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P26196

- DDX6_HUMAN

UniProt

P26196 - DDX6_HUMAN

Protein

Probable ATP-dependent RNA helicase DDX6

Gene

DDX6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 156 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    In the process of mRNA degradation, may play a role in mRNA decapping.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi140 – 1478ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. helicase activity Source: ProtInc
    4. poly(A) RNA binding Source: UniProtKB
    5. protein binding Source: UniProtKB
    6. RNA helicase activity Source: ProtInc

    GO - Biological processi

    1. cytoplasmic mRNA processing body assembly Source: MGI
    2. exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay Source: Reactome
    3. gene expression Source: Reactome
    4. mRNA metabolic process Source: Reactome
    5. nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: Reactome
    6. RNA metabolic process Source: Reactome

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_20518. mRNA decay by 5' to 3' exoribonuclease.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX6 (EC:3.6.4.13)
    Alternative name(s):
    ATP-dependent RNA helicase p54
    DEAD box protein 6
    Oncogene RCK
    Gene namesi
    Name:DDX6
    Synonyms:HLR2, RCK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:2747. DDX6.

    Subcellular locationi

    CytoplasmP-body 4 Publications

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytoplasmic mRNA processing body Source: UniProtKB
    3. cytoplasmic stress granule Source: UniProtKB
    4. cytosol Source: Reactome
    5. intracellular membrane-bounded organelle Source: HPA
    6. membrane Source: UniProtKB
    7. RISC complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving DDX6 may be a cause of hematopoietic tumors such as B-cell lymphomas. Translocation t(11;14)(q23;q32).

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi320 – 3201Q → A: Abolishes interaction with EDC3; when associated with A-323; A-327 and A-331. 1 Publication
    Mutagenesisi323 – 3231H → A: Abolishes interaction with EDC3; when associated with A-320; A-327 and A-331. 1 Publication
    Mutagenesisi327 – 3271T → A: Abolishes interaction with EDC3; when associated with A-320; A-323 and A-331. 1 Publication
    Mutagenesisi331 – 3311R → A: Abolishes interaction with EDC3; when associated with A-320; A-323 and A-327. 1 Publication

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    PharmGKBiPA27229.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Probable ATP-dependent RNA helicase DDX6PRO_0000054983Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP26196.
    PaxDbiP26196.
    PeptideAtlasiP26196.
    PRIDEiP26196.

    2D gel databases

    DOSAC-COBS-2DPAGEP26196.

    PTM databases

    PhosphoSiteiP26196.

    Expressioni

    Tissue specificityi

    Abundantly expressed in most tissues.

    Gene expression databases

    ArrayExpressiP26196.
    BgeeiP26196.
    CleanExiHS_DDX6.
    GenevestigatoriP26196.

    Organism-specific databases

    HPAiCAB004668.
    HPA024201.
    HPA026644.

    Interactioni

    Subunit structurei

    Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with RC3H1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATXN2Q997007EBI-351257,EBI-697691
    DCP1AQ9NPI65EBI-351257,EBI-374238
    EDC3Q96F862EBI-351257,EBI-997311

    Protein-protein interaction databases

    BioGridi108022. 42 interactions.
    DIPiDIP-29195N.
    IntActiP26196. 32 interactions.
    MINTiMINT-5004149.
    STRINGi9606.ENSP00000264018.

