Skip Header

Contribute Send feedback
Read comments (?) or add your own

P26163 (BCHB_RHOCB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Light-independent protochlorophyllide reductase subunit B

Short name=DPOR subunit B
Short name=LI-POR subunit B
EC=1.18.-.-
Gene names
Name:bchB
Synonyms:bchK
Ordered Locus Names:RCAP_rcc00664
OrganismRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) [Complete proteome] [HAMAP]
Taxonomic identifier272942 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). The BchN-BchB pair binds Pchlide. This reaction is light-independent. HAMAP MF_00353

Pathway

Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_00353

Subunit structure

Protochlorophyllide reductase is thought to be composed of three subunits; BchL, BchN and BchB. Could form a heterotetramer of two BchB and two BchN subunits.

Sequence similarities

Belongs to the ChlB/BchB/BchZ family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Light-independent protochlorophyllide reductase subunit B HAMAP MF_00353
PRO_0000219800

Secondary structure

................................................................. 525
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P26163 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 4322A6D9F535C3F5

FASTA52557,192
        10         20         30         40         50         60 
MKLTLWTYEG PPHVGAMRVA TAMKDLQLVL HGPQGDTYAD LLFTMIERRN ARPPVSFSTF 

        70         80         90        100        110        120 
EASHMGTDTA ILLKDALAAA HARYKPQAMA VALTCTAELL QDDPNGISRA LNLPVPVVPL 

       130        140        150        160        170        180 
ELPSYSRKEN YGADETFRAL VRALAVPMER TPEVTCNLLG ATALGFRHRD DVAEVTKLLA 

       190        200        210        220        230        240 
TMGIKVNVCA PLGASPDDLR KLGQAHFNVL MYPETGESAA RHLERACKQP FTKIVPIGVG 

       250        260        270        280        290        300 
ATRDFLAEVS KITGLPVVTD ESTLRQPWWS ASVDSTYLTG KRVFIFGDGT HVIAAARIAA 

       310        320        330        340        350        360 
KEVGFEVVGM GCYNREMARP LRTAAAEYGL EALITDDYLE VEKAIEAAAP ELILGTQMER 

       370        380        390        400        410        420 
NIAKKLGLPC AVISAPVHVQ DFPARYAPQM GFEGANVLFD TWVHPLVMGL EEHLLTMFRE 

       430        440        450        460        470        480 
DFEFHDAAGA SHHGGKAVAR EESPVAPADL APAATSDTPA APSPVVVTQA SGEIRWMPEA 

       490        500        510        520 
ERELRKIPFF VRGKAKRNTE LYAAHKGVCD ITVETLYEAK AHYAR 

« Hide

References

« Hide 'large scale' references
[1]"bchFNBH bacteriochlorophyll synthesis genes of Rhodobacter capsulatus and identification of the third subunit of light-independent protochlorophyllide reductase in bacteria and plants."
Burke D.H., Alberti M., Hearst J.E.
J. Bacteriol. 175:2414-2422(1993) [PubMed: 8385667] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[2]"Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
J. Bacteriol. 192:3545-3546(2010) [PubMed: 20418398] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
[3]"Reconstitution of light-independent protochlorophyllide reductase from purified bchL and bchN-bchB subunits. In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme."
Fujita Y., Bauer C.E.
J. Biol. Chem. 275:23583-23588(2000) [PubMed: 10811655] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION.
Strain: SB1003 / CB1029.
[4]Fujita Y.
Unpublished observations (JUL-2001)
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11165 Genomic DNA. Translation: CAA77525.1.
CP001312 Genomic DNA. Translation: ADE84429.1.
PIRC49851.
RefSeqYP_003576836.1. NC_014034.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3AEKX-ray2.30B/D1-525[»]
3AEQX-ray2.90B/D1-525[»]
3AERX-ray2.80B/D1-525[»]
3AESX-ray2.50B/D1-525[»]
3AETX-ray2.91B/D1-525[»]
3AEUX-ray2.90B/D1-525[»]
ProteinModelPortalP26163.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9003493.
KEGGrcp:RCAP_rcc00664.
PATRIC35501396. VBIRhoCap134200_0675.

Organism-specific databases

CMRSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13270.

Family and domain databases

HAMAPMF_00353. ChlB_BchB.
[Tree]
InterProIPR000510. Nase/OxRdtase_comp1.
IPR013580. P-CP/CP_red_C.
IPR005969. Protochl_reductB.
IPR016209. Protochlorophyllide_Rdtase.
[Graphical view]
KOK04039.
PfamPF00148. Oxidored_nitro. 1 hit.
PF08369. PCP_red. 1 hit.
[Graphical view]
PIRSFPIRSF000163. PCP_ChlB. 1 hit.
TIGRFAMsTIGR01278. DPOR_BchB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBCHB_RHOCB
AccessionPrimary (citable) accession number: P26163
Secondary accession number(s): D5ANS5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families