Reviewed, UniProtKB/Swiss-Prot P26164 (BCHN_RHOCA)

Names and origin

Protein names

Recommended name:
    Light-independent protochlorophyllide reductase subunit N
      Short name=LI-POR subunit N
      Short name=DPOR subunit N
    EC=1.18.-.-

Gene names

Name:

bchN

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). The bchN-bchB pair binds Pchlide. This reaction is light-independent. HAMAP MF_00352
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_00352
Subunit structure	Protochlorophyllide reductase is thought to be composed of three subunits; bchL, bchN and bchB. Could form a heterotetramer of two bchB and two bchN subunits. HAMAP MF_00352
Sequence similarities	Belongs to the bchN/chlN family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity, acting on iron-sulfur proteins as donors Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Light-independent protochlorophyllide reductase subunit N HAMAP MF_00352		PRO_0000208596

Sequences

	Sequence		Length	Mass (Da)
	P26164-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: C90C75233802834D	FASTA	424	45,830
	10 20 30 40 50 60 MSLDSPTFGC TDSPVRRERG QKAVFCGLTS IVWLHRKMQD AFFLVVGSRT CAHLLQAAAG 70 80 90 100 110 120 VMIFAEPRFG TAVLEEQDLA GLADAHKELD REVAKLLERR PDIRQLFLVG SCPSEVLKLD 130 140 150 160 170 180 LDRAAERLSG LHAPHVRVYS YTGSGLDTTF TQGEDTCLAA MVPTLDTTEA AELIVVGALP 190 200 210 220 230 240 DVVEDQCLSL LTQLGVGPVR MLPARRSDIE PAVGPNTRFI LAQPFLGETT GALERRGAKR 250 260 270 280 290 300 IAAPFPFGEE GTTLWLKAVA DAYGVSAEKF EAVTAAPRAR AKKAIAAHLE TLTGKSLFMF 310 320 330 340 350 360 PDSQLEIPLA RFLARECGMK TTEIATPFLH KAIMAPDLAL LPSNTALTEG QDLEAQLDRH 370 380 390 400 410 420 EAINPDLTVC GLGLANPLEA KGHATKWAIE LVFTPVHFYE QAGDLAGLFS RPLRRRALLN GGAA

References

[1]	"bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants." Burke D.H., Alberti M., Hearst J.E. J. Bacteriol. 175:2414-2422(1993) [PubMed: 8385667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23782 / LMG 2373 / NCIB 11773 / SB1003 / St. Louis.
[2]	"Reconstitution of light-independent protochlorophyllide reductase from purified bchL and bchN-bchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme." Fujita Y., Bauer C.E. J. Biol. Chem. 275:23583-23588(2000) [PubMed: 10811655] [Abstract] Cited for: CHARACTERIZATION. Strain: SB1003 / CB1029.
[3]	Fujita Y. Unpublished observations (JUL-2001) Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11165 Genomic DNA. Translation: CAA77526.1.
PIR	B49851.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13269.
Family and domain databases
HAMAP	MF_00352. ChlN_BchN. [Tree]
InterPro	IPR000510. Nase/OxRdtase_comp1. IPR005970. Protochl_reductN. [Graphical view]
Pfam	PF00148. Oxidored_nitro. 1 hit. [Graphical view]
PIRSF	PIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMs	TIGR01279. DPOR_bchN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCHN_RHOCA

Accession

Primary (citable) accession number: P26164

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)