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P26189 (ADA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Regulatory protein ada
Alternative name(s):
Regulatory protein of adaptative response

Cleaved into the following chain:

  1. Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
    Alternative name(s):
    O-6-methylguanine-DNA alkyltransferase
Gene names
Name:ada
Ordered Locus Names:STM2265
OrganismSalmonella typhimurium
Taxonomic identifier90371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.

The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB.

Catalytic activity

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.

Sequence similarities

In the C-terminal section; belongs to the MGMT family.

Contains 1 HTH araC/xylS-type DNA-binding domain.

Sequence caution

The sequence BAA14252.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Regulatory protein ada
PRO_0000018749
Chain179 – 353175Methylated-DNA--protein-cysteine methyltransferase
PRO_0000018750

Regions

DNA binding102 – 12120H-T-H motif By similarity

Sites

Active site381Nucleophile; methyl group acceptor from phosphotriester By similarity
Active site3211Nucleophile; methyl group acceptor By similarity
Metal binding381Zinc By similarity
Metal binding421Zinc By similarity
Metal binding691Zinc By similarity
Metal binding721Zinc By similarity
Binding site341DNA By similarity
Binding site431DNA By similarity
Binding site671DNA By similarity
Site128 – 1292Cleavage By similarity
Site178 – 1792Cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
P26189 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: ACE9E13C7EF6C114

FASTA35339,348
        10         20         30         40         50         60 
MMKKALLIDD ECWLRVQARD ASADGRFVFA VRTTGVFCRP SCRSKRALRK NVRFFANAQQ 

        70         80         90        100        110        120 
ALDAGFRPCK RCQPDNARAQ QRRLDKIACA CRLLEQETPV TLAFLAQAVA MSPFHLHRLF 

       130        140        150        160        170        180 
KASTGMTPKG WQQAWRARRL REALAKGEPI TAAIYRAGFP DSSSYYRHAD QTLGMTAKQF 

       190        200        210        220        230        240 
RKGGDNVSVR YALTDWVYGR CLVAESERGI CAILPGDSDD ALLAELHTLF PSARHEPADA 

       250        260        270        280        290        300 
LFQQRVRQVV AAINTRDVLL SLPLDIQGTA FQQQVWQALC AIPCGETVSY QQLAATIGKP 

       310        320        330        340        350 
TAVRAVASAC GANKLAMVIP CHRVVRRDGA LSGYRWGVRR KAQLLKREAQ KEE

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase."
Hakura A., Morimoto K., Sofuni T., Nohmi T.
J. Bacteriol. 173:3663-3672(1991) [PubMed: 1904855] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed: 11677609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D90221 Genomic DNA. Translation: BAA14252.1. Different initiation.
AE006468 Genomic DNA. Translation: AAL21167.1.
PIRXYEBOT. A39433.
RefSeqNP_461208.1. NC_003197.1.

3D structure databases

ProteinModelPortalP26189.
SMRP26189. Positions 1-146, 187-350.
ModBaseSearch...

Protein family/group databases

AraC-XylSSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1253787.
GenomeReviewsGene locus STM2265 in contig AE006468_GR.
KEGGstm:STM2265.
NMPDRfig|99287.1.peg.2189.
PATRIC32383139. VBISalEnt20916_2399.

Phylogenomic databases

HOGENOMHBG752398.
OMASNSRFYE.
ProtClustDBPRK15435.

Enzyme and pathway databases

BioCycSTYP99287:STM2265-MONOMER.

Family and domain databases

InterProIPR004026. Ada_DNA_repair_Zn-bd.
IPR016221. Bifunct_regulatory_prot_Ada.
IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR018062. HTH_AraC-typ_sub_2.
IPR018060. HTH_AraC_DNA-bd.
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]
Gene3DG3DSA:3.40.10.10. Ada_DNA_repair_Zn-bd. 1 hit.
G3DSA:1.10.10.60. Homeodomain-rel. 2 hits.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KOK10778.
PfamPF02805. Ada_Zn_binding. 1 hit.
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
PIRSFPIRSF000409. Ada. 1 hit.
SMARTSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMSSF57884. Ada_DNA_repair_Zn-bd. 1 hit.
SSF46689. Homeodomain_like. 1 hit.
SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMsTIGR00589. Ogt. 1 hit.
PROSITEPS00041. HTH_ARAC_FAMILY_1. 2 hits.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
PS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADA_SALTY
AccessionPrimary (citable) accession number: P26189
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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