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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

Mgmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51ZincBy similarity
Metal bindingi24 – 241ZincBy similarity
Metal bindingi29 – 291ZincBy similarity
Metal bindingi89 – 891ZincBy similarity
Binding sitei99 – 991DNA; via amide nitrogenBy similarity
Binding sitei118 – 1181DNABy similarity
Binding sitei119 – 1191DNA; via amide nitrogenBy similarity
Binding sitei127 – 1271DNABy similarity
Binding sitei132 – 1321DNABy similarity
Active sitei149 – 1491Nucleophile; methyl group acceptorPROSITE-ProRule annotation
Binding sitei155 – 1551DNA; via amide nitrogenBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. damaged DNA binding Source: GO_Central
  3. methylated-DNA-[protein]-cysteine S-methyltransferase activity Source: GO_Central
  4. methyltransferase activity Source: MGI

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: MGI
  2. cellular response to ionizing radiation Source: Ensembl
  3. cellular response to organic cyclic compound Source: Ensembl
  4. cellular response to oxidative stress Source: Ensembl
  5. DNA dealkylation involved in DNA repair Source: MGI
  6. DNA repair Source: MGI
  7. mammary gland epithelial cell differentiation Source: Ensembl
  8. negative regulation of cell death Source: Ensembl
  9. positive regulation of DNA repair Source: Ensembl
  10. regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  11. response to drug Source: Ensembl
  12. response to ethanol Source: Ensembl
  13. response to folic acid Source: Ensembl
  14. response to toxic substance Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_287093. DNA Damage Reversal.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase (EC:2.1.1.63)
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name:
MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene namesi
Name:Mgmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96977. Mgmt.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. membrane Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Methylated-DNA--protein-cysteine methyltransferasePRO_0000139360Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei205 – 2051PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP26187.
PaxDbiP26187.
PRIDEiP26187.

PTM databases

PhosphoSiteiP26187.

Expressioni

Gene expression databases

BgeeiP26187.
CleanExiMM_MGMT.
GenevestigatoriP26187.

Structurei

3D structure databases

ProteinModelPortaliP26187.
SMRiP26187. Positions 6-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiCOG0350.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP26187.
KOiK00567.
OMAiQQDSFTR.
OrthoDBiEOG7RRF9D.
PhylomeDBiP26187.
TreeFamiTF314064.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP
60 70 80 90 100
EVLGGPEGVP EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR
110 120 130 140 150
QVLWKLLKVV KFGETVSYQQ LAALAGNPKA ARAVGGAMRS NPVPILIPCH
160 170 180 190 200
RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP TGQPASKGLG LTGTWLKSSF
210
ESTSSEPSGR N
Length:211
Mass (Da):22,435
Last modified:January 23, 2007 - v3
Checksum:i838BB92B9A1083D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
CCDSiCCDS21945.1.
PIRiA41809.
RefSeqiNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.2.
XP_006507473.1. XM_006507410.2.
UniGeneiMm.440219.

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneIDi17314.
KEGGimmu:17314.
UCSCiuc009keq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84524 mRNA. Translation: AAA37126.1.
AB092489 mRNA. Translation: BAC16763.1.
AB092490 mRNA. Translation: BAC16764.1.
BC031888 mRNA. Translation: AAH31888.1.
CCDSiCCDS21945.1.
PIRiA41809.
RefSeqiNP_032624.1. NM_008598.2.
XP_006507472.1. XM_006507409.2.
XP_006507473.1. XM_006507410.2.
UniGeneiMm.440219.

3D structure databases

ProteinModelPortaliP26187.
SMRiP26187. Positions 6-178.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP26187.

Proteomic databases

MaxQBiP26187.
PaxDbiP26187.
PRIDEiP26187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081510; ENSMUSP00000080224; ENSMUSG00000054612.
GeneIDi17314.
KEGGimmu:17314.
UCSCiuc009keq.1. mouse.

Organism-specific databases

CTDi4255.
MGIiMGI:96977. Mgmt.

Phylogenomic databases

eggNOGiCOG0350.
GeneTreeiENSGT00390000015799.
HOGENOMiHOG000244137.
HOVERGENiHBG001146.
InParanoidiP26187.
KOiK00567.
OMAiQQDSFTR.
OrthoDBiEOG7RRF9D.
PhylomeDBiP26187.
TreeFamiTF314064.

Enzyme and pathway databases

ReactomeiREACT_287093. DNA Damage Reversal.

Miscellaneous databases

ChiTaRSiMgmt. mouse.
NextBioi291876.
PROiP26187.
SOURCEiSearch...

Gene expression databases

BgeeiP26187.
CleanExiMM_MGMT.
GenevestigatoriP26187.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli."
    Shiota S., von Wronski M.A., Tano K., Bigner D.D., Brent T.P., Mitra S.
    Biochemistry 31:1897-1903(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "MGMT sequence of B6 and C3H mice."
    Shimada Y., Kakinuma S., Kubo A., Nishimura M.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H and C57BL/6.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.

Entry informationi

Entry nameiMGMT_MOUSE
AccessioniPrimary (citable) accession number: P26187
Secondary accession number(s): Q54A49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.