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P26187 (MGMT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylated-DNA--protein-cysteine methyltransferase

EC=2.1.1.63
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene names
Name:Mgmt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activity

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Nucleus Probable.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Sequence similarities

Belongs to the MGMT family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processDNA dealkylation involved in DNA repair

Inferred from direct assay PubMed 1554415. Source: MGI

DNA repair

Inferred from mutant phenotype PubMed 13679151. Source: MGI

cellular response to DNA damage stimulus

Inferred from mutant phenotype PubMed 10657972PubMed 9560238. Source: MGI

cellular response to ionizing radiation

Inferred from electronic annotation. Source: Ensembl

cellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

cellular response to oxidative stress

Inferred from electronic annotation. Source: Ensembl

mammary gland epithelial cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell death

Inferred from electronic annotation. Source: Ensembl

positive regulation of DNA repair

Inferred from electronic annotation. Source: Ensembl

regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from mutant phenotype PubMed 13679151. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to folic acid

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

calcium ion binding

Inferred from electronic annotation. Source: Ensembl

methylated-DNA-[protein]-cysteine S-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

methyltransferase activity

Inferred from direct assay PubMed 1554415PubMed 9560238. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Methylated-DNA--protein-cysteine methyltransferase
PRO_0000139360

Sites

Active site1491Nucleophile; methyl group acceptor By similarity
Metal binding51Zinc By similarity
Metal binding241Zinc By similarity
Metal binding291Zinc By similarity
Metal binding891Zinc By similarity
Binding site991DNA; via amide nitrogen By similarity
Binding site1181DNA By similarity
Binding site1191DNA; via amide nitrogen By similarity
Binding site1271DNA By similarity
Binding site1321DNA By similarity
Binding site1551DNA; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P26187 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 838BB92B9A1083D6

FASTA21122,435
        10         20         30         40         50         60 
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP EVLGGPEGVP 

        70         80         90        100        110        120 
EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGETVSYQQ 

       130        140        150        160        170        180 
LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP 

       190        200        210 
TGQPASKGLG LTGTWLKSSF ESTSSEPSGR N 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli."
Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S.
Biochemistry 31:1897-1903(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"MGMT sequence of B6 and C3H mice."
Shimada Y., Kakinuma S., Kubo A., Nishimura M.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H and C57BL/6.
Tissue: Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
PIRA41809.
RefSeqNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.1.
XP_006507473.1. XM_006507410.1.
UniGeneMm.440219.

3D structure databases

ProteinModelPortalP26187.
SMRP26187. Positions 6-178.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP26187.

Proteomic databases

PaxDbP26187.
PRIDEP26187.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneID17314.
KEGGmmu:17314.
UCSCuc009keq.1. mouse.

Organism-specific databases

CTD4255.
MGIMGI:96977. Mgmt.

Phylogenomic databases

eggNOGCOG0350.
GeneTreeENSGT00390000015799.
HOGENOMHOG000244137.
HOVERGENHBG001146.
InParanoidP26187.
KOK00567.
OMAQQDSFTR.
OrthoDBEOG7RRF9D.
PhylomeDBP26187.
TreeFamTF314064.

Gene expression databases

BgeeP26187.
CleanExMM_MGMT.
GenevestigatorP26187.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsTIGR00589. ogt. 1 hit.
PROSITEPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291876.
PROP26187.
SOURCESearch...

Entry information

Entry nameMGMT_MOUSE
AccessionPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot