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P26187

- MGMT_MOUSE

UniProt

P26187 - MGMT_MOUSE

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Protein
Methylated-DNA--protein-cysteine methyltransferase
Gene
Mgmt
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Cofactori

Binds 1 zinc ion By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Zinc By similarity
Metal bindingi24 – 241Zinc By similarity
Metal bindingi29 – 291Zinc By similarity
Metal bindingi89 – 891Zinc By similarity
Binding sitei99 – 991DNA; via amide nitrogen By similarity
Binding sitei118 – 1181DNA By similarity
Binding sitei119 – 1191DNA; via amide nitrogen By similarity
Binding sitei127 – 1271DNA By similarity
Binding sitei132 – 1321DNA By similarity
Active sitei149 – 1491Nucleophile; methyl group acceptor By similarity
Binding sitei155 – 1551DNA; via amide nitrogen By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. calcium ion binding Source: Ensembl
  3. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: UniProtKB-EC
  4. methyltransferase activity Source: MGI

GO - Biological processi

  1. DNA dealkylation involved in DNA repair Source: MGI
  2. DNA repair Source: MGI
  3. cellular response to DNA damage stimulus Source: MGI
  4. cellular response to ionizing radiation Source: Ensembl
  5. cellular response to organic cyclic compound Source: Ensembl
  6. cellular response to oxidative stress Source: Ensembl
  7. mammary gland epithelial cell differentiation Source: Ensembl
  8. negative regulation of cell death Source: Ensembl
  9. positive regulation of DNA repair Source: Ensembl
  10. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  11. response to drug Source: Ensembl
  12. response to ethanol Source: Ensembl
  13. response to folic acid Source: Ensembl
  14. response to toxic substance Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:96977. Mgmt.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Methylated-DNA--protein-cysteine methyltransferase
PRO_0000139360Add
BLAST

Proteomic databases

MaxQBiP26187.
PaxDbiP26187.
PRIDEiP26187.

PTM databases

PhosphoSiteiP26187.

Expressioni

Gene expression databases

BgeeiP26187.
CleanExiMM_MGMT.
GenevestigatoriP26187.

Structurei

3D structure databases

ProteinModelPortaliP26187.
SMRiP26187. Positions 6-178.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.

Phylogenomic databases

eggNOGiCOG0350.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP26187.
KOiK00567.
OMAiQQDSFTR.
OrthoDBiEOG7RRF9D.
PhylomeDBiP26187.
TreeFamiTF314064.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26187-1 [UniParc]FASTAAdd to Basket

« Hide

MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP    50
EVLGGPEGVP EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR 100
QVLWKLLKVV KFGETVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH 150
RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP TGQPASKGLG LTGTWLKSSF 200
ESTSSEPSGR N 211
Length:211
Mass (Da):22,435
Last modified:January 23, 2007 - v3
Checksum:i838BB92B9A1083D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
CCDSiCCDS21945.1.
PIRiA41809.
RefSeqiNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.1.
XP_006507473.1. XM_006507410.1.
UniGeneiMm.440219.

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneIDi17314.
KEGGimmu:17314.
UCSCiuc009keq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84524 mRNA. Translation: AAA37126.1 .
AB092489 mRNA. Translation: BAC16763.1 .
AB092490 mRNA. Translation: BAC16764.1 .
BC031888 mRNA. Translation: AAH31888.1 .
CCDSi CCDS21945.1.
PIRi A41809.
RefSeqi NP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.1.
XP_006507473.1. XM_006507410.1.
UniGenei Mm.440219.

3D structure databases

ProteinModelPortali P26187.
SMRi P26187. Positions 6-178.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P26187.

Proteomic databases

MaxQBi P26187.
PaxDbi P26187.
PRIDEi P26187.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081510 ; ENSMUSP00000080224 ; ENSMUSG00000054612 .
GeneIDi 17314.
KEGGi mmu:17314.
UCSCi uc009keq.1. mouse.

Organism-specific databases

CTDi 4255.
MGIi MGI:96977. Mgmt.

Phylogenomic databases

eggNOGi COG0350.
GeneTreei ENSGT00390000015799.
HOGENOMi HOG000244137.
HOVERGENi HBG001146.
InParanoidi P26187.
KOi K00567.
OMAi QQDSFTR.
OrthoDBi EOG7RRF9D.
PhylomeDBi P26187.
TreeFami TF314064.

Miscellaneous databases

NextBioi 291876.
PROi P26187.
SOURCEi Search...

Gene expression databases

Bgeei P26187.
CleanExi MM_MGMT.
Genevestigatori P26187.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view ]
SUPFAMi SSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsi TIGR00589. ogt. 1 hit.
PROSITEi PS00374. MGMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli."
    Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S.
    Biochemistry 31:1897-1903(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "MGMT sequence of B6 and C3H mice."
    Shimada Y., Kakinuma S., Kubo A., Nishimura M.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H and C57BL/6.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.

Entry informationi

Entry nameiMGMT_MOUSE
AccessioniPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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