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P26190

- RDRP_ROTPC

UniProt

P26190 - RDRP_ROTPC

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription (By similarity).PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Cofactori

Mg2+Curated

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008175: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10821082RNA-directed RNA polymerasePRO_0000149527Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 (Potential). Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak (By similarity).By similarityCurated

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini498 – 670173RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26190-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MDYDTLASKY LKFVYDFEDV TYQNNYFVTD EYKSDLEQYL KSIHDGEKIS
60 70 80 90 100
QSKIDSMETA LLTKVPKEKR CLISKLVFAY GKHGNVENKL LKYGTKDALT
110 120 130 140 150
HAIQKDVKPY ENNIITSEIF KDESEYTDTY MDPAINTSCQ SNCQAMMFTI
160 170 180 190 200
SEMKLTDIKN ATRLEKLFTI VAATINKYGM PRHNIRYRYE WETMKDKPYH
210 220 230 240 250
LAAWINSSIE MIACVVDHHT YMIARELIVK SFTNRTSLAK LVSSPMTVLT
260 270 280 290 300
AMLPIRGTVI TTENLELEYS SKSVNYLISE EMAEDFMKAI EQLRDEGLEI
310 320 330 340 350
YQDYYEKWFK SPDPLTFPNI ALIYSFSFHV GYRKQALSDA VYDQITVTYD
360 370 380 390 400
DNVNMEMYKE YSERIENEIF TILKDKVVHE DKRLEEYELS ALLSMSSASN
410 420 430 440 450
GVLREIDFGG QKVRSTKKNM HVIDDMYHKR YTTDIPPVDE RNPIPLGRRD
460 470 480 490 500
VPGRRTRAIF ILPYQYFIAQ HSFAEMMLKY AKREREYSEF YSQANQVLSY
510 520 530 540 550
GDVTRYLDSN SILCFTDVSQ WDASQHNTRV LRRSIIRAME RLKQLTHNTN
560 570 580 590 600
IHKAIDIYIQ SQKNLENSYV LIDKKAIQYG ATASGEKQTK IMNSIANKAL
610 620 630 640 650
IQTVLGKLMT DYTFDVKMIR VDGDDNYAIV RFPTAITEKL LSEFTSKLRS
660 670 680 690 700
YYSEMNVKVK ALASLTGCEI AKRYIAGGML FFRAGVNILN HEKRNQDSAY
710 720 730 740 750
DMAATLYANY IVNALRGLTM SRTFILVKIC QMTSIKITGT LRLFPMKSIL
760 770 780 790 800
ALNSTFKVFD EVDYVINYPI SELFIQLQRK LSSIKAKSKI ADNIAKSPQF
810 820 830 840 850
KSYVEFLNKS LTADENPIVS DGIKLTEKAK LNSYAPIALE KRRDQFNMMV
860 870 880 890 900
SFLQNPTTFK SETVVTINDV LYFISGFIKI NSSVALPKEE NNTMPLLPVT
910 920 930 940 950
IKRTLNYFGL RTHDYDIKGS SSTMSKIIKQ YSVYTPGIEE LYEVVNKSID
960 970 980 990 1000
SIRGYFASFN VPKADVDTYI STQMYKHDRF KILQAYIYNL LSVNYGMYQL
1010 1020 1030 1040 1050
VDLNSAKFFD HVIHTPMAKT PTAVFMIDLA LRLKVINHCI EKGEVITVSV
1060 1070 1080
HANKTDYLKL WRMLWNVKTM NSPYSKNSMF DE
Length:1,082
Mass (Da):125,082
Last modified:May 1, 1992 - v1
Checksum:i4FE5D43379E89D96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74216 Genomic DNA. Translation: AAB00801.1.
PIRiA40822. P1XRPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74216 Genomic DNA. Translation: AAB00801.1 .
PIRi A40822. P1XRPC.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
    Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
    Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRDRP_ROTPC
AccessioniPrimary (citable) accession number: P26190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3