Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P26193 (VP4_ROTPC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin

Cleaved into the following 2 chains:

  1. Outer capsid protein VP8*
  2. Outer capsid protein VP5*
OrganismRotavirus C (strain Pig/United States/Cowden/1980) (RV-C) [Complete proteome]
Taxonomic identifier10916 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors By similarity.

Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm By similarity.

VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies By similarity.

Subunit structure

VP4 is a homotrimer Potential.

Subcellular location

Outer capsid protein VP4: Virion. Host rough endoplasmic reticulum Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Outer capsid protein VP8*: Virion By similarity. Note: Outer capsid protein By similarity.

Outer capsid protein VP5*: Virion By similarity. Note: Outer capsid protein By similarity.

Post-translational modification

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion By similarity.

Sequence similarities

Belongs to the rotavirus VP4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 736736Outer capsid protein VP4
PRO_0000041099
Chain1 – 231231Outer capsid protein VP8* Potential
PRO_0000041100
Chain247 – 736490Outer capsid protein VP5* Potential
PRO_0000041101

Regions

Region393 – 41321Hydrophobic; possible role in virus entry into host cell Potential
Coiled coil488 – 52235 Potential

Sites

Site231 – 2322Cleavage Potential
Site247 – 2482Cleavage Potential

Amino acid modifications

Glycosylation391N-linked (GlcNAc...); by host Potential
Glycosylation611N-linked (GlcNAc...); by host Potential
Glycosylation641N-linked (GlcNAc...); by host Potential
Glycosylation931N-linked (GlcNAc...); by host Potential
Glycosylation1621N-linked (GlcNAc...); by host Potential
Glycosylation1911N-linked (GlcNAc...); by host Potential
Glycosylation2371N-linked (GlcNAc...); by host Potential
Glycosylation2511N-linked (GlcNAc...); by host Potential
Glycosylation3041N-linked (GlcNAc...); by host Potential
Glycosylation4711N-linked (GlcNAc...); by host Potential
Glycosylation6311N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P26193 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 356F9226D5016577

FASTA73683,232
        10         20         30         40         50         60 
MASSLYQQLI SQNYYSIGNE ILTDQQTTET VVDYVDAGNY TYAQLPPTKW GARGTFKSAF 

        70         80         90        100        110        120 
NVSNITGPHT NTIIEWSNLL NSNGWVIYQK PANTTKLFKH GPETYNSNLA AFELWYGKAG 

       130        140        150        160        170        180 
TSVTSDYYSS LQNNEKTVTA TSDSLILFWN EGSTVLANKK VNFSWDMGGM LIKPTRGNRV 

       190        200        210        220        230        240 
DICMANMNDF NSSIFNWEEW KHEFPRSDVN INVNMYTDYY LASEDPYTEL KALQQPNITT 

       250        260        270        280        290        300 
FEMKMMKIIR NGSINLNEVV SKDSLWQEVR YARDITLECK IESEVVKGGG WGYDYTSVAF 

       310        320        330        340        350        360 
KTVNHTYTYT RAGEIVNAHV TISFNNMKER SYGGSLPTDF KIGRFDVIDT DTYMYIDYWD 

       370        380        390        400        410        420 
DSEIFKNMVY VRDLSANIGG FFYYAEMSYY FQIPVGAHPG LHSSGVRFVY ERCLLSQQFT 

       430        440        450        460        470        480 
DQVALNSMRF IFRVTESNGW FMTSGNINTR RIASGTGFAY ADGHTSQTVG NITFISLIPS 

       490        500        510        520        530        540 
NPNYQTPIAS SSTVRMDLER KINDLRNDFN QLANSVALGD ILSLATSPLT FANLLESVPA 

       550        560        570        580        590        600 
IASSVKDVAA NVMKKFRNTK MFKKATKAKY SEFIIGDLLE DVTNVARNSN GMNFDDITSA 

       610        620        630        640        650        660 
VMVSTTNKLQ LTDVDTLSEI VARSADNFIP NRSYRMIEDG IVYEATPKRT FSYDLTTLQQ 

       670        680        690        700        710        720 
REFDIDKFMR LASKSPVISA IVDFATLKAM RETYGVGTDV IYKLVASDAP TILSFIDNNN 

       730 
PLIKSRIEEL LRQCRL 

« Hide

References

[1]"Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74218 Genomic DNA. Translation: AAB00802.1.
PIRVPXRPC. D40822.

3D structure databases

ProteinModelPortalP26193.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamPF00426. VP4_haemagglut. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP4_ROTPC
AccessionPrimary (citable) accession number: P26193
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families