P26151 (FCGR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity immunoglobulin gamma Fc receptor I
Short name=IgG Fc receptor I

Alternative name(s):
Fc-gamma RI
Short name=FcRI
CD_antigen=CD64

Gene names

Name:	Fcgr1
Synonyms:	Fcg1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Ref.4 Ref.5
Subunit structure	Interacts with FCERG1; forms a functional signaling complex By similarity. Interacts with FLNA; prevents FCGR1A degradation By similarity. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN By similarity.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Note: Stabilized at the cell membrane through interaction with FCER1G By similarity.
Tissue specificity	Macrophage-specific.
Post-translational modification	N-glycosylated. Ref.3 Phosphorylated on serine residues. Ref.3
Disruption phenotype	According to Ref.4, mice display altered endocytosis of monomeric IgG and impaired antibody-dependent killing of cells by macrophage. Antigen presentation is also affected and those mice develop only reduced inflammatory responses. An increased in IgG responses is also detected associated with an increased number of antibody-forming cells. Ref.5, also reported that a variety of IgG2a-immune complex-dependent immune functions like protection against bacterial infection were impaired. Ref.4 Ref.5
Sequence similarities	Belongs to the immunoglobulin superfamily. FCGR1 family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Caution	It is uncertain whether Met-1 or Met-10 is the initiator.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Cell membrane Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
Ligand	IgG-binding protein
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antibody-dependent cellular cytotoxicity Inferred from mutant phenotype Ref.4Ref.5. Source: MGI antigen processing and presentation of exogenous peptide antigen via MHC class I Inferred from mutant phenotype Ref.5. Source: MGI defense response to bacterium Inferred from mutant phenotype Ref.5. Source: MGI innate immune response Inferred from electronic annotation. Source: UniProtKB-KW phagocytosis, engulfment Inferred from mutant phenotype Ref.4Ref.5PubMed 9551950. Source: MGI phagocytosis, recognition Inferred from mutant phenotype Ref.4Ref.5PubMed 9551950. Source: MGI positive regulation of phagocytosis Inferred from mutant phenotype Ref.4Ref.5PubMed 9551950. Source: MGI positive regulation of type III hypersensitivity Inferred from mutant phenotype Ref.4. Source: MGI positive regulation of type IIa hypersensitivity Inferred from mutant phenotype Ref.5. Source: MGI receptor-mediated endocytosis Inferred from mutant phenotype Ref.4. Source: MGI
Cellular_component	external side of plasma membrane Inferred from direct assay Ref.4. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	IgG binding Inferred from direct assay PubMed 9551950. Source: MGI IgG receptor activity Inferred from mutant phenotype Ref.4Ref.5PubMed 9551950. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 404	380	High affinity immunoglobulin gamma Fc receptor I		PRO_0000015140
Regions
	Topological domain	25 – 297	273	Extracellular Potential
	Transmembrane	298 – 320	23	Helical; Potential
	Topological domain	321 – 404	84	Cytoplasmic Potential
	Domain	32 – 111	80	Ig-like C2-type 1
	Domain	117 – 194	78	Ig-like C2-type 2
	Domain	201 – 286	86	Ig-like C2-type 3
	Region	321 – 342	22	Interaction with EPB41L2 By similarity
Amino acid modifications
	Modified residue	347	1	Phosphoserine Ref.6
	Modified residue	368	1	Phosphothreonine Ref.6
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	168	1	N-linked (GlcNAc...) Potential
	Glycosylation	249	1	N-linked (GlcNAc...) Potential
