P26150 (3BHS3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3

Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type III
Short name=3-beta-HSD III

Including the following 2 domains:

1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
   EC=1.1.1.145
   Alternative name(s):
   3-beta-hydroxy-5-ene steroid dehydrogenase
   Progesterone reductase
2. Steroid Delta-isomerase
   EC=5.3.3.1
   Alternative name(s):
   Delta-5-3-ketosteroid isomerase

Gene names

Name:

Hsd3b3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity	A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue specificity	Liver and kidney. Greater expression in liver.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI
Molecular function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro steroid delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 373	372	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3		PRO_0000087782
Regions
	Transmembrane	288 – 308	21	Helical; Potential
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26150 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6F320FF1707974A6	FASTA	373	42,031
	10 20 30 40 50 60 MPGWSCLVTG AGGFLGQRII QLLVQEKDLE EIRVLDKVFK PETREQFFNL GTSIKVTVLE 70 80 90 100 110 120 GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV NLKGTQNLLE ACIQASVPAF 130 140 150 160 170 180 IFSSSVDVAG PNSYKDIVLN GHEDEHREST WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL 190 200 210 220 230 240 QTCALRPMCI YGERSQFLSN TIIKALKNKF ILRGGGKFST ANPVYVGNVA WAHILAARGL 250 260 270 280 290 300 RNPKKSPNIQ GEFYYISDDT PHQSYDDLNY TLSKEWGFCL NSRWYLPVPI LYWLAFLLET 310 320 330 340 350 360 VSFLLSPIYR YIPPFNRHLV TLTASTFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL 370 VEQHRETLDT KSQ

References

[1]	"Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes." Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H. Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed: 1924345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77015 mRNA. No translation available.
IPI	IPI00111855.
RefSeq	NP_001155214.1. NM_001161742.1. NP_001155215.1. NM_001161743.1. NP_001155216.1. NM_001161744.1. NP_001155217.1. NM_001161745.1.
UniGene	Mm.158717.
3D structure databases
ProteinModelPortal	P26150.
SMR	P26150. Positions 3-360.
ModBase	Search...
Protein-protein interaction databases
STRING	P26150.
Proteomic databases
PRIDE	P26150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000090743; ENSMUSP00000088246; ENSMUSG00000062410. ENSMUST00000107018; ENSMUSP00000102632; ENSMUSG00000062410. ENSMUST00000107019; ENSMUSP00000102633; ENSMUSG00000062410.
GeneID	15494.
KEGG	mmu:15494.
Organism-specific databases
CTD	15494.
MGI	MGI:96235. Hsd3b3.
Phylogenomic databases
eggNOG	roNOG12122.
GeneTree	ENSGT00550000074557.
HOGENOM	HBG755066.
HOVERGEN	HBG000014.
InParanoid	P26150.
OMA	TFSAVIN.
OrthoDB	EOG4K3KWG.
PhylomeDB	P26150.
Gene expression databases
ArrayExpress	P26150.
Bgee	P26150.
Genevestigator	P26150.
GermOnline	ENSMUSG00000062410. Mus musculus.
Family and domain databases
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

3BHS3_MOUSE

Accession

Primary (citable) accession number: P26150

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program