P26187 (MGMT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63

Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:

Mgmt

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Nucleus Probable.
Miscellaneous	This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA dealkylation involved in DNA repair Inferred from direct assay PubMed 1554415. Source: MGI cellular response to ionizing radiation Inferred from electronic annotation. Source: Compara cellular response to organic cyclic compound Inferred from electronic annotation. Source: Compara cellular response to oxidative stress Inferred from electronic annotation. Source: Compara mammary gland epithelial cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from electronic annotation. Source: Compara positive regulation of DNA repair Inferred from electronic annotation. Source: Compara regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from mutant phenotype PubMed 13679151. Source: MGI response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to folic acid Inferred from electronic annotation. Source: Compara response to toxic substance Inferred from electronic annotation. Source: Compara
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: Compara methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC methyltransferase activity Inferred from direct assay PubMed 1554415PubMed 9560238. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Methylated-DNA--protein-cysteine methyltransferase		PRO_0000139360
Sites
	Active site	149	1	Nucleophile; methyl group acceptor By similarity
	Metal binding	5	1	Zinc By similarity
	Metal binding	24	1	Zinc By similarity
	Metal binding	29	1	Zinc By similarity
	Metal binding	89	1	Zinc By similarity
	Binding site	99	1	DNA; via amide nitrogen By similarity
	Binding site	118	1	DNA By similarity
	Binding site	119	1	DNA; via amide nitrogen By similarity
	Binding site	127	1	DNA By similarity
	Binding site	132	1	DNA By similarity
	Binding site	155	1	DNA; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26187 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 838BB92B9A1083D6	FASTA	211	22,435
	10 20 30 40 50 60 MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP EVLGGPEGVP 70 80 90 100 110 120 EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGETVSYQQ 130 140 150 160 170 180 LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP 190 200 210 TGQPASKGLG LTGTWLKSSF ESTSSEPSGR N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli." Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S. Biochemistry 31:1897-1903(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"MGMT sequence of B6 and C3H mice." Shimada Y., Kakinuma S., Kubo A., Nishimura M. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H and C57BL/6. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84524 mRNA. Translation: AAA37126.1. AB092489 mRNA. Translation: BAC16763.1. AB092490 mRNA. Translation: BAC16764.1. BC031888 mRNA. Translation: AAH31888.1.
IPI	IPI00229662.
PIR	A41809.
RefSeq	NP_032624.1. NM_008598.2.
UniGene	Mm.440219.
3D structure databases
ProteinModelPortal	P26187.
SMR	P26187. Positions 6-178.
ModBase	Search...
PTM databases
PhosphoSite	P26187.
Proteomic databases
PaxDb	P26187.
PRIDE	P26187.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneID	17314.
KEGG	mmu:17314.
UCSC	uc009keq.1. mouse.
Organism-specific databases
CTD	4255.
MGI	MGI:96977. Mgmt.
Phylogenomic databases
eggNOG	COG0350.
GeneTree	ENSGT00390000015799.
HOGENOM	HOG000244137.
HOVERGEN	HBG001146.
InParanoid	P26187.
KO	K00567.
OMA	HLKQWLL.
OrthoDB	EOG4SJ5G1.
Gene expression databases
Bgee	P26187.
CleanEx	MM_MGMT.
Genevestigator	P26187.
GermOnline	ENSMUSG00000054612. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR008332. MethylG_MeTrfase. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
SUPFAM	SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291876.
SOURCE	Search...

Entry information

Entry name

MGMT_MOUSE

Accession

Primary (citable) accession number: P26187
Secondary accession number(s): Q54A49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 104 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program