Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

MGT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Also repairs O-4-methylthymine. Prefers double-stranded DNA over single-stranded DNA as substrate.3 Publications

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201DNABy similarity
Binding sitei121 – 1211DNA; via amide nitrogenBy similarity
Binding sitei134 – 1341DNABy similarity
Active sitei151 – 1511Nucleophile; methyl group acceptorPROSITE-ProRule annotation
Binding sitei157 – 1571DNA; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

  • DNA dealkylation involved in DNA repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
DNA repair MTase
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:MGT1
Ordered Locus Names:YDL200C
ORF Names:D1204
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL200C.
SGDiS000002359. MGT1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Methylated-DNA--protein-cysteine methyltransferasePRO_0000139362Add
BLAST

Proteomic databases

PeptideAtlasiP26188.

Expressioni

Inductioni

In contrast to some bacterial and mammalian enzymes, MGT1 is not induced by alkylating agents.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31846. 34 interactions.
DIPiDIP-7461N.
MINTiMINT-674377.

Structurei

3D structure databases

ProteinModelPortaliP26188.
SMRiP26188. Positions 96-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000112520.
HOGENOMiHOG000244137.
InParanoidiP26188.
KOiK00567.
OMAiYTICLEN.
OrthoDBiEOG7BKD6H.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26188-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKELLYYTFI ETEVTGAFLV FREKTQNLVF ASLGNDKLFL LGKVEGFLKK
60 70 80 90 100
HEKQDTMYDL QELKEAETYK KSIENYTICL ENKMPLPSGA IPFEFLFGTD
110 120 130 140 150
FQRKVWNELL NVEHGHVVTY GDIAKRIGKP TAARSVGRAC GSNNLALLVP
160 170 180
CHRIVGSNRK LTGYKWSCKL KEQLLNNEKE NSLSLSRL
Length:188
Mass (Da):21,499
Last modified:May 20, 2008 - v3
Checksum:i956E71B47016E47D
GO

Sequence cautioni

The sequence AAA34780.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA42920.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA67469.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA98777.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA98778.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60368 Genomic DNA. Translation: CAA42920.1. Different initiation.
M94227 Genomic DNA. Translation: AAA34780.1. Different initiation.
X99000 Genomic DNA. Translation: CAA67469.1. Different initiation.
Z74248 Genomic DNA. Translation: CAA98778.1. Different initiation.
Z74247 Genomic DNA. Translation: CAA98777.1. Different initiation.
X83276 Genomic DNA. Translation: CAA58247.1.
BK006938 Genomic DNA. Translation: DAA11664.1.
PIRiS29370. XUBYMC.
RefSeqiNP_010081.2. NM_001180260.1.

Genome annotation databases

EnsemblFungiiYDL200C; YDL200C; YDL200C.
GeneIDi851327.
KEGGisce:YDL200C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60368 Genomic DNA. Translation: CAA42920.1. Different initiation.
M94227 Genomic DNA. Translation: AAA34780.1. Different initiation.
X99000 Genomic DNA. Translation: CAA67469.1. Different initiation.
Z74248 Genomic DNA. Translation: CAA98778.1. Different initiation.
Z74247 Genomic DNA. Translation: CAA98777.1. Different initiation.
X83276 Genomic DNA. Translation: CAA58247.1.
BK006938 Genomic DNA. Translation: DAA11664.1.
PIRiS29370. XUBYMC.
RefSeqiNP_010081.2. NM_001180260.1.

3D structure databases

ProteinModelPortaliP26188.
SMRiP26188. Positions 96-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31846. 34 interactions.
DIPiDIP-7461N.
MINTiMINT-674377.

Proteomic databases

PeptideAtlasiP26188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL200C; YDL200C; YDL200C.
GeneIDi851327.
KEGGisce:YDL200C.

Organism-specific databases

EuPathDBiFungiDB:YDL200C.
SGDiS000002359. MGT1.

Phylogenomic databases

GeneTreeiENSGT00730000112520.
HOGENOMiHOG000244137.
InParanoidiP26188.
KOiK00567.
OMAiYTICLEN.
OrthoDBiEOG7BKD6H.

Enzyme and pathway databases

BioCyciYEAST:G3O-29584-MONOMER.

Miscellaneous databases

NextBioi968381.
PROiP26188.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary sequence and biological functions of a Saccharomyces cerevisiae O6-methylguanine/O4-methylthymine DNA repair methyltransferase gene."
    Xiao W., Derfler B., Chen J., Samson L.
    EMBO J. 10:2179-2186(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions."
    Xiao W., Samson L.
    Nucleic Acids Res. 20:3599-3606(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
    Strain: ATCC 44774 / DBY747.
  3. "The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
    Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
    Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
    Verhasselt P., Voet M., Mathys J., Volckaert G.
    Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
    Strain: ATCC 96604 / S288c / FY1679.
  7. "Identification and preliminary characterization of an O6-methylguanine DNA repair methyltransferase in the yeast Saccharomyces cerevisiae."
    Sassanfar M., Samson L.
    J. Biol. Chem. 265:20-25(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION, LEVEL OF PROTEIN EXPRESSION.
  8. "Relative efficiencies of the bacterial, yeast, and human DNA methyltransferases for the repair of O6-methylguanine and O4-methylthymine."
    Sassanfar M., Dosanjh M.K., Essigmann J.M., Samson L.
    J. Biol. Chem. 266:2767-2771(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene."
    Joo J.H., Rho J.K., Kim J.H., Kim W.J., Choe S.Y., Park S.D.
    Cell. Mol. Biol. 41:545-553(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  11. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
    Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
    Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.

Entry informationi

Entry nameiMGMT_YEAST
AccessioniPrimary (citable) accession number: P26188
Secondary accession number(s): D6VRF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 20, 2008
Last modified: May 11, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 150 molecules/cell in log phase YPD medium, but not detectable in stationary phase cells.1 Publication
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.