Reviewed, UniProtKB/Swiss-Prot P26188 (MGMT_YEAST)

Names and origin

Protein names

Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
      Short name=MGMT
    DNA repair MTase
    O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	MGT1
Ordered Locus Names:	YDL200C
ORF Names:	D1204

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine. Prefers double-stranded DNA over single-stranded DNA as subtsrate. Ref.1 Ref.6 Ref.7
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Subcellular location	Nucleus.
Induction	In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents. Ref.8
Miscellaneous	Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells. Ref.6
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA dealkylation Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Ref.2 Inferred by curator. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW methylated-DNA-[protein]-cysteine S-methyltransferase activity Ref.1 Ref.6 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139362
Sites
	Active site	151	1	Nucleophile; methyl group acceptor By similarity
	Binding site	120	1	DNA By similarity
	Binding site	121	1	DNA; via amide nitrogen By similarity
	Binding site	134	1	DNA By similarity
	Binding site	157	1	DNA; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26188-1 [UniParc]. Last modified May 20, 2008. Version 3. Checksum: 956E71B47016E47D	FASTA	188	21,499
	10 20 30 40 50 60 MKELLYYTFI ETEVTGAFLV FREKTQNLVF ASLGNDKLFL LGKVEGFLKK HEKQDTMYDL 70 80 90 100 110 120 QELKEAETYK KSIENYTICL ENKMPLPSGA IPFEFLFGTD FQRKVWNELL NVEHGHVVTY 130 140 150 160 170 180 GDIAKRIGKP TAARSVGRAC GSNNLALLVP CHRIVGSNRK LTGYKWSCKL KEQLLNNEKE NSLSLSRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene." Xiao W., Derfler B., Chen J., Samson L. EMBO J. 10:2179-2186(1991) [PubMed: 2065659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions." Xiao W., Samson L. Nucleic Acids Res. 20:3599-3606(1992) [PubMed: 1641326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION. Strain: ATCC 44774 / DBY747.
[3]	"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA." Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R. Yeast 13:163-169(1997) [PubMed: 9046097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein." Verhasselt P., Voet M., Mathys J., Volckaert G. Yeast 12:1065-1070(1996) [PubMed: 8896272] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Identification and preliminary characterization of an O6-methylguanine DNA repair methyltransferase in the yeast Saccharomyces cerevisiae." Sassanfar M., Samson L. J. Biol. Chem. 265:20-25(1990) [PubMed: 2403555] [Abstract] Cited for: FUNCTION, CHARACTERIZATION, LEVEL OF PROTEIN EXPRESSION.
[7]	"Relative efficiencies of the bacterial, yeast, and human DNA methyltransferases for the repair of O6-methylguanine and O4-methylthymine." Sassanfar M., Dosanjh M.K., Essigmann J.M., Samson L. J. Biol. Chem. 266:2767-2771(1991) [PubMed: 1993655] [Abstract] Cited for: FUNCTION.
[8]	"Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene." Joo J.H., Rho J.K., Kim J.H., Kim W.J., Choe S.Y., Park S.D. Cell. Mol. Biol. 41:545-553(1995) [PubMed: 7549791] [Abstract] Cited for: INDUCTION.
[9]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[10]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60368 Genomic DNA. Translation: CAA42920.1. Different initiation. M94227 Genomic DNA. Translation: AAA34780.1. Different initiation. X99000 Genomic DNA. Translation: CAA67469.1. Different initiation. Z74248 Genomic DNA. Translation: CAA98778.1. Different initiation. Z74247 Genomic DNA. Translation: CAA98777.1. Different initiation. X83276 Genomic DNA. Translation: CAA58247.1.
PIR	XUBYMC. S29370.
RefSeq	NP_010081.2.
3D structure databases
HSSP	HSSP built from PDB template 1QNT based on UniProtKB P16455.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7461N.
Proteomic databases
PeptideAtlas	P26188.
PRIDE	P26188.
Genome annotation databases
Ensembl	YDL200C. Saccharomyces cerevisiae. [Contig view]
GeneID	851327.
GenomeReviews	Gene locus YDL200C in contig Z71256_GR.
KEGG	sce:YDL200C.
NMPDR	fig|4932.3.peg.814.
Organism-specific databases
CYGD	YDL200c.
SGD	S000002359. MGT1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P26188.
Enzyme and pathway databases
BRENDA	2.1.1.63. 250.
Gene expression databases
GermOnline	YDL200C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA_bd. IPR008332. MethylG_MeTrfase. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968381.

Entry information

Entry name

MGMT_YEAST

Accession

Primary (citable) accession number: P26188

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)