Reviewed, UniProtKB/Swiss-Prot P26163 (BCHB_RHOCA)

Names and origin

Protein names

Recommended name:
    Light-independent protochlorophyllide reductase subunit B
      Short name=LI-POR subunit B
      Short name=DPOR subunit B
    EC=1.18.-.-

Gene names

Name:	bchB
Synonyms:	bchK

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). The bchN-bchB pair binds Pchlide. This reaction is light-independent. HAMAP MF_00353
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_00353
Subunit structure	Protochlorophyllide reductase is thought to be composed of three subunits; bchL, bchN and bchB. Could form a heterotetramer of two bchB and two bchN subunits. HAMAP MF_00353
Sequence similarities	Belongs to the chlB/bchB/bchZ family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis, dark reaction Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity, acting on iron-sulfur proteins as donors Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Light-independent protochlorophyllide reductase subunit B HAMAP MF_00353		PRO_0000219800

Sequences

	Sequence		Length	Mass (Da)
	P26163-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 4322A6D9F535C3F5	FASTA	525	57,192
	10 20 30 40 50 60 MKLTLWTYEG PPHVGAMRVA TAMKDLQLVL HGPQGDTYAD LLFTMIERRN ARPPVSFSTF 70 80 90 100 110 120 EASHMGTDTA ILLKDALAAA HARYKPQAMA VALTCTAELL QDDPNGISRA LNLPVPVVPL 130 140 150 160 170 180 ELPSYSRKEN YGADETFRAL VRALAVPMER TPEVTCNLLG ATALGFRHRD DVAEVTKLLA 190 200 210 220 230 240 TMGIKVNVCA PLGASPDDLR KLGQAHFNVL MYPETGESAA RHLERACKQP FTKIVPIGVG 250 260 270 280 290 300 ATRDFLAEVS KITGLPVVTD ESTLRQPWWS ASVDSTYLTG KRVFIFGDGT HVIAAARIAA 310 320 330 340 350 360 KEVGFEVVGM GCYNREMARP LRTAAAEYGL EALITDDYLE VEKAIEAAAP ELILGTQMER 370 380 390 400 410 420 NIAKKLGLPC AVISAPVHVQ DFPARYAPQM GFEGANVLFD TWVHPLVMGL EEHLLTMFRE 430 440 450 460 470 480 DFEFHDAAGA SHHGGKAVAR EESPVAPADL APAATSDTPA APSPVVVTQA SGEIRWMPEA 490 500 510 520 ERELRKIPFF VRGKAKRNTE LYAAHKGVCD ITVETLYEAK AHYAR

References

[1]	"bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants." Burke D.H., Alberti M., Hearst J.E. J. Bacteriol. 175:2414-2422(1993) [PubMed: 8385667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23782 / LMG 2373 / NCIB 11773 / SB1003 / St. Louis.
[2]	"Reconstitution of light-independent protochlorophyllide reductase from purified bchL and bchN-bchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme." Fujita Y., Bauer C.E. J. Biol. Chem. 275:23583-23588(2000) [PubMed: 10811655] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION. Strain: SB1003 / CB1029.
[3]	Fujita Y. Unpublished observations (JUL-2001) Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11165 Genomic DNA. Translation: CAA77525.1.
PIR	C49851.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13270.
Family and domain databases
HAMAP	MF_00353. [Tree]
InterPro	IPR000510. Nase/OxRdtase_comp1. IPR013580. PCP_red. IPR005969. Protochl_reductB. IPR016209. Protochlorophyllide_Rdtase. [Graphical view]
Pfam	PF00148. Oxidored_nitro. 1 hit. PF08369. PCP_red. 1 hit. [Graphical view]
PIRSF	PIRSF000163. PCP_ChlB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCHB_RHOCA

Accession

Primary (citable) accession number: P26163

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 15, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)