Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P26193

- VP4_ROTPC

UniProt

P26193 - VP4_ROTPC

Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.By similarity
    Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.By similarity
    VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei231 – 2322CleavageSequence Analysis
    Sitei247 – 2482CleavageSequence Analysis

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein VP4
    Alternative name(s):
    Hemagglutinin
    Cleaved into the following 2 chains:
    OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
    Taxonomic identifieri10916 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008175: Genome

    Subcellular locationi

    Chain Outer capsid protein VP4 : Virion. Host rough endoplasmic reticulum Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity
    Chain Outer capsid protein VP8* : Virion By similarity
    Note: Outer capsid protein.By similarity
    Chain Outer capsid protein VP5* : Virion By similarity
    Note: Outer capsid protein.By similarity

    GO - Cellular componenti

    1. host cell rough endoplasmic reticulum Source: UniProtKB-SubCell
    2. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 736736Outer capsid protein VP4PRO_0000041099Add
    BLAST
    Chaini1 – 231231Outer capsid protein VP8*Sequence AnalysisPRO_0000041100Add
    BLAST
    Chaini247 – 736490Outer capsid protein VP5*Sequence AnalysisPRO_0000041101Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi61 – 611N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi64 – 641N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi93 – 931N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi191 – 1911N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi237 – 2371N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi251 – 2511N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi304 – 3041N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi471 – 4711N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi631 – 6311N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    VP4 is a homotrimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliP26193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni393 – 41321Hydrophobic; possible role in virus entry into host cellSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili488 – 52235Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus VP4 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR000416. Haemagglutinin_VP4.
    [Graphical view]
    PfamiPF00426. VP4_haemagglut. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26193-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSLYQQLI SQNYYSIGNE ILTDQQTTET VVDYVDAGNY TYAQLPPTKW    50
    GARGTFKSAF NVSNITGPHT NTIIEWSNLL NSNGWVIYQK PANTTKLFKH 100
    GPETYNSNLA AFELWYGKAG TSVTSDYYSS LQNNEKTVTA TSDSLILFWN 150
    EGSTVLANKK VNFSWDMGGM LIKPTRGNRV DICMANMNDF NSSIFNWEEW 200
    KHEFPRSDVN INVNMYTDYY LASEDPYTEL KALQQPNITT FEMKMMKIIR 250
    NGSINLNEVV SKDSLWQEVR YARDITLECK IESEVVKGGG WGYDYTSVAF 300
    KTVNHTYTYT RAGEIVNAHV TISFNNMKER SYGGSLPTDF KIGRFDVIDT 350
    DTYMYIDYWD DSEIFKNMVY VRDLSANIGG FFYYAEMSYY FQIPVGAHPG 400
    LHSSGVRFVY ERCLLSQQFT DQVALNSMRF IFRVTESNGW FMTSGNINTR 450
    RIASGTGFAY ADGHTSQTVG NITFISLIPS NPNYQTPIAS SSTVRMDLER 500
    KINDLRNDFN QLANSVALGD ILSLATSPLT FANLLESVPA IASSVKDVAA 550
    NVMKKFRNTK MFKKATKAKY SEFIIGDLLE DVTNVARNSN GMNFDDITSA 600
    VMVSTTNKLQ LTDVDTLSEI VARSADNFIP NRSYRMIEDG IVYEATPKRT 650
    FSYDLTTLQQ REFDIDKFMR LASKSPVISA IVDFATLKAM RETYGVGTDV 700
    IYKLVASDAP TILSFIDNNN PLIKSRIEEL LRQCRL 736
    Length:736
    Mass (Da):83,232
    Last modified:May 1, 1992 - v1
    Checksum:i356F9226D5016577
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74218 Genomic DNA. Translation: AAB00802.1.
    PIRiD40822. VPXRPC.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74218 Genomic DNA. Translation: AAB00802.1 .
    PIRi D40822. VPXRPC.

    3D structure databases

    ProteinModelPortali P26193.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000416. Haemagglutinin_VP4.
    [Graphical view ]
    Pfami PF00426. VP4_haemagglut. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
      Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
      Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiVP4_ROTPC
    AccessioniPrimary (citable) accession number: P26193
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3