. Translation: AAH65007.1 .
D17532 mRNA. Translation: BAA04482.1 .
CCDSi CCDS44751.1.
PIRi S22651.
RefSeqi NP_001244120.1. NM_001257191.2.
NP_004388.2. NM_004397.5.
XP_005271474.1. XM_005271417.2.
UniGenei Hs.408461.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VEC X-ray 2.01 A/B 94-299 [» ]
2WAX X-ray 2.30 A/C 296-483 [» ]
2WAY X-ray 2.30 A/C 296-483 [» ]
4CRW X-ray 1.75 B 307-483 [» ]
4CT4 X-ray 2.30 B/D 95-469 [» ]
4CT5 X-ray 3.00 A/B 95-469 [» ]
ProteinModelPortali P26196.
SMRi P26196. Positions 63-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108022. 42 interactions.
DIPi DIP-29195N.
IntActi P26196. 31 interactions.
MINTi MINT-5004149.
STRINGi 9606.ENSP00000264018.

PTM databases

PhosphoSitei P26196.

Polymorphism databases

DMDMi 116241327.

2D gel databases

DOSAC-COBS-2DPAGE P26196.

Proteomic databases

MaxQBi P26196.
PaxDbi P26196.
PeptideAtlasi P26196.
PRIDEi P26196.

Protocols and materials databases

DNASUi 1656.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000264018 ; ENSP00000264018 ; ENSG00000110367 .
ENST00000526070 ; ENSP00000433704 ; ENSG00000110367 .
ENST00000534980 ; ENSP00000442266 ; ENSG00000110367 .
ENST00000593895 ; ENSP00000472890 ; ENSG00000269612 .
ENST00000596625 ; ENSP00000471023 ; ENSG00000269612 .
ENST00000600727 ; ENSP00000469200 ; ENSG00000269612 .
GeneIDi 1656.
KEGGi hsa:1656.
UCSCi uc001pub.2. human.

Organism-specific databases

CTDi 1656.
GeneCardsi GC11M118619.
HGNCi HGNC:2747. DDX6.
HPAi CAB004668.
HPA024201.
HPA026644.
MIMi 600326. gene.
neXtProti NX_P26196.
PharmGKBi PA27229.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0513.
HOGENOMi HOG000268797.
HOVERGENi HBG106685.
InParanoidi P26196.
KOi K12614.
OMAi LLQFHPK.
OrthoDBi EOG7D85W7.
PhylomeDBi P26196.
TreeFami TF300440.

Enzyme and pathway databases

Reactomei REACT_20518. mRNA decay by 5' to 3' exoribonuclease.

Miscellaneous databases

ChiTaRSi DDX6. human.
EvolutionaryTracei P26196.
GeneWikii DDX6.
GenomeRNAii 1656.
NextBioi 6820.
PROi P26196.
SOURCEi Search...

Gene expression databases

ArrayExpressi P26196.
Bgeei P26196.
CleanExi HS_DDX6.
Genevestigatori P26196.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and localization of an RNA helicase gene from a human lymphoid cell line with chromosomal breakpoint 11q23.3."
    Lu D., Yunis J.J.
    Nucleic Acids Res. 20:1967-1972(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  3. "The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocations."
    Akao Y., Seto M., Yamamoto K., Iida S., Nakazawa S., Inazawa J., Abe T., Takahashi T., Ueda R.
    Cancer Res. 52:6083-6087(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-483.
    Tissue: Lung.
  4. "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping."
    Fenger-Groen M., Fillman C., Norrild B., Lykke-Andersen J.
    Mol. Cell 20:905-915(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH DCP1A; DCP2; EDC3 AND EDC4, SUBCELLULAR LOCATION.
  5. "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies."
    Wichroski M.J., Robb G.B., Rana T.M.
    PLoS Pathog. 2:E41-E41(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH APOBEC3G.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  7. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LIMD1; WTIP AND AJUBA.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies."
    Phalora P.K., Sherer N.M., Wolinsky S.M., Swanson C.M., Malim M.H.
    J. Virol. 86:11712-11724(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Crystallization and X-ray analysis of the N-terminal core domain of a tumour-associated human DEAD-box RNA helicase, rck/p54."
    Matsui T., Hogetsu K., Akao Y., Tanaka M., Sato T., Kumasaka T., Tanaka N.
    Acta Crystallogr. D 60:156-159(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 94-299.
  11. "Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B."
    Tritschler F., Braun J.E., Eulalio A., Truffault V., Izaurralde E., Weichenrieder O.
    Mol. Cell 33:661-668(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 296-483 IN COMPLEX WITH EDC3, MUTAGENESIS OF GLN-320; HIS-323; THR-327 AND ARG-331, INTERACTION WITH EDC3.

Entry informationi

Entry nameiDDX6_HUMAN
AccessioniPrimary (citable) accession number: P26196
Secondary accession number(s): Q5D048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi