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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus C (strain RVC/Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors (By similarity).By similarity
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm (By similarity).By similarity
VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus C (strain RVC/Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000008175 Componenti: Genome

Subcellular locationi

Outer capsid protein VP4 :
  • Virion
  • Host rough endoplasmic reticulum Curated

  • Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity
Outer capsid protein VP8* :
  • Virion By similarity

  • Note: Outer capsid protein.By similarity
Outer capsid protein VP5* :
  • Virion By similarity

  • Note: Outer capsid protein.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000410991 – 736Outer capsid protein VP4Add BLAST736
ChainiPRO_00000411001 – 231Outer capsid protein VP8*Sequence analysisAdd BLAST231
ChainiPRO_0000041101247 – 736Outer capsid protein VP5*Sequence analysisAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi61N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi64N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi93N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi162N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi191N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi237N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi251N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi304N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi471N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi631N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei231 – 232CleavageSequence analysis2
Sitei247 – 248CleavageSequence analysis2

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer.Curated

Structurei

3D structure databases

ProteinModelPortaliP26193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni393 – 413Hydrophobic; possible role in virus entry into host cellSequence analysisAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili488 – 522Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the rotavirus VP4 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLYQQLI SQNYYSIGNE ILTDQQTTET VVDYVDAGNY TYAQLPPTKW
60 70 80 90 100
GARGTFKSAF NVSNITGPHT NTIIEWSNLL NSNGWVIYQK PANTTKLFKH
110 120 130 140 150
GPETYNSNLA AFELWYGKAG TSVTSDYYSS LQNNEKTVTA TSDSLILFWN
160 170 180 190 200
EGSTVLANKK VNFSWDMGGM LIKPTRGNRV DICMANMNDF NSSIFNWEEW
210 220 230 240 250
KHEFPRSDVN INVNMYTDYY LASEDPYTEL KALQQPNITT FEMKMMKIIR
260 270 280 290 300
NGSINLNEVV SKDSLWQEVR YARDITLECK IESEVVKGGG WGYDYTSVAF
310 320 330 340 350
KTVNHTYTYT RAGEIVNAHV TISFNNMKER SYGGSLPTDF KIGRFDVIDT
360 370 380 390 400
DTYMYIDYWD DSEIFKNMVY VRDLSANIGG FFYYAEMSYY FQIPVGAHPG
410 420 430 440 450
LHSSGVRFVY ERCLLSQQFT DQVALNSMRF IFRVTESNGW FMTSGNINTR
460 470 480 490 500
RIASGTGFAY ADGHTSQTVG NITFISLIPS NPNYQTPIAS SSTVRMDLER
510 520 530 540 550
KINDLRNDFN QLANSVALGD ILSLATSPLT FANLLESVPA IASSVKDVAA
560 570 580 590 600
NVMKKFRNTK MFKKATKAKY SEFIIGDLLE DVTNVARNSN GMNFDDITSA
610 620 630 640 650
VMVSTTNKLQ LTDVDTLSEI VARSADNFIP NRSYRMIEDG IVYEATPKRT
660 670 680 690 700
FSYDLTTLQQ REFDIDKFMR LASKSPVISA IVDFATLKAM RETYGVGTDV
710 720 730
IYKLVASDAP TILSFIDNNN PLIKSRIEEL LRQCRL
Length:736
Mass (Da):83,232
Last modified:May 1, 1992 - v1
Checksum:i356F9226D5016577
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74218 Genomic DNA. Translation: AAB00802.1.
PIRiD40822. VPXRPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74218 Genomic DNA. Translation: AAB00802.1.
PIRiD40822. VPXRPC.

3D structure databases

ProteinModelPortaliP26193.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVP4_ROTPC
AccessioniPrimary (citable) accession number: P26193
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.