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P26187

- MGMT_MOUSE

UniProt

P26187 - MGMT_MOUSE

Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

    Catalytic activityi

    DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

    Cofactori

    Binds 1 zinc ion.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi5 – 51ZincBy similarity
    Metal bindingi24 – 241ZincBy similarity
    Metal bindingi29 – 291ZincBy similarity
    Metal bindingi89 – 891ZincBy similarity
    Binding sitei99 – 991DNA; via amide nitrogenBy similarity
    Binding sitei118 – 1181DNABy similarity
    Binding sitei119 – 1191DNA; via amide nitrogenBy similarity
    Binding sitei127 – 1271DNABy similarity
    Binding sitei132 – 1321DNABy similarity
    Active sitei149 – 1491Nucleophile; methyl group acceptorPROSITE-ProRule annotation
    Binding sitei155 – 1551DNA; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: Ensembl
    2. DNA binding Source: UniProtKB-KW
    3. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: UniProtKB-EC
    4. methyltransferase activity Source: MGI

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: MGI
    2. cellular response to ionizing radiation Source: Ensembl
    3. cellular response to organic cyclic compound Source: Ensembl
    4. cellular response to oxidative stress Source: Ensembl
    5. DNA dealkylation involved in DNA repair Source: MGI
    6. DNA repair Source: MGI
    7. mammary gland epithelial cell differentiation Source: Ensembl
    8. negative regulation of cell death Source: Ensembl
    9. positive regulation of DNA repair Source: Ensembl
    10. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    11. response to drug Source: Ensembl
    12. response to ethanol Source: Ensembl
    13. response to folic acid Source: Ensembl
    14. response to toxic substance Source: Ensembl

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
    Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
    Short name:
    MGMT
    O-6-methylguanine-DNA-alkyltransferase
    Gene namesi
    Name:Mgmt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:96977. Mgmt.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211Methylated-DNA--protein-cysteine methyltransferasePRO_0000139360Add
    BLAST

    Proteomic databases

    MaxQBiP26187.
    PaxDbiP26187.
    PRIDEiP26187.

    PTM databases

    PhosphoSiteiP26187.

    Expressioni

    Gene expression databases

    BgeeiP26187.
    CleanExiMM_MGMT.
    GenevestigatoriP26187.

    Structurei

    3D structure databases

    ProteinModelPortaliP26187.
    SMRiP26187. Positions 6-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MGMT family.Curated

    Phylogenomic databases

    eggNOGiCOG0350.
    GeneTreeiENSGT00390000015799.
    HOGENOMiHOG000244137.
    HOVERGENiHBG001146.
    InParanoidiP26187.
    KOiK00567.
    OMAiQQDSFTR.
    OrthoDBiEOG7RRF9D.
    PhylomeDBiP26187.
    TreeFamiTF314064.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
    IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
    IPR008332. MethylG_MeTrfase_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF01035. DNA_binding_1. 1 hit.
    PF02870. Methyltransf_1N. 1 hit.
    [Graphical view]
    SUPFAMiSSF46767. SSF46767. 1 hit.
    SSF53155. SSF53155. 1 hit.
    TIGRFAMsiTIGR00589. ogt. 1 hit.
    PROSITEiPS00374. MGMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26187-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP    50
    EVLGGPEGVP EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR 100
    QVLWKLLKVV KFGETVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH 150
    RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP TGQPASKGLG LTGTWLKSSF 200
    ESTSSEPSGR N 211
    Length:211
    Mass (Da):22,435
    Last modified:January 23, 2007 - v3
    Checksum:i838BB92B9A1083D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84524 mRNA. Translation: AAA37126.1.
    AB092489 mRNA. Translation: BAC16763.1.
    AB092490 mRNA. Translation: BAC16764.1.
    BC031888 mRNA. Translation: AAH31888.1.
    CCDSiCCDS21945.1.
    PIRiA41809.
    RefSeqiNP_032624.1. NM_008598.2.
    XP_006507472.1. XM_006507409.1.
    XP_006507473.1. XM_006507410.1.
    UniGeneiMm.440219.

    Genome annotation databases

    EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
    GeneIDi17314.
    KEGGimmu:17314.
    UCSCiuc009keq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84524 mRNA. Translation: AAA37126.1 .
    AB092489 mRNA. Translation: BAC16763.1 .
    AB092490 mRNA. Translation: BAC16764.1 .
    BC031888 mRNA. Translation: AAH31888.1 .
    CCDSi CCDS21945.1.
    PIRi A41809.
    RefSeqi NP_032624.1. NM_008598.2.
    XP_006507472.1. XM_006507409.1.
    XP_006507473.1. XM_006507410.1.
    UniGenei Mm.440219.

    3D structure databases

    ProteinModelPortali P26187.
    SMRi P26187. Positions 6-178.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P26187.

    Proteomic databases

    MaxQBi P26187.
    PaxDbi P26187.
    PRIDEi P26187.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081510 ; ENSMUSP00000080224 ; ENSMUSG00000054612 .
    GeneIDi 17314.
    KEGGi mmu:17314.
    UCSCi uc009keq.1. mouse.

    Organism-specific databases

    CTDi 4255.
    MGIi MGI:96977. Mgmt.

    Phylogenomic databases

    eggNOGi COG0350.
    GeneTreei ENSGT00390000015799.
    HOGENOMi HOG000244137.
    HOVERGENi HBG001146.
    InParanoidi P26187.
    KOi K00567.
    OMAi QQDSFTR.
    OrthoDBi EOG7RRF9D.
    PhylomeDBi P26187.
    TreeFami TF314064.

    Miscellaneous databases

    NextBioi 291876.
    PROi P26187.
    SOURCEi Search...

    Gene expression databases

    Bgeei P26187.
    CleanExi MM_MGMT.
    Genevestigatori P26187.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR001497. MethylDNA_cys_MeTrfase_AS.
    IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
    IPR008332. MethylG_MeTrfase_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF01035. DNA_binding_1. 1 hit.
    PF02870. Methyltransf_1N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46767. SSF46767. 1 hit.
    SSF53155. SSF53155. 1 hit.
    TIGRFAMsi TIGR00589. ogt. 1 hit.
    PROSITEi PS00374. MGMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli."
      Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S.
      Biochemistry 31:1897-1903(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "MGMT sequence of B6 and C3H mice."
      Shimada Y., Kakinuma S., Kubo A., Nishimura M.
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H and C57BL/6.
      Tissue: Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiMGMT_MOUSE
    AccessioniPrimary (citable) accession number: P26187
    Secondary accession number(s): Q54A49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 114 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3