	Disulfide bond	53 ↔ 95		By similarity
	Disulfide bond	134 ↔ 177		By similarity
	Disulfide bond	221 ↔ 269		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26151 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 1C4F0033842767E7	FASTA	404	44,888
	10 20 30 40 50 60 MILTSFGDDM WLLTTLLLWV PVGGEVVNAT KAVITLQPPW VSIFQKENVT LWCEGPHLPG 70 80 90 100 110 120 DSSTQWFING TAVQISTPSY SIPEASFQDS GEYRCQIGSS MPSDPVQLQI HNDWLLLQAS 130 140 150 160 170 180 RRVLTEGEPL ALRCHGWKNK LVYNVVFYRN GKSFQFSSDS EVAILKTNLS HSGIYHCSGT 190 200 210 220 230 240 GRHRYTSAGV SITVKELFTT PVLRASVSSP FPEGSLVTLN CETNLLLQRP GLQLHFSFYV 250 260 270 280 290 300 GSKILEYRNT SSEYHIARAE REDAGFYWCE VATEDSSVLK RSPELELQVL GPQSSAPVWF 310 320 330 340 350 360 HILFYLSVGI MFSLNTVLYV KIHRLQREKK YNLEVPLVSE QGKKANSFQQ VRSDGVYEEV 370 380 390 400 TATASQTTPK EAPDGPRSSV GDCGPEQPEP LPPSDSTGAQ TSQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of the mouse high affinity Fc receptor for IgG." Sears D.W., Osman N., Tate B., McKenzie I.F.C., Hogarth P.M. J. Immunol. 144:371-378(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure and mapping of the gene encoding mouse high affinity Fc gamma RI and chromosomal location of the human Fc gamma RI gene." Osman N., Kozak C.A., McKenzie I.F.C., Hogarth P.M. J. Immunol. 148:1570-1575(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Biochemical characterization of murine Fc gamma RI." Quilliam A.L., Osman N., McKenzie I.F.C., Hogarth P.M. Immunology 78:358-363(1993) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, PHOSPHORYLATION.
[4]	"FcgammaRI-deficient mice show multiple alterations to inflammatory and immune responses." Barnes N., Gavin A.L., Tan P.S., Mottram P., Koentgen F., Hogarth P.M. Immunity 16:379-389(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]	"FcgammaRI (CD64) contributes substantially to severity of arthritis, hypersensitivity responses, and protection from bacterial infection." Ioan-Facsinay A., de Kimpe S.J., Hellwig S.M.M., van Lent P.L., Hofhuis F.M.A., van Ojik H.H., Sedlik C., da Silveira S.A., Gerber J., de Jong Y.F., Roozendaal R., Aarden L.A., van den Berg W.B., Saito T., Mosser D., Amigorena S., Izui S., van Ommen G.-J.B. , van Vugt M., van de Winkel J.G.J., Verbeek J.S. Immunity 16:391-402(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347 AND THR-368, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31314 mRNA. Translation: AAA40056.1.
IPI	IPI00111856.
PIR	A46480.
RefSeq	NP_034316.1. NM_010186.5.
UniGene	Mm.150.
3D structure databases
ProteinModelPortal	P26151.
SMR	P26151. Positions 31-291.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000029748.
PTM databases
PhosphoSite	P26151.
Proteomic databases
PRIDE	P26151.
Protocols and materials databases
DNASU	14129.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029748; ENSMUSP00000029748; ENSMUSG00000015947.
GeneID	14129.
KEGG	mmu:14129.
Organism-specific databases
CTD	14129.
MGI	MGI:95498. Fcgr1.
Phylogenomic databases
eggNOG	NOG46371.
GeneTree	ENSGT00560000076958.
HOGENOM	HOG000251632.
HOVERGEN	HBG051602.
InParanoid	P26151.
KO	K06498.
OMA	SEYHIAR.
Gene expression databases
Bgee	P26151.
CleanEx	MM_FCGR1.
Genevestigator	P26151.
GermOnline	ENSMUSG00000015947. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 3 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013151. Immunoglobulin. [Graphical view]
Pfam	PF00047. ig. 1 hit. [Graphical view]
SMART	SM00409. IG. 2 hits. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	285222.
SOURCE	Search...

Entry information

Entry name

FCGR1_MOUSE

Accession

Primary (citable) accession number: P26151

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program