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Protein

Light-independent protochlorophyllide reductase subunit N

Gene

bchN

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (BchN-BchB) is the catalytic component of the complex.UniRule annotation2 Publications

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotation1 PublicationNote: Binds 1 [4Fe-4S] cluster per heterodimer (PubMed:20400946). The cluster is bound at the heterodimer interface by residues from both subunits (PubMed:20400946).UniRule annotation1 Publication

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Iron-sulfur (4Fe-4S); shared with heterodimeric partner1 Publication
Metal bindingi51 – 511Iron-sulfur (4Fe-4S); shared with heterodimeric partner1 Publication
Metal bindingi112 – 1121Iron-sulfur (4Fe-4S); shared with heterodimeric partner1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13269.
RCAP272942:GJIY-678-MONOMER.
BRENDAi1.3.7.7. 5381.
UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit NUniRule annotation (EC:1.3.7.7UniRule annotation1 Publication)
Short name:
DPOR subunit NUniRule annotation
Short name:
LI-POR subunit NUniRule annotation
Gene namesi
Name:bchNUniRule annotation
Ordered Locus Names:RCAP_rcc00665
OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Taxonomic identifieri272942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
Proteomesi
  • UP000002361 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251F → A: Retains 50% activity. 1 Publication
Mutagenesisi26 – 261C → A: Does not form heterotetramers. 1 Publication
Mutagenesisi51 – 511C → A: Does not form heterotetramers. 1 Publication
Mutagenesisi112 – 1121C → A: Does not form heterotetramers. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Light-independent protochlorophyllide reductase subunit NPRO_0000208596Add
BLAST

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB. Forms a heterotetramer of two BchB and two BchN subunits (PubMed:20400946).UniRule annotation1 Publication

Protein-protein interaction databases

DIPiDIP-59277N.
STRINGi272942.RCAP_rcc00665.

Structurei

Secondary structure

1
424
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi15 – 184Combined sources
Helixi27 – 304Combined sources
Helixi31 – 377Combined sources
Beta strandi41 – 477Combined sources
Helixi49 – 5911Combined sources
Helixi60 – 645Combined sources
Beta strandi68 – 736Combined sources
Helixi76 – 794Combined sources
Beta strandi80 – 823Combined sources
Helixi85 – 9814Combined sources
Beta strandi105 – 1106Combined sources
Helixi112 – 1165Combined sources
Helixi121 – 13111Combined sources
Turni133 – 1353Combined sources
Beta strandi137 – 1426Combined sources
Turni145 – 1473Combined sources
Helixi152 – 16110Combined sources
Helixi162 – 1643Combined sources
Beta strandi173 – 1775Combined sources
Helixi181 – 19313Combined sources
Beta strandi199 – 2035Combined sources
Helixi207 – 2093Combined sources
Beta strandi218 – 2236Combined sources
Helixi227 – 2359Combined sources
Beta strandi239 – 2413Combined sources
Helixi248 – 26215Combined sources
Helixi267 – 28620Combined sources
Helixi289 – 2924Combined sources
Beta strandi296 – 2994Combined sources
Beta strandi301 – 3044Combined sources
Helixi306 – 31510Combined sources
Beta strandi320 – 3278Combined sources
Helixi331 – 3388Combined sources
Beta strandi346 – 3505Combined sources
Helixi353 – 36311Combined sources
Beta strandi366 – 3705Combined sources
Helixi372 – 3798Combined sources
Turni380 – 3823Combined sources
Beta strandi385 – 3873Combined sources
Helixi388 – 3914Combined sources
Beta strandi397 – 3993Combined sources
Helixi402 – 42019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AEKX-ray2.30A/C2-424[»]
3AEQX-ray2.90A/C2-424[»]
3AERX-ray2.80A/C2-424[»]
3AESX-ray2.50A/C2-424[»]
3AETX-ray2.91A/C2-424[»]
3AEUX-ray2.90A/C2-424[»]
ProteinModelPortaliP26164.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26164.

Family & Domainsi

Sequence similaritiesi

Belongs to the BchN/ChlN family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C78. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000265994.
KOiK04038.
OMAiREVFCGL.
OrthoDBiPOG091H13ZK.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN. 1 hit.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.

Sequencei

Sequence statusi: Complete.

P26164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLDSPTFGC TDSPVRRERG QKAVFCGLTS IVWLHRKMQD AFFLVVGSRT
60 70 80 90 100
CAHLLQAAAG VMIFAEPRFG TAVLEEQDLA GLADAHKELD REVAKLLERR
110 120 130 140 150
PDIRQLFLVG SCPSEVLKLD LDRAAERLSG LHAPHVRVYS YTGSGLDTTF
160 170 180 190 200
TQGEDTCLAA MVPTLDTTEA AELIVVGALP DVVEDQCLSL LTQLGVGPVR
210 220 230 240 250
MLPARRSDIE PAVGPNTRFI LAQPFLGETT GALERRGAKR IAAPFPFGEE
260 270 280 290 300
GTTLWLKAVA DAYGVSAEKF EAVTAAPRAR AKKAIAAHLE TLTGKSLFMF
310 320 330 340 350
PDSQLEIPLA RFLARECGMK TTEIATPFLH KAIMAPDLAL LPSNTALTEG
360 370 380 390 400
QDLEAQLDRH EAINPDLTVC GLGLANPLEA KGHATKWAIE LVFTPVHFYE
410 420
QAGDLAGLFS RPLRRRALLN GGAA
Length:424
Mass (Da):45,830
Last modified:May 1, 1992 - v1
Checksum:iC90C75233802834D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11165 Genomic DNA. Translation: CAA77526.1.
CP001312 Genomic DNA. Translation: ADE84430.1.
PIRiB49851.
RefSeqiWP_013066409.1. NC_014034.1.

Genome annotation databases

EnsemblBacteriaiADE84430; ADE84430; RCAP_rcc00665.
KEGGircp:RCAP_rcc00665.
PATRICi35501398. VBIRhoCap134200_0676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11165 Genomic DNA. Translation: CAA77526.1.
CP001312 Genomic DNA. Translation: ADE84430.1.
PIRiB49851.
RefSeqiWP_013066409.1. NC_014034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AEKX-ray2.30A/C2-424[»]
3AEQX-ray2.90A/C2-424[»]
3AERX-ray2.80A/C2-424[»]
3AESX-ray2.50A/C2-424[»]
3AETX-ray2.91A/C2-424[»]
3AEUX-ray2.90A/C2-424[»]
ProteinModelPortaliP26164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59277N.
STRINGi272942.RCAP_rcc00665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE84430; ADE84430; RCAP_rcc00665.
KEGGircp:RCAP_rcc00665.
PATRICi35501398. VBIRhoCap134200_0676.

Phylogenomic databases

eggNOGiENOG4105C78. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000265994.
KOiK04038.
OMAiREVFCGL.
OrthoDBiPOG091H13ZK.

Enzyme and pathway databases

UniPathwayiUPA00671.
BioCyciMetaCyc:MONOMER-13269.
RCAP272942:GJIY-678-MONOMER.
BRENDAi1.3.7.7. 5381.

Miscellaneous databases

EvolutionaryTraceiP26164.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN. 1 hit.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBCHN_RHOCB
AccessioniPrimary (citable) accession number: P26164
Secondary accession number(s): D5ANS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.