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Reviewed, UniProtKB/Swiss-Prot P26187 (MGMT_MOUSE)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
      Short name=MGMT
    O-6-methylguanine-DNA-alkyltransferase
Gene names
Name: Mgmt
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activity

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the MGMT family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 211210Methylated-DNA--protein-cysteine methyltransferase
PRO_0000139360

Sites

Active site1491Nucleophile; methyl group acceptor By similarity
Metal binding51Zinc By similarity
Metal binding241Zinc By similarity
Metal binding291Zinc By similarity
Metal binding891Zinc By similarity
Binding site991DNA; via amide nitrogen By similarity
Binding site1181DNA By similarity
Binding site1191DNA; via amide nitrogen By similarity
Binding site1271DNA By similarity
Binding site1321DNA By similarity
Binding site1551DNA; via amide nitrogen By similarity

Amino acid modifications

Modified residue2051Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P26187-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 838BB92B9A1083D6

FASTA21122,435
        10         20         30         40         50         60 
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP EVLGGPEGVP 

        70         80         90        100        110        120 
EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGETVSYQQ 

       130        140        150        160        170        180 
LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP 

       190        200        210 
TGQPASKGLG LTGTWLKSSF ESTSSEPSGR N 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli."
Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S.
Biochemistry 31:1897-1903(1992) [PubMed: 1371399] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"MGMT sequence of B6 and C3H mice."
Shimada Y., Kakinuma S., Kubo A., Nishimura M.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H and C57BL/6.
Tissue: Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
IPIIPI00229662.
PIRA41809.
RefSeqNP_032624.1.
UniGeneMm.440219

3D structure databases

HSSPHSSP built from PDB template 1EH7 based on UniProtKB P16455.
SMRP26187. Positions 5-185.
ModBaseSearch...

PTM databases

PhosphoSiteP26187.

Proteomic databases

PRIDEP26187.

Genome annotation databases

EnsemblENSMUSG00000054612. Mus musculus. [Contig view]
GeneID17314.
KEGGmmu:17314.
NMPDRfig|10090.3.peg.17835.

Organism-specific databases

MGIMGI:96977. Mgmt.

Phylogenomic databases

HOGENOMP26187.
HOVERGENP26187.
OMAP26187. CENGVHT.

Enzyme and pathway databases

BRENDA2.1.1.63. 244.

Gene expression databases

ArrayExpressP26187.
BgeeP26187.
CleanExMM_MGMT.
GermOnlineENSMUSG00000054612. Mus musculus.

Family and domain databases

InterProIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA_bd.
IPR008332. MethylG_MeTrfase.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHERPTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
PfamPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00589. ogt. 1 hit.
PROSITEPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio291876.
SOURCESearch...

Entry information

Entry nameMGMT_MOUSE
AccessionPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 70 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents