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P26192

- VP3_ROTPC

UniProt

P26192 - VP3_ROTPC

Protein

Protein VP3

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.By similarity

    Catalytic activityi

    GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
    S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
    3. mRNA guanylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: InterPro

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein VP3
    Including the following 2 domains:
    mRNA guanylyltransferase (EC:2.7.7.50)
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
    Taxonomic identifieri10916 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008175: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.Curated

    GO - Cellular componenti

    1. viral nucleocapsid Source: InterPro

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692Protein VP3PRO_0000149536Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP1 Potential. Interacts with VP2.Curated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP3 family.Curated

    Family and domain databases

    InterProiIPR011181. VP3_Rotav.
    [Graphical view]
    PfamiPF06929. Rotavirus_VP3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26192-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVLGLFERG NNLNFADTYI YTWNKQYSYH ENAFLISNQV ATTIIIYLSD    50
    TIVNEVDKAF TLLNSNGIPA LVIRKDHIGI FTSSNFTYDW QHKIVYFHEY 100
    TYYKNNEFIV SDEFWLHTNI HELLPYKLLY YERGMRKLYD GEEYTLYNTA 150
    TDDDILYKYI YEKDAIMSGD DYSELYDDKN FRNFVHFMRL LRMRFAVPFD 200
    QLSNRVTRSR AFFKSKIHIG LRNESIPQAL DNINSQWINY SANGIMISEL 250
    KGSGSYSEKR ISEFDIGQFK NYMNFLTLMF YIKNMKKRPS CTIIGAAPGY 300
    WIPSMKRYFT IITYDDKIVD STEHHNRYFS EEDITKVRTN GVYIDVRSDF 350
    DKSDWKKRRQ LVEEETKRWL EISYRLLEGK YVEAVLLKMT AMDIEIPDGY 400
    FVHFPTTYRK SEYYLLIDKQ IIKKQKVKVT KSLMYNAINT IYSDNVFISG 450
    KYTLRGKTEG VVALYCLSNT INQKDKVIQY ANSFSGTCMT VRLNNTYEVN 500
    KVINLNNADY TFLPSDFVCP VNTVLTSYRG YAGVFGYAIT KDLKSDGNNH 550
    IYIIPNARDE NNFDTFASHL GLSRYSHSKR FSESATTMSG YLFRDMVSGK 600
    EDMGDTDKAN YASGHVFNAI AHYRFDYTYD IVGWLRLHKN KQFRVKSDIY 650
    EEHTSDEVRN AIEAAYTYYL LDGDRVGKEY AKKIMEIWEA QV 692
    Length:692
    Mass (Da):81,378
    Last modified:May 1, 1992 - v1
    Checksum:iD679352CF22FE7DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74219 Genomic DNA. Translation: AAA99239.1.
    PIRiC40822. P3XRPC.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74219 Genomic DNA. Translation: AAA99239.1 .
    PIRi C40822. P3XRPC.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011181. VP3_Rotav.
    [Graphical view ]
    Pfami PF06929. Rotavirus_VP3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
    PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
      Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
      Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiVP3_ROTPC
    AccessioniPrimary (citable) accession number: P26192
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3