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P26193

- VP4_ROTPC

UniProt

P26193 - VP4_ROTPC

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Protein
Outer capsid protein VP4
Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.
VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei231 – 2322Cleavage Reviewed prediction
Sitei247 – 2482Cleavage Reviewed prediction

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008175: Genome

Subcellular locationi

Chain Outer capsid protein VP4 : Virion. Host rough endoplasmic reticulum Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.
Chain Outer capsid protein VP8* : Virion By similarity
Note: Outer capsid protein By similarity.
Chain Outer capsid protein VP5* : Virion By similarity
Note: Outer capsid protein By similarity.

GO - Cellular componenti

  1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736Outer capsid protein VP4
PRO_0000041099Add
BLAST
Chaini1 – 231231Outer capsid protein VP8* Reviewed prediction
PRO_0000041100Add
BLAST
Chaini247 – 736490Outer capsid protein VP5* Reviewed prediction
PRO_0000041101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi61 – 611N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi64 – 641N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi93 – 931N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi162 – 1621N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi191 – 1911N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi237 – 2371N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi251 – 2511N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi304 – 3041N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi471 – 4711N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi631 – 6311N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer Reviewed prediction.

Structurei

3D structure databases

ProteinModelPortaliP26193.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni393 – 41321Hydrophobic; possible role in virus entry into host cell Reviewed prediction
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili488 – 52235 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus VP4 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26193-1 [UniParc]FASTAAdd to Basket

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MASSLYQQLI SQNYYSIGNE ILTDQQTTET VVDYVDAGNY TYAQLPPTKW    50
GARGTFKSAF NVSNITGPHT NTIIEWSNLL NSNGWVIYQK PANTTKLFKH 100
GPETYNSNLA AFELWYGKAG TSVTSDYYSS LQNNEKTVTA TSDSLILFWN 150
EGSTVLANKK VNFSWDMGGM LIKPTRGNRV DICMANMNDF NSSIFNWEEW 200
KHEFPRSDVN INVNMYTDYY LASEDPYTEL KALQQPNITT FEMKMMKIIR 250
NGSINLNEVV SKDSLWQEVR YARDITLECK IESEVVKGGG WGYDYTSVAF 300
KTVNHTYTYT RAGEIVNAHV TISFNNMKER SYGGSLPTDF KIGRFDVIDT 350
DTYMYIDYWD DSEIFKNMVY VRDLSANIGG FFYYAEMSYY FQIPVGAHPG 400
LHSSGVRFVY ERCLLSQQFT DQVALNSMRF IFRVTESNGW FMTSGNINTR 450
RIASGTGFAY ADGHTSQTVG NITFISLIPS NPNYQTPIAS SSTVRMDLER 500
KINDLRNDFN QLANSVALGD ILSLATSPLT FANLLESVPA IASSVKDVAA 550
NVMKKFRNTK MFKKATKAKY SEFIIGDLLE DVTNVARNSN GMNFDDITSA 600
VMVSTTNKLQ LTDVDTLSEI VARSADNFIP NRSYRMIEDG IVYEATPKRT 650
FSYDLTTLQQ REFDIDKFMR LASKSPVISA IVDFATLKAM RETYGVGTDV 700
IYKLVASDAP TILSFIDNNN PLIKSRIEEL LRQCRL 736
Length:736
Mass (Da):83,232
Last modified:May 1, 1992 - v1
Checksum:i356F9226D5016577
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74218 Genomic DNA. Translation: AAB00802.1.
PIRiD40822. VPXRPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74218 Genomic DNA. Translation: AAB00802.1 .
PIRi D40822. VPXRPC.

3D structure databases

ProteinModelPortali P26193.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000416. Haemagglutinin_VP4.
[Graphical view ]
Pfami PF00426. VP4_haemagglut. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
    Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
    Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiVP4_ROTPC
AccessioniPrimary (citable) accession number: P26193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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