    Structurei

    Secondary structure

    1
    483
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi98 – 1003
    Helixi105 – 1128
    Turni113 – 1153
    Helixi121 – 13111
    Beta strandi136 – 1394
    Beta strandi142 – 1443
    Helixi147 – 15711
    Beta strandi167 – 1704
    Helixi174 – 18714
    Turni188 – 1903
    Beta strandi191 – 1933
    Beta strandi196 – 1994
    Beta strandi201 – 2033
    Helixi205 – 2117
    Beta strandi217 – 2215
    Helixi223 – 2319
    Beta strandi242 – 2476
    Helixi248 – 2514
    Turni254 – 2563
    Helixi257 – 26610
    Beta strandi272 – 2787
    Helixi282 – 29110
    Beta strandi296 – 2983
    Beta strandi309 – 3157
    Helixi318 – 3203
    Helixi321 – 33111
    Beta strandi335 – 3406
    Helixi344 – 35613
    Beta strandi361 – 3644
    Beta strandi366 – 3683
    Helixi370 – 38112
    Beta strandi384 – 3907
    Helixi397 – 3993
    Beta strandi403 – 4108
    Helixi415 – 4228
    Helixi423 – 4253
    Beta strandi432 – 4387
    Helixi440 – 4423
    Helixi443 – 45311
    Helixi462 – 4654
    Helixi466 – 4683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VECX-ray2.01A/B94-299[»]
    2WAXX-ray2.30A/C296-483[»]
    2WAYX-ray2.30A/C296-483[»]
    4CRWX-ray1.75B307-483[»]
    4CT4X-ray2.30B/D95-469[»]
    4CT5X-ray3.00A/B95-469[»]
    ProteinModelPortaliP26196.
    SMRiP26196. Positions 63-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26196.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini127 – 298172Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini308 – 468161Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi96 – 12429Q motifAdd
    BLAST
    Motifi246 – 2494DEAD box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOGENOMiHOG000268797.
    HOVERGENiHBG106685.
    InParanoidiP26196.
    KOiK12614.
    OMAiLLQFHPK.
    OrthoDBiEOG7D85W7.
    PhylomeDBiP26196.
    TreeFamiTF300440.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26196-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTARTENPV IMGLSSQNGQ LRGPVKPTGG PGGGGTQTQQ QMNQLKNTNT    50
    INNGTQQQAQ SMTTTIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED 100
    YCLKRELLMG IFEMGWEKPS PIQEESIPIA LSGRDILARA KNGTGKSGAY 150
    LIPLLERLDL KKDNIQAMVI VPTRELALQV SQICIQVSKH MGGAKVMATT 200
    GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAKVDHV QMIVLDEADK 250
    LLSQDFVQIM EDIILTLPKN RQILLYSATF PLSVQKFMNS HLQKPYEINL 300
    MEELTLKGVT QYYAYVTERQ KVHCLNTLFS RLQINQSIIF CNSSQRVELL 350
    AKKISQLGYS CFYIHAKMRQ EHRNRVFHDF RNGLCRNLVC TDLFTRGIDI 400
    QAVNVVINFD FPKLAETYLH RIGRSGRFGH LGLAINLITY DDRFNLKSIE 450
    EQLGTEIKPI PSNIDKSLYV AEYHSEPVED EKP 483
    Length:483
    Mass (Da):54,417
    Last modified:October 17, 2006 - v2
    Checksum:iF802C642861793FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti293 – 2931Q → E in Z11685. (PubMed:1579499)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11685 mRNA. No translation available.
    BC065007 mRNA. Translation: AAH65007.1.
    D17532 mRNA. Translation: BAA04482.1.
    CCDSiCCDS44751.1.
    PIRiS22651.
    RefSeqiNP_001244120.1. NM_001257191.2.
    NP_004388.2. NM_004397.5.
    XP_005271474.1. XM_005271417.2.
    UniGeneiHs.408461.

    Genome annotation databases

    EnsembliENST00000526070; ENSP00000433704; ENSG00000110367.
    ENST00000534980; ENSP00000442266; ENSG00000110367.
    GeneIDi1656.
    KEGGihsa:1656.
    UCSCiuc001pub.2. human.

    Polymorphism databases

    DMDMi116241327.

    Keywords - Coding sequence diversityi

    Chromosomal rearrangement

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11685 mRNA. No translation available.
    BC065007 mRNA. Translation: AAH65007.1 .
    D17532 mRNA. Translation: BAA04482.1 .
    CCDSi CCDS44751.1.
    PIRi S22651.
    RefSeqi NP_001244120.1. NM_001257191.2.
    NP_004388.2. NM_004397.5.
    XP_005271474.1. XM_005271417.2.
    UniGenei Hs.408461.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VEC X-ray 2.01 A/B 94-299 [» ]
    2WAX X-ray 2.30 A/C 296-483 [» ]
    2WAY X-ray 2.30 A/C 296-483 [» ]
    4CRW X-ray 1.75 B 307-483 [» ]
    4CT4 X-ray 2.30 B/D 95-469 [» ]
    4CT5 X-ray 3.00 A/B 95-469 [» ]
    ProteinModelPortali P26196.
    SMRi P26196. Positions 63-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108022. 42 interactions.
    DIPi DIP-29195N.
    IntActi P26196. 32 interactions.
    MINTi MINT-5004149.
    STRINGi 9606.ENSP00000264018.

    PTM databases

    PhosphoSitei P26196.

    Polymorphism databases

    DMDMi 116241327.

    2D gel databases

    DOSAC-COBS-2DPAGE P26196.

    Proteomic databases

    MaxQBi P26196.
    PaxDbi P26196.
    PeptideAtlasi P26196.
    PRIDEi P26196.

    Protocols and materials databases

    DNASUi 1656.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000526070 ; ENSP00000433704 ; ENSG00000110367 .
    ENST00000534980 ; ENSP00000442266 ; ENSG00000110367 .
    GeneIDi 1656.
    KEGGi hsa:1656.
    UCSCi uc001pub.2. human.

    Organism-specific databases

    CTDi 1656.
    GeneCardsi GC11M118619.
    HGNCi HGNC:2747. DDX6.
    HPAi CAB004668.
    HPA024201.
    HPA026644.
    MIMi 600326. gene.
    neXtProti NX_P26196.
    PharmGKBi PA27229.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0513.
    HOGENOMi HOG000268797.
    HOVERGENi HBG106685.
    InParanoidi P26196.
    KOi K12614.
    OMAi LLQFHPK.
    OrthoDBi EOG7D85W7.
    PhylomeDBi P26196.
    TreeFami TF300440.

    Enzyme and pathway databases

    Reactomei REACT_20518. mRNA decay by 5' to 3' exoribonuclease.

    Miscellaneous databases

    ChiTaRSi DDX6. human.
    EvolutionaryTracei P26196.
    GeneWikii DDX6.
    GenomeRNAii 1656.
    NextBioi 6820.
    PROi P26196.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P26196.
    Bgeei P26196.
    CleanExi HS_DDX6.
    Genevestigatori P26196.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression and localization of an RNA helicase gene from a human lymphoid cell line with chromosomal breakpoint 11q23.3."
      Lu D., Yunis J.J.
      Nucleic Acids Res. 20:1967-1972(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    3. "The RCK gene associated with t(11;14) translocation is distinct from the MLL/ALL-1 gene with t(4;11) and t(11;19) translocations."
      Akao Y., Seto M., Yamamoto K., Iida S., Nakazawa S., Inazawa J., Abe T., Takahashi T., Ueda R.
      Cancer Res. 52:6083-6087(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-483.
      Tissue: Lung.
    4. "Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping."
      Fenger-Groen M., Fillman C., Norrild B., Lykke-Andersen J.
      Mol. Cell 20:905-915(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH DCP1A; DCP2; EDC3 AND EDC4, SUBCELLULAR LOCATION.
    5. "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies."
      Wichroski M.J., Robb G.B., Rana T.M.
      PLoS Pathog. 2:E41-E41(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH APOBEC3G.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    7. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH LIMD1; WTIP AND AJUBA.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies."
      Phalora P.K., Sherer N.M., Wolinsky S.M., Swanson C.M., Malim M.H.
      J. Virol. 86:11712-11724(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Crystallization and X-ray analysis of the N-terminal core domain of a tumour-associated human DEAD-box RNA helicase, rck/p54."
      Matsui T., Hogetsu K., Akao Y., Tanaka M., Sato T., Kumasaka T., Tanaka N.
      Acta Crystallogr. D 60:156-159(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 94-299.
    11. "Structural basis for the mutually exclusive anchoring of P body components EDC3 and Tral to the DEAD box protein DDX6/Me31B."
      Tritschler F., Braun J.E., Eulalio A., Truffault V., Izaurralde E., Weichenrieder O.
      Mol. Cell 33:661-668(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 296-483 IN COMPLEX WITH EDC3, MUTAGENESIS OF GLN-320; HIS-323; THR-327 AND ARG-331, INTERACTION WITH EDC3.

    Entry informationi

    Entry nameiDDX6_HUMAN
    AccessioniPrimary (citable) accession number: P26196
    Secondary accession number(s): Q5D048
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 156 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3