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P26186 (MGMT_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylated-DNA--protein-cysteine methyltransferase
EC=2.1.1.63
Alternative name(s):
6-O-methylguanine-DNA methyltransferase
Short name=MGMT
O-6-methylguanine-DNA-alkyltransferase
Gene names
Name:MGMT
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Catalytic activity

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Nucleus Probable.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.

Sequence similarities

Belongs to the MGMT family.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methylated-DNA-[protein]-cysteine S-methyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Methylated-DNA--protein-cysteine methyltransferase
PRO_0000139358

Sites

Active site1491Nucleophile; methyl group acceptor By similarity
Metal binding51Zinc By similarity
Metal binding241Zinc By similarity
Metal binding291Zinc By similarity
Metal binding891Zinc By similarity
Binding site991DNA; via amide nitrogen By similarity
Binding site1181DNA By similarity
Binding site1191DNA; via amide nitrogen By similarity
Binding site1271DNA By similarity
Binding site1321DNA By similarity
Binding site1551DNA; via amide nitrogen By similarity

Amino acid modifications

Modified residue101Phosphothreonine By similarity
Modified residue2051Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P26186 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 38CD50618BAB8A8D

FASTA20922,333
        10         20         30         40         50         60 
MAETCKMKYT VFHSPLGKIE LCGCERGLHG IRFLSGKTPS SDPKEAPASP ELLGGPEDLP 

        70         80         90        100        110        120 
ESLVQCTTWL EAYFQEPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGEMVSYQQ 

       130        140        150        160        170        180 
LAALAGNPKA ARAVGGAMRN NPVPILIPCH RVICSNGSIG NYSGGGQAVK EWLLAHEGIP 

       190        200 
TRQPACKDLG LTGTRLKPSG GSTSSKLSG

« Hide

References

[1]"Isolation and partial characterisation of a Chinese hamster O6-alkylguanine-DNA alkyltransferase cDNA."
Rafferty J.A., Elder R.H., Watson A.J., Cawkwell L., Potter P.M., Margison G.P.
Nucleic Acids Res. 20:1891-1895(1992) [PubMed: 1579490] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X65081 mRNA. Translation: CAA46209.1.

3D structure databases

ProteinModelPortalP26186.
SMRP26186. Positions 6-178.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG001146.

Family and domain databases

InterProIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PfamPF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
[Graphical view]
SUPFAMSSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
TIGRFAMsTIGR00589. Ogt. 1 hit.
PROSITEPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMGMT_CRIGR
AccessionPrimary (citable) accession number: P26186
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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