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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi5ZincBy similarity1
Metal bindingi24ZincBy similarity1
Metal bindingi29ZincBy similarity1
Metal bindingi89ZincBy similarity1
Binding sitei99DNA; via amide nitrogenBy similarity1
Binding sitei118DNABy similarity1
Binding sitei119DNA; via amide nitrogenBy similarity1
Binding sitei127DNABy similarity1
Binding sitei132DNABy similarity1
Active sitei149Nucleophile; methyl group acceptorPROSITE-ProRule annotation1
Binding sitei155DNA; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96977. Mgmt.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393601 – 211Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei205PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP26187.
MaxQBiP26187.
PaxDbiP26187.
PeptideAtlasiP26187.
PRIDEiP26187.

PTM databases

iPTMnetiP26187.
PhosphoSitePlusiP26187.

Expressioni

Gene expression databases

BgeeiENSMUSG00000054612.
CleanExiMM_MGMT.
GenevisibleiP26187. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080224.

Structurei

3D structure databases

ProteinModelPortaliP26187.
SMRiP26187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP26187.
KOiK00567.
OMAiMDKACEM.
OrthoDBiEOG091G11SR.
PhylomeDBiP26187.
TreeFamiTF314064.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

P26187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP
60 70 80 90 100
EVLGGPEGVP EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR
110 120 130 140 150
QVLWKLLKVV KFGETVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP TGQPASKGLG LTGTWLKSSF
210
ESTSSEPSGR N
Length:211
Mass (Da):22,435
Last modified:January 23, 2007 - v3
Checksum:i838BB92B9A1083D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
CCDSiCCDS21945.1.
PIRiA41809.
RefSeqiNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.3.
XP_006507473.1. XM_006507410.3.
UniGeneiMm.440219.

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneIDi17314.
KEGGimmu:17314.
UCSCiuc009keq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
CCDSiCCDS21945.1.
PIRiA41809.
RefSeqiNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.3.
XP_006507473.1. XM_006507410.3.
UniGeneiMm.440219.

3D structure databases

ProteinModelPortaliP26187.
SMRiP26187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080224.

PTM databases

iPTMnetiP26187.
PhosphoSitePlusiP26187.

Proteomic databases

EPDiP26187.
MaxQBiP26187.
PaxDbiP26187.
PeptideAtlasiP26187.
PRIDEiP26187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneIDi17314.
KEGGimmu:17314.
UCSCiuc009keq.1. mouse.

Organism-specific databases

CTDi4255.
MGIiMGI:96977. Mgmt.

Phylogenomic databases

eggNOGiKOG4062. Eukaryota.
COG0350. LUCA.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP26187.
KOiK00567.
OMAiMDKACEM.
OrthoDBiEOG091G11SR.
PhylomeDBiP26187.
TreeFamiTF314064.

Miscellaneous databases

ChiTaRSiMgmt. mouse.
PROiPR:P26187.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054612.
CleanExiMM_MGMT.
GenevisibleiP26187. MM.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMGMT_MOUSE
AccessioniPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.