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P26192

- VP3_ROTPC

UniProt

P26192 - VP3_ROTPC

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008175: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Protein VP3PRO_0000149536Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 (Potential). Interacts with VP2.Curated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26192-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVLGLFERG NNLNFADTYI YTWNKQYSYH ENAFLISNQV ATTIIIYLSD
60 70 80 90 100
TIVNEVDKAF TLLNSNGIPA LVIRKDHIGI FTSSNFTYDW QHKIVYFHEY
110 120 130 140 150
TYYKNNEFIV SDEFWLHTNI HELLPYKLLY YERGMRKLYD GEEYTLYNTA
160 170 180 190 200
TDDDILYKYI YEKDAIMSGD DYSELYDDKN FRNFVHFMRL LRMRFAVPFD
210 220 230 240 250
QLSNRVTRSR AFFKSKIHIG LRNESIPQAL DNINSQWINY SANGIMISEL
260 270 280 290 300
KGSGSYSEKR ISEFDIGQFK NYMNFLTLMF YIKNMKKRPS CTIIGAAPGY
310 320 330 340 350
WIPSMKRYFT IITYDDKIVD STEHHNRYFS EEDITKVRTN GVYIDVRSDF
360 370 380 390 400
DKSDWKKRRQ LVEEETKRWL EISYRLLEGK YVEAVLLKMT AMDIEIPDGY
410 420 430 440 450
FVHFPTTYRK SEYYLLIDKQ IIKKQKVKVT KSLMYNAINT IYSDNVFISG
460 470 480 490 500
KYTLRGKTEG VVALYCLSNT INQKDKVIQY ANSFSGTCMT VRLNNTYEVN
510 520 530 540 550
KVINLNNADY TFLPSDFVCP VNTVLTSYRG YAGVFGYAIT KDLKSDGNNH
560 570 580 590 600
IYIIPNARDE NNFDTFASHL GLSRYSHSKR FSESATTMSG YLFRDMVSGK
610 620 630 640 650
EDMGDTDKAN YASGHVFNAI AHYRFDYTYD IVGWLRLHKN KQFRVKSDIY
660 670 680 690
EEHTSDEVRN AIEAAYTYYL LDGDRVGKEY AKKIMEIWEA QV
Length:692
Mass (Da):81,378
Last modified:May 1, 1992 - v1
Checksum:iD679352CF22FE7DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74219 Genomic DNA. Translation: AAA99239.1.
PIRiC40822. P3XRPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74219 Genomic DNA. Translation: AAA99239.1 .
PIRi C40822. P3XRPC.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
    Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
    Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiVP3_ROTPC
AccessioniPrimary (citable) accession number: P26192
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 1, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3