Regulatory protein ada - Salmonella typhimurium

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Reviewed, UniProtKB/Swiss-Prot P26189 (ADA_SALTY)

Last modified June 16, 2009. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Regulatory protein ada
Alternative name(s):
    Regulatory protein of adaptative response
Cleaved into the following chain:
    1- Recommended name:
            Methylated-DNA--protein-cysteine methyltransferase
              EC=2.1.1.63
        Alternative name(s):
            O-6-methylguanine-DNA alkyltransferase

Gene names

Name:	ada
Ordered Locus Names:	STM2265

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

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Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine. The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	In the C-terminal section; belongs to the MGMT family. Contains 1 HTH araC/xylS-type DNA-binding domain.

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Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 353

353

Regulatory protein ada

PRO_0000018749

Chain

179 – 353

175

Methylated-DNA--protein-cysteine methyltransferase

PRO_0000018750

Regions

DNA binding

102 – 121

H-T-H motif By similarity

Sites

Active site

Nucleophile; methyl group acceptor from phosphotriester By similarity

Active site

321

Nucleophile; methyl group acceptor By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Binding site

DNA By similarity

Binding site

DNA By similarity

Binding site

DNA By similarity

Site

128 – 129

Cleavage By similarity

Site

178 – 179

Cleavage By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P26189-1 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: ACE9E13C7EF6C114
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FASTA

353

39,348

        10         20         30         40         50         60 
MMKKALLIDD ECWLRVQARD ASADGRFVFA VRTTGVFCRP SCRSKRALRK NVRFFANAQQ 

        70         80         90        100        110        120 
ALDAGFRPCK RCQPDNARAQ QRRLDKIACA CRLLEQETPV TLAFLAQAVA MSPFHLHRLF 

       130        140        150        160        170        180 
KASTGMTPKG WQQAWRARRL REALAKGEPI TAAIYRAGFP DSSSYYRHAD QTLGMTAKQF 

       190        200        210        220        230        240 
RKGGDNVSVR YALTDWVYGR CLVAESERGI CAILPGDSDD ALLAELHTLF PSARHEPADA 

       250        260        270        280        290        300 
LFQQRVRQVV AAINTRDVLL SLPLDIQGTA FQQQVWQALC AIPCGETVSY QQLAATIGKP 

       310        320        330        340        350 
TAVRAVASAC GANKLAMVIP CHRVVRRDGA LSGYRWGVRR KAQLLKREAQ KEE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase." Hakura A., Morimoto K., Sofuni T., Nohmi T. J. Bacteriol. 173:3663-3672(1991) [PubMed: 1904855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

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Cross-references

Sequence databases
	D90221 Genomic DNA. Translation: BAA14252.1. Different initiation. AE008801 Genomic DNA. Translation: AAL21167.1.
PIR	XYEBOT. A39433.
RefSeq	NP_461208.1.
3D structure databases
HSSP	HSSP built from PDB template 1SFE based on UniProtKB P06134.
SMR	P26189. Positions 187-350.
ModBase	Search...
Protein family/group databases
AraC-XylS	Search...
Genome annotation databases
GeneID	1253787.
GenomeReviews	Gene locus STM2265 in contig AE006468_GR.
KEGG	stm:STM2265.
NMPDR	fig\|99287.1.peg.2189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P26189.
OMA	P26189. SNSRFYE.
Enzyme and pathway databases
BioCyc	STYP99287:STM2265-MON.
BRENDA	2.1.1.63. 2.
Family and domain databases
InterPro	IPR004026. Ada_DNA_repair_Zn-bd. IPR016221. Bifunct_regulatory_prot_Ada. IPR000005. HTH_AraC-typ. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_domain. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA_bd. IPR008332. MethylG_MeTrfase. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:3.40.10.10. Ada_DNA_repair_Zn-bd. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
Pfam	PF02805. Ada_Zn_binding. 1 hit. PF01035. DNA_binding_1. 1 hit. PF00165. HTH_AraC. 2 hits. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
PIRSF	PIRSF000409. Ada. 1 hit.
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 2 hits. PS01124. HTH_ARAC_FAMILY_2. 1 hit. PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ADA_SALTY

Accession

Primary (citable) accession number: P26189

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2002
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents