P26163 (BCHB_RHOCB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Light-independent protochlorophyllide reductase subunit B
Short name=DPOR subunit B
Short name=LI-POR subunit B
EC=1.18.-.-

Gene names

Name:	bchB
Synonyms:	bchK
Ordered Locus Names:	RCAP_rcc00664

Organism

Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) [Complete proteome] [HAMAP]

Taxonomic identifier

272942 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). The BchN-BchB pair binds Pchlide. This reaction is light-independent. HAMAP MF_00353
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_00353
Subunit structure	Protochlorophyllide reductase is thought to be composed of three subunits; BchL, BchN and BchB. Could form a heterotetramer of two BchB and two BchN subunits.
Sequence similarities	Belongs to the ChlB/BchB/BchZ family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity, acting on iron-sulfur proteins as donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 525

525

Light-independent protochlorophyllide reductase subunit B HAMAP MF_00353

PRO_0000219800

Secondary structure

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525

Helix Strand Turn

Details...

Helix

17 – 20

4

Beta strand

23 – 31

9

Helix

37 – 39

3

Helix

40 – 45

6

Beta strand

54 – 57

4

Helix

62 – 64

3

Helix

68 – 84

17

Beta strand

87 – 93

7

Helix

97 – 99

3

Helix

108 – 111

4

Beta strand

117 – 119

3

Turn

124 – 126

3

Helix

134 – 144

11

Beta strand

155 – 159

5

Helix

168 – 180

13

Beta strand

185 – 189

5

Helix

196 – 200

5

Beta strand

206 – 210

5

Helix

218 – 226

9

Helix

242 – 252

11

Helix

266 – 271

6

Helix

273 – 278

6

Beta strand

282 – 285

4

Helix

291 – 301

11

Helix

315 – 317

3

Helix

318 – 327

10

Helix

338 – 348

11

Beta strand

351 – 354

4

Helix

357 – 365

9

Beta strand

374 – 376

3

Helix

379 – 381

3

Helix

396 – 403

8

Helix

411 – 417

7

Sequences

	Sequence		Length	Mass (Da)
	P26163 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 4322A6D9F535C3F5	FASTA	525	57,192
	10 20 30 40 50 60 MKLTLWTYEG PPHVGAMRVA TAMKDLQLVL HGPQGDTYAD LLFTMIERRN ARPPVSFSTF 70 80 90 100 110 120 EASHMGTDTA ILLKDALAAA HARYKPQAMA VALTCTAELL QDDPNGISRA LNLPVPVVPL 130 140 150 160 170 180 ELPSYSRKEN YGADETFRAL VRALAVPMER TPEVTCNLLG ATALGFRHRD DVAEVTKLLA 190 200 210 220 230 240 TMGIKVNVCA PLGASPDDLR KLGQAHFNVL MYPETGESAA RHLERACKQP FTKIVPIGVG 250 260 270 280 290 300 ATRDFLAEVS KITGLPVVTD ESTLRQPWWS ASVDSTYLTG KRVFIFGDGT HVIAAARIAA 310 320 330 340 350 360 KEVGFEVVGM GCYNREMARP LRTAAAEYGL EALITDDYLE VEKAIEAAAP ELILGTQMER 370 380 390 400 410 420 NIAKKLGLPC AVISAPVHVQ DFPARYAPQM GFEGANVLFD TWVHPLVMGL EEHLLTMFRE 430 440 450 460 470 480 DFEFHDAAGA SHHGGKAVAR EESPVAPADL APAATSDTPA APSPVVVTQA SGEIRWMPEA 490 500 510 520 ERELRKIPFF VRGKAKRNTE LYAAHKGVCD ITVETLYEAK AHYAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants." Burke D.H., Alberti M., Hearst J.E. J. Bacteriol. 175:2414-2422(1993) [PubMed: 8385667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[2]	"Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003." Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R. J. Bacteriol. 192:3545-3546(2010) [PubMed: 20418398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[3]	"Reconstitution of light-independent protochlorophyllide reductase from purified bchL and bchN-bchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme." Fujita Y., Bauer C.E. J. Biol. Chem. 275:23583-23588(2000) [PubMed: 10811655] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION. Strain: SB1003 / CB1029.
[4]	Fujita Y. Unpublished observations (JUL-2001) Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z11165 Genomic DNA. Translation: CAA77525.1.
CP001312 Genomic DNA. Translation: ADE84429.1.

PIR

C49851.

RefSeq

YP_003576836.1. NC_014034.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3AEK	X-ray	2.30	B/D	1-525	[»]
3AEQ	X-ray	2.90	B/D	1-525	[»]
3AER	X-ray	2.80	B/D	1-525	[»]
3AES	X-ray	2.50	B/D	1-525	[»]
3AET	X-ray	2.91	B/D	1-525	[»]
3AEU	X-ray	2.90	B/D	1-525	[»]

ProteinModelPortal

P26163.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9003493.

KEGG

rcp:RCAP_rcc00664.

PATRIC

35501396. VBIRhoCap134200_0675.

Organism-specific databases

CMR

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-13270.

Family and domain databases

HAMAP

MF_00353. ChlB_BchB.
[Tree]

InterPro

IPR000510. Nase/OxRdtase_comp1.
IPR013580. P-CP/CP_red_C.
IPR005969. Protochl_reductB.
IPR016209. Protochlorophyllide_Rdtase.
[Graphical view]

KO

K04039.

Pfam

PF00148. Oxidored_nitro. 1 hit.
PF08369. PCP_red. 1 hit.
[Graphical view]

PIRSF

PIRSF000163. PCP_ChlB. 1 hit.

TIGRFAMs

TIGR01278. DPOR_BchB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

BCHB_RHOCB

Accession

Primary (citable) accession number: P26163
Secondary accession number(s): D5ANS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program