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P26190

- RDRP_ROTPC

UniProt

P26190 - RDRP_ROTPC

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium Reviewed prediction.

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Magnesium, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus C (strain Pig/United States/Cowden/1980) (RV-C)
Taxonomic identifieri10916 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000008175: Genome

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10821082RNA-directed RNA polymerasePRO_0000149527Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 Reviewed prediction. Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini498 – 670173RdRp catalyticAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26190-1 [UniParc]FASTAAdd to Basket

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MDYDTLASKY LKFVYDFEDV TYQNNYFVTD EYKSDLEQYL KSIHDGEKIS     50
QSKIDSMETA LLTKVPKEKR CLISKLVFAY GKHGNVENKL LKYGTKDALT 100
HAIQKDVKPY ENNIITSEIF KDESEYTDTY MDPAINTSCQ SNCQAMMFTI 150
SEMKLTDIKN ATRLEKLFTI VAATINKYGM PRHNIRYRYE WETMKDKPYH 200
LAAWINSSIE MIACVVDHHT YMIARELIVK SFTNRTSLAK LVSSPMTVLT 250
AMLPIRGTVI TTENLELEYS SKSVNYLISE EMAEDFMKAI EQLRDEGLEI 300
YQDYYEKWFK SPDPLTFPNI ALIYSFSFHV GYRKQALSDA VYDQITVTYD 350
DNVNMEMYKE YSERIENEIF TILKDKVVHE DKRLEEYELS ALLSMSSASN 400
GVLREIDFGG QKVRSTKKNM HVIDDMYHKR YTTDIPPVDE RNPIPLGRRD 450
VPGRRTRAIF ILPYQYFIAQ HSFAEMMLKY AKREREYSEF YSQANQVLSY 500
GDVTRYLDSN SILCFTDVSQ WDASQHNTRV LRRSIIRAME RLKQLTHNTN 550
IHKAIDIYIQ SQKNLENSYV LIDKKAIQYG ATASGEKQTK IMNSIANKAL 600
IQTVLGKLMT DYTFDVKMIR VDGDDNYAIV RFPTAITEKL LSEFTSKLRS 650
YYSEMNVKVK ALASLTGCEI AKRYIAGGML FFRAGVNILN HEKRNQDSAY 700
DMAATLYANY IVNALRGLTM SRTFILVKIC QMTSIKITGT LRLFPMKSIL 750
ALNSTFKVFD EVDYVINYPI SELFIQLQRK LSSIKAKSKI ADNIAKSPQF 800
KSYVEFLNKS LTADENPIVS DGIKLTEKAK LNSYAPIALE KRRDQFNMMV 850
SFLQNPTTFK SETVVTINDV LYFISGFIKI NSSVALPKEE NNTMPLLPVT 900
IKRTLNYFGL RTHDYDIKGS SSTMSKIIKQ YSVYTPGIEE LYEVVNKSID 950
SIRGYFASFN VPKADVDTYI STQMYKHDRF KILQAYIYNL LSVNYGMYQL 1000
VDLNSAKFFD HVIHTPMAKT PTAVFMIDLA LRLKVINHCI EKGEVITVSV 1050
HANKTDYLKL WRMLWNVKTM NSPYSKNSMF DE 1082
Length:1,082
Mass (Da):125,082
Last modified:May 1, 1992 - v1
Checksum:i4FE5D43379E89D96
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74216 Genomic DNA. Translation: AAB00801.1.
PIRiA40822. P1XRPC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74216 Genomic DNA. Translation: AAB00801.1 .
PIRi A40822. P1XRPC.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
IPR022071. Rotavirus_VP1.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
PF12289. Rotavirus_VP1. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of the four larger proteins of a porcine group C rotavirus and comparison with the equivalent group A rotavirus proteins."
    Bremont M., Juste-Lesage P., Chabanne-Vautherot D., Charpilienne A., Cohen J.
    Virology 186:684-692(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRDRP_ROTPC
AccessioniPrimary (citable) accession number: P26190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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