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P26190

- RDRP_ROTPC

UniProt

P26190 - RDRP_ROTPC

Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Cofactori

    Magnesium.Curated

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Magnesium, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein VP1
    OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
    Taxonomic identifieri10916 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000008175: Genome

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10821082RNA-directed RNA polymerasePRO_0000149527Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP3 Potential. Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak By similarity.By similarityCurated

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini498 – 670173RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view]
    PfamiPF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26190-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYDTLASKY LKFVYDFEDV TYQNNYFVTD EYKSDLEQYL KSIHDGEKIS     50
    QSKIDSMETA LLTKVPKEKR CLISKLVFAY GKHGNVENKL LKYGTKDALT 100
    HAIQKDVKPY ENNIITSEIF KDESEYTDTY MDPAINTSCQ SNCQAMMFTI 150
    SEMKLTDIKN ATRLEKLFTI VAATINKYGM PRHNIRYRYE WETMKDKPYH 200
    LAAWINSSIE MIACVVDHHT YMIARELIVK SFTNRTSLAK LVSSPMTVLT 250
    AMLPIRGTVI TTENLELEYS SKSVNYLISE EMAEDFMKAI EQLRDEGLEI 300
    YQDYYEKWFK SPDPLTFPNI ALIYSFSFHV GYRKQALSDA VYDQITVTYD 350
    DNVNMEMYKE YSERIENEIF TILKDKVVHE DKRLEEYELS ALLSMSSASN 400
    GVLREIDFGG QKVRSTKKNM HVIDDMYHKR YTTDIPPVDE RNPIPLGRRD 450
    VPGRRTRAIF ILPYQYFIAQ HSFAEMMLKY AKREREYSEF YSQANQVLSY 500
    GDVTRYLDSN SILCFTDVSQ WDASQHNTRV LRRSIIRAME RLKQLTHNTN 550
    IHKAIDIYIQ SQKNLENSYV LIDKKAIQYG ATASGEKQTK IMNSIANKAL 600
    IQTVLGKLMT DYTFDVKMIR VDGDDNYAIV RFPTAITEKL LSEFTSKLRS 650
    YYSEMNVKVK ALASLTGCEI AKRYIAGGML FFRAGVNILN HEKRNQDSAY 700
    DMAATLYANY IVNALRGLTM SRTFILVKIC QMTSIKITGT LRLFPMKSIL 750
    ALNSTFKVFD EVDYVINYPI SELFIQLQRK LSSIKAKSKI ADNIAKSPQF 800
    KSYVEFLNKS LTADENPIVS DGIKLTEKAK LNSYAPIALE KRRDQFNMMV 850
    SFLQNPTTFK SETVVTINDV LYFISGFIKI NSSVALPKEE NNTMPLLPVT 900
    IKRTLNYFGL RTHDYDIKGS SSTMSKIIKQ YSVYTPGIEE LYEVVNKSID 950
    SIRGYFASFN VPKADVDTYI STQMYKHDRF KILQAYIYNL LSVNYGMYQL 1000
    VDLNSAKFFD HVIHTPMAKT PTAVFMIDLA LRLKVINHCI EKGEVITVSV 1050
    HANKTDYLKL WRMLWNVKTM NSPYSKNSMF DE 1082
    Length:1,082
    Mass (Da):125,082
    Last modified:May 1, 1992 - v1
    Checksum:i4FE5D43379E89D96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74216 Genomic DNA. Translation: AAB00801.1.
    PIRiA40822. P1XRPC.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74216 Genomic DNA. Translation: AAB00801.1 .
    PIRi A40822. P1XRPC.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view ]
    Pfami PF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
      Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
      Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRDRP_ROTPC
    AccessioniPrimary (citable) accession number: P26190
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3