P26188 (MGMT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	MGT1
Ordered Locus Names:	YDL200C
ORF Names:	D1204

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine. Prefers double-stranded DNA over single-stranded DNA as subtsrate. Ref.1 Ref.7 Ref.8
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Subcellular location	Nucleus.
Induction	In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents. Ref.9
Miscellaneous	Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells. Ref.7 This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.
Sequence similarities	Belongs to the MGMT family.
Sequence caution	The sequence AAA34780.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA42920.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA67469.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA98777.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA98778.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA dealkylation involved in DNA repair Inferred from mutant phenotype Ref.2. Source: SGD
Cellular component	nucleus Inferred by curator Ref.2. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from direct assay Ref.1Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139362
Sites
	Active site	151	1	Nucleophile; methyl group acceptor By similarity
	Binding site	120	1	DNA By similarity
	Binding site	121	1	DNA; via amide nitrogen By similarity
	Binding site	134	1	DNA By similarity
	Binding site	157	1	DNA; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26188 [UniParc]. Last modified May 20, 2008. Version 3. Checksum: 956E71B47016E47D	FASTA	188	21,499
	10 20 30 40 50 60 MKELLYYTFI ETEVTGAFLV FREKTQNLVF ASLGNDKLFL LGKVEGFLKK HEKQDTMYDL 70 80 90 100 110 120 QELKEAETYK KSIENYTICL ENKMPLPSGA IPFEFLFGTD FQRKVWNELL NVEHGHVVTY 130 140 150 160 170 180 GDIAKRIGKP TAARSVGRAC GSNNLALLVP CHRIVGSNRK LTGYKWSCKL KEQLLNNEKE NSLSLSRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene." Xiao W., Derfler B., Chen J., Samson L. EMBO J. 10:2179-2186(1991) [PubMed: 2065659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions." Xiao W., Samson L. Nucleic Acids Res. 20:3599-3606(1992) [PubMed: 1641326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: ATCC 44774 / DBY747.
[3]	"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA." Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R. Yeast 13:163-169(1997) [PubMed: 9046097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein." Verhasselt P., Voet M., Mathys J., Volckaert G. Yeast 12:1065-1070(1996) [PubMed: 8896272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135. Strain: ATCC 96604 / S288c / FY1679.
[7]	"Identification and preliminary characterization of an O6-methylguanine DNA repair methyltransferase in the yeast Saccharomyces cerevisiae." Sassanfar M., Samson L. J. Biol. Chem. 265:20-25(1990) [PubMed: 2403555] [Abstract] Cited for: FUNCTION, CHARACTERIZATION, LEVEL OF PROTEIN EXPRESSION.
[8]	"Relative efficiencies of the bacterial, yeast, and human DNA methyltransferases for the repair of O6-methylguanine and O4-methylthymine." Sassanfar M., Dosanjh M.K., Essigmann J.M., Samson L. J. Biol. Chem. 266:2767-2771(1991) [PubMed: 1993655] [Abstract] Cited for: FUNCTION.
[9]	"Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene." Joo J.H., Rho J.K., Kim J.H., Kim W.J., Choe S.Y., Park S.D. Cell. Mol. Biol. 41:545-553(1995) [PubMed: 7549791] [Abstract] Cited for: INDUCTION.
[10]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[11]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60368 Genomic DNA. Translation: CAA42920.1. Different initiation. M94227 Genomic DNA. Translation: AAA34780.1. Different initiation. X99000 Genomic DNA. Translation: CAA67469.1. Different initiation. Z74248 Genomic DNA. Translation: CAA98778.1. Different initiation. Z74247 Genomic DNA. Translation: CAA98777.1. Different initiation. X83276 Genomic DNA. Translation: CAA58247.1. BK006938 Genomic DNA. Translation: DAA11664.1.
PIR	XUBYMC. S29370.
RefSeq	NP_010081.2. NM_001180260.1.
3D structure databases
ProteinModelPortal	P26188.
SMR	P26188. Positions 5-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7461N.
IntAct	P26188. 18 interactions.
MINT	MINT-674377.
STRING	P26188.
Proteomic databases
PeptideAtlas	P26188.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL200C; YDL200C; YDL200C.
GeneID	851327.
KEGG	sce:YDL200C.
NMPDR	fig|4932.3.peg.814.
Organism-specific databases
SGD	S000002359. MGT1.
Phylogenomic databases
eggNOG	fuNOG11947.
HOGENOM	HBG752398.
OMA	YTICLEN.
OrthoDB	EOG48H0CX.
Gene expression databases
ArrayExpress	P26188.
Genevestigator	P26188.
GermOnline	YDL200C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR008332. MethylG_MeTrfase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K00567.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. Ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968381.

Entry information

Entry name

MGMT_YEAST

Accession

Primary (citable) accession number: P26188
Secondary accession number(s): D6VRF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program